• Title/Summary/Keyword: Grass Lipid

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Effects of Grass Lipid and Its Fatty Acids on Ruminal Fermentation and Microbial Growth In Vitro

  • Yang, U.M.;Fujita, H.;Chung, T.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.2
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    • pp.176-181
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    • 2000
  • In order to clarify the inhibitory effects of orchardgrass (Dactylis glomerata L.) lipids on ruminal fermentation and digestion, two experiments were carried out in vitro. Experiment 1 was carried out using residues of grass hay from which the lipid fraction was removed by ether extraction. To ground grass samples were added 0, 1.5, 3.0, 4.5 and 6.0% lipids and incubated anaerobically at $39^{\circ}C$ for 24 h, with the mixtures of artificial saliva and rumen fluid. Increasing grass lipid levels remarkably reduced DM and NDF disappearances. Volatile fatty acid concentration was significantly reduced at 3.0, 4.5 and 6.0% lipid levels. Microbial nitrogen proportion to total nitrogen tended to decrease by the addition of the lipids. These results indicated that grass lipids have a marked inhibitory effect on ruminal fermentation and digestion, especially when to the substrate was added 3% or more grass lipids as ether extracts. Experiment 2 was conducted to study the relationship between changes in the free fatty acids and changes in the fermentation traits. Samples were incubated for 3, 6, 9, 12, 15, 18, 21 and 24 h as a sole substrate. The polyunsaturated fatty acids steadily decreased during incubation, whereas the saturated fatty acid ($C_{18:0}$) increased. It was suggested that the hydrogenation was extended during the initial stage of incubation. The unsaturated fatty acids ($C_{18:2}$, $C_{18:3}$) produced at the initial stage of incubation were negatively correlated with the amount of microbial N and DM disappearance, indicating that polyunsaturated fatty acids had the possibility to show an inhibiting effect on ruminal fermentation and digestion.

Influence of Salinity Treatment on Seed Germination and Polyamine Synthesis in Barnyard Grass(Echinochloa hispidula) (강피종자의 발아와 폴리아민 생합성에 대한 염류의 영향)

  • Yun, Sol;Lee, Su-Yeon;Lim, Hyo-Jin;Shim, Myoung-Bo;Sung, Jwa-Kyung;Kim, Tae-Wan
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.1
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    • pp.19-24
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    • 2004
  • To illuminate the physiological response to salinity, barnyard grass (Echinochloa hispidula) was germinated with high concentration of NaCl and KCL. Duration and promptness of seed germination were observed. Under salt stress, lipid peroxidation and polyamine biosynthesis were also analyzed. It appeared that high salt treatments per se did not provoke an inhibition of germination although the process of germination was significantly delayed. In context of lipid peroxidation and polyamine biosynthesis, we would imply that barnyard grass is tolerant to salinity. The increase in lipid peroxidation and putrescine content was prolonged only for 1 day after saline treatment. It could be concluded that these early acciimulation of putrescine and production of lipid peroxide seems to be associated with salt tolerance in the short-term. The physiological interest of these responses was discussed.