• Title/Summary/Keyword: Growth rates

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Effect of Microsparged Aeration on Oxygen Transfer Rate and Cell Viability in Mammalian Cell Culture Bioreactor (동물 세포 반응기에서의 초미세 통기법이 산소 전달 속도와 세포 생존율에 미치는 영향)

  • 김정모;장건희;최춘순;김정회
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.240-247
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    • 2001
  • The effect of microsparged aeration in mammalian cell bioreactor on the oxygen transfer rate and cell viability was studied. The microspargers with differ- ent micron-sized pores were used to supply oxygen to the medium. The oxygen transfer coefficients (k$_{L}$a) measured in the bioreactor were markedly increased, which is due to the increase of the contacting area between air bubbles and liquid medium when the pore size of microsparger decreases. When the impellers of two different types (square-pitch marine impeller and $45^{\circ}$ pitched flat blade impeller) were used for agitation, the k$_{L}$a values were slightly higher with the marine impeller than with the blade impeller. The detrimental effect of direct gas sparging with microsparger on mammalian cells was investigated in bubble columns with various air flow rates and different pore sized microspargers. The first-order cell death rate constant ($k_{d}$ /7) was shown to be directly proportional to the air flow rate and inversely proportional to the pore size. During the cultivation of hybridoma cells using microsparger with the pore size of $0.57\mu$m in the mammalian cell culture bioreactor, the continuous sparging caused the cell death and suppressed the cell growth. However, cells grew normally and cell viability was maintained above 90% in the logarithmic phase when the air was intermittently sparked in order to maintain the dissolved oxygen level above 20%.

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Site Characteristics and Carbon Dynamics of the Gwangneung Deciduous Natural Forest in Korea

  • Lim, Jong-Hwan;Shin, Joon-Hwan;Kim, Choonsig;Oh, Jeong-Soo
    • The Korean Journal of Quaternary Research
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    • v.17 no.2
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    • pp.163-163
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    • 2003
  • The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1m) in KEF, we have established a permanent plot and a flux tower, and the site was registered as a KLTER site and also a KoFlux site. In this study, we aimed to present basic ecological characteristics and synthetic data of carbon budgets and flows, and some monitoring data which are essential for providing important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function. We made a stemmap of trees in 1 ha plot and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 m2/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer. Seasonal changes in LAI were measured by hemispherical photography at the l.2m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. Litterfall was collected in circular littertraps (collecting area: 0.25m2) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30cm30cm nylon bags with l.5mm mesh size. Total annual litterfall was 5,627 kg/ha/year and leaf litter accounted for 61% of the litterfall. The leaf litter quantity was highest in Quercus serrata, followed by Carpinus laxiflora and C. cordata, etc. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. And then we have drawn a schematic diagram of carbon budgets and flows in each compartment of the KEF site.

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$CO_2$ and Water Vapor Flux Measurement by Eddy Covariance Method in a Paddy Field in Korea (한반도 논에서의 에디공분산 방법에 의한 $CO_2$와 수증기 플럭스 관측)

  • Lee Jeongtaek;Lee Yangsoo;Kim Gunyeob;Shim Kyomoon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.1
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    • pp.45-50
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    • 2005
  • This study was conducted to measure and understand the exchange of CO₂ and water in a rice canopy. Eddy covariance system was installed on a 10m tower along with other meteorological instruments. CO₂ flux and surface energy balance were measured throughout the whole growing season in 2003 over a typical paddy field in Icheon, Korea. During the early growth stage in May and June, most of net radiation was partitioned to latent heat flux with daytime Bowen ratio of 0.3 to 0.7. Evapotranspiration (i.e., daily integrated latent heat flux) typically ranged from 3 to 4 mm d/sup -1/, with even higher rates on sunny days. Daily integrated net ecosystem exchange (NEE) of CO₂ increased with increasing solar radiation and leaf area index (LAI). The NEE was especially high during the stages of young panicle formation and heading. On 1 June 2003, when the rice field was flooded, it was a weak sink of atmospheric CO₂ with an uptake rate of 9.1 gm/sup -2/d/sup -1/. Despite frequent rainy and cloudy conditions in summer, maximum NEE of 36.2 gm/sup -2/d/sup -1/ occurred on 31 July prior to heading stage. As rice crop senesced after early September, the NEE decreased.

Foundation of Management Innovation and a Success Model of Micro Enterprise by Increasing Entrepreneurship and Organization Relationship Analysis

  • Suh, Geun-Ha;Hong, Yong-Woong;Jin, Soon-Ae;Jo, Geum-Je
    • Journal of Distribution Science
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    • v.10 no.3
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    • pp.31-42
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    • 2012
  • After the rapidly changing business management environment and financial crisis, high prices and domestic recession in Korea posed threats to the business survival of small and medium enterprises (SMEs). This paper looks at the potential of micro business owners for sustainable development: those who have a spirit of entrepreneurship and the potential to be a hidden champion. Our study of micro business values uses structure equation modeling by LISREL. We have done so in order to understand the foundation of management innovation and success model of micro enterprise by increasing the entrepreneurship and management performance relationship analysis. A micro-enterprise is a type of small business, found only in Korea, often registered as having ten or fewer employees. We examine the successful type of hidden champions and the influence of entrepreneurship on start-ups in business. As compared to past research on the SME entrepreneurship, this study segments small businesses even further. Small business entrepreneurship was classified into three forms that are most appropriate for the Korean situation today: innovation, risk-taking, and pro-activeness. This research is meaningful as it is Korea's first empirical analysis on four business types: wholesale and retail sales, food and lodging business, service business, and manufacturing business. Thus far, research on small business entrepreneurship was carried out using small-scale investigative analysis. However, this research attempted to develop a model that can explain a cause-effect relation of the motivational level when it comes to the difference of entrepreneurship by each business type and small business start-up success factors. Various conceptual and operational definitions could be developed in a diverse and precise manner. Independent variables that are related to the success of small businesses can be developed additionally to examine the success factors related to the systematization in detailed manner. The research showed that the innovation, risk-taking and pro-activeness of the manifested difference in the degree of perception depending on the type of small businesses. Among the four business types, the ones that manifested the highest successful start-up rates were food, service, wholesale and retail sales and manufacturing. Results after conducting the regression analysis are shown on, which proves that the small business entrepreneurship exerts direct effect on the financial management performance of small businesses. In other words, small business entrepreneurship exerts a positive effect on the small business financial success and management performance. The R2 value is 0.61. It is possible to know that the perception of systematization and variables on attitude explains the 61% of the success for small self-employed businesses management performance. We define start-up key factors that are helpful to achieve internal growth of firms by finding business survival strategies. The results also focus on Korean government policy for micro enterprise and small business support.

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Health Risk Behaviors Affecting the Process of Pregnancy (임신경과에 영향을 미치는 건강위험행위)

  • Park, Chai-Soon;Mun, Mi-Seon;Hong, Gin-Hee;Lee, Jeoung-Eun
    • Women's Health Nursing
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    • v.6 no.4
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    • pp.549-565
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    • 2000
  • Comprehensive review of the literature was conducted to determine 1) selected risk factors and its impact that affect pregnancy outcome such as smoking, alcohol consumption, and substance abuse 2) these factors can facilitate future strategies for health promotion and prevention for both pregnant women and fetus. Review of literature were extracted from searching MEDLINE(1966 - Oct. 2000). CINAHL (1982 - Oct. 2000) and the domestic literature. The following factors were identified: 1. The effects of risk behaviors on pregnancy. ${\cdot}$Maternal smoking was associated with the occurrence of premature or LBW delivery, fetal growth retardation, extremities defects, heart defects and sudden infant death syndrome. ${\cdot}$Maternal alcohol consumption was associated with spontaneous abortion, premature or LBW delivery, morphologic/neurologic problems, especially fetal alcohol syndrome. ${\cdot}$Heroin was associated with withdrawal after birth in which were born to heroine addicts for gestational age and lung maturation in animal studies. ${\cdot}$Cocaine was associated with spontaneous abortion, abruptio placenta and a poor response to environmental stimuli. ${\cdot}$So far, the effects of caffeine on pregnancy was controversial, but severe caffeine consumption was associated with premature or LBW delivery, spontaneous abortion, still birth and dystocia. 2. Intervention methods and its effects identified were as follows ${\cdot}$Conducted intervention for smoking, alcohol and drug consumption were single or combined. ${\cdot}$Intervention methods were counseling, phone contact, mailing, use of educational videotape, booklet, support person and alternatives such as nicotine patch. ${\cdot}$The interventions increased the rates of smoking cessation during pregnancy and awareness of the risk of drug consumption, and decreased amount of alcohol consumption. ${\cdot}$The intervention outcome found positive effect on birth weight and length. 3. Our recommendations were as follows ${\cdot}$The personal and social cognition should be enhanced through education and the mass media. ${\cdot}$It's necessary to educate and give information of preconceptional care, planned pregnancy and early prenatal care for optimal pregnancy outcome. ${\cdot}$It's necessary to develop comprehensive assessment tool which is reliable and valid on smoking, alcohol consumption and substance abuse to identify supportive or interventional program.

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Effects of soluble silicon on development powdery mildew(Sphaerotheca fuliginea) in cucumber plants (규소 시용에 의한 오이 흰가루병 발병억제)

  • Lee, Jung-Sup;Yiem, Myeong-Sun
    • The Korean Journal of Pesticide Science
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    • v.4 no.2
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    • pp.37-43
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    • 2000
  • Effects of silicon application on development of colonies of Sphaerotheca fuliginea were examined. Cucumber plants were applied with nutrient solutions amended with different concentrations of soluble silicon and selected leaves were inoculated with known concentrations of conidia of the pathogen. Colony number per leaf, colony area per leaf, and germination rate of conidia of S. fuliginea collected from the inoculated leaves were reduced as silicate concentrations in the nutrient solutions increased from 0.05 to 4.10 mM. The increase in resistance of plants to mildew infection was apparently due to silicate accumulation in leaves, and there was no correlation between cation or ionic strength effects and the silicate treatments. Silicate treatment in growth medium remarkably suppressed powdery mildew development on cucumber. Colonies of mildew fungus were visible with over approx. 38.3% of the mature leaf surface, while that of the leaves in high Si plants was 2.3% observed at 51 days after transplanting. No significant differences were observed between 1.7 mM and 3.4mM silicate treatments. Conidial germination rates were significantly reduced by increasing Si amendments. Conidial germination ranged from 14.7 to 20.3% for plants grown in low Si solution(<1.40 mM), and from 9.0 to 12.4% for plants grown in high Si solution(>1.8 mM). Foliar applications of Si with ${\geq}$ 17.0 mM decreased the number of powdery mildew colonies. Persistence of Si foliar sprays effects on cucumber demonstrated that the 17 mM Si spray applied 4 days before inoculation with S. fuliginea reduced mildew colony formation. The relationship was positive and linear.

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the Impact of Medium Sized Firm's Knowledge and Industry Dynamism on Firm Performance (중기업의 지식자산과 산업의 역동성이 기업성과에 미치는 영향)

  • Park, Sun-Young
    • Journal of Korea Technology Innovation Society
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    • v.10 no.3
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    • pp.509-530
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    • 2007
  • This paper examined the relationship between innovation and firm performance by integrating industry competitive context and firm-level knowledge constructs. After controlling for firm site, age, and the presence of a union, cross-section analysis of survey data from 1,419 medium sized manufacturing firms yielded following findings. The first was the expected positive relationship between firm-level innovation and firm knowledge and also industry dynamism, as measured by the intensity of industry-level R&D. This results indicate that industries with greater aggregate levels of R&D intensity are home to higher rates of firm-level innovative activity and managers must increase their numbers of technical staff and the level of training. But the interaction between firm knowledge and industry dynamism was non-significant. Second, innovation was not significantly related to firm performance, as measured by revenue growth. This relationship was not moderated by industry dynamism and firm level knowledge. In high and low technology sectors, the relationship between innovation and performance was non-significant, consistent with the full-sample analysis. The results suggest that the effects of firm-level knowledge assets and investments in training don't work in different ways in different industry settings. This research used three control variables to analyze innovation and firm performance. Firm age was negatively associated with firm performance and did not significantly predict innovation. Firm size was positively associated with innovation and performance in the low-technology sector. The presence of a labor union was not a significant with respect to innovation.

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Korean Customer Attitudes Towards SNS Shopping

  • Cho, Young-Sang;Heo, Jeong-Yoon;Youn, Myoung-Kil
    • Journal of Distribution Science
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    • v.10 no.8
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    • pp.7-14
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    • 2012
  • As a new format of retailing, social shopping on SNS has rapidly grown in recent. Although there is much literature associated with customer behaviours in the academic world, little attention has been paid to identifying the shopping patterns of SNS shoppers. This paper will, thus, identify how perceived value has an impact on the buying intention of SNS shoppers, after illustrating what kind of factor influences the formation process of perceived value in the Korean marketplace. Given that SNS shoppers are for the most part 20s as well as 30s, the authors handed out questionnaires to them. Furthermore, based on literature review results, the conceptualised research model was developed. Despite lack of literature, the authors developed five constructs like price reduction, quantity- and time-limited message, product ranges, information-sharing, and required number of shoppers. The researchers made a considerable effort to identify the relationship between research concepts and each variable, based on a few research analysis methods such as frequency analysis, the Varimax rotation technique used orthogonal rotation, Cronbach's Alpha, PCA (Principle Component Analysis), and the like. Amongst the 5 variables used to measure the degree of influences on the perceived value as a social shopping characteristic, it has been evident that price cut, required minimum shoppers, product variety, and information-sharing have a positive impact on the perceived value formation processes of SNS customers. Also, this research implies that SNS retailers can differentiate themselves from other retailers by differently using the above factors. From a practitioner's point of view, these factors should be strategically used to increase the social shopping opportunities of SNS users. It is, furthermore, evident that the perceived value formed by the above 4 factors have played an important role in the buying decision process of SNS customers. In a sense, whether customers are aware of higher price cut rates, information-sharing, required minimum shoppers, and product variety has a positive impact on making buying decisions. From a retailer's point of view, online shopping mall operators are able to use blog as well as twitter to improve the buying intention as a marketing tool of social network, because the business activities provided by social shopping retailers, like the rapid, accurate responses to customer requirements, the provision of a variety of information, and the communications between customers are closely related to buying intentions. There are a few research limitations to conduct this empirical research. It was not easy to review prior papers, due to its lack. In spite of the increasing number of SNS shoppers in Korea, little research attention has been paid to this kind of research topic by academicians, because buying products or services through SNS is in its infancy. With regard to research populations, it would be difficult to generalise the research findings in Korea, owing to unbalanced respondent distribution. Considering the above research limitations as well as the growth of social shopping, many authors should pay considerable attention to SNS-related issues in the future, and develop the more sophisticated criteria to measure the characteristics of SNS shoppers.

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How does the CAP System Influence the Structure of Gambling Industry in Korea (사행산업 매출총량제가 사행산업 구조에 미친 영향 분석)

  • Choi, Seong-Rak;Park, Jun-Hwi
    • The Journal of the Korea Contents Association
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    • v.18 no.5
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    • pp.34-45
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    • 2018
  • In Korea, there are a lot of regulation on gambling industry. Among them, the most unique regulation is the CAP system. The purpose of CAP system is to minimize the social side effects of gambling industry. For that, National Gambling Control Commission controls the maximum number of business premises of gambling companies. The CAP system is a means of preventing excessive expansion of the gambling industry. The CAP system has lasted since 2009. This study investigate how the CAP system influence the structure of gambling industry. In result, after the CAP system carried out, the growth rates of gambling industry are similar to those of national GDP. And before the CAP system, the HHI index and CR index were decreasing trend. However, after the CAP system, the HHI index and CR index were not decreasing. That means the structure of gambling industry has been stable and settled. And there were few variation among the gambling companies in sales. In conclution, the CAP system influenced the gambling industry to be stable. There were few competition among the gambling companies in Korea. And the structure of gambling industry has been settled.

Removal of Endocrine Disrupting Chemicals in Wastewater by Nitrifying Sludge (질산화 슬러지에 의한 폐수 중의 내분비계 장애물질 제거)

  • Lim, Kyoung Jo;Hong, Soon Ho;Chung, Jin Suk;Yoo, Ik-Keun
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.775-780
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    • 2009
  • The efficacy of nitrifying sludge existed in biological nutrient removal process was examined for possible removal of endocrine disrupting chemical(EDC) in the effluent of wastewater treatment plant. Some of ammonia oxidizing bacteria causes ammonia oxidation mediated by ammonia monooxygenase(AMO) activity, which has low substrate specificity resulting in cometablic degradation of several chemicals. In this study, the removal of three model EDCs such as bisphenol A(BPA), nonylphenol(NP) and dibutyl phthalate(DBP) was studied in batch cultures using nitrifying sludge, BOD-oxidizing sludge with low nitrifying activity, and sterilized sludge. Nitrifying sludge showed higher initial removal rates in all batches of three EDCs when it was fed with ammonium as an energy source. The acclimation time was required for the removal of EDCs in batches using BOD-oxidizing sludge or nitritefed nitrifying sludge. That retardation seemed to attribute to the slow growth of cells using the EDCs while ammonium-fed nitrifying sludge could degrade EDCs through simultaneous cooxidation with ammonia oxidation. Sterilized sludge was also tested under the same conditions in order to find the contribution of physical adsorption to the removal of EDCs. About 10~20% of initial EDCs dose was removed when using sterilized sludge. Thus the biological activity is likely to play major role for the degradation of BPA, NP, and DBP rather than the physical adsorption from wastewater.