• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
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• pp.6-6
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• 2010

Bacteria of the Bacillus sp. are well known to possess antagonistic activity against numerous plant pathogens. In the present study, 11 Bacillus sp. were isolated from the brackish environment and assayed for antagonistic activity under in vitro and in vivo conditions. Among the 11 isolates tested, 9 isolates effectively inhibited the growth of various plant pathogens, namely Phytophthora capsici, Phytophthora citropthora, Phytophthora citricola, Phytophthora sojae, Colletotricum coccodes, Colletotricum gloeosporioides, Colletotricum acutatum, Rhizoctonia solani, Fusarium solani, Fusarium graminearum, Pyricularia sp. and Monilina sp. The effective isolates were further screened for Phytophthora blight suppression in Capsicum annuum L. under green house conditions. The isolate SB10 exhibited the maximum (72.2%) reduction in disease severity. The antifungal compounds from the isolate were isolated and characterized. The isolated compounds exhibited high thermo stability ($100^{\circ}C$ for 30 min). Matrix-Assisted Laser Desorption Ionization-Time of Flight investigation of the antifungal compounds revealed three lipopeptide complexes, the surfactins, the iturins, and the fengycins.

• 한국결정성장학회지
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• 제5권2호
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• pp.94-99
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• 1995

${\mu}-PD KLN$법에 의해 육성한 KLN 단결정은 적색 레이저를 이용한 제2 고조파 발생(SHG) 특성 평가에 의해 noncritical phase-matching(NCPM)의 파장의 균일성과 우수한 온도 특성을 갖고 있음을 확인하였다. 또한 융액의 조성을 변화시키면서 성장된 결정의 NCPM 파장 측정에 의해 조성과 SHG 특성 사이에는 민감한 상호관계가 있음을 알 수 있었다. 이로부터 원료의 조성제어를 통한 ${\mu}-PD KLN$은 청색에서 녹색파장을 걸치는 넓은 범위에서의 선택적인 SHG 응용이 기대된다.

• The Plant Pathology Journal
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• 제29권1호
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• pp.59-66
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• 2013

Colonization studies previously performed with a green-fluorescent-protein, GFP, labeled derivative of Bacillus amyloliquefaciens FZB42 revealed that the bacterium behaved different in colonizing surfaces of plant roots of different species (Fan et al., 2012). In order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to Arabidopsis seedlings. The fates of root colonization in mutant strains impaired in synthesis of alternative sigma factors, non-ribosomal synthesis of lipopeptides and polyketides, biofilm formation, swarming motility, and plant growth promoting activity were analyzed by confocal laser scanning microscopy. Whilst the wild-type strain heavily colonized surfaces of root tips and lateral roots, the mutant strains were impaired in their ability to colonize root tips and most of them were unable to colonize lateral roots. Ability to colonize plant roots is not only dependent on the ability to form biofilms or swarming motility. Six mutants, deficient in abrB-, sigH-, sigD-, nrfA-, yusV and RBAM017410, but not affected in biofilm formation, displayed significantly reduced root colonization. The nrfA- and yusV-mutant strains colonized border cells and, partly, root surfaces but did not colonize root tips or lateral roots.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2012년도 춘계학술발표대회
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• pp.56.2-56.2
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• 2012

Clinically-favored materials for bone regeneration are mainly based on bioceramics due to their chemical similarity to the mineral phase of bone. A successful scaffold in bone regeneration should have a 3D interconnected pore structure with the proper biodegradability, biocompatibility, bioactivity, and mechanical property. The pore architecture and mechanical properties mainly dependent on the fabrication process. Bioceramics scaffolds are fabricated by polymer sponge method, freeze drying, and melt molding process in general. However, these typical processes have some shortcomings in both the structure and interconnectivity of pores and in controlling the mechanical stability. To overcome this limitation, the rapid prototyping (RP) technique have newly proposed. Researchers have suggested RP system in fabricating bioceramics scaffolds for bone tissue regeneration using selective laser sintering, powder printing with an organic binder to form green bodies prior to sintering. Meanwhile, sintering process in high temperature leads to bad cost performance, unexpected crystallization, unstable mechanical property, and low bio-functional performance. The development of RP process without high thermal treatment is especially important to enhance biofunctional performance of scaffold. The purpose of this study is development of new process to fabricate ceramic scaffold at room temperature. The structural properties of the scaffolds were analyzed by XRD, FE-SEM and TEM studies. The biological performance of the scaffolds was also evaluated by monitoring the cellular activity.

• 설비공학논문집
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• 제25권12호
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• pp.674-678
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• 2013

We present a non-invasive technique to the measure temperature distribution in nano-sized porous thin films by means of the two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence induced by the green line of a mercury lamp with the makeup of optical filters was measured on two separate color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows for absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. To measure temperature fields, Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescent dye was absorbed into these porous thin films. The calibration curves of the fluorescence intensity versus temperature were measured in a temperature range of $10-60^{\circ}C$, and visualization and measurement of the temperature field were performed by taking the intensity distributions from the specimen for the temperature field.

• Bulletin of the Korean Chemical Society
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• 제30권7호
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• pp.1521-1525
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• 2009

Thermosensitive block copolymers of ethylene oxide and N-isopropylacrylamide (NIPAM) were synthesized. A five armed star shape oligo(ethylene oxide) initiator with a cyclotriphosphazene core was prepared and used for the atom transfer radical polymerization (ATRP) of NIPAM. The lower critical solution temperatures (LCSTs) of the copolymers were 36 to 46 ${^{\circ}C}$, higher than that of PNIPAM (32 ${^{\circ}C}$), depending on their molecular weights. The copolymers were soluble in water below the LCSTs but formed micelles above the LCSTs. The thermosensitive micellization behaviors of the polymers were investigated by fluorescence spectroscopy. With increasing the temperature of an aqueous solution of P2 and pyrene above the LCST, the peak of 333 nm red-shifted to appear around 339 nm and its intensity increased significantly, indicating the micelle formation. The transfer of pyrene into the micelles was also confirmed by a confocal laser scanning microscope. The fluorescence image obtained from P2 in an aqueous pyrene solution exhibited a green emission resulting from the pyrene transferred into the micelles. Salt effects on the solubility of the copolymers in an aqueous solution were investigated. The LCST of P2 decreased sharply as the concentration of sodium chloride increased, while decreased slowly with potassium chloride.

• 반도체디스플레이기술학회지
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• 제7권1호
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• pp.11-16
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• 2008

Confocal scanning microscopy is a widely used technique for three dimensional measurements because it is characterized by high resolution, high SNR and depth discrimination. Generally an image is generated by moving one optical probe that satisfies the confocal condition on the specimen. Measurement speed is limited by movement speed of the optical probe; scanning speed. To improve measurement speed we increase the number of optical probes. Specimen region to scan is divided by optical probes. Multi-point information each optical probe points to can be obtained simultaneously. Therefore image acquisition speed is increased in proportion to the number of optical probes. And multiple optical probes from red, green and blue laser sources can be used for color imaging and image quality, i.e., contrast, is improved by adding color information by this way. To conclude, this technique contributes to the improvement of measurement speed and image quality.

• Corrosion Science and Technology
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• 제10권2호
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• pp.60-64
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• 2011

Lean duplex stainless steels have been developed in Korea for the purpose of being used in the seawater systems of industry. There are also many important seawater systems in nuclear power plants. These systems supply seawater to cooling water condenser tubes, heat exchanger tubes, related pipes and chlorine injection systems. The flow velocity of some part of seawater systems in nuclear power plants is high and damages of components from corrosion are severe. The considered lean duplex stainless steels are STS329LD (20.3Cr-2.2Ni-1.4Mo) and STS329J3L (22.4Cr-5.7Ni-3Mo) and PRENs of them are 29.4 and 37.3 respectively. Physical, mechanical and micro-structural properties of them are evaluated, and electrochemical corrosion resistance is measured quantitatively in NaCl solution. Critical Pitting Temperatures (CPT)s are measured on these alloys and pit depths are evaluated using laser microscope. Long period field tests on these alloys are now being performed, and some results are going to be presented in the following study.

• 한국수소및신에너지학회논문집
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• 제23권5호
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• pp.538-544
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• 2012

The bubbles made of hydrogen and oxygen gases producted by electrolysis disturb the electrolysis, but the behavior of these bubbles in the electrolysis stack isn't clearly defined. In order to study on the behavior of bubbles in the flow pattern of the meshes type separator, the flow visualization experiment was performed by using of a visible alkaline electrolysis stack and a stereoscopic microscope. As the results, a fine size bubbles adhered to the separator's surface in the electrolyte solution have grown large sized bubbles until each bubble's buoyance is lager than the sum of surface adhesion force and weight. And then the large bubbles flow into the upper area of the separator. Also, as wide area of the separator have been occupied by various sized bubbles, the electrolysis efficiency is declined.

• 한국광학회지
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• 제7권2호
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• pp.120-128
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• 1996

1at.% Er$^{3+}$ :LaF$_{3}$와 1at.%Er$^{3+}$ :YAlO$_{3}$ 결정체에 대해 780-820nm에서 펌핑하여 적색, 녹색 및 청색이 상방전환 형광 발생을 상온과 14K에서 연구하였다. 두 재료에 대해 Er$^{3+}$ 이온의 $^{4}$I$_{9}$ 2/준위를 펌핑하기 위해 연속 출력의 티나튬 사파이어 레이저를 사용하였다. 이러한 $^{4}$F$_{9}$ 2/, $^{4}$S$_{3}$2/, $^{2}$P$_{3}$2/로부터 상방전환의 형광 발생 과정이 다단계 흡수와 교차 완화의 에너지 전달에 의한 것임을 스펙트럼의 분석을 통행 밝혀내었다.