• 한국환경과학회지
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• 제7권5호
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• pp.697-705
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• 1998

$Pb^{2+}$ removal capacity and initial $Pb^{2+}$ removal rate were compared between non-biomaterials (granular activated carbon, powdered activated carbon, ion exchange resin, zeolite) and biomaterials (activated sludge, Aureobasidium pullulans, Saccharomyces cerevisiae). The $Pb^{2+}$ removal capacity of biomaterials were greater than that of non-biomaterials, generally. The $Pb^{2+}$ removal capacities of non-biomaterials and biomaterials were shown on the order of ion exchange resin ＞ zeolite ＞ granular activated carbon ＞ powdered activated carbon and A. pullulans ＞ S. cerevisiae ＞ activated sludge, respectively. In the initial $Pb^{2+}$ removal rate, the non-biomaterials showed powdered activated carbon ＞ granular activated carbon ＞ zeolite ＞ ion exchange resin and the biomaterials showed A. pullulans ＞ activated sludge ＞ S. cerevisiae. Comparing the $Pb^{2+}$ removal capacity and initial $Pb^{2+}$ removal rate of activated sludge with those of other non-biomaterials and biomaterials, activated sludge may have an availability on the removal of heavy metal ions by the economical and pratical aspects.

• 한국물환경학회지
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• 제29권4호
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• pp.497-503
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• 2013

The morphological characteristics of granules, which were generated in lab-scale sequencing batch reactor (SBR) for simultaneous nitrogen and phosphorus removal with denitrifying phosphorus accumulating organism (dPAO) were identified. Granular sludge was fully developed in the anaerobic-anoxic (An-Ax) SBR after 180 days of SBR operation. The average diameter of granular sludge was 2.2 mm and rod-type organisms dominated in the granules. In addition, about 1.0 mm of white precipitate was observed in the core of the granule, and the material was confirmed that it is very similar to hydroxyapatite $(HAP;\;Ca_5(PO_4)_3(OH))$ by X-ray diffraction) analysis.

• Environmental Engineering Research
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• 제19권4호
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• pp.355-361
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• 2014

The wastewater treatment with a two-phase expanded granular sludge bed (EGSB) system for anaerobic degradation of acetate, benzoate, terephtalate and p-toluate from purified terephtalic acid (PTA) production was studied. The feasibility and effectiveness of the system was evaluated in terms of organic oxidation by chemical oxygen demand (COD), gas production, bacterial adaptability and stability in the granular sludge. Average removal efficiencies 93.5% and 72.7% were achieved in the EGSB reactors under volumetric loading rates of $1.0-15kg-COD/m^3/day$ and terephtalate and p-toluate of 351-526 mg/L, respectively. Gas production reached total methane production rate of 0.30 L/g-COD under these conditions in the sequential EGSB reactor system. Higher strength influent COD concentration above 4.8 g-COD/L related to field conditions was fed to observe the disturbance of the EGSB reactors.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권1호
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• pp.54-57
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• 2003

H$_2$-producing bacteria were isolated from anaerobic granular sludge. Out of 72 colonies (36 grown under aerobic conditions and 36 under anaerobic conditions) arbitrarily chosen from the agar plate cultures of a Suspended sludge, 34 colonies (15 under aerobic conditions and 19 under anaerobic conditions) produced H2 under anaerobic conditions. Based on various biochemical tests and microscopic observations, they were classified into 13 groups and tentatively identified as follows: From aerobic isolates, Aeromonar spp. (7 strains), Pseudomonas spp. (3 strains), and Vibrio spp. (5 strains); from anaerobic isolates, Actinomyces spp. (11 Strains), Clostridium 5pp. (7 strains). and Porphyromonas sp. When glucose was used as the carbon substrate, all isolates showed a similar cell density and a H$_2$ production yield in the batch cultivations after 12 h (2.24-2.74 OD at 600 nm and 1.02-1.22 mol H$_2$/mol glucose, respectively). The major fermentation by-products were ethanol and acetate for the aerobic isolates, and ethanol, acetate and propionate for the anaerobic isolates. This study demonstrated that several H$_2$ producers in an anaerobic granular sludge exist En large proportions and their performance in terms of H$_2$ production is quite similar.

• Membrane and Water Treatment
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• 제15권1호
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• pp.1-9
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• 2024

Biological nutrient removal is gaining increasing attention in wastewater treatment plants; however, it is adversely affected by low temperatures. This study examined temperature effects on nutrient removal and morphological stability of the granular and denitrifying phosphorus accumulating organisms (PAO and DPAO, respectively) using sequencing batch reactors (SBRs) at 5, 10, and 20 ℃. Lab-scale SBRs were continuously operated using anaerobic-anoxic and anaerobic-oxic cycles to develop the PAO and DPAO granules for 230 d. Sludge granulation in the two SBRs was observed after approximately 200 d. The average removal efficiency of soluble chemical oxygen demand (SCOD) and PO₄^3--P remained >90% throughout, even when the temperature dropped to 5 ℃. The average removal efficiency of NO₃^--N remained >80% consistently in DPAO SBR. However, nitrification drastically decreased at 10 ℃. Hence, the removal efficiency of NH₄⁺-N was decreased from 99.1% to 54.5% in PAO SBR. Owing to the increased oxygen penetration depth at low temperatures, the influence on nitrification rates was limited. The granule in DPAO and PAO SBR was observed to be unstable and disintegrated at 10 ℃. In conclusion, morphological characteristics showed that changed conversion rates at low temperatures in aerobic granular sludge altered both nutrient removal efficiencies and granule formation.

• 한국환경과학회지
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• 제30권12호
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• pp.1081-1092
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• 2021

In this study, the effect on the stability of Aerobic Granular Sludge (AGS) caused by an AGS separator was investigated. The AGS separator was a hydrocyclone. The main factors of the AGS separator were filter pore size (0.125~0.600 mm), conical-to-cylindrical ratio (1.5~3.0), and operating time (1~20 min). The AGS/mixed liquor suspended solid (MLSS) ratio gradually increased to 0.500 mm (AGS/MLSS: 84.3±3.0%). AGS was best separated at the conical-to-cylindrical ratio of 2.5 (AGS/MLSS: 84.7±3.3%). As the operating time increased, the AGS separation performance also tended to increase. The shortest AGS separator run time, but the highest AGS separation performance was 10 min (87.0±2.5%). AGS stability was evaluated by operating the selected AGS separator and sequencing batch reactor. The average removal efficiencies of TOC, TCOD_Cr, SS, TN, and TP were 95.7%, 96.9%, 93.0%, 89.0%, and 96.2%, respectively, which met the effluent standards in Korea. In addition, the AGS/MLSS ratio tended to remain constant, and the sludge volume index demonstrated a tendency to decrease from 140 mL/g to 70 mL/g. During the operation, the particles of AGS in optical microscope observations gradually increased.

• 유기물자원화
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• 제15권2호
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• pp.136-146
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• 2007

상향류 혐기성 블랭킷 반응조를 이용한 매립지 침출수 처리시 입상슬러지 첨가 유무에 상관없이 성공적인 처리가 가능하였다. 입상슬러지의 첨가는 초기 운전기간를 현저히 단축할 수 있었다. 수리학적 체류시간 1일과 $4-8kgCOD/m^3.d$ 의 유기물 부하율에서 Control 반응조와 Granule 반응조의 COD 제거율은 90% 이상을 유지하였다. 실험기간 동안 입상슬러지 첨가 유무에 상관없이 슬러지 표면과 반응조 벽면에 무기 침전물이 축적되었다. 비메탄 활성도는 미생물의 기질에 대한 적응도와 유기물 부하량이 증가함에 따라 증가하였다. Granule 반응조의 최대 비메탄 활성도의 값은 $0.57gCOD/g{\cdot}VSS{\cdot}.d$로 나타났다. 비록 본 연구에서는 과도한 무기물 축적으로 인한 비메탄 활성도의 저감은 발생하지 않았으나 무기물의 제거를 위하여 유입수의 전처리 공정이 필요할 것으로 판단된다.

• 한국환경과학회지
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• 제5권3호
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• pp.387-398
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• 1996

Granular sludge formation and it's activity change are the most important factors in achieving successful start-up and operation of UASB reactor. Nevertheless, the detailed mechanism is still unknown. On the basic of the experiments in laboratory-scale UASB reactor, the effect of hydrogen partial pressure on sludge granulation was evaluated. Size distribution method and specific metabolic activity of the sludge with the operation time were used as a means for estimating the degree of the sludge granulation. At the constant hydrogen loading, the granulation increased as starvation periods in hydrogen supply increased, resulting in high organic removal efficiency. It was evidient that hydrogen play very important role in granulation and sludge granulation was achieved through mutual symbiosis between hydrogen utilizing bacteria and hydrogen producing bacteria under the hydrogen dificient conditions. Key words : granular sludge, UASB reactor, hydrogen partial pressure.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.199-204
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• 2006

The purpose of this work was to evaluate the development of the anammox process by the use of granular sludge selected from a digestion reactor as a potential seed source in a lab-scale UASB (upflow anaerobic sludge blanket) reactor system. The reactor was operated for approximately 11 months and was fed by synthetic wastewater. After 200 days of feeding with $NH_4^+\;and\;NO_2^-$ as the main substrates, the biomass showed steady signs of ammonium consumption, resulting in over 60% of ammonium nitrogen removal. This report aims to present the results and to more closely examine what occurs after the onset of anammox activity, while the previous work described the start-up experiment and the presence of anammox bacteria in the enriched community using the fluorescence in situ hybridization (FISH) technique. By the last month of operation, the consumed $NO_2^--N/NH_4^+-N$ ratio in the UASB reactor was close to 1.32, the stoichiometric ratio of the anammox reaction. The obtained results from the influent-shutdown test suggested that nitrite concentration would be one key parameter that promotes the anammox reaction during the start-up enrichment of anammox bacteria from granular sludge. During the study period, the sludge color gradually changed from black to red-brownish.

• 환경위생공학
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• 제13권2호
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• pp.95-105
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• 1998

This research was investigated which denitrification of wastewater containing nitrate, using upflow anaerobic sludge blanket process. The upflow anaerobic sludge blanket process is also used for both artifical and industrial wastewater. Main ingredients investigated in the artifical and industrial wastewater experiment were the determination of optimum organism/nitrate ratios, nitrate removal efficiency by various hydrogen donor addition and characteristics of granular sludge and gas production in case of various hydrogen donor addition. From the experimental results the following conclusions were made: In case of adding methanol, ethanol and sodium acetate as hydrogen donor granular sludge was formed 50 days after seeding. Average diameter of granular sludge was 4.0 mm and settling velocity was 37 cm/min. Production rate of gas 3.3 L/d in case of adding methanol as hydrogen donor in wastewater containing 150mg/L nitrate. However adding ethanol and sodium acetate as hydrogen donor, gas production rate were 2.2-2.7L/d respectively. In case of adding methanol as hydrogen donor treatability of artifical wastewater contained 150mg/L as nitrate was about 93%. But in addition of sodium acetate in wastewater contained 40mg.L as nitrate, nitrate removal efficiency was 80%.