• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.819-827
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• 2017

Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). Results: All isolated strains (LCG3, LTG7, I5, and LI3) could grow at $5^{\circ}C$ to $20^{\circ}C$, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/total nitrogen (AN/TN) compared with uninoculated silages at various temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at $10^{\circ}C$ and $15^{\circ}C$, indicated by similar pH, LA content and AN/TN. Conclusion: All inoculants could improve the silage fermentation quality at various temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). At the temperature of $10^{\circ}C$ and $15^{\circ}C$, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1267-1272
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• 2005

The effect of various moisture contents of fresh forage on the change in nitrogen (N) fractions, in vitro ruminal N degradability, and the subsequent N utilization of silage in sheep were evaluated. Orchardgrass (Dactylis glomerata L.) with high (HM, 76%), medium (MM, 65%) and low (LM, 40%) moisture contents were ensiled into silos of 120 L capacity for 120 days. A nitrogen balance trial was conducted using a 4${\times}$4 Latin square design consisting of four dietary treatments (i.e. fresh forage, HM, MM and LM silages) and four wethers. With respect to N fractions, fraction 1 (buffer solution soluble N), fraction 2 (buffer solution insoluble N-neutral detergent insoluble N), fraction 3 (neutral detergent insoluble N-acid detergent insoluble N), and fraction 4 (acid detergent insoluble N) were determined. The proportion of fraction 1 in silages tended to decrease, while the in vitro ruminal degradability of insoluble N increased (p<0.05) with lower moisture contents at ensiling. Consequently, nitrogen utilization in sheep tended to improve as the moisture content of ensiled grass was decreased, with a negative correlation (p<0.01) between urinary N and the in vitro ruminal degradability of insoluble N. The averaged N retentions for HM, MM, and LM silage treatments were 59, 73 and 79% of that for fresh forage, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.11
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• pp.1542-1547
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• 2000

To standardize proper formulation of urea and molasses, the former to increase crude protein content of tropical grass and the latter for improving its silage quality, we examined the fermentation quality of silage of fresh and wilted napiergrass (Pennisetum purpureum Schumach) with different levels of urea and molasses with or without lactic acid bacteria (LAB). Silage was made of napiergrass with conditions of fresh young (Exp. 1),young wilted for half day (Exp. 2) and fresh mature (Exp. 3). Chopped plant materials of about 1cm length were ensiled into a laboratory silo and incubated for one month at $25^{\circ}C$. The treatments were the combination of 0, 0.2 and 0.6% of urea and 0, 2 and 5% of molasses (fresh material basis) with or without LAB inoculation. After opening the silo, pH, organic acids, volatile basic nitrogen (VBN) and total nitrogen (TN) were determined. Addition of molasses significantly (p<0.01) lowered pH values in three experiments. Though molasses addition increased lactic acid production even at a higher level of urea, pH values at 0 and 2% molasses were significantly increased by urea in fresh and wilted young silages, but in fresh mature silage it occurred only when molasses was not added. VBN/TN at 0.6% urea were decreased significantly by the highest molasses in three experiments. Significant increases in TN by the increasing of urea addition were observed at all levels of molasses in wilted young and fresh mature silages. In conclusion, a combination of 5% molasses and 0.6% urea could improve the nutritive and fermentation qualities of napiergrass silage under young, wilting and mature conditions.

• Journal of Practical Agriculture & Fisheries Research
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• v.14 no.1
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• pp.61-83
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• 2012

To identification of fungi that occurs round bale silages, 253 fungal contaminated samples were collected from 2009 to 2011. Total 253 silage samples from Italian ryegrass, sudan grass, rye, corn, barley and oat were analysed. Total 270 strains were purely isolated from contaminated round bale silages. The fungi were identified with morphological characteristics and rDNA sequence analysis. Nineteen species of fungi(Rhizopus sp., Fusarium spp., Coprinus sp., Blastomyces sp., Aureobasidium sp., Polypaecilum sp., Botryoderma sp., Mucor sp., Scytalidium sp., Sphaeropsis sp., Aspergillus spp., Trichocladium sp., Humicola sp., Staphylotrichum sp., Periconia sp., Verticillium sp., Diplococcium sp., Penicillium spp. and Trichoderma spp.) were identified by morphological characteristics. On the other hand, fungi isolated from silage were identified to Acremonium strictum, Aspergillus tubingensis, Bionectria ochroleuca, Dipodascaceae sp., Fusarium proliferatum, Fusarium oxysporum, Fusrium solani, Gelasinospora reticulata, Gibberella moniliformis, Gibberella zeae, Nectria mauritiicola, Penicillium paneum, Pseudallecheria boydii, Schizophyllum commune, Scopulariopsis brevicaulis and Simplicillium lamellicola by rDNA sequence analysis. Penicillium sp. and Trichoderma sp., were isolated 74 and 64 strains, respectively. Humicola sp., Aspergillus sp., Coprinus sp., and Fusarium spp. were identified 10 to 30 strains. Most fungi were isolated together with more than one species in a sample looked like one species with the naked eyes.

• Korean Journal of Agricultural Science
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• v.38 no.3
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• pp.437-444
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• 2011

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The experimental trials were conducted at Unbong Animal Genetic Resources Station in 2008. The experimental roughages include five sources and 25 species in all; grasses and legumes: 5 species (mixed grasses, orchardgrass, tall fescue, alfalfa, white clover), native grasses and weeds: 5 species (mixed native grasses, Miscanthus sinensis Anderss, Arundinella hirta (Thunb.) Tanaka, barnyard grass, short awn, forage crops (hay, silages and straw): 5 species (barley + hairy vetch, Wheat + hairy vetch, rye silage, barley silage, baled rice straw), browse and fallen leaves: 5 species (mixed browse, oriental white oak, Quercus serrta Thunb., oriental cherry fallen leaves, Japanese chestnut fallen leaves), and imported hays and straws: 5 species (timothy hay, tall fescue straw, annual ryegrass straw, klinegrass hay, alfalfa hay). Five sika deer were used as experimental animals and the averaged body weight was 95+5.4kg. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period(p<0.05). The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake roughage sources ranking by sika deer was observed like this: browse and fallen leaves (32.2%), grass and legumes (27.0%), native grasses and weeds (22.0%), imported hays (12.9%) and forages crops (5.5%) respectively. Although, the sika deer ate more browse leaves, but ate more roughage which had low fibrous contents (NDF and ADF), but high drymatter digestibility. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse and fallen leaves (32.2%). Based on the result, the food habit of sika deer seems to be closer to the typical browser.

• Korean Journal of Organic Agriculture
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• v.11 no.4
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• pp.75-92
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• 2003

Organic livestock farming in northern European regions has been expanded with the major animals of large ruminants using pastures and grass silages. Organic livestock farming in some European countries has been in rather short of productivity compared to the conventional livestock farming, however since the gap of productivity between organic and conventional livestock farming has been reported to be reduced when the efficiency of management would improve, organic livestock farming has a potential to develop as a clean livestock farming in the future. We expect that organic livestock farming be propelled to a future model of livestock farming in Korea too. As the schemes for realization of organic livestock farming in Korea, firstly a system for the consistent supply of organic feed should be established. Mountainous areas that represents 63 % of total area of Korea could be utilized for the production of organic forages. Uncultivated rice paddy and upland agricultural field could also be used for this purpose. The active application of organic agricultural by-products such as organic rice straw, organic rice bran and SO forth can be considered for organic livestock farming. Secondly, the replacement of anti-biotics for the management of animal diseases should be developed using natural products. Plants and microbes would be good sources of natural products. Thirdly, the realization of organic livestock farming may require a system for certification of the organic farms and consequently the experts to work on.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.3
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• pp.133-138
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• 2006

This experiment was conducted to determine the effect of wrap color on the quality of round baled grass silage at experimental field of Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three wrap color(white, black and light green). Wrap color did not affect chemical composition. Fiber components(ADF and NDF) of all silages after 2 months were higher than those of forages at ensiling. Among tested wrap colors, white color wrap resulted in lower pH than others (p<0.05) but, there was no significant difference between black and light green color. Dry matter content of light green color was the highest among warp colors, but there was no signifiant difference (p<0.05). Acetic and butyric acid contents of all plots. were not found significant difference among wrap color and wrap color did not influence lactic acid and organic acid concentration. The effect of wrap color on the quality grade and DM loss also were not found significant difference. Results of this study indicate that wrap color does not influence the quality of silage.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1701-1704
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• 2001

The evaluation of structural carbohydrate losses and its effect on silages digestibility in alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.) was studied during 5, 21 and 56 days ensiling. About 70 and 60 kg fresh matter of the two forages were ensiled in 9 silos of 120 L capacity. The digestion trials were conducted in two phases using the two grasses in two $4{\times}4$ Latin square design according to the four treatments being the grass and the three silages. There were no differences in the DM and CP contents resulting from 5 to 56 days ensiling in both forages. The water-soluble carbohydrates (WSC), hemicelllose, pectin, and energy were slightly reduced and appeared lower in 56 days silage. The ether extract and cellulose contents slightly increased as the ensiling process advanced in the two species. Hemicellulose losses of 29 and 41 g/kg DM were obtained in alfalfa and orchardgrass, respectively, 56 days after ensiling. While the cellulose losses in both species were very little, compared to that for hemicellulose, the pectin losses, 56 days after ensiling were 15 and 12 g/kg DM in alfalfa and orchardgras respectively. The total structural carbohydrates lost (ie., hemicellulose + cellulose + pectin) in g/kg DM of fresh material forage ensiled, is about four fifths the amount lost by WSC, in alfalfa and about two thirds, in orchardgrass, by 21 days ensiling after the activity of microorganism terminated, indicating that appreciable amount was used as substrate for silage fermentation. Ensiling alfalfa and orchardgrass for 0, 5, 21 and 56 days maintained a decreasing trend of 83.8, 82.5, 79.3 and 78.9% digestibility in alfalfa and 80.5, 77.0, 77.1 and 76.4% digestibility in orchardgrass. While the digestibility of cellulose and ether extract increased in silage in both species, the digestible energy values in silage were reduced from 2.6 to 2.3 and 2.9 to 2.7 Mcal/kg DM respectively in alfalfa and orchard during 5-56 days ensiling.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.5
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• pp.636-642
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• 2011

The effect of different rates of ethanol additive on fermentation quality of napiergrass (Pennisetum purpureum) and residual water soluble carbohydrate were studied in the experiment. The addition rate of ethanol was 0%, 1.5%, 2.5%, 3.5%, 4.5% on fresh weight of napiergrass. The laboratory silos were kept in the room, then were opened on 1, 3, 5, 7, 14, 30 days after ensiling and the changes of silage quality were analyzed, respectively. There was a fast and large reduction in pH from the 5th day of ensiling to below 4.2 except for the 4.5% treatment. After five days the pH of silage decreased slowly and the pH of the ethanol additions was lower than the control. Lactic acid content of ethanol treatments increased significantly (p<0.05) from the 5th day of ensiling, reaching the highest value on either the 7th day or 14th day. The ethanol additive inhibited the break down of silage protein and the ammonia nitrogen content of ethanol addition silage was significantly (p<0.05) lower than the control after 30 days of ensiling. Within the initial first day of ensiling the water soluble carbohydrate content declined quickly. The efficiency of water soluble carbohydrate usage was higher in silage with ethanol than in the control. The acetic acid of ethanol treatment was significantly (p<0.05) lower than control on first and 14th day, but there was no significant (p>0.05) difference among the ethanol addition silages. The volatile fatty acids content of silage increased gradually from the first day of ensiling and reached the peak on 14th day or 30th day and the content of ethanol addition treatment was significantly (p<0.05) lower than the control. The experimental results indicated that adding ethanol inhibited the use of protein and water soluble carbohydrate of aerobic bacteria and reduced the silage losses during the early stage of ensiling and thus supplied more fermentation substrate for lactic acid bacteria and improved the fermentation quality of napiergrass.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1123-1132
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• 2015

Winter wheat is a suitable crop to be ensiled for animal feed and China has the largest planting area of this crop in the world. During the ensiling process, lactic acid bacteria (LAB) play the most important role in the fermentation. We investigated the natural population of LAB in whole-crop wheat (WCW) and examined the quality of whole-crop wheat silage (WCWS) with and without LAB inoculants. Two Lactobacillus plantarum subsp. plantarum strains, Zhengzhou University 1 (ZZU 1) selected from corn and forage and grass 1 (FG 1) from a commercial inoculant, were used as additives. The silages inoculated with LAB strains (ZZU 1 and FG 1) were better preserved than the control, with lower pH values (3.5 and 3.6, respectively) (p<0.05) and higher contents of lactic acid (37.5 and 34.0 g/kg of fresh matter (FM), respectively) (p<0.05) than the control. Sixty LAB strains were isolated from fresh material and WCWS without any LAB inoculation. These LAB strains were divided into the following four genera and six species based on their phenotypic, biochemical and phylogenetic characteristics: Leuconostoc pseudomesenteroides, Leuconostoc citreum, Weissella cibaria, Lactococcus lactis subsp. lactis, Lactobacillus buchneri, and Lactobacillus plantarum subsp. plantarum. However, the prevalent LAB, which was predominantly heterofermentative (66.7%), consisted of Leuconostoc pseudomesenteroides, Leuconostoc citreum, Weissella cibaria, and Lactobacillus buchneri. This study revealed that most of isolated LAB strains from control WCWS were heterofermentative and could not grow well at low pH condition; the selective inoculants of Lactobacillus strains, especially ZZU 1, could improve WCWS quality significantly.