• The Plant Pathology Journal
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• 제27권4호
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• pp.315-323
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• 2011

Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV and Soybean mosaic virus (SMV) and in transgenic soybean plants expressing SMV helper component-protease (HC-Pro). In many potyviruses, HC-Pro is a potent suppressor of RNA silencing. In plants infected with BPMV alone, accumulation of siRNAs was positively correlated with symptom severity and accumulation of BPMV genomic RNAs. Plants infected with both BPMV and SMV and BPMV-infected transgenic soybean plants expressing SMV HC-Pro exhibited severe symptoms characteristic of BPMVSMV synergism, and showed enhanced accumulation of BPMV RNAs and siRNAs compared to plants infected with BPMV alone and nontransgenic plants. Likewise, SMV HC-Pro enhanced the accumulation of siRNAs produced from a silenced green fluorescent protein gene in transient expression assays, while the P19 silencing suppressor of Tomato bushy stunt virus did not. Consistent with the modes of action of HC-Pro in other systems, which have shown that HC-Pro suppresses RNA silencing by preventing the unwinding of duplex siRNAs and inhibiting siRNA methylation, these studies showed that SMV HC-Pro interfered with the activities of RNA-induced silencing complexes, but not the activities of Dicer-like enzymes in antiviral defenses.

• 한국작물학회지
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• 제49권2호
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• pp.82-88
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• 2004

The objective of this study was to determine the role of storing years with the variation of total phenol and individual phenolic compounds in soybean (Glycine max L.) seeds. The total phenol content varied from 0.36 to 0.42% over four years, with the highest value (0.42%) found at storage for two and three years. Among the nine soybean varieties examined, Daweonkong had the highest total average phenol content (0.58%). The total content of 11 phenolics varied from 730.0 to 1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$ over storage for four years, and the highest concentration (1812.8 $\mu\textrm{g}\;\textrm{g}^{-1}$) was found at storage for two years. Myeongjunamulkong (1465.4 $\mu\textrm{g}\;\textrm{g}^{-1}$) had the highest mean content among the nine soybean varieties. The total content of 11 phenolic compounds measured in this study occupied from 20.96 to 47.73% of the total phenol contents. The highest total phenol contents were in seeds with black coats (5279.4 $\mu\textrm{g}\;\textrm{g}^{-1}$), while the highest concentration of individual phenolic compounds were in seeds with green coats (1419.5 $\mu\textrm{g}\;\textrm{g}^{-1}$). Our study suggests that it may be feasible to improve soybean varieties with high functional substances such as phenolic compounds.

• 한국작물학회지
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• 제41권3호
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• pp.257-265
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• 1996

본 시험은 풋콩 채종기술의 기초자료를 얻고자 미카와시마, 화엄풋콩 및 석량풋콩의 3품종을 1992년 고냉지인 강원도 평창지역에서 생산된 종자와 일본 북해도에서 생산되어 1993년 춘계에 도입된 종자를, 1993년 4월 20일 수원 작물시험장에 채종하여 생육, 병해 및 수량들을 비교 검토한 바 그 주요 결과를 요약하면 다음과 같다. 1. 포장 출현율은 품종간에 차이가 있었고, 채종지간에는 국내고냉지 평창산 종자가 일본산 종자들보다 7% 정도 높았다. 2. 콩 생육경과중에 발생한 미이라병균에 의한 꼬투리 감염, 탄저병, 모자이크바이러스 및 노균병은 채종지간에 차이가 없었다. 3. 채종지간에 풋콩의 생육, 수량구성요소 및 수량성 차이가 없었다. 4. 이상의 결과로서 국내 고냉지에서 풋콩종자를 생산하면 일본산 도입종자에 비하여 출현율이 높고 생육과 수량면에서 저하되지 않는 양질종자의 생산이 가능하다.

• 환경위생공학
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• 제22권1호
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• pp.37-47
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• 2007

In order to improve the Quality of fermented soybean Doenjang was investigated. The samples were prepared and fermented by the koji made with A. oryzae, B. subtilis and B. natto strains, that the product added green tea powder with sanitary canned food. 1. The water content of sample was 50.30-42.14%, pH value 6.03-5.52 and total acidity, 3.01-3.87. 2. The reducing sugar content for samples was 9.35-7.81%, Amino-N, 7.96-9.21%. 3. In the sensory evaluation test, the good sensory qualify that was S-2 sample than to S-1 and S-3. 4. The heavy metals analyzed from sample cans, tin, iron and lead content were 27.31-29.04 ppm, 5.32-5.91 ppm and trace amount.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2557-2560
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• 2010

To assay for antioxidant capacity of natural products considered important in producing human health benefits, a practical and economical method using pellet techniques was developed. A standard visualizing reagent, 1,1diphenyl-2-picryl-hydrazyl (DPPH), was mixed with a water-miscible polyvinyl alcohol (PVA), serving as a solid phase support for the DPPH reagent. A DPPH pellet was prepared by dropping a small volume of the DPPH solution onto PET film, and drying in an oven. The PVA-based DPPH pellet was dissolved into water, in which the water-miscible PVA plays as a non-ionic surfactant to help the DPPH reagent to be dissolved into the solvent. Using the DPPH assay, the antioxidant capacity of water-soluble extracts of black soybean, barley, green tea, and green gram was examined. Among the natural products tested, green tea showed the highest antioxidant capacity. This PVA-based DPPH antioxidant assay can be further applied in the natural food, raw plant material, and health product inspection field.

• 한국농공학회지
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• 제35권1호
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• pp.29-39
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• 1993

A model for estimating daily effective rainfall of upland crops was developed. The infiltration process was described by Green-Ampt infiltration model developed by Chu(1978). The model considers delayed surface ponding and surface detention storage under a uniform soil profile. The Green-Ampt parameters, that is, average hydraulic conductivity and average capillary pressure head on a sandy loam soil were determined from field experiment using Air-entry permeameter developed by Bouwer(1966). The model was verified by comparing measured and simulated surface runoff. The ratios of effective rainfall to total rainfall for red pepper, soybean, sesame and Chinese cabbage were evaluated using Borg's root growth model( 1986) respectively. The followings are a summary of this study results; 1.In a sandy loam soil average hydraulic conductivity was 3.28cm/hr and average capillary pressure head was 3.00cm. 2.The root growth of upland crops could be expressed by Borg's root growth model successively. 3.The measured and simulated surface runoff was agreed well with each other. 4.As the rainfall amount was increased, the ratio of effective rainfall to total rainfall was decreased exponentially till a certain growing period.

• 한국지역사회생활과학회지
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• 제20권3호
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• pp.319-330
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• 2009

This study investigated the field application and the acceptance test of the meal service for the elderly (${\ge}$ 65 years) community halls in Jeonnam and Gyeongbuk. The acceptance scores of bean rice and glutinous rice were the highest among the tested cooked rice varieties in Jeonnam and in Gyeongbuk, respectively (P<0.05). The acceptance scores of tofu soybean paste soup and spinach bean paste soup were the highest among different soups, in Jeonnam and Gyeongbuk, respectively. The scores of menu 5 (bean rice, tofu and bean paste soup, pan-fried pork, green laver salad, kimchi, soy milk) and menu 1 (cereal rice, fermented soy bean soup, hard-boiled quail's egg, crown daisy salad, kimchi, mandarin) were the highest in Jeonnam and Gyeongbuk, respectively, in terms of overall acceptance (P<0.05). The average cost of each meal in two provinces was $2012{\pm}323$ won. The amount of leftovers from menu 5 (Bean rice, Tofu and soybean paste soup, Pan-fried pork, Seasoned green laver, Kimchi, Soy milk) was the lowest in Jeonnamin, while the one from menu 2 (rice, leaf beet and soybean paste soup, sated vegetables with potato noodle, hard-boiled potato, kimchi, cherry tomato) was the lowest in Gyeongbuk.

• 한국육종학회지
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• 제40권3호
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• pp.318-323
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• 2008

풋콩용 신품종 "녹원"은 1996년 황색 대립이고 극조숙인 큰올콩을 모본으로 하고 검정 대립 수집 유전자원인 향남 1호가 교배된 조합이다. '97 ~2001년도에 걸쳐 $F_1-F_5$세대를 계통육종법으로 전개하여 풋콩 특성이 유망하여 선발된 SS96425-2B-11-4-1계통으로 생산력검정시험에서 수확시기가 빠르고 줄기가 짧으며 도복에 강한 대립 풋콩 다수성 계통으로 유망하여 선발된 밀양 153호이다. 풋콩 식미가 우수하며 가지 형태로 유통이 가능한 풋콩용 품종으로 육성된 "녹원"의 주요특성은 다음과 같다. 1. 유한신육형이며 꽃은 백색이고 잎은 환형이며 모용은 회색이다 풋협색은 진한 녹색이며 성숙 종피색은 녹황색을 띠며 종자의 배꼽은 갈색이며 성숙 종자모양은 구형이다. 2. 개화기는 화엄풋콩보다 일 빠른 6월 20일이며 풋협수확기는 4일 늦은 8월 7일이다. 경장은 34 cm로 화엄풋콩 보다 11 cm 짧은 단경 품종이다. 3. 도복과 화엄풋콩보다 강하며 내습성과 콩나방 피해율은 비슷하다. SMV는 포장에서 강하였으나 유묘검정에서는 3개 strain에 이병 되었다. 4. 화엄풋콩과 협장은 같고 협폭은 다소 좁으며, 풋콩 100 립중은 75.4 g으로 5.4 g 더 무거운 대립종이다. 500 g 풋협 수는 158개이며 2-3협의 비율은 80.2%로 높다. 5. 조단백질 함량은 39.4%이고 조지방 함량은 17.3%이며 sucrose함량은 5.7%로 화엄풋콩보다 다소 낮았다. 고소한 맛이 우수하고 비린 향도 덜하여 전체기호도가 화엄풋콩보다 우수한 식미를 보인다. 6. 전국 6개소 지역적응시험 결과 풋협 수량은 화엄풋콩보다 14% 증수한 ha당 11.44톤이었다.

• 한국작물학회지
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• 제68권3호
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• pp.114-120
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• 2023

갈색종피와 녹색자엽을 가진 품종 및 유전자원의 성숙 종실에는 눈 건강에 유익한 루테인 성분과 항산화 효과를 가진 플라보노이드 성분이 많이 함유되어져 있다. 그러나, 렉틴, 7S α′ subunit, 리폭시게나제 및 쿠니츠 트립신 억제제(KTI) 단백질 같은 항영양 성분이 존재한다. 이러한 항영양 성분을 불활성화시키기 위하여 콩 식품 제조 및 가공시 고온 및 첨가제 처리가 필요하지만 여러 가지 단점이 수반된다. 따라서, 갈색종피와 녹색자엽이면서 성숙 종실에서 렉틴, 7S α′ subunit, 리폭시게나제 및 KTI의 4가지 단백질이 모두 부재한 tetra null 유전자형(lecgy1lox1lox2lox3ti)을 가진 계통을 선발하기 위하여 본 연구가 진행되었다. 4개의 품종과 1개의 유전자원을 이용하여 육종집단 창성을 위한 두 모본이 선발되었다. 전체 58개의 F₂ 식물체로부터 DNA 마커를 이용하여 lele 유전자형을 가진 개체가 선발된 후 갈색종피와 녹색 자엽이면서 7S α′ subunit 단백질이 부재한 F3 종자가 선발되었다. 선발된 F₃ 종자는 F₃ 식물체를 거쳐 3개의 계통으로 육성되었다. 3개의 선발 계통(S1, S2, S3)에 대하여 F₆ 종자에서 렉틴, 7S α′ subunit, 리폭시게나제 및 KTI의 4가지 단백질에 대한 유전적 부재가 검정되었다. 3개의 선발 계통은 갈색종피, 녹색자엽 및 흰색배꼽을 가지고 있으며 백립중은 26.4-30.9 g으로 대조품종인 '청자3호'의 36.0 g보다 작았다. S2 선발 계통은 백립중이 30.9 g으로 대립이며 콩에서 항영양성분으로 알려진 lectin, 7S α′ subunit, lipoxygenase 및 KTI의 4가지 단백질 모두 부재한 유색콩 품종육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다.

• 복식문화연구
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• 제17권6호
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• pp.1129-1140
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• 2009

The pine needles has been used as medicines and it is using as dyeing as well as food. It is distributed through 50% in Korean forest. The pine needles is related to antimicrobial activity, however, dyeing properties of the extract components and effects of them are not well known yet. To examine dyeing properties of the pine needles extract, various fabrics(nylon, silk, wool, and soybean) were dyed under different dyeing conditions, and mordanted with one of five mordants(Al, Sn, Fe, Cr, and Cu). Dye uptake, Colors and Colorfastness of the dyed fabrics were measured. By the K/S values, dye uptake of all the dyed fabrics enhanced as increasing dyeing temperature and dyeing time. The highest K/S values were obtained from the protein fiber(wool and silk) fabrics dyed with water extract at $100^{\circ}C$, and with ethanol extract at $90^{\circ}C$ for 80 minutes. Colors of the dyed fabrics showed variety of yellow, brown and green colors. Colors changed by using Cu, Fe and Cr mordants: Cr-mordanted fabrics into light green, Fe-mordanted fabrics into reddish brown, and Cu-mordanted fabrics into deep green in ethanol extraction. Dry-cleaning fastness of the dyed fabrics presented good to excellent except wool fabrics dyed with ethanol extract. Washing fastness of the dyed nylon and soybean fabrics were good to excellent but wool and silk fabrics showed average grades. Most dyed fabrics were poor to light fastness.