• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.469-472
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• 2003

A method that effectively precipitates capsular polysaccharide of Haemophilus influenzae type b (polyribosylribitol phosphate, PRP) conjugated to tetanus toxoid (TT), PRP TT in a liquid vaccine has been developed to measure free PRP present in TT-conjugate vaccine. The method involves adding anti-TT antibody and ammonium sulfate to precipitate PRP-TT conjugate and measuring free PRP in tile supernatant. This new method provides a complete precipitation of the total PRP-TT, and provides an accurate and reproducible measurements of free PRP. The accuracy of the assay was confirmed by spiking known amounts of unconjugated PRP to PRP-TT conjugate, and the new method was found to have no effect on free PRP while precipitating PRP-TT. The published acid precipitation method did not produce reproducible results due to incomplete precipitation of PRP-TT, especially when the vaccine is formulated in a salt-buffered solution.

• 한국정보디스플레이학회:학술대회논문집
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• 2007.08a
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• pp.911-914
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• 2007

We developed the laser annealing system using green laser of 261W(5kHz) and 75.5mJ/pulse(2kHz). We confirmed that this system makes it possible to form two kinds(large or uniformed grain) of poly-Si by changing its polarized directions. By using ${\mu}-crystal-Si$ as irradiated films, grain size uniformity is better than that using a-Si.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.111-111
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• 2003

Green tea have been widely reported as functional foods because of their various bioactivities. In the present study, we used 3T3-Ll cells model of white adipocytes to clarify whether green tea and its main pharmaceutically effective compounds (EGCG, caffeine and theanine) prevent obesity. Cellular viability, glycerol-3-phosphate dehydrogenase activity, glycerol release and HSL mRNA levels were checked. Glycerol release into the medium was significantly increased by the cells treated with green tea extract. Glycerol release into the medium was significantly increased by the cells treated with green tea extract. Caffeine and theanine from green tea showed some level of lipolytic activity, and glycerol-3-phosphate dehydrogenase activity was remarkably decreased by EGCG. These results suggest that green tea has anti-obesity effect through inhibition of adipogenesis and stimulation of lipolysis. Catechins and theanine of green tea might be the factors responsible for the modulation of lipid metabolism and adipocyte differentiation.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.130-135
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• 2000

The characteristics of two different modes of perfusion culture, intermittent and continuous bleedings, were investigated by culturing the hybridoma cells producing von Willebrand Factor (vWF) monoclonal antibody (McAb) in a 15 L bioreactor without clogging the filter. Both culture methods exhibited similar profiles of cell density and metabolite concentrations during the culture period at the cell concentration of around 1${\times}$107 cells/mL. When the perfusion rate was increased, the intermittrnt bleeding culture showed problems of ammonia accumulation and decrease of cell viability. The continuous bleeding culture in terms of nutrient consumption and metabolite production kinetics. But the analysis of specific oxygen consumption rate showed that the specific oxygen consumption rate of intermittent bleeding culture was similar to that of exponential growth phase. The continuous bleeding culture showed higher specific oxygen consumption rate of intermittent bleeding culture. finally we proved the possibility of long-term operation of continuous bleeding culture and produced approximately 40 g of vWF McAb in a 15L bioreactor after one-month operation.

• The Journal of Information Systems
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• v.25 no.1
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• pp.25-47
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• 2016

Purpose In this study, the relationships among environmental information exchange with suppliers(EIES), green collaboration with suppliers, green learning by environmental information, the adoption of green supply-chain management techniques(AGSMT) (i.e., the green purchasing, product design and production, and the recovery of extra facilities and inventories), environmental performance, and the organizational performance of a firm were empirically investigated. Design/methodology/approach Data for this study were drawn from a survey of the current status of EIES levels and AGSMT usage observed in Korean manufacturing firms. In total, 500 organizations were randomly selected from a population of about 1,000 firms that are listed on the Korean stock market. In order to collect empirical data, this research administered questionnaires with the sample firms. Only chief production executives were selected as respondents. A questionnaire with a cover letter was mailed to each respondent. The survey was conducted during a 3-month period between March 2015 and June 2015. Finally, 78 valid data of the sample firms were collected. Findings The results of this study showed that EIES positively affects the green corporation and learning. According to the results, it was observed that both green corporation and green learning have a positive impact on the levels of AGSMT(i.e., the green purchasing, product design and production). It was also demonstrated that AGSMT has a direct positive impact on environmental performance. In examining the effects of environmental performance on the organizational performance of a firm, significant impact was found.

• Bulletin of the Korean Chemical Society
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• v.32 no.spc8
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• pp.2938-2940
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• 2011

Novel C-aryl glucoside SGLT2 inhibitors containing triazine motif were designed and synthesized for biological evaluation. Among the compounds assayed, triazine containing methoxy moiety 18 demonstrated the best in vitro inhibitory activity against hSGLT2 in this series to date ($IC_{50}$ = 24.9 nM).

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.490-494
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• 2004

An efficacy test of PRP (polyribosylribitol phosphate)-TT (Tetanus toxoid) conjugate vaccines was carried out using BALB/c mice as an animal model by inoculating Haemophilus in­fluenzae type b (Hib) with a virulence enhancement factor (VEF). Three administrations of the conjugate vaccines at 2-week intervals elicited a significantly high level of PRP antibodies (P>0.0001). The protective activity of the PRP immunization was challenged with either Hib with iron dextran (Hib/) or with a combination of mucin and hemoglobin (Hibmh) as a VEF. The me­dium lethal dose $(LD_{50})$ for Hibmh and Hibiwas measured as 10 CFU (Colony Forming Unit) and $2.5{\times}10^{8}$ CFU respectively. Each immunized animal was challenged with five or ten times the $LD_{50}$ level of bacteria with a VEF. A significant difference in mortality between the immunized and control mice (P> 0.01) was observed with the Hibmh challenge inoculation but not with the Hibi challenge inoculation. These results show that a combination of mucin and hemoglobin was able to enhance the virulence of Hib in BALB/c mice to cause a lethal infection, thus suggesting that BALB/c mice introduced to this method can be an effective model animal for testing the protective efficacy of H. influenzae conjugate vaccines.

• Bulletin of the Korean Chemical Society
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• v.34 no.7
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• pp.2199-2202
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• 2013

• KEPCO Journal on Electric Power and Energy
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• v.7 no.1
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• pp.137-143
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• 2021

As an electrochemical water electrolysis for green hydrogen production, both polymer electrolyte membrane (PEM) and alkaline electrolyte are being developed extensively in various countries. The PEM electrolyzer with high current density (above 2 A/cm²) has the advantage of being able to design a simple structure. Also, it is known that it has high response to electrical output fluctuations. However, the cost problem of major components is the most important issue that a PEM electrolyzer must overcome. Instantly, there are platinum group metal (PGM)-based electrocatalysts, fluorine-based polyfluoro sulfuric acid (PFSA) membrane, Ti felt (porous transport layer, PTL) and so on. Another challenging issue is productivity. A securing outstanding productivity brings price benefits of the electrolytic cells. From this point of view, we conducted basic studies on manufacturing electrode and membrane electrode assembly (MEA) for PEM electrolyzer production.

• Korean Management Science Review
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• v.33 no.2
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• pp.11-28
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• 2016

This study empirically investigated the relationships among external factors (i.e., the imitation, compulsory and normative pressures, and governmental regulation), proactive environmental strategy, the adoption of green-supply chain management (GSCM) techniques, green corporation with suppliers, environmental performance, and organizational performance. To empirically demonstrate the relationships, 78 sample firms' data were collected from Korean manufacturing firms that are listed on the Korean stock market. The results of this study showed that a proactive environmental strategy mainly and positively influences the adoption of GSCM, and the introduction of a proactive environmental strategy is significantly and positively affected by governmental regulation. It was also found that governmental regulation has an indirect impact on the adoption of GSCM through the introduction of a proactive environmental strategy. Thus, it is asserted that governmental regulation, in Korean manufacturing firms, is the unique external factor on the adoption of a proactive environmental strategy, which facilitates the use of GSCM techniques. According to the results, it was observed that GSCM positively influences the levels of green corporation, and both GSCM and green corporation have positive effects on the improvement of environmental performance. Hence, it is suggested that the implementation of GSCM inevitably brings high degrees of green collaboration with suppliers. Finally, it was found that environmental performance has a significant and positive impact on the organizational performance of a firm. This result implies that high degrees of environmental performance, which bring both the efficient usage of materials and energy and the elimination of wastes, can lead to the increase of organizational performance.