• 제목/요약/키워드: Graduate school life

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MORPHOLOGICAL, LECTIN-HISTOCHEMICAL AND PCNA-IMMUNO HISTOCHEMICAL COMPARISONS OF THE TESTICULAR TISSUES BETWEEN THE FIRST WAVE AND THE REINITIATION OF SPERMATOGENESIS AFTER EXPOSURE TO A SHORT PHOTOPERIOD

  • NAKAMURA Ryusuke;MIZUKAMI Takuo;FUJISAWA Masahiko;KANAI Yoshiakira;KUROHMARU Masamichi;HAYASHI Yoshihiro
    • 한국식품위생안전성학회:학술대회논문집
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    • 한국식품위생안전성학회 2001년도 The Asia-Pacific Conference on Reproductive Biology and Environmental Sciences
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    • pp.232-234
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    • 2001

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Lipoteichoic Acid Isolated from Weissella cibaria Increases Cytokine Production in Human Monocyte-Like THP-1 Cells and Mouse Splenocytes

  • Hong, Yi-Fan;Lee, Yoon-Doo;Park, Jae-Yeon;Kim, Seongjae;Lee, Youn-Woo;Jeon, Boram;Jagdish, Deepa;Kim, Hangeun;Chung, Dae Kyun
    • Journal of Microbiology and Biotechnology
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    • 제26권7호
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    • pp.1198-1205
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    • 2016

  • Lactic acid bacteria (LAB) have beneficial effects on intestinal health and skin diseases. Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is known to induce the production of several cytokines such as TNF-α, IL-1β, and IL-8 and affect the intestinal microflora, anti-aging, sepsis, and cholesterol level. In this study, Weissella cibaria was isolated from Indian dairy products, and we examined its immune-enhancing effects. Live and heat-killed W. cibaria did not induce the secretion of immune-related cytokines, whereas LTA isolated from W. cibaria (cLTA) significantly increased the secretion of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. cLTA increased the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells, p38 mitogen-activated protein kinases, and c-Jun N-terminal kinases in THP-1 cells. The secretion of TNF-α and IL-6 was also increased in the cLTA-treated mouse splenocytes. These results suggest that cLTA, but not W. cibaria whole cells, has immune-boosting potential and can be used to treat immunosuppression diseases.

  • https://doi.org/10.4014/jmb.1601.01047 인용 PDF KSCI KPUBS HTML

Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605

  • Ichinose, Yuki;Nishimura, Takafumi;Harada, Minori;Kashiwagi, Ryota;Yamamoto, Mikihiro;Noutoshi, Yoshiteru;Toyoda, Kazuhiro;Taguchi, Fumiko;Takemoto, Daigo;Matsui, Hidenori
    • The Plant Pathology Journal
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    • 제36권2호
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    • pp.148-156
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    • 2020

  • Pseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ΔmexB, ΔmexF, and ΔmexBΔmexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitivity to these compounds tended to increase in ΔmexB and ΔmexBΔmexF mutants. The ΔmexBΔmexF mutant was also sensitive to 2 M acetovanillone. The mexAB-oprM was constitutively expressed, and activated in the ΔmexF and ΔmexBΔmexF mutant strains. The swarming and swimming motilities were impaired in ΔmexF and ΔmexBΔmexF mutants. The flood inoculation test indicated that bacterial populations in all mutant strains were significantly lower than that of WT, although all mutants and WT caused similar disease symptoms. These results indicate that MexAB-OprM extrudes plant-derived catechol, acetovanillone, or coumarin, and contributes to bacterial virulence. Furthermore, MexAB-OprM and MexEF-OprN complemented each other's functions to some extent.

  • https://doi.org/10.5423/PPJ.OA.11.2019.0273 인용 PDF KSCI