• 대한세포병리학회지
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• 제14권1호
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• pp.17-21
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• 2003

Insular carcinoma of the thyroid(ICT) is an uncommon thyroglobulin-producing neoplasm, intermediate between well differentiated and anaplastic carcinoma. Only a few publications have addressed the fine needle aspiration cytologic(FNAC) findings from ICT. We experienced a case histologically diagnosed as ICT and with preoperative FNAC in a 52 - year - old woman. The FNAC displayed scanty colloid and abundant monomorphic follicular cells presented singly, in small loose aggregates, and in cohesive trabecular or acinar clusters. Intact insulae of tumor cells were also identified. Necrosis and mitosis were rare. Tumor cells showed round and monomorphic nuclei, finely granular chromatin, and inconspicuous nucleoli. When insular structure is identified in thyroid FNAC specimen, ICT should be included in the differential diagnosis. Herein we discuss and review the cytologic criteria for separation of ICT from other thyroid neoplasms.

• Applied Microscopy
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• 제28권3호
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• pp.329-343
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• 1998

Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

• 사상체질의학회지
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• 제14권1호
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• pp.100-111
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• 2002

1. The Purpose of study An experimental study has done to examine the effect of defense on gastric mucosal damage of Jowesungcheong-tang. 2. The Material and Method of study Mice had intragastric injected with JST extract before indome thacin treatment which induces hemorrh age erosion artificially. General morphology, infiltrative cell in mucosa, the distribution of UEA-I, COX-1, MAC-1. ICAM, and Apoptotic cell were objected (Ahhreviation) JST :Jowesungcheong-tang, UEA-I : ulex europaeus agglutinin-I, COX-1: cyclooxyhenase-1, ICAM : intercellular adhesion molecule-1, GPE : Gastropathy elicitated mice 3. The results and Conclusions of study 1) The degree of hemorrhage erosion in GPE-group had increased conspicuously in gastric gland proper. JST -group were the same as normal 2) The noticeable increase of granular lecocytes and lymphocytes in GEP-group were seen, but in JST group, the configuration is decreased 3) The decrease of UEA-I positive reacted cells, COX-1, surface epithelial cells and the increase of MAC-l positive cells, ICAM-l positive cells had shown in GPE-group, but in JST-group UEA-I positive cells, COX-1 surface epithelial cells were in creased and MAC-1 positive cells, ICAM-l positive cells were decreased than GPE-group. 4) A number of apoptotic cells were distributed in hemorrhage erosion. The remarkable decrease of apoptotic cells were shown in JST-group.

• 한국패류학회지
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• 제4권1호
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• pp.30-41
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• 1988

The gonadal development, the annual reproductive cycle and the first sexual maturity of surf clam, Mactra veneriformis Reeve were studied histologically. Speciemens were monthly collected at the intertidal zone of Naechodo, Chollabuk-do, Korea, for one year from March 1986 to February 1987. Sexuality of the clam is dioecious. The gonads were located between the subregion of mid-intestinal gland in the visceral cavity and the reticular connetive tissues of the foot, The ovary is composed of a number of ovarian sacs, and the testis comprise several testiculat lobules. The undifferentiated mesenchymal tissues and eosinophilic granular cells function as nutritive cells in the early stage. The ripe eggs were about 50-60${\mu}{\textrm}{m}$ in diameter, and they were wurroundedby the gelatinous membranes. The spawing period was from early June to September the main spawning occurred beetween July and August when the water temperature reached above 24$^{\circ}C$. The annual reproductive cycle of this species could be classified into five successive stages: multiplicative(January to March), growing(March to May), mature(April to August), spent(June to September), degenerative and resting(September to February). The monthly changes of fatness coefficient closely correlated with the annual reproductive cycle. Percentages of the first sexual maturity of female and male clams were over 50% among those individals ranging from 2.1 To 2.5cm, and 100% in those over 2.6cm in shell length.

• 대한세포병리학회지
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• 제17권1호
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• pp.56-62
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• 2006

Salivary duct carcinoma (SDC) is a rare primary salivary gland malignancy characterized by histological features similar to those of ductal carcinomas of the breast. It is regarded as a high-grade malignancy associated with frequent local recurrences and early distant metastases that require aggressive treatment. The typical fine needle aspiration cytology (FNAC) findings in SDC include cellular smears showing tumor cells with eccentric pleomorphic nuclei and a granular cytoplasm arranged in flat sheets or cribriform patterns against a necrotic background. However, the presence of mucin-containing cells in SDC has been rarely described. We report the FNAC findings in a patient with histologically confirmed SDC that demonstrated numerous mucin-containing cells and was subsequently misdiagnosed as a high-grade mucoepidermoid carcinoma. Here we discuss the problems involved in distinguishing SDC from high-grade mucoepidermoid carcinoma on the basis of cytologic findings alone.

• 한국동물학회지
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• 제21권1호
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• pp.29-39
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• 1978

양서류 피부 점액선의 선상피세포의 미세구조를 관찰하기 위하여 양서류 피부조직을 2.5% glutaraldehyde-paraformaldehyde(pH7.2) 1% osmium tetroxide에 전후 고정 한후 ethanol과 aceton으로 탈수, Epon 812에 포매 Sorvall MT-2B ultramicrotome으로 ultrathin section을 만들어 uranyl acetate와 lead citrate으로 염색하여 JEOL-100B형 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 양서류 피부 점액선은 선상피세포와 근상피세포로 이루어졌다. 2. 양서류 피부 점액선의 선상피세포는 미세구조적인 면에서 종에 따라 여러형의 세포들이 관찰되었다. a. 도롱뇽에서는 암세포와 명세포가 관찰되었다. b. 무당개구리에서는 mitochondria가 많은 세포와 원형의 분비과립을 갖는 세포들이 관찰되었다. c. 맹꽁이에서는 구형의 분비과립을 갖는 세포와 거품모양의 과립괴를 갖는 세포들이 관찰되었다. d. 개구리 피부 점액선은 A형과 B형점액선으로 구분되었고, A형점액선 상피에서는 mitochondria가 많은 세포와 원형의 분비과립을 갖는 세포, B형점액선 상피에서는 mitochondria가 많은 세포와 많은 분비과립을 갖는 세포 및 거의 전 세포질이 endoplasmic reticulum으로 채워진 세포들이 관찰되었다. 3. 위의 사실들로 미루어 양서류 피부점액선의 선상피는 mitochondria가 많은 미분화세포와 점액분비과립을 많이 함유한 성숙세포 및 전 세포질이 거의 rough endoplasmic reticulum으로 채워진 분비후기세포로 구성된다고 생각된다.

• Applied Microscopy
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• 제24권1호
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• pp.28-40
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• 1994

Xerostomia and xerophthalmia are delicate or serous side effects, occuring when the radiotherapy is administered to the head and neck cancer patient. It is known that the cause of the above side effect is radiosensitivity of serous cells. In this study, the ultrastructural features of the parotid glands of the X-irradiated rats were observed. Sprague-Dawley rats weighing 200-250g each were anesthetized with sodium thiopental, and placed on the Mitsubishi linear accelerator. Only the head and neck areas of animals were exposured at the distance of 80cm, within the area of $30X30cm$, in the depth of 1cm, with the speed of 200R/min. Total doses applied were 3,000R or 6,000R depending on the experimental groups. Animals were sacrificed on the 6th hour, 2nd day and 6th day after the irradiation. Parotid glands were fixed in the 2.5% glutaraldehyde-1.5% paraformaldehyde solution, and followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture, and ultrathin sections were cut. Sections were contrasted with the solution of uranyl acetate and lead citrate, and observed with JEM 100 CX-II electron microscope. The results were as follows: 1. Normal parotid acinar cells are two types; the light and the dark acinar cells. The light acinar cell contains dense secretory granules, whereas dark acinar cells contains granules of medium density with some darker spots within them, or other cells contain granules of medium density with darker rims. 2. Six hours after the irradiation, many acinar cells were degenerated showing variable stages of cytolytic bodies, light bodies, or dense degenerations. Within the acinar cell, Golgi apparatus and granular endoplasmic reticula were most severely altered elements. Granules showed more contrasting densities and irregularities. 3. Two days after the irradiation, some cytolytic bodies, and focal lucent degeneration of cytoplasm, and fine granular alteration of cytoplasmic matrix were pronounced. But other elements including secretory granules are rather looked unlatered. 4. Six days after the irradiation, most severe alterations were seen. Many intracellular canaliculi (or secretion figures), quanta of cytoplasm containing secretion antecedants, severely irregular luminal border, and again contrasting density of secretory granules showing tigroid spots or dense rims were noted. And myoepithelial degenerations were observed not uncommonly. 5. Irregular densities of secretory granules were interpreted as abnormal components of protein or carbohydrate portion are synthesized or abnormally metabolized under severe X-irradiation. 6. Myoepithelial degeneration and related alteration of nerve endings, etc., were suggested as the other causes of xerostomia following X-irradiation. 7. It is requested that radiation doses should be arranged, considering in mind not only the sensitivity of acinar cells but also the myoepithelial and neural functions.

• 한국수산과학회지
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• 제13권4호
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• pp.135-144
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• 1980

우리나라 낙동강 하류에 서식하는 뻘조개, Anodonta (Sinanodonta) woodiana의 생식소발달 과정 및 생식주기 그리고 임란기 등을 조사하였다. 1. 생식소는 내장낭의 간중사선 하방으로부터 족부의 외벽근층내에 있는 섬유성 망상결제조직까지 분포되어 있다. 난소는 많은 난소소낭으로 구성되어 있는데, 이들 소낭의 내공상피가 생식상피 기능을 하고 있다. 2. 초기난소 몇 정소의 생식상피상에는 호산성 과립세포와 부분화 간충조직들이 풍부하게 나타나고 있으며, 이들은 생식소 발달과 함께 그 량이 감소하고 있다. 3. 산란은 고수온기인 8월과 9월을 제외하고 년중 일어나고 있는데 1월에서 3월과, 6월에서 7월의 2회에 걸쳐 성기를 나타내고 있다. 4. 보육낭내에 trochophore 유생을 임란한 개체는 8월과 9월을 제외하고 년중 나타났으며 glochidia 유생을 임란한 개체는 9월을 제외하고 연중 나타났는데, 5월이 $72.7\%$ 년중 가장 높은 임란율을 나타내고 있다.

• 치과방사선
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• 제20권2호
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• pp.171-182
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• 1990

The purpose of this study was to investigate the effects of irradiation on the striated duct cells of the rat submandibular gland ductal tissues which control the characteristics of saliva. For this study, the experimental group was composed of 36 irradiated Sprague Dawley strain rats divided into 8 subgroups 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours after irradiation. 4 non-irradiated rats were used as the control group. The experimental animals were singly irradiated with a dose of 18Gy gamma ray to their head and neck region by the Co-6- teletherapy unit and sacrificed after each experimental duration. The specimens were examined with a light microscope with an H-E stain and with a trans- mission electron microscope. The results of this study were as follows. In the light micrograph, a severe atrophic change occurred in the striated duct cells at 2hours after irradiation and gradual recovery occurred from 6 hours after irradiation. 2. The nuclear chromosomes of the striated duct cells were changed granular at 2 hours after irradiation. Recovery was observed at 6 hours after irradiation. Nuclear bodies were also observed from 3 hours after irradiation. 3. The mitochondria of the striated duct cells had indistinct cristae at 2 hours after irradiation, and were degenerated or swollen at 3 hours after irradiation. They recovered, however, from 6 hours, with an increasing number at 48 hours and a regular arrangement was observed at 72 hours after irradiation. 4. The microvilli showed atrophic changes at 2 hours after irradiation and were almost lost at 3 hours after irradiation. They were observed again from 48 hours after irradiation. 5. The rough endoplasmic reticulum and golgi body were not apparent at 1 hour after irradiation and were dilated with degeneration 2 hours after, but intact rough endoplasmic reticulum were observed from 3 hours after irradiation and developed well at 24 hours after irradiation. By the result of this study, showing a mild change in the functional morphology of the salivary striated duct cells immediately following irradiation, it is considered that the many complications which occur after radiation therapy, will disappear in time with the histological and the functional recovery of the glandular tissues.

• Applied Microscopy
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• 제30권3호
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• pp.303-310
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• 2000

문치가자미의 피부 상피층은 지지세포, 선세포 그리고 부속세포들로 구성된다. 지지세포는 표면세포, 중간세포 및 기저세포로 구분된다. 지지세포들의 세포질은 공통적으로 피질부와 수질부로 나누어지는때, 수질부에는 세포 소기관의 발달이 현저하며, 피질부에는 미세섬유의 발달이 뚜렷하다. 선세포들은 상피의 표면층과 중간층에 존재한다. 점액세포의 세포질은 AB-PAS에 청색으로 반응하였다. 곤봉방세포는 세포질에 원형의 중심공포와 발달된 미세섬유들을 가진다. 과립세포는 주로 중간층과 기저층에 존재하고, 세포질의 대부분은 막을 가진 전자밀도가 높은 과립들이 차지한다. 염세포는 주로 표층에 위치하며, 세포질의 대부분은 잘 발달된 미토콘드리아들이 차지한다. 색소세포는 세포질에 존재하는 함유물의 전자밀도에 따라 세 종류로 구분할 수 있다.