• The Plant Pathology Journal
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• v.38 no.6
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• pp.679-684
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• 2022

Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M. oryzae, the direct extraction of M. oryzae genomic DNA from infected rice tissue would be useful alternative for rapid monitoring of changes of avirulence genes without isolation and cultivation of the pathogen. In this study, a fast, low-cost and reliable method for DNA preparation of M. oryzae from a small piece of infected single rice leaf or neck lesion was established. This single step method only required 10 min for DNA preparation and conventional chemical reagents commonly found in the laboratory. The AvrPik and AvrPi9 genes were successfully amplified with the prepared DNA. The expected DNA fragments from 570 bp to 1,139 bp could be amplified even three months after DNA preparation. This method was also suitable for DNA preparation from M. oryzae strains stored on the filter paper. All together these results indicate that the DNA preparation method established in this study is reliable, and could meet the basic needs for polymerase chain reaction-based analysis of M. oryzae.

• Journal of Korean Society of Rural Planning
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• v.14 no.4
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• pp.109-119
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• 2008

The objectives of this study are to develop a green tourism potential evaluation method with rural amenity and demand of citizen. The new index which was named GPD(green tourism potential degree) is designed to propose the green tourism potential of rural areas using spatial analysis of geographic information system and spatial interaction of gravity model. And in order to evaluate the green tourism potential with supply side and demand side, two indices were defined; One is green tourism demand degree(GDD) which is developed to quantify a demand side potential by the analysis of urban population and urbanization index, and the other is green tourism attraction degree(GAD) which is developed to quantify a supply side potential by the analysis of rural amenity values using AHP algorithm, based on opinion of related experts. The developed method was applied to a part of Kyounggi province, Seoul and Incheon. All the study area's GAD, GDD and GPD were assessed and the proposed green tourism potential evaluation method could be used in developing rural development plans and green tourism policies considering spatial interaction with citizen and green tourism resources.

• Journal of Agricultural Extension & Community Development
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• v.18 no.4
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• pp.961-981
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• 2011

This study selected Joongchon Village in Gimje-si as a target, in order to apply green village design regarding regional circumstances and features within the local government, in making green village as an efficient scheme in aspect of locality for 'Low Carbon, Green Growth'. Subsequently, we conducted survey and analysis. Natural circulation-based green village of Joongchon Village in Gimje-si is a low carbon green village, based upon eco-friendly cattle shed. Even though it is the fact that the initial costs of an eco-friendly cattle shed are rather high, it is the long-term low carbon green technology that can transform livestock night soils into resources, provide them to agricultural farms, independently supply energies from by-product, and produce energies additionally. Therefore, Joongchon Village in Gimje-si is the good example of green village, applying the design which actively utilizes discharged by-product from cattle shed, so natural circulation and energy production are able as an eco-friendly green technology.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2005.10a
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• pp.208-222
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• 2005

• Bulletin of Food Technology
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• v.22 no.4
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• pp.710-717
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• 2009

• Journal of Korea Water Resources Association
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• v.33 no.S1
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• pp.925-930
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• 2000

• Proceedings of the Plant Resources Society of Korea Conference
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• 1996.09a
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• pp.70-71
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• 1996

• Magazine of RCR
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• v.15 no.4
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• pp.48-57
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• 2020

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.5
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• pp.111-118
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• 2011

With increasing interest in health promotion and quality of life, growing attention has been focused on the beneficial effects of urban green area. However, very few evidence-based approaches have been conducted on the health-related benefits of urban greenery. Therefore, this study examined the health-related benefits of green areas using physiological and psychological indices to obtain evidence-based data on these benefits. Twenty male university students were selected as subjects. Data were collected when participants viewed landscapes in a green area or an urban area for fifteen minutes. This research was reviewed and approved by the Ethics Committee of School of Medicine, Chungnam National University. Physiological data in the green area revealed significantly decreased heart rates, significantly increased high-frequency value of heart rate variability, an index of parasympathetic activity, and reduced salivary cortisol concentration, a stress hormone, compared to the urban area. Psychological tests showed the green area significantly reduced the negative mood state and psychological symptoms, and significantly increased the positive mood state. Our data provided evidence for the health-related benefits of green areas, and the findings of this study support that green areas can play a critical role in health promotion for urban residents, by positively affecting autonomic nervous and endocrinal activities.

• Journal of Mushroom
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• v.15 no.4
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• pp.259-263
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• 2017

In this study, we investigated the morphological characteristics and antioxidant ability of mushroom cultivar Lentinula edodes 'Nongjingo' fruiting bodies after exposure to various light conditions. Color differences between mushrooms treated with mixtures of LED light revealed that mushrooms displayed lighter color shades when compared to the control group (fluorescent light treated mushrooms). Redness increased and yellowness decreased after exposure to all treatments other than the fluorescent control. Measurement of growth characteristics of 'Nongjingo' fruiting bodies showed increases after exposure to all mixed LED treatments. In addition, the uniformity of fruiting bodies was higher when using LED light compared to fluorescent light. The measurement of stem diameters did not show a significant difference between the treatments, however, diameters were slightly larger with exposure to white-green LED. Moreover, stem length was longer in the mixed LED treatments when compared to those exposed to fluorescent light. Examination of the ratio of stem diameter to stem length revealed that the diameter of the stem was greater than the length. The antioxidant activity of water extracts made from Nongjingo fruiting bodies grown under mixed LED conditions was compared to those from mushrooms grown under fluorescence light conditions. The highest antioxidant activity was observed from mushrooms treated with white LED; however, no significant difference was found between mushrooms exposed to white-green LED compared to white-blue LED. The treatment showed higher antioxidant ability than vitamin C. Our results confirm that treatment of white LED and white-blue LED affects the growth and antioxidant ability of Nongjingo mushroom fruiting bodies.