• Asian Pacific Journal of Cancer Prevention
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• v.16 no.17
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• pp.7943-7957
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• 2015

Background and Aims: Colorectal cancer is one of the leading causes of death in the world. The aim of this study was to investigate the growth-suppression potentiality of a crude saponin extract (CSENS) prepared from medicinal herb, Nigella sativa, on human colon cancer cells, HCT116. Materials and Methods: HCT116 cells were subjected to increasing doses of CSENS for 24, 48 and 72 h, and then harvested and assayed for cell viability by WST-1. Flow cytometry analyses, cell death detection ELISA, fluorescent stains (Hoechst 33342 and acridine orange/ethidium bromide), DNA laddering and comet assays were carried out to confirm the apoptogenic effects of CSENS. Luciferase reporter gene assays, quantitative reverse transcription-polymerase chain reaction and Western blot analyses were performed to assess the impact of CAERS and CFEZO on the expression levels of key regulatory proteins in HCT116 cells. Results: The results demonstrated that CSENS inhibited proliferation and induced apoptosis. Apoptosis was confirmed by flow cytometry analyses, while CSENS-treated cells exhibited morphological hallmarks of apoptosis including cell shrinkage, irregularity in cellular shape, cellular detachment and chromatin condensation. Biochemical signs of apoptosis, such as DNA degradation, were observed by comet assay and gel electrophoresis. The pro-apoptotic effect of CSENS was caspase-3-independent and associated with increase of the Bax/Bcl-2 ratio. CSENS treatment down-regulated transcriptional and DNA-binding activities of NF-${\kappa}B$ and AP-1 proteins, associated with down-regulation of their target oncogenes, c-Myc, cyclin D1 and survivin. On the other hand, CSENS up-regulated transcriptional and DNA-binding activities of Nrf2 and expression of cytoprotective genes. In addition, CSENS modulated the expression levels of ERK1/2 MAPK, p53 and p21. Conclusions: These findings suggest that CSENS may be a valuable agent for treatment of colon cancer.

• The Korean Journal of Community Living Science
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• v.20 no.2
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• pp.263-274
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• 2009

The objective of this study is to verify work satisfaction of school food-service employee, of their job characteristics. Subjects were comprised 9 elementary schools, 11 middle schools, and 3 high schools in Ansan city. Data were analyzed 203 questionnaires for frequency, means, one-way ANOVA, $Scheff{\acute{e}}$ test, t-test, and Pearson correlation using SPSS PC Package. Most of the respondents were high school graduate(91.1%), under one million won salary(41.9%), and non license holder(60.6%). Working conditions were part-time job(41.9%), unlimited contractors(51.2%), and 3-5 years of working experience(21.7%). Measure of overall job satisfaction by Likert-type 5 scale, satisfaction of work operation attitude was 3.55 points and work characteristic duty of 4.32 points. The certificate qualified and elementary school's working posture were more satisfied their work attitude than other groups(p<0.05). Salary satisfaction score showed 2.64 points, but the work esteem satisfaction showed 3.34 points. The factor of working circumstance and potentiality satisfaction was only 2.61 points, but interpersonal connection and communication satisfaction was 3.50 points. Between job satisfactions factors, they were highly correlated with each other. The pride and characteristic duty of the work showed the strongest correlated(p<0.001). The satisfaction score of work operation attitude were significantly correlated with characteristic duty(p<0.001), work esteem, interpersonal relationships and communication(p<0.01). With this results, work satisfaction of food service employees showed a relatively high to perform a job task characteristics and work attitudes. However, the working environment and growth in salaries satisfaction were low. A set of duties must be paid according to the labor intensity. As removing the disturbing factors, the improvement in the quality of the feed will be able to expect substantial effects.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.3
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• pp.3-15
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• 2001

The in situ observations and the seawater analyses were conducted for 3 years from August 1996 to August 1999 in order to elucidate and evaluate seasonal variations of marine water quality and trophic state in Mokpo harbour of Korea. Compared the seasonal seawater qualities of Mokpo harbour with the OECD standards of trophic classification in parameters such as Secchi depth, dissolved inorganic nitrogen, phosphate phosphorus and chlorophyll-a, the trophic level of seawater in Mokpo harbour was evaluated to be in mesotrophic or eutrophic state through all 4 seasons and to be in eutrophic state, particularly In summer. The estimation of pollution index by eutrophication showed the seawater quality of Mokpo harbour to deteriorate and fall under the regular grades through all 4 seasons, although the seawater quality of Mokpo harbour was evaluated to be equivalent to the second or third class of the Korean seawater quality standards in view of COD values. The results of eutrophication index estimation showed the high potentiality of red tide occurrence In Mokpo harbour, particularly in summer or fall. In the light of the average atomic ratio of N/P in seawater, the limiting nutrient factor against the growth of phytoplankton was concluded to be phosphorus rather than nitrogen in Mokpo Harbour.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.21 no.1
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• pp.1-12
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• 1999

The purpose of this study was to evaluate the healing process of the calvarial defect filled with hydroxylapatite(HA) and $TGF-{\beta}$ in Rat. 72 Sprague-Dawly rats were divided into 3 groups, control and two experimental groups. Bony defect were artificially prepared in the calvaria of all 72 rats and followed by implantation of HA (experimental group of 24 rats) and HA+$TGF-{\beta}$(another experimental group of 24 rats) into the defects. Sequential sacrifice was performed at 1, 2, 4, 6, 8, 12 weeks of experiment. Obtained specimen was stained with Hematoxylin and Eosin, Masson's Trichrome and Immunohistochemistry. The results were as follows, 1. Granulation tissue was prominent on control group in 1 and 2 weeks. Bony defects were filled with dense fibrous tissue through the whole experimental period and osteoinduction could not be observed in all groups. 2. Inflammatory cell infiltration was prominent on control group in 1 and 2 weeks and osteoclastic activity was high in HA implanted experimental group at 1 and 2 weeks. 3. Inflammatory cell infiltration was less and maturation of fibrous tissue could be found on HA+$TGF-{\beta}$ implanted experimental group at 1 and 2 weeks. 4. Osteoconduction activity was high in HA+$TGF-{\beta}$ implanted experimental group at 2 and 4 weeks but there was no difference after 6 weeks among 3 groups. 5. In grafted site of HA+$TGF-{\beta}$ implanted group, osteonectin expression was slightly increased from 1 week to 6 weeks. In the host site, it was increased from 1 to 4weeks. 6. In grafted site of HA+$TGF-{\beta}$ implanted group, osteocalcin expression was high at 4 weeks. In the host site, we could find the difference among 3 groups. From above results, the HA with mixture of $TGF-{\beta}$ has the potentiality of promoting bone formation in the bony defect area in the rat.

• Review of Culture and Economy
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• v.19 no.2
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• pp.171-193
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• 2016

China's advertising industry has rapidly increased owing to economic growth and big market size. This paper covers the localization and relative efficiency in the Chinese advertising industry. To analyze localization level and efficiency of advertising industry, this paper uses Location Quotient(LQ) and Data Envelopment Analysis (DEA) respectively. According to the results, the first finding shows that the higher regional development level have the relative efficiencies, the higher regional development level also have regional specialization degree. The second finding indicates that Chinese advertising industries are multipolalized region by region. The last finding reveals that advertising industry of middle and west regions has long term development potentiality owing to high specialization degree with low relative efficiencies.

• International conference on construction engineering and project management
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• 2022.06a
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• pp.967-974
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• 2022

It is no news that Nigeria's infrastructure challenge is enormous. In the global ranking, Nigeria ranked low in quantity and quality of its infrastructural provision which has a great impact on the ease of business transaction. Low investments in transportation have brought about the current infrastructural deficit. Recently, the Nigerian government has made effort to address at least to some extent the infrastructural deficit through Public-Private Partnership, but this has not yielded the desired result. Moreover, the sustainability issues relating to railway projects such as, emissions, noise pollution, ecosystem, and other environmental issues calls for urgent attention. Hence, this necessitated consideration on sustainability appraisal for the Chinese rail project in Nigeria. This study reviews sustainability of railway projects built by the Chinese firm in Nigeria with particular emphasis on the environmental and social impact of these projects. The study further identified issues and challenges in project implementation with a particular focus on civil dialogue and community engagements. A detailed literature search was conducted on railway projects and infrastructure by systematically reviewing selected published articles.The analysis of the selected articles identified sustainability issues and potential for improvement of Chinese railway projects and how they contribute to or inhibit competitiveness in the Nigerian railway market. From the literature searched, some of the projects constructed by Chinese firm revealed that there is economic and social impact of railway projects delivered by the Chinese firm in terms of capacity development and knowledge transfer potentiality. For instance, in the just concluded Lagos-Ibadan railway projects, the study gathered that the project brought about 5000 jobs and local staff were trained by the Chinese company, this will boost man power and local content capability. Also, it will significantly improve Nigeria's infrastructure and boost its economic development. The study suggests that Nigerian government should ensure and provide an enabling environment that is conducive for investment on the continent. Peace, improved security, and decent governance are the best conditions for sustainable transportation growth.

• ALGAE
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• v.28 no.1
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• pp.111-119
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• 2013

Marine microalgae are a promising source of organisms that can be cultured and targeted to isolate the broad spectrum of functional metabolites. In this study, two species of cyanobacteria, Chlorella ovalis Butcher and Nannchloropsis oculata Droop, one species of bacillariophyta, Phaeoductylum tricornutum Bohlin, and one species of Dinophyceae, Amphidinium carterae (Hulburt) were cultured and biomasses used to evaluate the proximate comical compositions. Among the determined proximate chemical compositions of the cultured marine microalgae, the highest content of crude proteins and lipids were exhibited in P. tricornutum and A. carterae, respectively. Solvent-solvent partition chromatography was subjected to fractionate each of the cultured species and separated n-hexane, chloroform, ethyl acetate, and aqueous fractions. Nitric oxide production inhibitory level (%) and cytotoxicity effect on lipo-polysaccharide-induced RAW 264.7 macrophages were performed to determine the anti-inflammatory activity. N. oculata hexane and chloroform fractions showed significantly the strongest anti-inflammatory activity at $6.25{\mu}g\;mL^{-1}$ concentration. The cancer cell growth inhibition (%) was determined on three different cell lines including HL-60 (a human promyelocytic leukemia cell line), A549 (a human lung carcinoma cell line), and B16F10 (a mouse melanoma cell line), respectively. Among the extracts, C. ovalis ethyl acetate and A. carterae chloroform fractions suppressed the growth of HL-60 cells significantly at 25 and $50{\mu}g\;mL^{-1}$ concentrations. Thus, the cultured marine microalgae solvent extracts may have potentiality to isolate pharmacologically active metabolites further using advance chromatographic steps. Hence, the cultured marine microalgae can be described as a good candidate for the future therapeutic uses.

• Journal of the Korean association of regional geographers
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• v.1 no.1
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• pp.23-43
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• 1995

This paper is the first part of a research which looks into the regional development process and the quality of life of Youngnam area, and which suggests a prospect and tasks for the future development of the region. Youngnam region has grown rapidly on the basis of labor-intensive light industries and standardized Fordist lage-scale heavy industries through the industrialization and urbanization of South Korea from the 1960s; but recently it has shown a relatively downward trend. The recent economic stagnation of Youngnam region can be seen as a result of uneven regional development in the national scale, which has brought out the increasing subcontracting relation within the region, the geographically excessive concentration of firms, the lack of growth potentiality of high-tech industries, the weakness of producer service, and the shortage of financial activities for capital flows. In addition, construction of physical and social infrastructures and management of urban central functions could not meet properly the rapid economic and urban growth of the region. Because of these problematics inherent in the economy of Yougnam region, the occupational status of regional dwellers is more or less unstable, and the wage level of employee as a whole in Youngnam region is lower than those of Seoul, although the wage level of labourers in manufacturing is relatively high. Moreover, the quality of life of dwellers in the region has some difficulties in the use of resources and ecological environment as well as the unequal provision of means of living and welfare facilities, even though it has been improved materially.

• Journal of Periodontal and Implant Science
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• v.24 no.2
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• pp.219-237
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• 1994

The ultimate goal of clinical periodontal therapy is to achieve regeneration of a healthy connective tissue reattachment. Conventional therapy including scaling, root planing, gingival curettage, gingivectomy and flap procedures of various types results primarily in repair rather than regeneration of the periodontium. In order for periodontal regeneration to occur, progenitor periodontal ligament cells must migrate to the denuded root surface, attach to it, proliferate and mature into an organized and functional fibrous attachment apparatus. Polypeptide growth factors belong to a class of potent biologic mediators which regulate cell differentiation, proliferation, migration and metabolism. Insulin-like growth factor-I (IGF- I ) of these factors appear to have an important role in periodontal wound healing and bone formation. The purpose of this study is to evaluate the effects of IGF- I on the periodontal ligament cells to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were obtained from periodontal tissue explants culture of the first premolar tooth extracted for the orthodontic treatment. Cells were cultured in Dulbecco's modified Eagle medium(DMEM) with 10% fetal bovine serum. Fourth to seventh passage cells were plated in 24 well tissue culture plates and medium changed to serum-free medium prior to addition of growth factors. Cell proliferation was measured by the incorporation of $[^3H]-thymidine$ into DNA, Protein synthesis was determined by measurement of $[^3H]-proline$ incorporation into collagenase-digestible protein(CDP) and noncollagenous protein(NCP) according to the method of Peterkofsky and Diegelmann (1971), And alkaline phosphatase activity was measured as one parameter of osteoblastic differentiation. The results were as follows : The DNA synthetic activity was increased in a dose-dependent manner with IGF- I except for 0.1ng/ml concentration of IGF- I At the concentration of 10, 100ng/ml, IGF- I significantly increased the DNA synthetic activity(P<0.05) The total protein, collagen and noncollagen synthesis was increased in a dose-dependent manner with IGF- I except for 0.1ng/ml concentration of IGF- I. At the concentration of 1, 10, 100ng/ml, IGF- I significantly increased the total protein, collagen and noncollagen synthesis activity(P<0.95, P<0.001). The % of collagen was not effected according to the concentration of IGF- I. The alkaline phosphatase activity was increased in a dose-, time-dependent manner with IGF- I (10, 100ng/ml). In conclusions, the present study shows that IGF- I has a potentiality to enhance the DNA synthesis of periodontal ligament cells with including the increase of the total protein and collagen synthetic activity. The use of IGF- I to mediate biological stimulation of periodontal ligament cells shows promise for future therapeutic applications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.391-418
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• 1983

Urea and ammonium sulfate without and with sulfur group, respectively, were compared with respect to their nutritive effects on the rice plants, Suweon 264 and Jinheung, under the nursery trial on the protected upland and lowland seedbed, the pot trials with their various combination ratios and with added sodium sulfate to urea and the trials on the paddy fields which have undergone urea and ammonium sulfate application for many years. The rice seedlings fertilized with ammonium sulfate surpassed those with urea in growth performance and nutrients absorption of the rice seedlings at nursery period. Such stimulating effect of ammonium sulfate on the growth and nutrients uptake was more remarkable under the upland nursery than under the lowland one. The pot trial with the various combination ratios of urea and ammonium sulfate revealed that the chlorophyll content in leaf blade increased with the increment of sulfate ratio in the combination and the sole application of urea caused the chlorosis of leaf which was more conspicuous in Suweon 264 than in Jinheung. Fertilized with the same active ingradient amount of nitrogen, the rice plants supplied with ammonium sulfate surpassed those with urea in the chlorophyll content consistently under the nursery, the pot and the paddy field trials. The photosynthesis of flag leaf at heading stage increased with the combination rate of ammonium sulfate in the pot trial. The sulfur applicated as supplementary element of nitrogen in the nursery, the pot and the field trials were observed to be in positive relationship to the nitrogen and potassium content, but to be in negative correlation to the calcium content. The sulfur content in the rice plants was higher at early growth stage and decreased with the advance in growth stage. The nitrogen content also showed a similiar tendency to the sulfur content, and the N/S ratio was higher at early growth stage than at later one. The N/S ratio was negatively correlated with the chlorophyll content. In the field experiment, ammonium sulfate surpassed urea in the number of productive tiller, dry matter production and unhulled rice yields, but much stimulating effect of ammonium sulfate on the grain production was shown to be less effective than that on the straw production. The nitrogen and major nutrients content in the rice straw at harvest were higher in the paddy field with long-term ammonium sulfate application than in that with long-term urea application, suggesting that the former might have greater potentiality in nutrients supply than the latter.