• Asian-Australasian Journal of Animal Sciences
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• v.17 no.1
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• pp.53-62
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• 2004

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

• Animal Bioscience
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• v.36 no.2
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• pp.238-247
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• 2023

Objective: Recently, indigenous Korean grass Sasa quelpaertensis Nakai (SQ) has garnered much interest as a roughage source for livestock to mitigate its adverse effects on habitat diversity. Thus, the objective of the present study was to evaluate the ruminal fermentation, palatability, and nutrient digestibility of SQ for Korean native beef cattle (Hanwoo) using in vitro rumen fermentation, in situ rumen degradability, and in vivo feeding trials. Methods: Using in vitro tests with rumen fluid as the inoculum for 48 h, ruminal fermentation of SQ was evaluated and compared with that of other roughage sources commonly used in Korea (i.e., rice straw, Timothy hay, and Italian ryegrass [IRG]). Additionally, an in situ trial 96 h was performed using three cannulated Hanwoo steers. Further, an in vivo trial was performed using eight Hanwoo steers to compare the palatability of SQ with rice straw in total mixed ration (TMR) and forage-concentrate separate feeding conditions. Finally, an in vivo digestibility trial of SQ fed as TMR of two particle sizes was performed with four Hanwoo steers. Results: In vitro and in situ trials revealed that SQ was comparable or superior to rice straw in terms of the ruminal fermentation characteristics of pH, gas production, total volatile fatty acid content, and effective ruminal dry matter digestibility (DMD), although its fermentability was lower than that of Timothy hay and IRG. In the palatability test, steers showed a greater preference for SQ when given as TMR. The total tract DMD of SQ fed as TMR was 75.9%±1.37%, and it did not differ by particle size. Conclusion: The feed value of SQ as a roughage source for Hanwoo steers is comparable or superior to that of rice straw, particularly when provided as TMR.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.7
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• pp.940-945
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• 2011

In order to determine whether oxalate from grasses affects feed intake, blood calcium (Ca) and other blood parameters of adult sheep, two feeding trials were conducted. In Trial 1, one group of sheep received guineagrass (0.47% soluble oxalate) and another group received setaria (1.34% soluble oxalate) for 28 d. In Trial 2, one group of sheep received guineagrass while another group received the same grass treated with an oxalic acid solution (at a rate of 30 g oxalic acid/kg dry matter of hay) for 72 d. All sheep received concentrate mixtures (0.5% of body weight) throughout the experiment. In both trials, it was observed that plasma Ca concentration (11.0-11.7 mg/dl) was significantly (p<0.05) lower in sheep fed high oxalate-containing grasses than in sheep fed low oxalate-containing grasses (12.4-13.7 mg/dl). No differences (p>0.05) were observed in concentrations of magnesium, phosphorus and parathyroid hormone in plasma between the feeding of low and high oxalate-containing grasses. In addition, no differences (p>0.05) were observed in roughage dry mater (DM) intake, total DM intake or body weight of sheep. This study suggests that sheep may consume oxalate-rich forage, but Ca bioavailability may decrease with increasing oxalate levels in the ration.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.344-350
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• 2011

Kura clover (Trifolium ambiguum M. Bieb.) is a potentially useful perennial legume because of its excellent nutritive value and persistence under environmental extremes. However, information about forage quality of kura clover - grass mixtures adapted to the North-Central USA is limited. Objectives of this research were to determine forage nutritional value of kura clover-grass mixtures under different harvest frequency and cutting height regimes. 'Rhizo' kura clover was grown alone and in binary mixtures with 'Park' Kentucky bluegrass (Poa pratensis L.), 'Comet' orchardgrass (Dactylis glomerata L.), and 'Badger' smooth bromegrass (Bromus inermis Leyss.) at the Arlington Agricultural Research Station located near Madison, WI. Three harvest frequencies ($3{\times}$, $4{\times}$, or $5{\times}$ annually) and two cutting heights (4- or 10-cm) were imposed on each binary mixture and on kura clover grown alone. Higher nutritive value was observed in the binary mixtures with more frequent harvest and lower cutting height. Averaged over 3 years and all harvest frequency and cutting height treatments, the nutritive value of the Kentucky bluegrass and smooth bromegrass mixtures was superior to that of the orchardgrass mixture ($410\;g\;kg^{-1}$ NDF and $194\;g\;kg^{-1}$ CP in the Kentucky bluegrass mixture; $405\;g\;kg^{-1}$ NDF and $188\;g\;kg^{-1}$ CP in the smooth bromegrass mixture; $435\;g\;kg^{-1}$ NDF and $175\;g\;kg^{-1}$ CP in the orchardgrass mixture). All of the mixtures and harvest management systems evaluated in this study produced forage with quality equivalent to "grade one" alfalfa hay and suitable for highproducing livestock, even though the highest quality was observed in the Kentucky bluegrass mixture with $5{\times}$ harvesting at the shorter cutting height.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.118-123
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• 1995

This experiment was canied out to determine the effects of nitrogen (N) fertilization level (0, 70, 140, 280 and 420 k a a ) on the grass growth, dry matter (DM) production, numtive value, and botanical composition in reed canarygrass (Phalaris ancndinacea L.) pasture. \ulcornerhe cultivar used in this study was Palaton, and the grass was harvested at soiling-hay stage (3 times per year) in 1992 and 1993. As the N level was increased, the plant height, DM produdion, and crude protein (CP) content were significantly increased (P<0.05). When the N was applied at the levels of 0, 70, 140, 280, 420 kg, DM yields were 4,540, 5,316, 6,060, 7,923 and 9,960 kgha, and CP contents were 17.6, 17.4, 17.4, 18.4 and 19.8%, respectively. However, as the N level was increased, DM percentage, total digestible nutrients, and relative feed value were decreased, while the contents of neutral detergent fiber and acid detergent fiber, and bareland were continuously increased, particularly in the plot of N 420 kg fdzation. From the above results, it is suggested that reed canarygrass was one of the sensitive pasture grasses to N fertilizer, and the proper amount of N application was 280 kg/ha/yr for desirable production, nutritive value, and botanical composition in r e d conarygrass pasmre.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1262-1275
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• 2013

Awamori is produced by fermenting steamed indica rice. Awamori-pressed lees is a by-product of the Awamori production process. Tofu lees is a by-product of the Tofu production process. Research was conducted to test if dried Awamori-pressed lees and Tofu lees can be used as a mixed feed ingredient for raising male goats. Eighteen male kids were divided into three groups of six animals (control feed group (CFG), Awamori-pressed lees mixed feed group (AMFG), Tofu lees mixed feed group (TMFG)). The CFG used feed containing 20% soybean meal as the main protein source, while the AMFG and TMFG used feed mixed with 20% dried Awamori-pressed lees or dried Tofu lees. The groups were fed mixed feed (volume to provide 100 g/d increase in body weight) and alfalfa hay cubes (2.0 kg/d) twice a day (10:00, 16:00). Klein grass hay and water was given ad libitum. Hay intake was measured at 10:00 and 16:00. Body weight and size measurements were taken once a month. At the end of the experiment, a blood sample was drawn from the jugular vein of each animal and the carcass characteristics, the physical and chemical characteristics of loin were analyzed. DCP and TDN intakes in AMFG and TMFG showed no significant difference to the CFG. Cumulative measurements of growth in body weight and size over the 10 mo period in the AMFG and TMFG were similar to the CFG. Blood parameter values were similar to those in normal goats. Dressing carcass weight and percentages, and total weight of meat in the AMFG were similar to that in the CFG, but smaller in the TMFG. The compressed meat juice ratio was higher in both the TMFG and AMFG than the CFG. While the fat in corn, Awamori-pressed lees, and Tofu lees contains more than 50% linoleic acid, the loin fat in both the AMFG and TMFG was very low in linoleic acid due to the increase in the content of oleic acid, stearic acid, and palmitic acid. This indicates that feeding on AMF and TMF does not inhibit hydrogenation by ruminal microorganisms. As in the CFG, the total essential and non-essential amino acids in the loin of the AMFG and TMFG were well balanced. Compared to the CFG, the AMFG and TMFG were high in taurine and carnosine. The results indicate dried Awamori-pressed lees and Tofu lees can be used as a feed ingredient for raising male goats.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.719-726
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• 2002

Nutritional adequacy of growing Thoroughbred horses raised in an alternate feeding system - grazing during late spring through late fall and stable feeding for the rest of seasons - was assessed by determining vitamin E and trace mineral levels in the serum and blood chemistry related to nutrition and health. During the stable feeding in winter and early spring, 50 growing female horses were fed concentrates (1.4% of their body weight), grass hay (0.62%) and alfalfa hay (0.37%). For the grazing period, the same horses were fed supplementary concentrates (1.1%) during late spring through early summer, and concentrates (1.1%) and alfalfa hay (0.5%) during late summer through late fall. Blood samples were collected before grazing in early spring, and during grazing in early summer through late fall. Serum vitamin E, BUN, GTP, total bilirubin and direct bilirubin levels were increased (P<0.01) by grazing compared to those measured before the initiation of grazing. Horses had lower (P<0.01) serum Fe contents in early summer than in late fall or in time of stable feeding. Stable feeding increased (P<0.01) serum Cu content compared to grazing in both early summer and late fall. In late fall, serum Zn level increased (P<0.01) compared to that found in the other seasons. Blood glucose and creatinine levels decreased (P<0.01) after grazing. Results indicate that supplementations of some minerals and vitamin E are not always necessary in diets for growing horses and should be done after careful evaluation of diets with regard to concentrations and biological availability of minerals.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.210-214
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• 1997

Ruminal degradation characteristics and intestinal availability of crude protein (CP) in four animal-origin feeds (fish meal, meat meal, viscera meal, feather meal) were estimated by mobile nylon bag technique. Three ruminally and duodenally cannulated Holstein dairy cows (average body wt. 550kg) fed a diet containing 40% concentrate and 60% orchard grass hay on a dry matter (DM) basis. Assuming that the outflow rate of diet in rumen is 5% per hour (k =0.05), contents of quickly degradable CP (QDP), slowly degradable CP (SDP), and undegradable CP (UDP) in the rumen were 27.6%, 9.4%, 63.0% for fish meal, 34.3% 28.1%, 37,6% for meat meal, 43.9%, 12.5%, 43.6% for viscera meal, and 14.4%, 15.8%, 69.8% for feather meal, respectively. Intestinal CP degradability was 51.0% for fish meal, 27.2% for meat meal, 37.9% for viscera meal and 56.2% for feather meal. Available UDP in the intestinal tract was contained 288 g, 217 g, 246 g and 423 g per kilogram DM of diet in fish meal, meat meal, viscera meal and feather meal, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.679-683
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• 2006

A feeding trial was conducted with 10 sheep for 12 weeks to examine the effect of soybean oil (SBO) supplementation on long-chain fatty acids composition, especially cis9,trans11-conjugated linoleic acid (c9,t11-CLA) in fat tissues. Sheep were fed either a SBO supplemented diet (5%, DM basis) or a control diet without SBO. Chopped rye grass hay was fed as roughage. Concomitant increases in contents of total cholesterol (T-C) and HDL-cholesterol (HDL-C) in the plasma of sheep were observed from the SBO supplementation. The supplementation of SBO reduced (p<0.05) the proportions of $C_{16:1}$, $C_{17:0}$ and $C_{17:1}$ but increased (p<0.05) the proportions of $C_{18:0}$ and octadecenoic acid (t11-$C_{18:1}$) in the intramuscular fat. The $C_{18:0}$ proportion only in the subcutaneous fat was increased (p<0.05) by the SBO supplementation. The SBO supplementation slightly increased CLA proportion in the intramuscular fat and subcutaneous fat.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.378-384
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• 1997

The ruminal solubility of copper (Cu), manganese (Mn), molybdenum (Mo), and zinc (Zn) in eight Philippine forages was studied. These forages were: 1) grasses: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), and napiergrass (Pennisetum purpureum Schumach.); 2) creeping legumes: calopogonium or calopo (Calopogonium muconoides Desv.) and centrocema (Centrocema pubescens Benth.); 3) tree lequmes: gliricidia or kakawate (Gliricidia sepium (Jacq.) Walp.), leucaena or ipil-ipil (Leucaena leucocephala (Lam.) de Wit.), and sesbania or katuray (Sesbania grandiflora (L.) Poir). Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72h in three rumen cannulated sheep fed with timothy hay (Phleum pratense L.) at 2% body weight/d. The 0-h bags were washed with deionized water. There were species differences (p < 0.05) on the different solubilities of trace elements. At O-h incubation, the trend of solubility was Mo (54%) > Zn (43%) > Cu (38.7%) > Mn (29.5%). At 3-h incubation, all the elements except Mn had an average solubility above 50%. Combining particulate passage rate (1.9%/h) and various trace elements disappearance rates, the effective ruminal solubilization (ERS) of trace elements were computed. The ERS across species ranged from 44.6 to 89.9% for Cu, 29.9 to 84% for Mn, 66 to 95.1% for Mo, and 30.1 to 82.3% for Zn resulting to a trend of Mo > Zn > Cu > Mn.