• 한국수산과학회지
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• 제48권5호
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• pp.704-711
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• 2015

We examined the effects of temperature and light intensity on gametophyte growth of Ecklonia cava Kjellman. The growth of female and male gametophytes was investigated before maturation. Gametophytes prepared from Munseom, Jeju, Korea in October 2014 were separated by sex and maintained under the following conditions: 15℃, 20 μmol·m^-2·s^-1, and a 12-h/12-h light/dark cycle. The experiments were conducted at five temperatures (10, 15, 20, 25, and 30℃) and three light intensities (5, 20, and 40 μmol·m^-2·s^-1). Daily growth rates were estimated by the increase in filamentous frond area. The optimal growth conditions for female gametophytes were 20℃ and 40 μmol·m^-2·s^-1; the optimal conditions for male gametophytes were 15℃ and 20 μmol·m^-2·s^-1. The relative growth rates (RGR) of female and male gametophytes decreased at 25℃ and 30℃, respectively. At 30℃, RGRs of gametophytes decreased by approximately 1.0% day^-1. In addition, RGRs of male gametophytes were higher than those of female gametophytes. These results indicate that female gametophytes were more sensitive to temperature and light intensity than male gametophytes. Moreover, these results suggest that E. cava growth rates could decrease as a result of global warming.

• 한국가축번식학회지
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• 제25권2호
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• pp.101-111
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• 2001

본 실험은 preantral 난포를 antral 단계까지 성숙시 비교, 또 난포 성숙, 수정 및 배발달을 유도할 수 있는 체외배양체계를 개발하고자 시행되었다. 협막세포로 둘러쌓인 소 preantral난포 (150 $\pm$ 1.2 $\mu\textrm{m}$)를 1 mg/$m\ell$ collagenase와 0.2 mg/$m\ell$ DNase I가 포함된 Leibovitz L-15 배양액에서 효소적 방법과 물리적 방법으로 난소조직으로부터 분리하였고, 체외난포 성숙 배양액인 $\alpha$MEM을 기본 배양액으로 여러 농도의 FSH와 LH를 첨가하여 25일간 배양하였다. FSH (10~150 ng/$m\ell$) 첨가군의 성숙율과 생존율은 대조군의 그것보다 유의하게 높았으나(P ＜ 0.001), LH (1~125 ng/$m\ell$) 첨가군들과 대조군 사이에서는 유의차가 없었다. FSH (90 ng/$m\ell$)와 LH (25 ng/$m\ell$)를 공동첨가군의 생존율 (40%)과 성숙율 (244$\pm$0.5 $\mu\textrm{m}$)은 대조군의 그것 (25%, 160 $\pm$0.5 $\mu\textrm{m}$)보다 양호하였다. 결과적으로, preantral 난포를 25일간 체외배양한 후, 50%의 건강한 antral 난포를 획득할 수 있었고, 이들 난포중 60%가 제 1극체가 있는 완전한 감수분열을 이루었고 (18.1%), 10.0%가 배발달을 하여 배반포 단계에 이르렀다. 이런 결과는 협막세포가 있는 소 preantral 난포는 상기의 체외배양체계에 의해 antral 단계까지 성장할 수 있고, 체외에서 성숙된 소 preantral 난포의 난자는 분열능을 획득하고 수정 및 배발달이 가능하다는 것을 보여주었다.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.41-41
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• 2003

Transgenic triploid hybrid between fast-growingtransgenic mud loach (Misgurnus mizolepis) males and cyprinid loach (M. anguillicaudatus) females were generated and their performance on growth, feed conversion ability and reproduction were evaluated. Although the growth accelerations of diploid and triploid transgenic hybrids were not as much as those of original transgenic mud loaches, they still represented persistent growth stimulation ranging 11 to 28 fold when compared to their non-transgenic counterparts, with significantly improved feed conversion efficiency up to 2-fold (compared to non-transgenic hybrid) and 1.5-fold (compared to non-transgenic mud loach) in maximum. The gonad development of diploid hybrids was fertile in histological views regardless of transgenic genotypes but the extent of developmentin hybrid fish were less than mud loach diploids at the same age. On the other hands, very stringent sterility was obtained in both sexes of the triploid hybrid transgenics: ovary and testis from transgenic triploid hybrids were significantly depressed and any notable sign for maturation to ovum or spermatids was not detected. No viable embryo was obtained in a fertilization trial using the suspension prepared from the minced testes of transgenic triploid hybrids. This study may indicate the potential usefulness of triploid hybridization as a mean for reproductive containment of transgenic mud loach.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2000년도 국제심포지움
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• pp.9-9
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• 2000

The mammalian ovary has a large number of primordial and preantral follicles, which are a potential source of oocytes for the in vitro mass production of embryos. Several in vitro culture systems have been developed to support the growth and development of oocytes from mouse preantral follicles. Under the appropriate condition, meiotically incompetent oocytes from preantral follicles can grow to final size and complete nuclear maturation in vitro. Furthermore, the successful production of live young from in vitro grown and matured oocytes demonstrates that oocytes from preantral follicles are able to acquire full developmental capacity in vitro. However, the efficiency of in vitro production of embryos from mouse preantral follicles is still low. In farm animals as well as human, the growth of oocyte from preantral follicle to the meiotic competence stage has yet to be demonstrate. Therefore, further studies to improve the culture condition or to develope new culture system should be needed in the future. In addition, the visible progress in the establishment of the in vitro culture system for preantral follicles of farm animals and human could help to enlarge the populations of valuable agricultural, phamaceutical product-producing, and endangered animals, and to rescue the oocytes of women about to undergo clinical procedures that jeopardize oocytes.

• 한국잠사곤충학회지
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• 제13권2호
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• pp.135-139
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• 1971

1. Ecdysterone의 숙화 촉진작용은 5령 성식기이후에 유효하다. 2. Ecdysterone은 누에의 숙화를 촉진하고 또 초숙잠과 종숙잠간의 시차폭을 축소시킨다. 3. Ecdysterone의 숙화 촉진작용은 Ecdysterone의 식하량과 비례한다. 4. Dodecyl alcohol 주제의 DAT는 등족작용을 촉진한다. 5. Ecdysterone과 Dodecyl alcohol의 병용은 숙화와 등족을 촉진하여 자연상족을 가능케 하며 상족기술 개량에 공헌할 줄 믿는다.

• 한국약용작물학회지
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• 제12권5호
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• pp.384-390
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• 2004

An in vtro nucellar polyembryo propagation method was established with mature seed of the Citrus junos Sieb. 7-8 nucellar polyembryos per seed were induced on MS basal medium without plant growth regulators. The polyembryos developed to complete plantlets on teatment with IBA. These shoots grew further in MS medium without plant growth regulators. Rooting of shoots occurred on MS medium supplemented with IBA. These plantlets were successfully transplanted to small plastic pot containing soil mixture. Somatic embryos were induced from nucellar polyembryo and maturation occurred spontaneously from proliferating cultures on MS medium without growth regulators. Random Amplified Polymorphic DNA (RAPD) marker analysis of in vitro and in vivo grown junos orange showed identical polymorphism indicative of their genetic stability. The RAPD polymorphism produced revealed same banding pattern in each regenerant. Hence, propagaton of junos orange by nucellalr polyembryos was efficient and produced in genetically stable plants under in vitro conditions.

• Reproductive and Developmental Biology
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• 제35권4호
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• pp.519-525
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• 2011

Growth hormone (GH) is obligatory for growth and development. But, there is controversy on the GH effect about reproductive processes of sexual differentiation, pubertal maturation, gonadal steroidogenesis, gametogenesis and ovulation. This study was conducted to investigate the effect of GH on estrus, ovulation and embryo implantation. The results obtained were as follows. GH stimulated to increase estrus rate (p<0.05), pregnancy rate (p<0.05), and total fetus number in mice treated for superovulation. Also, the correlation between GH and steroids, E2 and P4, at peri-estrus stage/ peri-ovulation stage/ peri-implantation stage of the superovulation-induced mice was examined. Consequently, GH co-injected with PMSG especially increased P4 level (p<0.05) at peri-estrus stage of superovulationinduced mice. In conclusion, GH co-treatment in superovulation system boosted the rate of estrus, pregnancy and total fetus by increasing progesterone level at peri-estrus stage of superovulation-induced mice.

• 한국식생활문화학회지
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• 제18권6호
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• pp.592-600
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• 2003

Male rats were fed a purified diet containing one of 3 experimental diets, gelatinized rice starch that was not modified physically (RC), gelatinized physically modified rice starch using ultrasonic homogenizer(RU), gelatinized physically modified rice starch using hydroshear homogenizer(RH) during 28 days. RC was used as the rice starch control. Feeding a physically modified rice starch (RU) caused an increase in liver weight and RH increased RNA and protein contents in kidney significantly although there were no differences in food intakes compared to feeding a RC diet. The wet weight of liver, kidney and heart were higher in RU. The wet weights of fecal output of the rats fed RH was greater than in rice control group. The gut transit time was longer in the rats fed RH than in the rice control group significantly. Serum GOT, GPT, total bilirubin concentration were tended to be lower and blood urea nitrogen was significantly lower in RH group. The maturation index of kidney was higher in RU than in RC. These results suggest that physically modified rice starch improved growth performance and physiological functions in organs of growing rats.

• 한국발생생물학회지:발생과생식
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• 제25권1호
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• pp.67-72
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• 2021

This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.469-475
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• 2013

In mammals, puberty is a process of acquiring reproductive competence, triggering by activation of hypothalamic kisspeptin (KiSS)-gonadotropin releasing hormone (GnRH) neuronal circuit. During peripubertal period, not only the external genitalia but the internal reproductive organs have to be matured in response to the hormonal signals from hypothalamic-pituitary-gonadal (H-P-G) axis. In the present study, we evaluated the maturation of male rat accessory sex organs during the peripubertal period using tissue weight measurement, histological analysis and RT-PCR assay. Male rats were sacrificed at 25, 30, 35, 40, 45, 50, and 70 postnatal days (PND). The rat accessory sex organs exhibited differential growth patterns compared to those of non-reproductive organs. The growth rate of the accessory sex organs were much higher than the those of non-reproductive organs. Also, the growth spurts occurred differentially even among the accessory sex organs; the order of prepubertal organ growth spurts is testis = epididymis > seminal vesicle = prostate. Histological study revealed that the presence of sperms in seminiferous tubules and epididymal ducts at day 50, indicating the puberty onset. The number of duct and the volume of duct in epididymis and prostate were inversely correlated during the experimental period. Our RT-PCR revealed that the levels of hypothalamic GnRH transcript were increased significantly on PND 40, suggesting the activation of hypothalamic GnRH pulse-generator before puberty onset. Studies on the peripubertal male accessory sex organs will provide useful references on the growth regulation mechanism which is differentially regulated during the period in androgen-sensitive organs. The detailed references will render easier development of endocrine disruption assay.