• Title, Summary, Keyword: Grateloupia

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Grateloupia jejuensis (Halymeniales, Rhodophyta): a new species previously confused with G. elata and G. cornea in Korea

  • Kim, Su Yeon;Han, Eun Gyu;Kim, Myung Sook;Park, Jung Kwang;Boo, Sung Min
    • ALGAE
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    • v.28 no.3
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    • pp.233-240
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    • 2013
  • Despite specimens' large size and ease of collection in northeast Asian waters, the species diversity of the genus Grateloupia still needs more research in Korea. We investigated plastid rbcL sequences and carried out detailed morphological observation on flattened halymeniacean red alga collected in twelve locations around Korea and Japan. We describe Grateloupia jejuensis sp. nov. based on the distinct clade with high support in our rbcL tree. Grateloupia jejuensis is characterized by solitary or caespitose habit and flattened thalli with discoid holdfast, cartilaginous texture, and blunt or bifid axis. Grateloupia jejuensis was distantly related to G. elata and G. cornea, which have been morphologically confused with the former, and it formed a sister relationship with Prionitis filiformis from California, USA in the rbcL tree.

The Ecological Character and Sequence Similarity Analysis of 18S rDNA from the Two Species of Grateloupia (Grateloupia, Rhodophyta) in Korea (두 종의 한국산 홍조 지누아리류(Grateloupia filicina, Grateloupia divaricata)의 생태특성과 18S rDNA 염기서열 상동성 분석)

  • Kim, Young-Dae;Jin, Long-Guo;Song, Hong-In;Hong, Jung-Pyo;Lee, Chu;Jeon, Chang-Young;Kim, Su-Kyoung;Kim, Dong-Sam;Han, Hyoung-Kyun;Hong, Yong-Ki
    • Journal of Life Science
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    • v.16 no.5
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    • pp.723-728
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    • 2006
  • The species of Grateloupia filicina, Grateloupia divaricata in East Sea were investigated taxonmically in order to clarify taxonomic position. The ecological character, external morphology, anatomy of vegetative structure. Blade length are $15{\sim}40\;cm$, erect from discoidal holdfast of $3{\sim}10\;mm$ in diameter. Stipe $1{\sim}2.5\;cm$ long, narrowly cylindrical below, compressed above Grateloupia filicina. Main axis are long and compressed, $3{\sim}7\;mm$ broad in broadest part. Colors are scarlet to light red. Blade length are $10{\sim}25\;cm$, erect from discoidal holdfast of $3{\sim}8\;cm$ in diameter Grateloupia divaricata. Stipe are single and simple $2{\sim}5\;mm$ broad. Thallus composed of cortex and medulla in section ; cortex composed of $9{\sim}10$ layers of anticlinally arranged cortical cell, divided into outer, middle and inner parts. Partial fragments of nuclear 18S rDNAs from the two species of Grateloupia (Rhodophyta) were amplified using the PCR reaction and sequenced to compare their similarity. The partial sequences showed 98.9% similarity each other. Grateloupia filicina has 371 bp sizes and Grateloupia divaricata has 372 bp size. The G+C contents of Grateloupia filicina is 54.3% and Grateloupia divaricata is 53.64%.

Investigation of ${\alpha}$-Glucosidase Inhibitory Activity of Ethanolic Extracts from 19 Species of Marine Macroalgae in Korea

  • Jeong, So-Young;Qian, Zhong-Ji;Jin, Yeong-Jun;Kim, Gi-Ok;Yun, Pil-Yong;Cho, Tae-Oh
    • Natural Product Sciences
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    • v.18 no.2
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    • pp.130-136
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    • 2012
  • In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) f rom all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on ${\alpha}$-glucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity ($IC_{50}$, 2.17 ${\mu}g/ml$) against ${\alpha}$-glucosidase, followed by Sargassum fulvellum (<$IC_{50}$, 8.13 ${\mu}g/ml$), Dictyopteris prolifera ($IC_{50}$, 16.66 ${\mu}g/ml$), Rugulopteryx okamurae ($IC_{50}$, 50.63 ${\mu}g/ml$), and Petrospongium rugosum ($IC_{50}$, 101.62 ${\mu}g/ml$). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.

강원연안 홍조 지누아리사촌(Grateloupia acuminata, Rhodophyta)의 양식

  • 김형근;박중구;이해복
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • pp.269-270
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    • 2000
  • 홍조 지누아리류(Grateloupia spp.)는 독특한 향과 감촉을 가진 유용 해조류로서 강원도 지역에는 옛부터 식용으로 널리 이용되어 왔다. 그러나 산업적 생산량이 자연상태의 생물량에 한정되어 있어서 지역적 한계를 벗어나 그 우수한 풍미를 일반화시키지 못하고 있다. 본 연구에서는 강원도 연안에서 채집한 지누아리사촌(Grateloupia acuminata)의 사분포자체를 조직배양하여 재생체를 유도하고, 이로부터 사분포자를 얻어 배양조건에 따른 포자의 발아와 생장 경향을 파악하였다. 양식에 사용된 종묘는 사분포자로부터 얻은 배우체의 유엽으로서 이의 바다양성을 통하여 대량 양식의 가능성을 파악하고자 하였다. (중략)

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Molecular Classification of the Genus Grateloupia (Halymeniaceae, Rhodophyta) in Korea

  • Lee, Ju-Il;Kim, Hyung-Geun;Geraldino, Paul John L.;Hwang, Il-Ki;Boo, Sung-Min
    • ALGAE
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    • v.24 no.4
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    • pp.231-238
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    • 2009
  • The genus Grateloupia is the largest with more than 96 currently recognized species in the family Halymeniaceae, but morphological features of the genus are highly homoplasious making species identification difficult. In this study, we analyzed rbcL gene from 34 samples collected in Korea and compared the haplotypes of Korean species with those already published. The rbcL tree confirmed the occurrence of eight species in Korea: G. asiatica, G. catenata, G. divaricata, G. elliptica, G. imbricata, G. lanceolata, G. subpectina, and G. turuturu. All, except G. asiatica and G.catenata, been introduced from their native northeastern Asian waters to Australasian, European, and North American waters. The introduced haplotypes of G. lanceolata in Europe and North America were absent in Korea, while haplotype sharing of G. turuturu in Korea and Europe was common. Further sampling will show a source population of each introduced species and also provide a correct list of Grateloupia from Korea, including the other remaining 11 species of the genus.

Seasonal growth and reproductive status of Grateloupia subpectinata (Rhodophyta) on the east coast of Korea

  • Adharini, Ratih Ida;Kim, Joon;Kandasamy, Ganesan;Kim, Hyung Geun
    • Fisheries and aquatic sciences
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    • v.19 no.3
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    • pp.13.1-13.8
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    • 2016
  • Reproductive phenological pattern on tetrasporophytes and carposporophytes of Grateloupia subpectinata thalli were observed at Yangyang, on the middle east coast of Korea from January to December 2013. DNA sequences of cox1 gene from 48 selected samples were compared to assure that sample collection is single species, and they were all identical G. subpectinata. Density, length and width of thalli were significantly changed monthly. The maximum of density, length and fresh weight occurred in September, and continuous growth was observed during the autumn and winter. Tetrasporophytes were grown dominantly in October and November, whereas carposporophytes were dominated in September and March. Upright thalli disappeared in June and July and then juveniles were abundant in August. There was a significant difference in length between tetrasporophytes and carposporophytes. These reproductive pattern and seasonality together with phenological data of G. subpectinata would provide valuable information about successful seedling supply for aquaculture of the species in the future.

Structure and Reproduction of Grateloupia filicina (Halymeniaceae, Rhodophyta) from Indian Coast

  • Pooja Baweja;Dinabandhu Sahoo
    • ALGAE
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    • v.17 no.3
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    • pp.161-170
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    • 2002
  • The vegetative and reproductive features of Grateloupia filicina (Lamouroux) C. Agardh (Cryptonemiales, Halymeniaceae) from different parts of the Indian Coast were studied. The plants grow in wide range of habitats and showed a lot of morphological variations. The development of the thallus is multiaxial type and the medullary region is composed of irregular, branched and stellate shaped cells. The gametophytic plants are dioecious and the male plants are smaller compared to female plants. The carpogonial branch is two-celled and formed on an a accessory branch system known as ampulla. Cystocarps are spherical to sybspherical with distint ostioles and scattered on the thallus surface. Tetrasporangia are common and tetraspores are either cruciate or decussate. Bisporangia are occasionally encountered. Our study suggests occurrence of two intraspecific taxa of G. filicina i.e.: var. luxurians and var. filinina from India coast.

New records of three endophytic green algae from Grateloupia spp. (Rhodophyta) in Korea

  • Kim, Chansong;Kim, Young Sik;Choi, Han Gil;Nam, Ki Wan
    • ALGAE
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    • v.29 no.2
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    • pp.127-136
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    • 2014
  • Endophytic green algae growing in fronds of Grateloupia spp. were examined for infection frequency from their field populations of Jeju, Wando, and Uljin, Korea in August and September 2013. Three endophytes were isolated in laboratory culture from a G. lanceolata thallus collected in Jeju. Unialgal cultures were made from the endophytes, and their morphological characteristics were observed with light microscopy. In addition, a phylogenetic analysis based on chloroplast-encoded elongation factor tufA gene sequences was performed to identify the G. lanceolata endophytes. Three filamentous green endophytic species, Ulvella leptochaete, Blastophysa rhizopus, and Bolbocoleon piliferum were reported for the first time in Korea. General biological information for the three endophytes was also described.

Isolation of 6,6'-Bieckol from Grateloupia elliptica and its Antioxidative and Anti-Cholinesterase Activity

  • Lee, Bong Ho;Choi, Byoung Wook;Lee, Soo Young
    • Ocean and Polar Research
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    • v.39 no.1
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    • pp.45-49
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    • 2017
  • During the search for anticholinesterase compounds from marine organisms, we were able to isolate 6,6'-bieckol from a red alga, Grateloupia elliptica. This compound showed moderate acetylcholinesterase (AChE) inhibitory activity in a micromole range ($IC_{50}$ $44.5{\mu}M$). However, for butyrylcholinesterase (BuChE), a new target for the treatment of Alzheimer's disease (AD), it showed particularly potent inhibitory activity ($IC_{50}$ $27.4{\mu}M$), which is more potent compared to AChE. It also inhibits BACE-1, a new target for reducing the generation of ${\beta}-amyloid$.

Tissue Culture of Grateloupia acuminata (Rhodophyta) from the Eastern Coast of Koea (동해안 홍조 지누아리사촌(Grateloupia acuminata) 식물의 조직배양)

  • Kim Hyung-Geun;Park Joong-Goo
    • Journal of Aquaculture
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    • v.19 no.3
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    • pp.216-221
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    • 2006
  • The Effect of different temperatures and photon irradiance on the growth of crust and the regeneration of tissue fragments of the commercially important red alga Grateloupia acuminat Okamura were examined in laboratory cultures. The tetraspore developed into basal crusts and produced upright thalli. Crust grew very fast at $25^{\circ}C$ and $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after one week in indoor culture. However, they stopped growing after three weeks. Maximum growth was $275{\mu}m$ in diameter. They required four weeks to get upright thalli at $5^{\circ}C$, while only three weeks were required at $10^{\circ}C$. When different light intensities were compared at $15^{\circ}C$, cells of the crusts were well differentiated $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and consistently divided so that upright thalli were produced. In aeration culture, the upright thalli grew up to 6.5 cm in length within 4 months. Thus, it is possible to produce mass cultures of Grateloupia in the field. In addition, female and male gametophytes developed from the tetraspores and they were fertilized to produce tetrasporohyte thalli. By this procedure, the normal life cycle of the red alga G. acuminata was completed.