• Applied Biological Chemistry
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• v.43 no.4
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• pp.231-235
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• 2000

The changes of calmodulin levels, calmodulin-binding proteins, and $Ca^{2+}$/calmodulin-dependent glutamate decarboxylase during the growth of tobacco suspension cells were investigated. Tobacco cells exhibited a typical growth curve, including an exponential growth phase between 3 and 5 days after inoculation, and an apparent stationary phase occurring after 5 day. Although slight changes were observed from sample to sample, calmodulin protein levels remained similar during the phases of culture growth. Several $Ca^{2+}-dependent$ calmodulin-binding proteins including 56, 46, 36, and 32-kDa proteins were detected in tobacco cell extracts. The 56-kDa protein was identified as glutamate decarboxylase by Western-blot analysis using an anti-GAD monoclonal antibody. The levels of GAD protein and the specific activity of GAD enzyme were highest during the middle exponential phase of the culture growth cycle. These data suggest that $Ca^{2+}$/calmodulin-dependent glutamate decarboxylase is modulated during the growth of tobacco suspension cells.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.60-65
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• 1989

The Quality characteristics of pH, ginsenosides, fatty acid, phenolic compounds were studied for their changes during growth of Candia parapsilosis. The yeast growth not only scarcely affected the total amount of saponins and ginsenosidvs of recd ginseng tail root extract, but also was not affected by the saponin C. pnrapsilosis did not utilize the ginsenosides as a carbon source. Glucose, fructose and free sugars were utilized in the initial phase of growth, whereas sucrose and maltose were used as the growth continued and completely redured after 43 hours of incubation. Unsaturated fatty acids were significantly reduced with cell growth, showing a relationship between unsaturated fatty acid content and the yeast growth, whereas the amount of saturate[1 tatty acids in red ginseng extract was not affected by the yeast growth. Generally, there were no changes in major organic acids and phenolic compounds (vanillie acid , m-coumaric acid) except the 50% reduction in maltol and ferulic acid in the ginseng extract. The amounts of amlno acids were gradually decreased, but that of arginine was remarkably reduced.

• Journal of Convergence for Information Technology
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• v.8 no.6
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• pp.15-20
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• 2018

This study aimed to find an effective intervention measure through establishing the correlation between self-regulation (control over life style) and height growth through neuro-feedback training. 40 elementary students in grades two to four with height growth programs (20 experimental group students, 20 control group students) were examined for the changes before and after undergoing neuro-feedback training. The experiment lasted for three months with one 30-minute training session two times a week. After analyzing the differences in self-regulation among the control group with no neuro-feedback training and the experimental group with neuro-feedback training, the differences in height growth were analyzed. First of all, there were positive changes in self-regulation of the experimental group compared with the control group. Secondly, the experimental group showed larger changes in height growth. In conclusion, neuro-feedback training had positive effects upon the self-regulation that adjusts the acquired factors of height growth, which led to positive effects.

• Applied Microscopy
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• v.8 no.1
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• pp.1-8
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• 1978

The aim of this research was to discover the action mechanism of various antituberculosis agents (isoniazid, paraaminosalicylic acid and streptomycin) which act on Mycobacteria tuberculosis hominis $H_{37}R_v$ and also to study the relationship of ultrastructural changes and the growth pattern in Mycobacterium tuberculosis hominis. The ultrastructural change was observed with an electron microscope while the growth pattern was studied through in vitro culture. The results are summarized as follows: 1. The ultrastructural changes found in the group treated only with isoniazid were the loss of nuclear materials and the appearence of electron dense granules. 2. In the group treated with paraaminosalicylic acid, thickening of nuclear filaments and meso some arrangement disorders were observed. 3. In the group treated with streptomycin, the ribosome particles appeared indistinct and the cytoplasm was denaturalized. 4. In the group cross treated with all three agents, all the ultrastructural changes mentioned above could be observed in the cell just as they appeared in the single treated groups. 5. In all of the single and in the crossly treated group, there were no significant changes note in the cell wall or cytoplasmic membranes of any of the cells observed. 6. In the cultural data in vitro, through the crossly treated group and single treated group. growth was observed in 3-5 weeks of culture.

• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.141-151
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• 1992

Physiological changes of the murine hybridoma cell line $S3H5/\gamma{2bA2}$ during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.

• Korean Journal of Poultry Science
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• v.22 no.4
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• pp.203-211
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• 1995

In order to investigate the effect of feeding live yeast on growth performance, nutrients utilization, tibia mineral deposit and intestinal microorganism changes, a growth assay was conducted with 360 broiler chicks. Treatments were four levels of yeast as 0, 0.025, 0.05 and 0.1% in 1.83% tricalcium phosphate and two levels of yeast as 0 and 0.05% in 1.15% tricalcium phosphate. The crude protein content of live yeast was 45%, and 97% of it was in the pure protein form, with 46.6% of essential amino acids and 53.4% of non-essential amino acids. Growth performance was tended to increase by feeding the yeast but there was no significant difference(P>.05). The protein digestibility was increased as the feeding level of yeast increased. However, digestibilities of fat, fiber, calcium and phosphorus were not affected by the yeast. Ash and calcium content of tibia were increased as the level of yeast increased. Total number of E. coli in small intestine was significantly decreased(P<.05) in chicks fed yeast. Total number of Lactobaci1lus was significantly increased by the yeast feeding. The changes of microorganism in cecum had the same trend with the changes of microorganism in small intestine.

• Korean Journal of Organic Agriculture
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• v.18 no.2
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• pp.135-154
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• 2010

The green craze has hit all the world lately. The green revolution which means immense changes for sustainable development under the new environment-economy system is foretelling that we should have new angle to the environment and economy. This paper is about the development of the model and indicators for construction of ECO(Energy Circulating Organic)-Village which is the green growth option of local community. This model is composed of three concepts - SPDC (about procedural methodology of Model), Virtuous Circle (about functional relationships of three requisites), Network (about spatial relationships between communities) - and evaluation indicators. Environment, tourism, industry (Energy & Organic Agriculture) are set as the three requisites for green growth of local community. Nations of the world plunge into the green war which is silent, general, sweeping changes. We can not win this war with the existing thinking system and policy. We need revolutionary changes reconsidering of environmental value and sustainable development strategy of the region. Each of us should go through the changes and this work would be the one among those efforts.

• Korean Journal of Remote Sensing
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• v.11 no.2
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• pp.59-73
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• 1995

The study is focused on the analysis of geomorphological environment changes of alluvial bar in lower Kum river using satellite-based multitemporal/multisensor data. Landsat datas for environment changes analysis consists of Landset MSS(2 scenes) and Landset TM(7 scenes) acquired from 1979 to 1994. This study is to develop the analysis techniques for the environment change detection of using ratio, classification, false color composite etc, of Landsat data especially useful to the geomorphological study of tidal flats and river channels. The results of this study can be summarized as follows : 1. The lower Kum River alluvial bar have had rapid geomorphological changes after the construction of the temporary dam to block the river flowing in 1983. The most alluvial bar located in the river has both bankway growth, especially the allurival bar in the Lower Kum River had grown between 1983 to 1990. 2. After construction of the estuarine barrage, no remarkable geomorphological changes have been found in Kum River area but the growth and formation of new underwater bar has continued. The enormous materials was needed for the growth and formations of new underwater barrier oslands and bar would be supplied from the sea bottom and river sediment to diminish of stream velocity after construction of the estuarine barrage.

• STI Policy Review
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• v.2 no.2
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• pp.11-24
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• 2011

S&T policy has been traditionally regarded as a sector policy; however, it is now evolving into an infrastructure innovation policy that forms the foundation of diverse types of policies. Simultaneously, environmental and energy policies formerly considered as sector policies are now being expanded and integrated into a higher level policy for sustainable development. With these changes underway, the importance of policy integration has increased. Efforts are being made to minimize contradictions between environmental, social, and innovation policies that emphasize proactive linkage among policies or place the highest priority on environmental policy following the theory of Environmental Policy Integration (EPI). Confronted with these policy changes, the Lee Myung-bak government announced "Low-Carbon Green Growth" in 2008 as national agenda for development that focus attention on environmental and energy issues. Economic policy and environmental policy have been traditionally seen in a conflicting relationship with different paths of policy development. However, the administration of President Lee is now emphasizing the synergy effects between the environment and economic growth with the concept of green growth. The green growth policy of the Korean government has great significance as it has built a momentum for incorporating social goals such as environmental values or sustainable development into economic growth-oriented policies; however, there remain many challenges due to the legacy of the development period that has dominated Korean society. The Korean government says it reflects "EPI" or "environmentalism" in policy goals; however, in reality it prioritizes development over the environment.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.187-193
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• 1998

Seasonal changes of nitrogen fixation and growth characteristics of Melilotus suaveolens population under different light conditions were quantitatively analyzed during growting period. Height growth of M. suaveolens population was promoted after April and the shade (site 2) showed 10-20% increase of height growth in compared with that of sunny (site 1). Seasonal changes of biomass were in creased after March, the biomass at site 1 attained 78.5% that of site 2 in May. Chlorophyll content was maintained high value from March to April but, Chlorophyll content of leaf at site 1 was decreased 11.7% compared to that of site 2 in May. The nitrogen content of each organ of M. suaveolens showed higher value in March and then decreased, the high nitrogen contents of reproductive organ were achieved at the flowering stage at site 1. The root nodule of M. suaveolens population appeared in March and showed maximum value in early June and decreased remarkably after July, the amount of nodule formation of M. suaveolens at site 2 showed higher than that of site 1. The nitrogen fixation activity of root nodule of M. suaveolens initiated with shoot growth and showed maximum value in mid April, and attained second peak in early June and then decreased after July. The total nitrogen fixation of the plant showed higher value at site 2 than that of site 1. From the overall results, M. suaveolens showed increased growth under half-shady, well ventilated barren soil than fertile sunny site.