• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.896-902
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• 2008

Yukmijihwang-tang(YM) is used in Oriental medicine for treatments of diverse systemic symptoms including neurological dosorders. The present study was performed to examine potential effects of YM on growth-promoting activity of injured sciatic nerve axons. YM treatment in the injured sciatic nerve induced enhanced distal elongation of injured axons when measured 3 and 7 days after injury. Retrograde tracing of sciatic nerve axons showed YM-mediated increases in the number of DiI-labeled dorsal root ganglion (DRG) sensory neurons and spinal cord motor neurons at 3 days after injury. Hoechst nuclear staining showed that non-neuronal cell population was largely elevated by YM treatment in distal nerve area undergoing axonal regeneration. Furthermore, phospho-Erk1/2 protein levels were upregulated by YM treatment in the injured nerve area. These data suggest that YM may play a role in facilitated axonal regeneration in injured peripheral nerves. Further investigations of individual herbal components would be useful to explore effective molecular components and develop therapeutic strategies.

• Korean Journal of Pharmacognosy
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• v.41 no.4
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• pp.270-274
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• 2010

Obesity is caused from an imbalance between energy intake and expenditure, which may lead to pathologic growth of adipocytes and accumulation of fat in tissue. We examined the inhibitory effects of Eriobotrya japonica leaf and seed extracts on lipid absorption in vitro and fat accumulation during the differentiation of 3T3-L1 to adipocytes. 3T3-L1 preadipocytes were stimulated with DMEM media containing 10% FBS, 0.5 mM 3-isobuthyl-1-methyxanthine (IBMX), $5\;{\mu}g/ml$ insulin, and $1\;{\mu}g/ml$ dexamethasone for differentiation to adipocytes. E. japonica leaf extract at concentration of 0.5 or 1 mg/ml inhibited pancreatic lipase activity. The cell viability of 3T3-L1 adipocytes slightly reduced about 3% by treatment of E. Japonica leaf and seed extracts. The leaf and seed extracts of E. japonica effectively inhibited the accumulations of lipid droplet and expression of $C/EBP{\alpha}$ promoting adipogenesis. Thus, this data suggest that E. japonica leaf and seed extracts inhibit fat accumulation through regulation of $C/EBP{\alpha}$, and leaf extract is more effective in lipid absorption and adipogenesis than seed extract.

• The Plant Pathology Journal
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• v.33 no.2
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• pp.148-162
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• 2017

As a step towards discovering novel pathogenesis-related proteins, we performed a genome scale computational identification and characterization of secreted and transmembrane (TM) proteins, which are mainly responsible for bacteria-host interactions and interactions with other bacteria, in the genomes of six representative Burkholderia species. The species comprised plant pathogens (B. glumae BGR1, B. gladioli BSR3), human pathogens (B. pseudomallei K96243, B. cepacia LO6), and plant-growth promoting endophytes (Burkholderia sp. KJ006, B. phytofirmans PsJN). The proportions of putative classically secreted proteins (CSPs) and TM proteins among the species were relatively high, up to approximately 20%. Lower proportions of putative type 3 non-classically secreted proteins (T3NCSPs) (~10%) and unclassified non-classically secreted proteins (NCSPs) (~5%) were observed. The numbers of TM proteins among the three clusters (plant pathogens, human pathogens, and endophytes) were different, while the distribution of these proteins according to the number of TM domains was conserved in which TM proteins possessing 1, 2, 4, or 12 TM domains were the dominant groups in all species. In addition, we observed conservation in the protein size distribution of the secreted protein groups among the species. There were species-specific differences in the functional characteristics of these proteins in the various groups of CSPs, T3NCSPs, and unclassified NCSPs. Furthermore, we assigned the complete sets of the conserved and unique NCSP candidates of the collected Burkholderia species using sequence similarity searching. This study could provide new insights into the relationship among plant-pathogenic, humanpathogenic, and endophytic bacteria.

• Biomolecules & Therapeutics
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• v.23 no.2
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• pp.128-133
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• 2015

Although coffee is known to have antioxidant, anti-inflammatory, and antitumor properties, there have been few reports about the effect and mechanism of coffee compounds in colorectal cancer. Heat shock proteins (HSPs) are molecular chaperones that prevent cell death. Their expression is significantly elevated in many tumors and is accompanied by increased cell proliferation, metastasis and poor response to chemotherapy. In this study, we investigated the cytotoxicity of four bioactive compounds in coffee, namely, caffeine, caffeic acid, chlorogenic acid, and kahweol, in HT-29 human colon adenocarcinoma cells. Only kahweol showed significant cytotoxicity. Specifically, kahweol increased the expression of caspase-3, a pro-apoptotic factor, and decreased the expression of anti-apoptotic factors, such as Bcl-2 and phosphorylated Akt. In addition, kahweol significantly attenuated the expression of HSP70. Inhibition of HSP70 activity with triptolide increased kahweol-induced cytotoxicity. In contrast, overexpression of HSP70 significantly reduced kahweol-induced cell death. Taken together, these results demonstrate that kahweol inhibits colorectal tumor cell growth by promoting apoptosis and suppressing HSP70 expression.

• Journal of Technology Innovation
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• v.12 no.2
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• pp.67-97
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• 2004

Practical experience with technology implementation of the upgrading of very small village industries in India suggests that innovation failures are not merely a result of the lack of proper interaction between the users and suppliers of technologies under implementation, but also a result of adoption of the primitive conception of competitiveness in their practice of technology development. The approach of promoting the small producers to become individually competitive by using labour intensive, small-scale intermediate technologies is proving to be totally inadequate for the achievement of technological efficiency in a dynamic sense. Guided by a primitive notion of competitiveness, the suppliers of intermediated technologies are thus being led into limiting their technological efforts in the sectors of direct interest to the rural industrial clusters to the transitional objectives of mainly poverty alleviation. Consequently they have not been able to target the small producers of these village industries for the objectives of business growth. This paper posits that under competitive conditions the self-employed small producer has not only to come together for access to resources, but also has to emerge as a multi-sectoral collective of producers, co-operating in production. With the aim to draw lessons that are generic and have policy implications for the development of innovation systems for local economy based rural industrial clusters and value chains, the author analyses in this paper the experience of innovation in technological systems for the sectors of leather, fruits and vegetable processing and agro processing by the People's Science Movement with the help of the Ministry of Science and Technology and other sectoral ministries in India where rural poor were required to pool the resources and capabilities for raising the scale and scope of their collective production organization.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.30 no.4
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• pp.18-27
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• 1998

Biopulping is defined as the treatment of wood chips with lignin-degrading fungi prior to pulping. Fungal pretreatment prior to mechanical pulping reduces electrical energy requirements during refining or increases mill throughput, improves paper strength, reduces the pitch content, and reduces the environmental impact of pulping. Our recent work involved scaling up the biopulping process towards the industrial level, investigating both the engineering and economic feasibility. We envision the process to be done in either a chip-pile or silo-based system for which several factors need to be considered: the degree of decontamination, a hospitable environment for the fungus, and the overall process economics. Currently, treatment of the chips with low-pressure steam is sufficient for decontamination and a simple, forced ventilation system maintains the proper temperature, humidity, and moisture conditions, thus promoting uniform growth of the fungus. The pilot-scale trial resulted in the successful treatment of 4 tons of wood chips (dry weight basis) with results comparable to those on a laboratory. Larger, 40-ton trials were also successful, with energy savings and paper properties comparable with the laboratory scale. The overall economics of the process also look very favorable and can result in significant annual savings to the mill. Although the current research has focused on biopulping for mechanical pulping, it is also beneficial for sulfite chemical pulping and some applications to recycled fiber have been investigated.

• Journal of Agricultural Extension & Community Development
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• v.15 no.3
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• pp.499-531
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• 2008

Rural Traditional Theme Villages are one of the village-based businesses, which obtains excellent results. However, it also has brought negative results such as splits in locals due to income disparity, and ill-advised development which does not consider surrounding potential resources, etc. So, this study analyzes several problems of Rural Traditional Theme Villages, and proposes how Rural Traditional Theme Villages can be revitalized by benchmarking other successful cases. First, it is important to pursue differentiation of operating programs for Rural Traditional Theme Villages. Second, it is possible to give weight to elements which show efficiently rural atmosphere to enhance images of Rural Traditional Theme Villages. Third, expanding budget support and securing a new route for budget are definitely needed to cultivate self-growth capability of villages. Fourth, it is necessary to apply experts and facilities of local-based universities, cooperate with relevant organizations, and nurture local leaders by various means such as study in/visits to advanced regions at home and abroad and experts training. Localgovernment should be in charge of nurturing talented people. Fifth, it is necessary to reinvigorate rural economy by networking each Rural Traditional Theme Village, providing a wide range of information on diversified villages to people in cities, and promoting consumption. Moreover, securing immovable customers is important through networking city-rural exchange with city consumers and other organizations, and sisterhood relationship, membership businesses and events. As mentioned above, it is expected that Rural Traditional Theme Villages will be able to be developed continuously as a model for rural revitalization, which is stagnant, by expanding city-rural interchange.

• BMB Reports
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• v.34 no.2
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• pp.114-117
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• 2001

Cervi Parvum Cornu is widely used as a hemopoietic, tonifying, growth-promoting, cardiotonic, and immuno-modulating agent in Korea. In order to develop the quality control method of Cervi Parvum Cornu by the identification of the biological source or origin, the molecular approach was applied using PCR (polymerase chain reaction) and PCR-RFLF (PCR-restriction fragment length polymorphism) analysis. In the PCR analysis of the mitochondrial 12S rRNA gene and cytochrome b gene regions, no distinctive DNA bands from Cervidae (deer) antlers and Rangifer (reindeer) antlers were observed. However, when the amplified products in the mitochondrial cytochrome b gene region were subjected to restriction digestion with TaqI, Cervidae antlers showed an undigested state of 380 by band, differently from two bands of 230 by and 1S0 by from Rangifer antlers. Based on this finding, the base sequences of amplified PCR products in the range of mitochondria) cytochrome b gene from Cervidae antlers and Rangifer antlers were determined and subjected to restriction analysis by various endonucleases. The results showed that antlers from Rangifer species could be simply discriminated with other antlers from 8 Cervidae species (Chinese deer, Russian deer, Hong Kong deer, New Zealand deer, Kazakhstan deer, elk, red deer and Sika deer) by PCR-RFLP analysis using AtuI, HaeIII, HpaII or Sau3AI(MboI) as well as TaqI in the range of the mitochondrial cytochrome b gene.

• Research in Community and Public Health Nursing
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• v.10 no.2
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• pp.320-329
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• 1999

This study has been done for the purpose of testing the effect of Health promotion Education on the Health perception and Health behavior performance of Elementary school student's. The collection data has conducted from June 19, 1999 to August 24, 1999. The subjects for this study were sixth grade of 'ㅅ' elementary school. which is located in 'ㄱ' city a chosen one class experimental group(38) and as a control group(38). The study were designed as nonequivalent control group pretest. posttest. follow test design. In pretest, the general characteristics of two groups, health perception and Health performance were measured, the Experimental group was given health promotion Education for a one week after the posttest, and follow test was done Health performance. for eight week's summer vocation. The instrument used for this study were Health perception scale developed by Ware(l979) were modified by Lee(l984) and Health promoting behavior scale developed by Kim(l997) were modified by No Tae Su(l999). The data analysis was done using t-test, $x^2$ -test, ANOVA. and pearson correlation coefficient using SAS/PC program. The result of this study are summarized as follows: l) There is on difference between experimental group and control group 2) The hypothesis is factor's are supported 'The experimental group which was given health promotion education will shows higher health perception and health behavior performance than control group which given that' (meal habit F=6.40 P<.05. mental health F=8.02 P<.01) 3) In health behavior performance, scale the highest domain was mental health, personal hygiene, meal habit Exercise. The following suggestions are made based on the above results: 1) Replication of the research is needed to confirm effects of health perception and Health promotion education including the elementary school students. 2) Elementary school teachers should make an effort to develop of Health perception progress and carry about continue Health promotion education program for profit stage of growth and development for elementary school students.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.28 no.7
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• pp.528-539
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• 2017

Since IoT is expected to enable hyper-connected society to be realized with the advent of the $4^{th}$ Industrial Revolution, many advanced foreign countries as well as United Kingdom identified IoT spectrum policy as one of the first priority in spectrum management in order to cope with the frequency demands required for the promotion of IoT service. In Korea, the frequencies of 110 MHz bandwidth has been also supplied additionaly for IoT in July 2016 in order to activate a promising new IoT industry based on wireless communication, which has great potential for future industrial growth. Therefore, in this paper, we propose spectrum policy and the research and innovation trends on IoT to promote IoT industry by analyzing the major communication network, the key challenge technologies and the spectrum policy framework of foreign countries.