• Reproductive and Developmental Biology
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• v.32 no.3
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• pp.183-191
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• 2008

This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax-$\alpha$ and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.

• Korean Journal of Acupuncture
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• v.28 no.4
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• pp.17-28
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• 2011

Objectives : Burn is the important adverse effect of the moxibustion treatment. This research does with the purpose to utilize as basic data which the results of histological observation of the burn tissue which generates when operating various commercial indirect moxa (CIM) on the skin, evaluate the burn because of the surgical operation. Methods : It operated 6 kinds of CIM on the depilated abdomen of the rat. By using the H-E staining and TUNEL assay, the image was observed and analyzed. Results : It could be the moxibustion medical treatment using the CIM generated in the first degree burn until the third degree. And in some case, burn has not generated. By using the H-E stain, the second-degree burn and third degree was observed, and it was observed the first degree burn by using TUNEL assay. In the first degree burn, TUNEL reaction in the epidermal layer was confirmed. The damage to the dermal layer was observed in more than 2 degree burn. In the third degree, the tissue degeneration to the subcutaneous fat was observed but the thickness of the skin tissue was not observed. Conclusions : Basic data classifying the burn generated by the CIM treatment through the histological observation of the burn tissue caused by the CIM treatment were built according to this research result.

• Transactions of Materials Processing
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• v.27 no.1
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• pp.60-65
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• 2018

• ETRI Journal
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• v.26 no.2
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• pp.71-82
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• 2004

We introduce a high-definition three-dimensional television (3DTV) broadcasting system that is fully compatible with the existing transmission system of high-definition television (HDTV). Specifically, we developed high-definition 3DTV broadcasting subsystems including a 3DTV camera, 3DTV video multiplexer and demultiplexer, 3DTV receiver, and 3DTV outdoor broadcast van. To verify the developed subsystems, we performed experimental services of 3DTV broadcasting during the 2002 FIFA World Cup Korea/Japan. According to our subjective evaluation test, 88% of 273 viewers rated the perceived depth of 3DTV as “Good,” and 36% of the viewers preferred 3DTV to other digital broadcasting services.

• Proceedings of the Korea Information Processing Society Conference
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• 2004.11a
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• pp.191-194
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• 2004

차세대 인터넷 서버는 인터넷을 통하여 여러 사용자에게 HDTV 급 고품질 멀티미디어 서비스를 제공하고자 개발된 시스템이며, 이를 위하여 디스크 연결 및 네트워크 기능을 정합 시킨 특화된 NS 카드를 개발하였다. Contents Container 파일 시스템은 NS 카드 전용으로 개발된 멀티미디어 파일 시스템이며, 본 논문에서는 Contents Container 파일 시스템에 대한 개발 내용을 기술하고, 개발 시스템의 장단점을 파악하고자 EXT3 파일 시스템과 비교 분석하였다.

• Natural Product Sciences
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• v.24 no.4
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• pp.284-287
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• 2018

A new isoprenylated acetophenone, acronyculatin P (1) as well as two known compounds, 3',5'-diisoprenyl-2',4'-dihydroxy-6'-methoxyphenylethanone (2) and 3'-isoprenyl-2',4',6'-trihydroxyphenylethanone (3) were isolated from the stem bark of Acronychia pedunculata (L.) Miq. The structures were determined by HRESIMS, 1D and 2D NMR. The inhibitory activity of the isoprenylated acetophenone derivatives against murine leukemia P-388 cells showed compound 1 moderate activity with $IC_{50}$ $15.42{\mu}M$.

• Natural Product Sciences
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• v.28 no.2
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• pp.58-61
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• 2022

Three isoprenylated flavanones were isolated from the leaves of Flemingia lineata (L.) Aiton. Among them are two new flavanones, flemilineatins A and B (1-2), along with 6-isoprenyl eridioctyol (3). Their structures were determined using HRESIMS data and NMR spectra. Flavanones 1 - 3 were assayed in the HeLa cancer cells. Compound 1 showed moderate activity with an IC₅₀ value of 11.2 μM.

• Natural Product Sciences
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• v.28 no.1
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• pp.40-43
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• 2022

Three isoprenylated flavanones were isolated from the leaves of Flemingia lineata (L.) Aiton. Among them are two new flavanones, flemilineatins A and B (1-2), along with 6-isoprenyl eridioctyol (3). Their structures were determined using HRESIMS data and NMR spectra. Flavanones 1-3 were assayed in the HeLa cancer cells. Compound 1 showed moderate activity with an IC₅₀ value of 11.2 μM.

• Proceedings of the KIEE Conference
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• 1996.07a
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• pp.559-563
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• 1996

Total 215 magnet power supplies are under operation in Pohang Light Source(PLS). Current, voltage and peak power handling capabilities of DC power supply are in the range of 21 to 643V, 45 to 850A and 0.94 to 531KW, respectively. The DC power supplies can be categoriezed as uni-polar and bipolar power. Typical required stability and ripple of uni-polar power supplies are ${\pm}0.005%$ and ${\pm}0.05%$, respectively. The bipolar power supplies should be maintained within ${\pm}0.05%$ stability and ${\pm}0.5%$ ripple. Precise measurement results show that all power supplies meet or exceed the required specifications. The long term operation reliability to be sufficient for a stable operation of the Pohang Light Source.

• Carbon letters
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• v.14 no.2
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• pp.89-93
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• 2013

Size-sorted graphene nanoplatelets are highly desired for fundamental research and technological applications of graphene. Here we show a facile approach for fabricating size-sorted graphene oxide (GO) nanoplatelets by a simple centrifugal method using different dispersion solvents. We found that the small-sized GO nanoplatelets were more effectively separated when dispersed in water or dimethylformamide (DMF) than in an alkali aqueous solution. After several iterations of the centrifugation, the sizes of GO in the supernatant solution were mostly several micrometers. We found that the GO area was not strongly correlated with the C-O content of the GO dispersed in water. However, the size-sorted GO nanoplatelets in DMF showed different C-O content, since DMF can reduce GO nanoplatelets during exfoliation and centrifugation processes.