• Food Science and Preservation
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• v.10 no.3
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• pp.417-420
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• 2003

In order to study optimum culture condition of yeast medium added deep seawater, we examed samples with 9 yeast strains. The growth rate were measured for Saccharomyces cerevisiae 10, 11, 12, 901 and RCY and Saccharomyces kluyvery DJ97, Saccharomyces cerevisiae YJK, JK99, CMY-28 etc.. The growth of S. cerevisiae 12 was found most active in the deep seawater(hardness 500). The growth rate of S. cerevisiae 901 on medium containing deep seawater(hardness 1000) was faster than that of the yeast on medium without deep seawater. The use of deep seawater on the growth of Sacch.cerevisiae kluyvery DJ97 revealed maximum growth under the condition of hardness 200 of deep seawater and 10％ of sugar concentration.

• Journal of the Korean Society for Heat Treatment
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• v.31 no.5
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• pp.237-243
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• 2018

In-rich InGaN epilayers were grown on (0001) sapphire substrates by radio-frequency plasma-assisted molecular beam epitaxy (RF-MBE). InGaN epilayers grown at various growth condition were observed by SEM, XRD, and RHEED. When plasma power of nitrogen increased from 290 to 350 W, surface morphology and crystal quality became worse according to more active nitrogen on the surface of InGaN at N-rich growth condition. As In composition was reduced from 89 to 71% by changing the incoming flux of In and Ga, surface morphology and crystal quality became worse. In addition, weak peaks of cubic InGaN phase was observed from InGaN layer with 71% In composition by XRD ${\Phi}$ scan measurement. When growth temperature decreased from 500 to $400^{\circ}C$, RHEED diffraction pattern was changed to be from streaky to spotty which means atomically rough surface, and spotty pattern showed cubic symmetry of InGaN clearly. XRD ${\Phi}$ scan measurement gave clear evidence that more cubic InGaN phase was formed at low growth temperature. All these results indicates that extremely low surface mobility of Ga adatom caused inferior crystal quality and cubic InGaN phase.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1062-1067
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• 2003

In this study, corrosion fatigue characteristics of CF8M and CF8A steel were investigated on the simulated PWR condition(Temp.:$316^{\circ}C$, Pres.: 15:MPa). To make the simulated PWR condition. the special test machine consisted of INSTRON, Autoclave, LOOP and Measurement system was developed. As ${\Delta}K$ is ranged from 11 to $20MPa{\sqrt{m}}$, Crack growth rate of PWR condition is faster than air condition. Above $20MPa{\sqrt{m}}$, the crack growth rate of PWR and air condition is similar. Corrosion fatigue characteristics regardless of the ferrite contents($10{\sim}25wt.%$) is not different. After the test, the fracture surface of specimens was examined. It was difficult to verify the fracture modes such as striation, intergranular crack and cleavage and so on. As the ferrite content of CF8M is increased, the more particles covered fracture surface were peeled.

• Clinical and Experimental Reproductive Medicine
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• v.32 no.2
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• pp.91-99
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• 2005

Objective: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. Methods: Preantral follicles were isolated mechanically from mouse ovaries that were surgically recovered from mice aged 14 days. Retrieved preantral follicles were placed in EG (Ethylene Glycol) for 2, 5, 10 minutes and transferred to EFS-40 (40% EG, 18% Ficoll-70, 0.5 M sucrose) for 0.5, 1, 2 minutes. And then, preantral follicles were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing was carried out at room temperature. After defining the most appropriate vitrification condition that yields high survival, in vitro growth and ovulation rate of follicles were evaluated. Results: Appropriate vitrification condition that yield high survival rate ($83.2{\pm}2.1%$) of preantral follicle was EG for 5 minutes and EFS-40 for 0.5 minutes. In vitro survival rate of the vitrified preantral follicles were $85.5{\pm}0.5%$, $67.9{\pm}0.8%$ and $40.2{\pm}0.5%$ on day 2, 6 and 10. And in vitro growth of the vitrified preantral follicles were $107.1{\pm}16.1{\mu}m$, $117.1{\pm}18.4{\mu}m$, $178.4{\pm}45.6{\mu}m$ and $325.4{\pm}54.4{\mu}m$ on day 0, 2, 6 and 10. Although in vitro survival rate and growth of vitrified preantral follicles were lower than that of non-vitrified preantral follicles, the patterns of survival and growth were similar in vitrified and non-vitrified preantral follicles. The ovulation rate of antral follicles that was grown from vitrified preantral follicles was $32.6{\pm}1.2%$. Conclusion: Vitrified preantral follicles could be grown to antral sizes, and mature oocytes that can be used for IVF-ET programs were produced successfully. These data suggest that cryopreservation of preantral follicle by vitrification can be used for the preservation of the fertility.

• Journal of Korean Society on Water Environment
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• v.25 no.2
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• pp.221-226
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• 2009

We investigated the most effective cutting interval for underwater nutrient removal through cut off the emergent part of hydrophytes at artificial vegetation island (AVR) which was installed for the purpose of water quality improvement in Lake Paldang. We divided the planting area of Phragmites japonica into three parts according to the cutting intervals. The shoot height and relative growth rate of P. japonica, nutrient contents and biomass of cut off P. japonica were measured at each cutting interval. The amount of nutrients which were removed through cut off at each cutting interval was calculated. P. japonica showed full growth, 80% and 60% of full growth before first cut off at three-months, two-months and one-month cutting interval condition respectively. Three-month cutting interval condition showed the largest biomass of cut off P. japonica and one-month cutting interval condition showed the least. However the cut off P. japonica showed the highest content of nutrients at one-month cutting interval condition and the least at three-month cutting interval condition. The amount of phosphorus and nitrogen removal at two-month cutting off condition is the largest among three cutting interval conditions indicating that cut off the emergent part of P. japonica every two months is the most effective to remove the nutrients from water at AVR in eutrophic lakes.

• Asian Journal of Turfgrass Science
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• v.15 no.2
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• pp.65-76
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• 2001

This study was designed to estimate on the interaction of the first productivity, light condition and to examine the ecophysiological characteristics of Zoysia japonica. rtificial community of Zoysia japonica was analyzed effect of matter production and growth construction under various shading condition. The results summarized this experiment were as follows; 1. The relative growth rate (RGR) in all experimental plots was high during the growth stage of 20 days after transplanting, and then decreased. The maxium RGR value appeared in the control plot (2.13g/g. 10days) during the growth stage from 10 to 20 days after transplating. RGR value in server shading of short day condition was remarkably decreased from the early growth stage. The main factor to lower RGR value considered as a short day condition than that of shading. 2. The net assimilation rate (NAR) in all experimental plots except 3hour photoperiod plot was high during the early growth stage after transplanting, and then decreased early growth stage after transplanting, and then decreased as the growth proceeded. The maxium value appeared in the control plot (35g/g. 10days). NAR value in the severe shading of short day condition plots was low. Especially, NAR value in the three hours photoperiod plot was remarkably low from the early growth, there was no great difference by growth stage. NAR had negative correlation with LAI and positive correlation with RGR. 3. The increasing rate of leaf area ratio(LAR) was high during the early growth stage after transplanting in the control plot and in the photoperiod plot, the shorter the exposure time to sunlight, the higher the LAR value from the early growth stage. Especially, its value in the 3 hour photoperiod plot was remarkably high but its increasing rate was lower as the growth proceeded. LAR hade negative correlation on RGR and NAR. 4. The crop growth rate(CGR) in each experimental plot was increased until 50 days after transplanting, and then decreased. After that time, the maxium CGR value appeared in the control plot (1.56g/$\textrm{cm}^2$. 10-days), 60 days after transplanting, CGR had positive correlation with LAI. The optimum LAI in the control, 9 hour, 7 hour, 5 hour, and 3 hour photoperiod plots appeared as 1.87, 1.12, 0.83, and 0.18.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11a
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• pp.29-30
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• 2002

The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1$\^$-1/) or TDZ (1-2 mg1$\^$-1/). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant. The second series of experiments was studied to investigate the effect of physical environment factors on growth of in vitro plantlets. The Anoectochilus formosanus plantlets were cultured under different air exchange rate (0.1, 0.9, 1.2h$\^$-1/), without sucrose or supplement 20g.1$\^$-1/ (photoautotrophic or photomixotrophic, respectively), and different photosynthesis photon flux (40, 80, 120 ,${\mu}$mol.m$^2$.s$\^$-1/- PPF). Under non-enrichment CO$_2$ treatment, slow growth was observed in photoautotrophical condition as compared with photomixotrophical condition on shoot height, fresh weigh and dry weight parameters; High air exchange (1.2.h-l) was found to be inadequate for plant growth in photomixotrophical condition. On the contrary, under CO$_2$, enrichment treatment, the plant growth parameters were sharply (visibly) improved on photoautotrophic treatments, especially on the treatment with air exchange rate of 0.9.h-1. The growth of plant in photoautotrophic condition was not inferior compared with photomixotrophic, and the best growth of plantlet was observed in treatment with low air exchange rate (0.9.h-1). Raising the PPF level from 80 to 120${\mu}$mol.m$\^$-2/.s$\^$-1/ decreased the plant height, particularly at 120${\mu}$mol.m$\^$-2/.s$\^$-1/ in photoautotrophic condition, fresh weight and dry weight declined noticeably. At the PPF of 120${\mu}$mol.m$\^$-2/,s$\^$-1/, chlorophyll contents lowed compared to those grown under low PPF but time courses of net photosynthesis rate was decreased noticeably. Light quality mainly affected morphological variables, changes of light quality also positively affected biomass production via changes in leaf area, stem elongation, chlorophyll content. Plant biomass was reduced when A. formosanus were grown under red LEDs in the absence of blue wavelengths compare to plants grown under supplemental blue light or under fluorescent light. Stem elongation was observed under red and blue light in the present experiment. Smaller leaf area has found under blue light than with other lighting treatments. Chlorophyll degradation was more pronounced in red and blue light compared with white light or red plus blue light which consequent affected the photosynthetic capacity of the plant. The third series of experiment were studied to investigate the effect of physical environment factors on growth of ex vitro plants including photosynthesis photon flux (PPF), light quality, growing substrates, electrical conductivity (EC) and humidity conditions. In the present experiments, response of plant on PPF and light quality was similar in vitro plants under photosynthesis photon flux 40${\mu}$mol.m,$\^$-2/.s$\^$-1/ and white light or blue plus red lights were the best growth. Substrates testing results were indicated cocopeat or peat moss were good substrates for A. formosanus growth under the greenhouse conditions. In case of A. formosanus plants, EC is generally maintained in the range 0.7 to 1.5 dS.m-1 was shown best results in growth of this plant. Keeping high humidity over 70% under low radiation enhanced growth rate and mass production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.160-168
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• 2003

This study was carried out to investigate the effects of induced wet-injury conditions on barley seedling growth. Barley seedlings at the three leaf stage were grown in culture solutions with various combinations of dissolved oxygen (DO), nutrients and citric acid (CA) concentrations. Seedling growth was reduced by hypoxia. Root length and root fresh weight were most severely reduced under 1 ppm DO. Shoot growth was slightly reduced but not root growth under low nutrient condition. Seedling growth was reduced by CA in a concentration-dependant manner, Response of seedling growth was efficiently differentiated in a solution containing 1 ppm DO, one tenth of the normal P, K and Mg concentration and 3 mM CA. Under this induced wet-injury condition, overall seedling growth of 6 cultivars was reduced by 11 to 19% compared to the control condition. The results suggest that the induced wet-injury condition can be used for the screening of resistant genotypes at the seedling stage.

• Journal of Korean Society on Water Environment
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• v.33 no.5
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• pp.538-545
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• 2017

The purpose of this study is to investigate growth response and toxigenicity under various temperature and nutritional conditions, in order to understand the physioecological characteristics of Aphanizomenon flos-aquae, which is a bloom-forming cyanobacterium in the Nakdong River. The strain was inoculated into media under combinations of four temperatures (4, 12, 21, $30^{\circ}C$) and three nutrients (modified CB medium, P-depleted CB medium, N-depleted CB medium) for 28 days. The algae-inhibition tests were performed to assess the potential allelopathic effects of the strains' filtrates on the growth of four algae strains (Microcystis aeruginosa, Aulacoseria ambigua f. spiralis, Aphanizomenon flos-aquae, Scenedesmus obliquus). Toxin production of a strain was measured by Enzyme-Linked ImmunoSolbent Assay (ELISA). The optimal growth temperature (Topt) of strains was $19.9^{\circ}C$ ($18.3-21.2^{\circ}C$), and the temperature range for growth was from $-0.3^{\circ}C$ to $34.3^{\circ}C$. Specific growth rate (${\mu}$) in modified CB medium varied from 0.10 to $0.16day^{-1}$, and the maximum growth rate (${\mu}_{max}$) was $0.17day^{-1}$. Although growth curves under N-existed and N-depleted conditions were almost the same, growth under N-depleted condition was relatively slowed (${\mu}=0.09$ to $0.14day^{-1}$), with a decreased maximum cell density. However, growth under the P-depleted condition was restricted for all temperatures, Two stains of Aphanizomenon flos-aquae were confirmed as not producing toxins, because saxitoxin and cylindrospermopsin were not detected by ELISA. The exudates or filtrates from the Aphanizomenon flos-aquae (DGUC003) resulted in significant inhibition of algal growth on the Aulacoseira ambigua f. spiralis (DGUD001) and Aphanizomenon flos-aquae (DGUC001) (p < 0.01).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.738-746
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• 1995

Greenhouse studies were carried out to find the difference of growth, weed competition and herbicides response in barley(Hordeum vulgare L. emend Larnark) under foggy and non-foggy condition. Plant height, leaf stage, leaf width and shoot fresh weight of barley under foggy condition were greatly increased, while heading rate ripening rate and number of grains per panicle of barley were reduced. Weed emergence based on fresh weight was much greater under foggy than that under non-foggy condition. Plant height of barley under foggy condition was increased comparing with non-foggy condition and significantly reduced with increasing the duration of weed competition, while 1,000-grain weight of barley reduced by the early competition(0∼20 days). Among the herbicides treated, butachlor and thiobencarb inhibited growth of barley under foggy than non-foggy condition. Plant height of barley treated of herbicides under foggy condition was ever increased but 1,000-grain weight of barley was reduced. Weeding efficacy(75-90%) by shoot fresh weight of weeds under foggy condition at 25 days after application was lower 3 to 15% than that under non-foggy condition.