• 한국광학회지
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• 제11권2호
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• pp.80-84
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• 2000

Diamond turning machine으로 알루미늄을 가공하여 f/1.5, 구경 50mm인 포물명경을 제작하였다. 이진위상(binary phase) 홀로그램으로 포물면경 현상측정을 위한 computer generated hologram(CGH)을 encoding하였으며 staircase encodling 방법으로 간섭무늬 곡선의 직선근사를 하였다. 간섭무늬 데이터를 포스트스크립트(PostScript) 파일로 변환한 후 CGH 원도를 레이저 프린터로 확대 출력하고 축소 촬영하여 CGH를 제작하였다. 측정부(viewing arm)에 CGH가 위치하는 Twyman-Green 간섭계를 구성하여 포물면경의 표면형상을 측정하였다. 측정결과를 직접표면형상측정법 및 간섭계를 이용한 자동시준법의 측정 결과와 비교하고 오치를 분석하였다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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• pp.843-845
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• 2009

A polymeric host for triplet emitters composed of N-alkylcarbazole and tetramethylbenzene units was successfully synthesized. Efficient energy transfer was observed between this polymeric host and green phosphorescent dyes. The device fabricated using 5 wt% green 1 in the polymeric host as the emitting layer showed the best performance. Thin films of this host-guest system, exhibiting clear stripe patterns could be prepared through the LITI process. The patterned films were then used to fabricate electrophosphorescent devices, which show performance characteristics similar to those of spin-coated devices. The new host material is a good candidate to be used in polymer-based full-color electrophosphorescent light-emitting displays.

• 대한기계학회논문집
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• 제14권1호
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• pp.157-171
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• 1990

본 연구에서는 저탄소강 판재가 소성변형을 하면 열확산계수가 변화하는가를 구명하려는 것이다. 재료의 소성변형은 압연법을 이용하며, 고온압연, 열간압연 및 냉간압연법으로 재료를 제작하여, 압연률을 변화시키면서 압연방법 및 압연률에 따르 는 변화를 규명하려 한다. 열확산계수의 측정은 레이저 섬광법으로 하며 온도를 실 온에서 500.deg. C 근처까지 변화시키면서 측정한다.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1079-1082
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• 2007

Chromosome microdissection and the reverse FISH technique is one of the most useful methods for the identification of structurally abnormal chromosomes. In particular, the laser microbeam microdissection (LMM) method allows rapid isolation of a target chromosome or a specific region of chromosomes without damage of genetic materials and contamination. Isolated chromosomes were directly amplified by the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), and then the FISH probes labeled with spectrum green- or spectrum red-dUTP were generated by nick-translation. Whole chromosome painting (WCP) probes were successfully generated from only 5 copies of the chromosome. With this method, we produced 24 WCP probes for each human chromosome. We also tried to characterize a marker chromosome, which seemed to be originated from chromosome 11 on conventional banding technique. The marker chromosomes were isolated by the LMM method and analyzed by reverse FISH. We elucidated that the marker chromosome was originated from the short arm of chromosome 5 ($5p11{\to}pter$). A fully automated and computer-controlled LMM method is a very simple laboratory procedure, and enables rapid and precise characterization of various chromosome abnormalities.

• Clinical Endoscopy
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• 제51권6호
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• pp.513-526
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• 2018

White light imaging (WLI) may not reveal early upper gastrointestinal cancers. Linked color imaging (LCI) produces bright images in the distant view and is performed for the same screening indications as WLI. LCI and blue laser imaging (BLI) provide excellent visibility of gastric cancers in high color contrast with respect to the surrounding tissue. The characteristic purple and green color of metaplasias on LCI and BLI, respectively, serve to increase the contrast while visualizing gastric cancers regardless of a history of Helicobacter pylori eradication. LCI facilitates color-based recognition of early gastric cancers of all morphological types, including flat lesions or those in an H. pylori-negative normal background mucosa as well as the diagnosis of inflamed mucosae including erosions. LCI reveals changes in mucosal color before the appearance of morphological changes in various gastric lesions. BLI is superior to LCI in the detection of early esophageal cancers and abnormal findings of microstructure and microvasculature in close-up views of upper gastrointestinal cancers. Excellent images can also be obtained with transnasal endoscopy. Using a combination of these modalities allows one to obtain images useful for establishing a diagnosis. It is important to observe esophageal cancers (brown) using BLI and gastric cancers (orange) surrounded by intestinal metaplasia (purple) and duodenal cancers (orange) by LCI.

• 구강회복응용과학지
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• 제40권2호
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• pp.55-63
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• 2024

목적: 이 연구의 목적은 거친 티타늄 표면에 형성된 Staphylococcus aureus 바이오필름에 대해 인도시아닌 그린을 활용한 광역학치료(photodynamic therapy; PDT)의 항미생물 효과를 평가하고, 이를 임상에서 널리 사용되는 다른 화학 처치 방법과 비교하는 것이다. 연구 재료 및 방법: 멸균된 거친 표면 티타늄 디스크에 S. aureus ATCC 25923을 접종한 후 48시간 동안 배양하여 바이오필름을 형성하였다. 실험은 대조군, 클로르헥시딘군(CHX), 테트라싸이클린군(TC), 인도시아닌 그린군(ICG), 인도시아닌 그린을 활용한 광역학치료군(ICG-PDT)으로 구분하여 진행하였다. 군에 따른 처리 후 세균을 배양하여 세균 수(colony forming unit; CFU)를 계산하고, 바이오필름을 공초점 현미경을 통해 분석하였다. 통계 분석은 CFU값을 로그값으로 변환한 후 분산 분석을 시행하였다. 결과: 모든 실험군은 대조군과 비교하여 유의한 항미생물 효과를 나타냈으며(P < 0.05), 특히 CHX, TC, PDT 군에서 90% 이상의 유의한 효과를 보였다(P < 0.01). CHX 군은 TC, PDT 군과 유의한 차이를 보이지 않았으나(P > 0.05), PDT 군은 TC보다 유의하게 효과적이었다(P = 0.035). 공초점현미경 상에서 PDT 군에서 사균의 비율이 더 높게 관찰되었다. 결론: 이번 연구를 통해 인도시아닌 그린을 활용한 광역학치료가 SLA 티타늄 표면에 형성된 S. aureus 바이오필름 제거에 있어 효과적임을 확인하였다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 국제심포지움
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• pp.59-72
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• 2002

The anticarcinogenic effects of epicatechin(EC) and ginsenoside Rb2(Rb2), which are major components of green tea and Korea ginsen, respectively, were investigated using a model system of gap junctional intercellular communication (GJIC) in WB-F344 rat liver epithelial cells. 12-O-Tetradecanoylphorbol-13-accetate (TPA) and hydrogen preoxide, known as cancer promoters, inhibited GJIC in the epithelial cells as determined by the scrape loading/dye transfer assay, fluorescence redistribution assay after photobleaching, and immunofluorescent staining of connexin 43 using a laser confocal microscope. The inhibition of GJIC by TPA and H2O2 was prevented with treatment of Rb2 or Ec. The effect of EC on GJIC was stronger in TPA-treated cells than in H2O2-treated cells, while the effect of Rb2 was opoosite to that of EC. EC, at the concentration of 27.8$\mu$g/ml, prevented the TPA-induced GJIC inhibition by about 60%. Rb2, at the concentration of 277$\mu$g/ml, recovered the H2O2-induced GJIC inhibition by about 60%. These results suggest that Rb2 and EC may prevent human cancers by preventing the down-regulation of GJIC during the cancer promotion phase and that the anticancer effect of green tea and Korea ginseng may come from the major respective conponents, EC and Rb2.

• 한국광학회지
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• 제31권3호
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• pp.142-147
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• 2020

본 연구에서는 Yb 광섬유 레이저 MOPA (master oscillator power amplifier) 시스템을 구축하여 고출력, 고효율의 근적외선 레이저 빔을 발진시키고, 이를 주기분극반전 준위상정합 비선형 광학 소자인 MgO:PPSLT에 단일 통과시키는 방식을 통하여 고출력 고효율 연속발진 녹색 레이저 빔을 생성하는 방법을 보고한다. 자발 펄싱을 억제할 수 있는 패브리-패롯 피드백 공진기 구조를 사용한 광섬유 레이저 주공진기를 사용하여 선폭이 좁고 선형 편광된 1064 nm 레이저 씨앗 빔을 안정적으로 생성할 수 있었으며, 이를 Yb 광섬유 증폭단에서 고출력으로 증폭시켰다. 증폭된 레이저 빔을 MgO:PPSLT에 통과시켜 고출력 고효율의 이차조화파를 얻을 수 있었는데, 이때 얻은 532 nm 레이저의 최고 출력은 기본 입사광의 출력이 25.0 W일 때 11.1 W였으며, 변환 효율은 44.4%를 얻었다.

• Journal of the korean society of oceanography
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• 제35권3호
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• pp.153-157
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• 2000

Four different FITC-conjugated lectins were used to visually evaluate lectin binding activity by optical staining quality using confocal laser scanning microscopy (CLSM) of Cochzodinium polykrikoides in nature (wild type) and culture (cultured type). Cells from the field and cultures treated with ConA fluoresced only at the outer cell wall, and the abundance and distribution of the fluorescent signal were similar. Treatment with PWM and HPA did not elicit fluorescence at the cell surface, but the wild type exposed to HPA showed greater binding than did the cultured cells, possibly due to greater concentrations of glucosamine. The wild type cells treated with LBL lectin showed a strong green fluorescence on the cell surface, whereas cultured cells did not. Signal intensity and abundance were greater than for any other lectins tested in this study. These results suggest that wild type and cultured type are significantly different based on surface sugar production. In particular, the wild type cells apear richer in galactosamine-like moieties. Neither glucose nor mannose-like moieties were present in either wild types or cultured cells.

• 한국진공학회:학술대회논문집
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• 한국진공학회 1996년도 제11회 학술발표회 논문개요집
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• pp.29-30
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• 1996