• 대한지역사회영양학회지
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• 제8권4호
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• pp.595-602
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• 2003

This study was carried out to investigate the status of the utilization of internet and dietary information by elementary school dietitians (total = 201) The results were summarized as follows: All subjects used the internet regularly and the major purpose they mentioned for using it was ″data search″ (72.3％). Those having a frequency of using the internet 6 to 7 times per week were 46.7％ of the subjects and the majority indicated the duration of their use of the internet was ″〈 2 hours″ (68.5％). They mainly used the internet at ″school″ (81.8％) , and their favorite search engines were ″Daum″ (41.1％) and ″Yahoo″ (34.7％). They stated that the organization that offered reliable internet information were those ″related to society and organizations″ (36.9％) and ″educational institutions″ (33.8％). Ninety-five percent of the subjects searched for dietary information mainly ″for public affairs″ (80.0％) Their degree of satisfaction when searching for information was high (78.7％). Those dissatisfied with internet sites when searching for dietary information give the following reasons: ″poor information″ (39.1％) and ″slow updates″ (25.4％). They acquired information on nutrition and health management mainly through ″the internet″ (63.6％) The most frequently requested information about public affairs was on ″Materials for nutritional education″ (38.6％) and ″up-to-date nutritional information″ (16.0％) . The desirable update periods for websites was ″1 -3 months″ (51.8％) and ″ < 1 month″ (30.6％), and the favorite site colors were ″blue″ (37.8％) and ″green″ (37.8％). The results of this study showed that, although the internet usage was high, the dietitians were dissatisfied with the information obtained. Therefore, the information donors should find out what kind of dietary information is needed by elementary school dietitians. (Korean J Community Nutrition 8(4) : 595-602, 2003)

• 유통과학연구
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• 제10권7호
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• pp.23-31
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• 2012

The purpose of this study is to investigate existing world economic science theories and concepts of sustainable development as well as create original system of criteria and indicators to assess potential and conditions for sustainable development from a regional perspective and based on the above justify proposals for public authorities to improve methods of economic regulation for regional development. To achieve the goal evolution process of existing theories and concepts of sustainable development and "green growth" have been studied in terms of its adaptation to the spatial development of Kazakhstan, comparative dynamics of natural resources consumption in the Asia-Pacific region and Kazakhstan were investigated as well. Methodology. World best theory and practice methods for assessment of the level of sustainable development of the country and some of its territories were also studied. We selected the best system of criteria and indicators for assessment of economic, social and environmental sustainability of regional systems. Methods offered in this article are based on traditional and new factors of economic development and conditions for operation of regional systems. Indicators are chosen with a glance to basic goals for future development of regions in Kazakhstan. It is recommended to use two levels of methodology offered by author, namely national and regional, and at the local level later (districts, municipalities, cities). The results. Method offered by the author has been tested based on materials and statistics of regions in Kazakhstan. Suitability and usability of the proposed system of criteria and indicators for measuring of economic, social and environmental sustainability of regional systems was proved. Based on this analysis it was found out that there are regions in Kazakhstan that differ from each other by most of key indicators of sustainable regional development and this tendency is increasing. Conclusions and recommendations. As a result of this analysis, the authors have substantiated a number of proposals on methods of analysis to be used, improvement of methods of regional development, implementation of institutional reforms, as well as improvement of regional statistics. This article provides recommendations that can be successfully used in the management practices by public authorities to implement a new regional strategy focused on sustainable development in future.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2051-2059
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• 2016

To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was $3,219{\pm}755Hz$, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.

• 지질공학
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• 제20권1호
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• pp.1-12
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• 2010

본 연구에서는 인천 해안 LPG공동의 수리화학적 안정성을 평가하기 위해 공동 유출수 및 주변 지하수에 대한 수리지구화학적 및 미생물학적 특성을 평가하였다. 수질특성을 파악하기 위하여 2007년 2월, 5월, 8월, 10월에 걸쳐 시료채취 및 수질분석을 실시하였다. 프로판공동 지하수의 경우 pH는 8.1~12.4의 높은 값을 보였으며 이는 공동건설 및 관측정설치에 사용된 시멘트 그라우팅 물질의 용해영향으로 사료된다. 전기전도도는 시료채취 시기별로 큰 차이를 보였으며, 음의 산화환원전위는 유출수 및 지하수가 환원환경에 있음을 지시하였다. 수질분석결과 유출수 및 지하수의 수질유형은 모두 Na-Cl type으로 나타났으며 이는 해수의 영향으로 판단된다. 호기성세균, 혐기성세균, 점액질세균, 황산환원세균 모두 500 CFU/mL 미만으로 낮은 값을 보였으며 급격한 미생물 성장은 관찰되지 않았다. 현재로선 공동 운영에 지장을 주는 특이적 수리화학적 현상이 발견되지는 않았으나 지속적인 모니터링이 필요하다.

• 한국가구학회지
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• 제21권3호
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• pp.216-228
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• 2010

Recently, there is a new issue, among the contemporary people, for new life style, such as green design and well being. This trend brought up the necessity that there should be alternatives for interior spatial design. In order to catch up with these new issues, the new convenient and environment friendly methods are in need. Space composition using setting-up is skill that can express both the structural aspect and esthetic because it represents traditional beauty into the contemporary age through the structural rigidity and formal beauty. Also the lumber, as main materials for setting up, is in line with well being life style and environment friendliness. The construction of structure by setting-up has advantages in terms of the reuse and the convenience in that the construction of structure is adjustable according to environment. And setting-up has enough plasticity not only because of its own role as linking the objects but also because of being framed by itself. Therefore setting-up will be a design element, if it is expressed outward. Thus, this study aims to give a guide line about how to apply the result from the evaluating that "what is the most suitable setting-up" and "what is the most suitable detail setting-up", based on that structural rigidity, decorativeness and the ease of works. As a result of evaluation, the most excellent types of setting-up in terms of structural rigidity are "Jangbu" and "Panjae" and "Yeongui". The most excellent types of setting-up in terms of decorativeness are "Panjae" and "Yeongui", and the most excellent type of the ease of work is "Mat". And also the most applicable detail setting-up for the utilization of spatial composition is proposed.

• 동의생리병리학회지
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• 제24권4호
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• pp.586-591
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• 2010

Chemokine and Growth Factor are major mediumtors of immuno-inflammatory pathway. The purpose of this study is to investigate whether productions of Chemokine and Growth Factor in lipopolysaccharide (LPS)-induced mouse macrophage RAW 264.7 cells are modulated by Gallic acid (GA), which is easily founded in tannin-containing natural materials such as red wine, green tea, grape juice, and Corni Fructus. Productions of Chemokine and Growth Factor were analyzed by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. At first, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, the antibody-conjugated beads were added and incubated for 30 minutes. After incubation, detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Based on fluorescence intensity, concentrations of Chemokine and Growth Factor were determined. The results of the experiment are as follows. GA significantly inhibited the production of interferon-inducible protein (IP)-10, keratinocyte-derived chemokine(KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). GA significantly inhibited the production of monocyte chemoattractant protein-1(MCP-1) and macrophage-colony stimulating factor(M-CSF) in LPS-induced RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). GA diminished the production of granulocyte macrophage-colony stimulating factor (GM-CSF) in LPS-induced RAW 264.7 cells. But GA did not show the inhibitory effect on the production of leukemia inhibitory factor (LIP) and macrophage inflammatory protein (MIP)-2 in LPS-induced RAW 264.7 cells. These results suggest that GA has the immuno-modulating activity related with its inhibitory effects on the production of IP-10, KC, MCP-1, VEGF, and M-CSF in LPS-induced macrophages.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.273-281
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• 2011

Diverse epigenetic phenotypes are frequently found during research on transgenic plants. To understand the factors underlying such diversity, hundreds of independent 35S-GFP transgenic N. benthamiana plants were analyzed. The diverse GFP-expression phenotypes of the transgenic plants were classified into three major types based on the GFP expression patterns and their response to 35S-GFP agroinfiltration: steady-green, silenced and non-uniform phenotype. The non-uniform phenotype was further sub-divided into five minor phenotypes: variegated, red-dropped, on-silencing, partitioned and misty, according to the distribution of GFP expression on the leaves. Many of transgenic plants continuously generated diverse phenotypes over several generations despite the transgene identity. Such epigenetic GFP phenotyping was found to be the result of spontaneous transgene silencing mediated by either or both of post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS). This finding was verified by the detection of 21- and 24-nt small interfering RNA (siRNA) molecules, and DNA methylation in the transgenic plants that showed repeated epigenetic variation. Agroinfiltration demonstrated that irregular distribution of GFP on a leaf was the result of erratic transgene silencing, and the technique also proved to be a rapid and effective method for selecting fully silenced plants within 3 days. Furthermore, two novel phenotypes described are potential materials for in-depth investigations into the genes and mechanisms responsible for spontaneous transgene silencing.

• 한국정밀공학회지
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• 제16권6호
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• pp.52-57
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• 1999

Funtional metal prototypes are often required in numerous industrial applications. These components are typically needed in the early stage of a project to determine form, fit and function. Recent R/P(Rapid Prototyping) part are made of soft materials such as plastics, wax, paper, these master models cannot be employed durable test in real harsh working environment. Parts by direct metal rapid tooling method, such as laser sintering, by now are hard to get net shape, pores of the green parts of powder casting method must be infiltrated to get proper strength as tool, and new type of 3D direct tooling system combining fabrication welding arc and cutting process is reported by song etc. But a system which can build directly 3D parts of high performance functional material as metal part would need long period of system development, massive investment and other serious obstacles, such as patent. In this paper, through the rapid tooling process as silicon rubber molding using R/P master model, and fabricate wax pattern in that silicon rubber mold using vacuum casting method, then we tranlsated the wax patterns to numerous metal prototypes by new investment casting process combined conventional investment casting with rapid pototyping & rapid tooling process. with this wax-injection-mold-free investment casting, we developed new investment casting process of fabricating numerous functional metal prototypes from one master model, combined 3-D CAD, R/P and conventional investment casting and tried to expect net shape measuring total dimension shrinkage from R/P part to metal part.

• Journal of the Korean Wood Science and Technology
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• 제19권4호
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• pp.34-42
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• 1991

Protection of tropical forest affects on significant reduce of tropical hardwood supply, and softwood resources will be increasingly important for the timber security in Korea. U.S. softwood log was most favorite species for Korean softwood log importers in overall import conditions except price stablization and consistency of export policy. Reduced export volume from Pacific Northwest to Korean market has been immediately replenished by rediata pine from New Zealand and Chilean plantation. Siberian timber will hardly play major roles in Korean timber market unless budding structure. softwood plywood and softwood furniture uses are enhanced. Recent rapid rise of labor cost and reducing tariff rrate in Korea provided better opportunities for import lumber in building materials market. Dry dimension lumber was relatively profitable when processed from import U.S. soft-wood log while green lumber was favorable products processed from radiata pine log in Korean lumber market. This means U.S. softwood lumber would have better opportunity to market for '2${\times}$'4 studs when wood frame housing is introduced. On the other hand while radiata pine is competitive on temporary construction lumber such as supporter and concrete forming frame in Korea. Shortage of raw material for the new capacity of board plants in Korea will be it bottle neck. Major log export countries to Korea as U.S. New Zealand and Chile showed high trade intensity indices of composite hoard produces for Korean market. As Korea efforts to diversify import sources, and tariffs are reduced to 8% as scheduled by 1994. countries of scoring higher comparative advantages as Portugal. Brazil, Austria as well as New Zealand will have better opportunity to penetrate into promised Korean composites hoard market.

• Journal of the Korean Wood Science and Technology
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• 제24권2호
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• pp.46-54
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• 1996

This study was carried out to investigate the vacuum drying characteristics of 50mm- and 75mm- thick tree disks of some domestic softwoods for substituting the conventional hardwoods as the materials for wood crafts. Though the elapsed drying times from green to in-use moisture content(MC) were largely shortened by vacuum drying, the tree disks treated by EWS couldn't be dried to in-use MC, and so sapwood stains also were occurred severely. We suggest EWS treatment is undesirable for the species with very high sapwood portion or vacuum drying with hot water circulation heating system. Heart checks were slight, but sapwood checks, which have never been trouble in drying process of tree disks, were severe. For the reasonable explanation it is suggested MC differences between sapwood and heartwood were large and most of tree disks had already no barks before drying test. Ginkgo was vacuum-dried with very slight drying defects such as heart checks, sapwood checks. V-cracks and sapwood stains. In Korean red pine and pitch pine V-cracks were severely occurred. And it was found the special feature that most of these defected tree disks contained several V-cracks within one tree disk. It can be considered as the causes that the region of sapwood was defected by the several checks at the early drying stage because of the steep MC gradient along the radial direction, and then at the later drying stage the drying stresses due to differential shrinkage were concentrated on these brittle spots.