• Journal of Ocean Engineering and Technology
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• v.23 no.2
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• pp.69-73
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• 2009

The objective of this study was to obtain a better understanding of the delayed hydride cracking (DHC) of Zr-2.5Nb alloy. The DHC model has some defects: first, it cannot explain why the DHC velocity (DHCV) becomes constant regardless of an applied stress intensity factor, even though the stress gradient is affected by the applied stress intensity factor at the notch tip. Second, it cannot explain why the DHCV has a strong dependence on the method of approaching the test temperature by a cool-down or a heating-up, even under the same stress gradient, and third, it cannot predict any hydride size effect on the DHC velocity. The DHC tests were conducted on Zr-2.5Nb compact tension specimens with the test temperatures reached by a heating-up method and a cool-down method. Crack velocities were measured in hydrided specimens, which were cooled from solution-treatment temperatures at different rates by being furnace-cooled, water-quenched, and liquid nitrogen-quenched. The resulting hydride size, morphology, and distributions were examined by optical metallography. It was found that fast cooling rates, which produce very finely dispersed hydrides, result in higher crack growth rates. This different DHC behavior of the Zr-2.5Nb tube with the cooling rate after a homogenization treatment is due to the precipitation of the $\gamma$-hydrides only in the water-quenched Zr-2.5Nb tube. This experiment will provide supporting evidence that the terminal solid solubility of a dissolution (TSSD) of $\gamma$-hydrides is higher than that of $\delta$-hydrides.

• Journal of computational fluids engineering
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• v.21 no.2
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• pp.12-24
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• 2016

The standard ${\kappa}-{\varepsilon}$ and realizable ${\kappa}-{\varepsilon}$ models are adopted to improve the prediction performance on the recirculating flow. In this paper, the backward facing step flows are used to assess the prediction performance of the recirculation zone. The model constants of turbulence model are obtained by the experimental results and they have a different value according to the flow. In the case of an isotropic flow situation, decaying of turbulent kinetic energy should follow a power law behavior. In accordance with the power law, the coefficients for the dissipation rate of turbulent kinetic energy are not universal. Also, the other coefficients as well as the dissipation coefficient are not constant. As a result, a suitable coefficients can be varied according to each of the flow. The changes of flow over the backward facing step in accordance with model constants of the ${\kappa}-{\varepsilon}$ models show that the reattachment length is dependent on the growth rate(${\lambda}$) and the ${\kappa}-{\varepsilon}$ models can be improved the prediction performance by changing the model constants about the recirculating flow. In addition, it was investigated for the curvature correction effect of the ${\kappa}-{\varepsilon}$ models in the recirculating flow. Overall, the curvature corrected ${\kappa}-{\varepsilon}$ models showed an excellent prediction performance.

• Journal of Soil and Groundwater Environment
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• v.21 no.5
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• pp.8-15
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• 2016

Bioremediation, which uses microbes to degrade most organic pollutants in soil and groundwater, can be used in solving environmental issues in various polluted sites. In this research, a wind-driven bioventing system is built to degrade about 20,000 mg/kg of high concentration diesel pollutants in soil-pollution mode. The wind-driven bioventing test was proceeded by the bioaugmentation method, and the indigenous microbes used were Bacillus cereus, Achromobacter xylosoxidans, and Pseudomonas putida. The phenomenon of two-stage diesel degradation of different rates was noted in the test. In order to interpret the results of the mode test, three microbes were used to degrade diesel pollutants of same high concentration in separated aerated batch-mixing vessels. The data derived thereof was input into the Haldane equation and calculated by non-linear regression analysis and trial-and-error methods to establish the kinetic parameters of these three microbes in bioventing diesel degradation. The results show that in the derivation of μ_m (maximum specific growth rate) in biodegradation kinetics parameters, K_s (half-saturation constant) for diesel substance affinity, and K_i (inhibition coefficient) for the adaptability of high concentration diesel degradation. The K_s is the lowest in the trend of the first stage degradation of Bacillus cereus in a high diesel concentration, whereas K_i is the highest, denoting that Bacillus cereus has the best adaptability in a high diesel concentration and is the most efficient in diesel substance affinity. All three microbes have a degradation rate of over 50% with regards to Pristane and Phytane, which are branched alkanes and the most important biological markers.

• Journal of Soil and Groundwater Environment
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• v.21 no.6
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• pp.87-100
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• 2016

Biochar, which has high alkalinity, has widely studied for amendment of soil that contaminated with heavy metals. The aim of this study is assessment of amendment for arsenic and heavy metals contaminated acidic agricultural soil using biochar that derived from buffalo weed (A. trifida L. var. trifida). Pot experiments were carried out including analysis of soil solution, contaminants fractionation, soil chemical properties and plant (lettuce) uptake rate. Arsenic and heavy metals concentrations in soil solution showed relatively low in biochar added experiments when compared to the control. In the heavy metals fractionation in soil showed decrease of exchangeable fraction and increase of carbonates fraction; however, arsenic fractionations showed constant. Soil chemical properties indicated that biochar could induce recovery of soil quality for plant growth in terms of soil alkalinity. However, phosphate concentration in biochar added soil decreased due to Ca-P precipitation by exchangeable calcium from biochar. Arsenic and heavy metals uptake rate of plant in the amended experiment decreased to 50% when compared to the control. Therefore biochar derived from buffalo weed can be used as amendment material for agricultural soil contaminated with arsenic and heavy metals. Precipitation of As-Ca and metal-carbonates are major mechanisms for soil amendment using char.

• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.204-211
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• 2011

To yield high concentrations of protein expressed by genetically modified Escherichia coli, it is important that the bacterial strains are cultivated to high cell density in industrial bioprocesses. Since the expressed target protein is mostly accumulated inside the E. coli cells, the cellular product formation can be directly correlated to the bacterial biomass concentration. The typical way to determine this concentration is to sample offline. Such manual sampling, however, wastes time and is not efficient for acquiring direct feedback to control a fedbatch fermentation. An E. coli K12-derived strain was cultivated to high cell density in a pressurized stirred bioreactor on a pilot scale, by detecting biomass concentration online using a capacitance probe. This E. coli strain was grown in pure minimal medium using two carbon sources (glucose and glycerol). By applying exponential feeding profiles corresponding to a constant specific growth rate, the E. coli culture grew under carbon-limited conditions to minimize overflow metabolites. A high linearity was found between capacitance and biomass concentration, whereby up to 85 g/L dry cell weight was measured. To validate the viability of the culture, the oxygen transfer rate (OTR) was determined online, yielding maximum values of 0.69 mol/l/h and 0.98mol/l/h by using glucose and glycerol as carbon sources, respectively. Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures.

• KSBB Journal
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• v.32 no.2
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• pp.117-123
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• 2017

Maple tree is a useful medical plant for obtaining bioactive materials such as pharmaceutics, cosmetics, food additive, etc., and there are 16 species of native maple trees in Korea. In this study, we evaluated the antioxidant activity of sap and crude extracts of Acer mono Max, a representative maple species. The crude extracts were obtained by solvent extraction (water, ethanol, and ethyl acetate) from its branches (bark and xylem). The phenolic contents and radical scavenging capacities of the extracts and the sap were evaluated in terms of half maximal effective concentration ($EC_{50}$) and kinetics by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanol extracts showed the highest extraction yield, phenolic contents and antioxidant activity, and bark extracts showed better antioxidant activity than xylem extracts. The antioxidant activity of the sap was very low, but the $EC_{50}$ of ethanol and ethyl acetate extracts ranged from 68 to $79{\mu}g/mL$, similar to that ($60{\mu}g/mL$) of the control, butylated hydroxytoluene (BHT). The DPPH radical scavenging rate ($220{\sim}760{\mu}M/min$) and the second-order reaction rate constant ($6.48{\sim}7.04L/g{\cdot}min$) of these extracts were better than those of BHT ($55{\sim}370{\mu}M/min$ and $3.60L/g{\cdot}min$). These results suggest that A. mono Max. is one of the useful bioresources for obtaining antioxidant biologically active substances, and it is possible to obtain physiologically active substances from by-product of its pruning while minimizing the effect on the growth of the tree.

• Journal of the Korean Society for Heat Treatment
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• v.3 no.3
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• pp.13-20
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• 1990

SCM 21 and D.P steel were carburized in vacuum using prophane at a temperature ranging from 1228k to 1308k under a gas pressure ranging from 21.3kpa to 61.8kpa, and the following results were obrained. 1) D.P. steel has considerable efficiency in depressing the grain growth during the high temperature carburizing and it has fine structure even at 1268k for 14.4ks when carburizied. Therefore this steel is expected to be suitable for vacuum carburizing at a high temperature. 2) Case depth was increased as the carburizing temperature increases and it was 3.2mm at max, temperature of 1308k, for max, time of 14.4ks and under max, pressure of 61.8kpa. Thus vacuum-carburizing was considered effective for the materials which need case depth, which is necessary for machine structure use. 3) The rate of case depth of SCM 21 was faster than D.P. steel under same carburizing conditions and the increasing rate of the case depth was constant. 4) Case depth was increased as the gas pressure becomes high under same carburizing temperature. 5) Case carbon concentration, $C_s$, of SCM 21 obeys to a formula, $$C_s=kt^{1/2}+C_0$$ Where k is $2.15{\times}10^{-2}$($wt%.S^{-1/2}$) and this value is a little bit lower than that of SNCM 815.

• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.240-247
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• 2001

The effect of microsparged aeration in mammalian cell bioreactor on the oxygen transfer rate and cell viability was studied. The microspargers with differ- ent micron-sized pores were used to supply oxygen to the medium. The oxygen transfer coefficients (k$_{L}$a) measured in the bioreactor were markedly increased, which is due to the increase of the contacting area between air bubbles and liquid medium when the pore size of microsparger decreases. When the impellers of two different types (square-pitch marine impeller and $45^{\circ}$ pitched flat blade impeller) were used for agitation, the k$_{L}$a values were slightly higher with the marine impeller than with the blade impeller. The detrimental effect of direct gas sparging with microsparger on mammalian cells was investigated in bubble columns with various air flow rates and different pore sized microspargers. The first-order cell death rate constant ($k_{d}$ /7) was shown to be directly proportional to the air flow rate and inversely proportional to the pore size. During the cultivation of hybridoma cells using microsparger with the pore size of $0.57\mu$m in the mammalian cell culture bioreactor, the continuous sparging caused the cell death and suppressed the cell growth. However, cells grew normally and cell viability was maintained above 90% in the logarithmic phase when the air was intermittently sparked in order to maintain the dissolved oxygen level above 20%.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.355-363
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• 2015

The present work quantified the growth of young biofilm in a model distribution system that was fed with chlorinated drinking water at a hydraulic retention time of 2 h. Bacterial biofilms grew on the surface of polyvinyl chloride (PVC) slides at a specific growth rate of $0.14{\pm}0.09day^{-1}$ for total bacteria and $0.16{\pm}0.08day^{-1}$ for heterotrophic bacteria, reaching $3.1{\times}10^4cells/cm^2$ and $6.6{\times}10^3CFU/cm^2$ after 10 days, respectively. The specific growth rates of biofilm-forming bacteria were found to be much higher than those of bulk-phase bacteria, suggesting that biofilm bacteria account for a major part of the bacterial production in this model system. Biofilm isolates exhibited characteristic kinetic properties, as determined by ${\mu}_{max}$ and $K_S$ values using the Monod model, in a defined growth medium containing various amounts of acetate. The lowest ${\mu}_{max}$ value was observed in bacterial species belonging to the genus Methylobacterium, and their slow growth seemed to confer high resistance to chlorine treatment (0.5 mg/L for 10 min). $K_S$ values (inversely related to substrate affinity) of Sphingomonas were two orders of magnitude lower for acetate carbon than those of other isolates. The Sphingomonas isolates may have obligate-oligotrophic characteristics, since the lower $K_S$ values allow them to thrive under nutrient-deficient conditions. These results provide a better understanding and control of multi-species bacterial biofilms that develop within days in a drinking water distribution system.

• Journal of the Microelectronics and Packaging Society
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• v.18 no.1
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• pp.29-33
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• 2011

The current density in copper electroplating is directly related with the productivity and then to increase the productivity, the increase in current density is required. To obtain the high mass flow rate, rotating disk electrode(RDE) was employed. High rotational speed in RDE can increase the mass flow rate and then high speed electroplating was possible using RDE to control mass flow. Two types of cathode were used. One is RDE and another is rotating cylindrical electrode(RCE). A constant-current, constant-voltage and linear sweep voltammetry were applied to investigate current and voltage relationship. The maximum current density without evolution of hydrogen gas was increased with rotational speed. Over 400 rpm, maximum current density was higher than 1000 A/$m^2$. The diffusion coefficients of copper calculated from the slope of the plots are $5.5{\times}10^6\;cm^2\;s^{-1}$ at $25^{\circ}C$ and $10.5{\times}10^6\;cm^2\;s^{-1}$ at $62^{\circ}C$. The stable voltage without evolution of hydrogen gas was -0.05 V(vs Ag/AgCl). Additives were added to prevent dendritic growth on cathode deposits. The surface roughness was analyzed with UV-Vis Spectrophotometer. The reflectance of the copper surface over 600 nm was measured and was related with the surface roughness. As the surface roughness improved, the reflectance was also increased.