• Journal of Food Hygiene and Safety
• /
• v.29 no.2
• /
• pp.158-163
• /
• 2014

In this study, two commercial PCR and ELISA test kits were examined for identification of eight animal species (beef, pork, chicken, duck, turkey, goat, lamb, and horse) from raw meat and meat products in Korea. The detection limit in RAW meat ELISA kit$^{(R)}$ on three types of meat samples blended with beef, pork and chicken, demonstrated that all meat species were differentiable down to 0.2%. RAW meat ELISA kit$^{(R)}$ on animal species resulted in differentiation rate of 94.5% for beef, 93.3% for pork, 90% for lamb, and 100% for chicken, duck, turkey, goat, and horse. In contrast, Powercheck Animal Species ID PCR kit$^{TM}$ resulted in 100% specificity at 0.05% limit of detection for all meat species. The detection limit of Cooked Meat ELISA kit$^{(R)}$ on mixed meat samples heat-treated with different temperatures and times, resulted in 0.1% for all heat-treated mixed meat except for chicken at 1.0%. Additionally, ELISA kit on sixty meat products resulted in specificity of 31.8% for ham, 13.6% for sausages, and 12.5% for ground processed products, and relatively low rate for more than 2 types of mixed meats. On the contrary, meat species differentiation using PCR kit showed higher percentage than that using ELISA kit$^{(R)}$: 50.0% for ham, 41.7% for sausages, and 28.6% for ground processed meat. Futhermore, PCR kit on 54 dried beef meats detected pork genes in 13 products whereas ELISA kit showed negative results for all products. Hence, the possibility of cross-contamination during manufacturing process was investigated, and it was found that identical tumblers, straining trays, cutters and dryers were used in both beef and pork jerky production line, suggesting the inclusion of pork genes in beef products due to cross-contamination. In this study, PCR and ELISA test kits were found to be excellent methods for meat species differentiation in raw meat and heat-processed mixed meat. However, lower differentiation rate demonstrated in case of meat processed products raised the possibility of inclusion of other species due to cross-contamination during manufacturing process.

• Journal of Radiation Protection and Research
• /
• v.24 no.4
• /
• pp.205-213
• /
• 1999

Effective dose conversion coefficients from unit activity radionuclides contaminated on the ground surface were calculated by using MCNP4A rode and male/female anthropomorphic phantoms. The simulation calculations were made for 19 energy points in the range of 40 keV to 10 MeV. The effective doses E resulting from unit source intensity for different energy were compared to the effective dose equivalent $H_E$ of previous studies. Our E values are lower by 30% at low energy than the $H_E$ values given in the Federal Guidance Report of USEPA. The effective dose response functions derived by polynomial fitting of the energy-effective dose relationship are as follows: $f({\varepsilon})[fSv\;m^2]=\;0.0634\;+\;0.727{\varepsilon}-0.0520{\varepsilon}^2+0.00247{\varepsilon}^3,\;where\;{\varepsilon}$ is the gamma energy in MeV. Using the response function and the radionuclide decay data given in ICRP 38, the effective dose conversion coefficients for unit activity contamination on the ground surface were calculated with addition of the skin dose contribution of beta particles determined by use of the DOSEFACTOR code. The conversion coefficients for 90 important radionuclides were evaluated and tabulated. Comparison with the existing data showed that a significant underestimates could be resulted when the old conversion coefficients were used, especially for the nuclides emitting low energy photons or high energy beta particles.

• Analytical Science and Technology
• /
• v.12 no.4
• /
• pp.284-289
• /
• 1999

Dissolved inorganic carbonates in ground water were quantitatively recovered by using gas evolution method. Gas evolution method was found to be less time-consuming, less susceptible to the contamination fromatmospheric $CO_2$ and little affected by the sulfate ions in comparison to the direct precipitation method. Extraction efficiency of nearly 99% could be achieved by gas evolution method in two hours using recirculated gas at a sweeping rate of 4 liter per minute. Samples for carbon isotope fractionation study were collected in three fractions with collection times. The evolution time for the first fraction was one hours, and then second and third fractions were collected at intervals of 30 minutes, respectively. A small portion of each fraction was analyzed to evaluate ${\delta}^{13}C$ values, which were measured to be -7.9‰, -3.0‰ and +0,4‰ for the each fraction. The result clearly indicated that gas evolution method generates isotopically lighter carbon at the beginning of the purging process and heavier isotopes at the end. However, this isotope fractionation effect could be neglected by the almost complete recovery of carbonates.

• Journal of the Korean association of regional geographers
• /
• v.13 no.6
• /
• pp.609-622
• /
• 2007

This paper examines the lava tubes designated as a natural monument in Jeju Island, and then makes the several suggestions for conserving and managing them. The cave entrances should be scientifically and strictly managed to prevent the deterioration, and to preserve the interior ecosystem, of the lava tubes. The land use and vegetation of the ground surface over the lava tubes should be also systematically managed; the destroyed sand dunes must be recovered for the development of speleothem in the lava tubes, and the plants with deep roots must be removed for the prevention of ceiling collapse. The provincial authorities should manage the ground surface over the lava tubes as a public land to remove the factors threatening the stability of the lava tubes. Since the roads crossing the ground surface are likely to cause the collapse of the lava tubes, new roads bypassing the caves need to be constructed, and warning signs should be also installed at the traverse points. A monitoring system on the cultural properties protection area over the lava tubes could be effectively established by encouraging residents to participate the conservation program of lava tubes. Since the falling blocks from ceilings have been usually observed in the lava tubes, it is urgent to examine their stability. In particular, the dangerous sections must be continuously monitored for taking a quick measure against the collapse. The air environments of lava tubes need to be scientifically analyzed based upon the long-term observations. The lighting equipments must be improved to minimize the green pollution in the lava tubes open to the public. The numbers of visitors should be also controlled to prevent the destruction and contamination of lava tubes caused by a large number of visitors.

• Tunnel and Underground Space
• /
• v.31 no.6
• /
• pp.623-646
• /
• 2021

As the demand for electric power increases with acceleration of electrification at home and abroad, the needs for coal-fired electrical power plant are accordingly increased. However, these coal-fired electrical power plants induce also many environmental problems such as increase of air pollutants, increase of possibility of land contamination by reclamation of coal ash, even though these power plants have a good economical efficiency. In case of a by-product of coal-fired electrical power plants, only 70% of them are recycled and the remaining 30% of by-product are fully buried in surrounding ground. Consequently, this study deals with coal ash backfilling mechanism in abandoned mine openings for the purposes of increasing the coal ash recycling rate as well as securing the mine area stability. In order to analyze the backfill and ground reinforcement by interaction between rock mass and backfills, the copying samples of discontinuous surface with different roughnesses were produced for bond strength tests and direct shear tests. And statistical analysis was also conducted to decide the characteristics of bond and shear behavior with joint roughness and their curing day. Numerical simulations were also analyzed for examining the effect of interface behavior on ground stability.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2001.04a
• /
• pp.98-103
• /
• 2001

In order to understand the characteristics of leachate at the Seokdae municipal waste landfill in the Pusan city, the correlation between leachate pollution loading and volume of gas production. concentration of gas and subsidence of ground, the characteristical methos, geochemical analyses and laboratory column tests using samples of gases, leachate and surface soil of Seokdae waste landfill area. Through the analysis of water balance, leachate flow rate and pollution loading were estimated. Geistatistical analysis of four gas components ( $O_2$, C $H_4$, $H_2$S and CO) shows the possibility of ground subsidence around the group of a site with high concentration of gas. From geochemical analyses of leachate, EC and Total-Alkalinity of ground subsidence around the group of a site with high concentration of gas. From geochemical analysis of leachate, Ec and Total-Alkalinity were increased, and Cl, Cr, Mn, Cu, Zn, Cd and Pb were decreassed comparing to the part, and the type of water quality was Na-HC $O_3$ in trilinear diagram. It shows that biodecomposition of municipal wastes continues actively. From the analysis of water balance, the total leachate flow rate is about 465.11㎥/day and pure pollution loading of Cl, Mn and Fe are estimated to 223.8kg/day, 0.2kg/day, 0.3kg/day, respectively. The laboratory column test of residual soil and landfill soil shows 0.206cm and 0.019cm for linear velocity(equation omitted), 0.234 $\textrm{cm}^2$/min and 0.018$\textrm{cm}^2$/min for diffusion coefficient ( $D_{ι}$), and 1.136cm and 0.095cm longitudinal dispersion index ($\alpha$$_{ι}$), respective]y. It demonstrates that the delay time of contamination for residual soil is shorter than that of landfill soil.

• Journal of Korean Society of Environmental Engineers
• /
• v.33 no.10
• /
• pp.756-766
• /
• 2011

In order to investigate the degree of apartment parking lot dust contamination, total 72 samples of parking lot dust (36 from ground parking lots and 36 from the underground parking lots) were collected in Daegu city from the end of March to the early June 2010. The dust samples were sieved below $100{\mu}m$, and analysed by ICP for 14 elements after an acid extraction. Results obtained from the source assessment of trace element using enrichment factor showed that Fe, K, Mg, Mn, Na and V were influenced by natural sources, while Cd, Cr, Cu, Ni, Pb and Zn were influenced by anthropogenic sources in both the ground parking lot and the underground parking lot. And results showed that Ca were influenced by natural sources in the ground parking lot, but influenced by anthropogenic sources in the underground parking lot. The measured values were remarkably higher in components from natural sources than in components from anthropogenic sources. Underground parking lot dust was more affected by anthropogenic sources and contaminated compared with the ground parking lot dust. Pollution index of heavy metals revealed that underground parking lot dust was 5.5 times more contaminated with heavy metal components than the ground parking lot dust. The results of correlation analysis among trace elements indicated that components in the ground parking lot were more correlated than those in the underground parking lot, and especially more correlated with natural sources-natural sources. Analysis for correlations between components and influencing factors in the underground parking lot showed that concentrations of heavy metals were higher with smaller number of parking spaces and no ventilation system, and older apartments in last paint and cleaning had relatively higher contents of heavy metals than those of recently painted and cleaned.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.3
• /
• pp.384-389
• /
• 2010

Outbreak of contagious diseases to livestock animals is becoming prevalent worldwide and consequently, tremendous numbers of the infected or culled stocks are buried on the ground as the most common disposal method. The buried animals can generate a wide range of detrimental components such as leachate, nutrient salts, and pathogenic bacteria, consequently contaminating the surround environment. This implies that regular investigations are required to monitor any possible detrimental environmental aspect occurred around burial sites. Therefore, the current study was conducted to investigate whether the soil and groundwater nearby the burial sites had been contaminated by the substances originated from the burial sites, which can be applied for the establishment of the ideal burial site construction design and post management scheme. For this, two different burial sites located in Cheonan and Pyeongtaek were selected. Cheonan and Pyeongtaek sites were constructed in 2004 and 2008, respectively and both contained dead poultry infected by avian influenza (AI). Soil and groundwater samples were collected around the sites followed by determination of the nutrient concentrations and bacteria (Salmonella, Camphylobacter, and Bacillus) existence in both soil and groundwater. Some of the soil samples showed higher EC, $NH_4$-N, $NO_3$-N concentration compared to those of the background (control) soils. Also the concentration of $NH_4$-N in some of the groundwater samples appeared to exceed the USEPA guideline value for drinking water (10 mg $L^{-1}$). These results indicated that the soil and groundwater were influenced by the burial site originated nutrients. In the soil, Bacillus was isolated in most soil samples while there were no detections of Salmonella and Camplylobacter. Due to the Bacillus existing mainly as a spore in the soils, it was considered that the frequent detection of Bacillus in the soil samples was attributed to the nutrients originated from the burial sites.

• Journal of Food Hygiene and Safety
• /
• v.34 no.6
• /
• pp.571-575
• /
• 2019

Fungal occurrence during production of dried red pepper was investigated using red pepper samples collected at harvest, before and after washing, and before, during or after drying. Fungal incidence was evaluated by counting the number of fungal colonies grown after incubating random pepper cuts on potato dextrose agar plates. Washing with ground water had no significant effect on reduction of fungal contamination. Fungal increase was observed in some samples, and the insides of washer and containers were contaminated with fungi. Drying caused significant fungal increase regardless of drying method although the fungal incidence after machine drying was lower than that after greenhouse drying. Fungal increase was observed in the samples being dried in a greenhouse and some mycotoxigenic species were also detected. Therefore, the most important control point for fungal contamination during dried pepper production appears to be the drying process, especially in a greenhouse.

• Journal of the Korean GEO-environmental Society
• /
• v.9 no.6
• /
• pp.13-19
• /
• 2008

The objectives of this paper are to investigate states of these open dumping landfills for managing, utilizing and stabilization of a municipal solid waste landfill site in environmentally secure conditions. The result of the physical analysis of the landfill showed that it is composed of between 29.72-63.84% organic matter, 32.88% vinyl plastic. The environmental assessment of the landfill site confirmed that the landfill is at a maturation phase due to 0.18 of $BOD_5$/CODcr of leachate. VS and FS of TS Was respectively 30.37%, 32.34% and C/N ratio was 21.8. Surface water around landfill was BOD 10.7 mg/g, SS 37.8 mg/g, E-Coli 31,157(MPN/$100m{\ell}$) and Ground water was $COD_{Mn}$ 1.13-1.38 mg/g, $NO_3-N$ 1.025-4.075 mg/g. Leachat indicated T-P 0.002-0.028 mg/g, $NH_3-N$ 4.0-21.0 mg/g. The soil contamination of around landfill didn't appear as below of the regulation of Soil Environment Conservation Act. The Landfill Gas was $CH_4$ 13.25%, $CO_2$ 6.17%, H2S and CO was not detected. Also Surface Water was not detected $CO_2$, $CH_4$, $H_2S$, CO.