• Title/Summary/Keyword: Gradients

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Quantitative Analysis of Single Bacterial Chemotaxis Using a Hydrodynamic Focusing Channel (유체역학적 집속 효과를 이용한 단일 박테리아 주화성의 정량적 분석)

  • Jeon, Ho-Jeong;Lee, Yong-Ku;Jin, Song-Wan;Koo, Sang-Mo;Lee, Chang-Soo;Yoo, Jung-Yul
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.31 no.3 s.258
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    • pp.209-216
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    • 2007
  • Bacterial chemotaxis is essential to the study of structure and function of bacteria. Although many studies have accumulated the knowledge about chemotaxis in the past, the motion of a single bacterium has not been studied much yet. In this study, we have developed a device microfabricated by soft lithography and consisting of microfluidic channels. The microfluidic assay generates a concentration gradient of chemoattractant linearly in the main channel by only diffusion of the chemicals. Bacteria are injected into the main channel in a single row by hydrodynamic focusing technique. We measured the velocity of bacteria in response to a given concentration gradient of chemoattractant using the microfludic assay, optical systems with CCD camera and simple PTV (Particle Tracking Velocimetry) algorithm. The advantage of this assay and experiment is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by statistically analyzing the velocity at the same time. Specifically, we measured and analyzed the motility of Escherichia coli strain RP437 in response to various concentration gradients of L-aspartate statistically and quantitatively by using this microfluidic assay. We obtained the probability density of the velocity while RP437 cells are swimming and tumbling in the presence of the linear concentration gradient of L-aspartate, and quantified the degree of chemotaxis by analyzing the probability density.

People Tracking and Accompanying Algorithm for Mobile Robot Using Kinect Sensor and Extended Kalman Filter (키넥트센서와 확장칼만필터를 이용한 이동로봇의 사람추적 및 사람과의 동반주행)

  • Park, Kyoung Jae;Won, Mooncheol
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.4
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    • pp.345-354
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    • 2014
  • In this paper, we propose a real-time algorithm for estimating the relative position and velocity of a person with respect to a robot using a Kinect sensor and an extended Kalman filter (EKF). Additionally, we propose an algorithm for controlling the robot in the proximity of a person in a variety of modes. The algorithm detects the head and shoulder regions of the person using a histogram of oriented gradients (HOG) and a support vector machine (SVM). The EKF algorithm estimates the relative positions and velocities of the person with respect to the robot using data acquired by a Kinect sensor. We tested the various modes of proximity movement for a human in indoor situations. The accuracy of the algorithm was verified using a motion capture system.

Development of Signal Processing Technique of Digital Speckle Tomography for Analysis of Three-Dimensional Density Distributions of Unsteady and Asymmetric Gas Flow (비정상 비대칭 기체 유동의 3차원 밀도 분포 분석을 위한 디지털 스펙클 토모그래피 기법의 신호 처리 기술 개발)

  • Baek, Seung-Hwan;Kim, Yong-Jae;Ko, Han-Seo
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.2
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    • pp.108-114
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    • 2006
  • Transient and asymmetric density distributions of butane flow have been investigated from laser image signals by developed three-dimensional digital speckle tomography. Moved signals of speckles have been captured by multiple CCD images in three angles of view simultaneously because the flows were asymmetric and transient. The signals of speckle movements between no flow and downward butane flow from a circular half opening have been calculated by a cross-correlation tracking method so that those distances can be transferred to deflection angles of laser rays fur density gradients. The three-dimensional density fields have been reconstructed from the fringe shift signal which is integrated from the deflection angle by a real-time multiplicative algebraic reconstruction technique (MART).

Complete Repair of Tetralogy of Fallot in Neonate or Infancy (신생아및 영아기 활로씨 사징증의 완전 교정술)

  • 이정렬
    • Journal of Chest Surgery
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    • v.25 no.1
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    • pp.32-41
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    • 1992
  • From August 1982 to December 1991, 58 consecutive infants with tetralogy of Fallot underwent primary repair. Age ranged from 22 days to twelve months [n=58, 8.7$\pm$2.7 months] and body weight from 3.1 to 13 kilograms [n=58, 7.8$\pm$1.7 kilograms]. Qne infant had absence of the pulmonary valve; one had Ebstein`s anomaly and one had supramitral ring. Thirty-two patients [56%] experienced anoxic spell. Preoperative pulmonary artery indices were measured in 38 cases, ranging 126-552mm2/M2BSA[n=38, 251$\pm$79mm2/M2BSA]. All infants required a right ventricular outflow tract patch; in 41, the patch extended across the pulmonary valve annulus, in 13 of them, monocusps were constructed. All had patch closure of ventricular septal defect. Two infants had REV operation for avoiding injury to the canal branch of the right coronary artery which cross the right ventricular out flow tract. Post repair PRV/LV were measured at operating room in 40 cases, which revealed mean value of 0.49$\pm$0.12 [range: 0.25-0.74]. The hospital mortality was 10.3% [6 patients], and causes of deaths were right heart failure due to sustained right ventricular hypertension[4] and right ventricular outflow tract obstruction, intractablesuraventricular tachyarrhythmia[1], hypoxia[1] due to residual right to left shunt across the atrial septal defect in patient associated with Ebstein`s anomaly. All infants were doing well at follow-up from 1 to 101 months[20.6 months /patient, 1, 072 patient-month] Serial postoperative echocardiograms revealed no residual ventricular septal defects and estimated RVOT gradients between 0 and 40 mmHg except 3 cases [50, 50, 60 mmHg]. There were no late deaths and late ventricular arrhythmias or congestive heart failure. Redo operations were done in 2 cases because of residual right ventricular outflow tract obstruction. This experience with infants with tetralogy of Fallot suggests that, if mortality is tolerable, eletive repair of tetralogy of Fallot could be reasonably undertaken during the first year of life, and even better results could be anticipated along with improvement of methods of myocardial protection and postoperative care.

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More about Taxonomic Sufficiency: A Case Study using Polychaete Communities in a Subtropical Bay Moderately Affected by Urban Sewage

  • Muniz Pablo;Pires-Vanin Ana M. S.
    • Ocean Science Journal
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    • v.40 no.3
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    • pp.127-143
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    • 2005
  • The taxonomic sufficiency approach has been proposed as a surrogate for the typical analysis of species-abundance data, especially in conditions involving prominent pollution gradients. Here, we evaluate the use of taxonomic sufficiency with infralittoral macrobenthic data derived from samples taken in a moderate polluted subtropical environment in southeastern Brazil, analysing five taxonomic levels and including two functional levels throughout polychaete feeding guilds and trophic groups. The data were collected seasonally at nine stations and studied for two abundance data series (0.5 and 1.0 mm sieve mesh-size). The results showed a similar ordination pattern between the two sieve mesh-size, but with the 0.5 mm sieve data a different pattern was observed during austral summer. A slight loss of information was detected using genus, family, polychaete species and their feeding guilds as taxonomic/functional units. These results together with those of the cost! benefit ratio, suggested that the family level seemed to be sufficient to detect the impact caused by moderate pollution in this shallow-water, subtropical environment. In additional, through the use of feeding guilds, similar patterns are obtained. Correlation analysis showed that chlorophyll a, total organic matter, zinc, and chromium sediment content were the variables that best explained the biological pattern observed and not always the best correlation coefficient occurring at the species level. The feeding guild approach seems to be useful and generates interpretable results similar to those obtained with the species level of the whole macroinfauna. The results showed an important cost reduction in the sample processing, suggesting that it is possible to adopt a coarser taxonomic level monitoring program even in species-rich communities.

Aortic Valve Replacement Using Continuous Suture Technique in Patients with Aortic Valve Disease

  • Choi, Jong Bum;Kim, Jong Hun;Park, Hyun Kyu;Kim, Kyung Hwa;Kim, Min Ho;Kuh, Ja Hong;Jo, Jung Ku
    • Journal of Chest Surgery
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    • v.46 no.4
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    • pp.249-255
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    • 2013
  • Background: The continuous suture (CS) technique has several advantages as a method for simple, fast, and secure aortic valve replacement (AVR). We used a simple CS technique without the use of a pledget for AVR and evaluated the surgical outcomes. Materials and Methods: Between October 2007 and 2012, 123 patients with aortic valve disease underwent AVR alone (n=28) or with other concomitant cardiac procedures (n=95), such as mitral, tricuspid, or aortic surgery. The patients were divided into two groups: the interrupted suture (IS) group (n=47), in which the conventional IS technique was used, and the CS group (n=76), in which the simple CS technique was used. Results: There were two hospital deaths (1.6%), which were not related to the suture technique. There were no significant differences in cardiopulmonary bypass time or aortic cross-clamp time between the two groups for AVR alone or AVR with concomitant cardiac procedures. In the IS group, two patients had prosthetic endocarditis and one patient experienced significant perivalvular leak. These patients underwent reoperations. In the CS group, there were no complications related to the surgery. Postoperatively, the two groups had similar aortic valve gradients. Conclusion: The simple CS method is useful and secure for AVR in patients with aortic valve disease, and it may minimize surgical complications, as neither pledgets nor braided sutures are used.

Reduction of the the Ground Surface Potential Gradients by Installing Auxiliary Grounding Grids (보조접지그리드의 시설에 의한 대지표면전위경도의 저감)

  • 이승칠;엄주홍;이복희;김효진
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.2
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    • pp.121-129
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    • 2002
  • The present paper describes a technique for installing an effective grounding grids, the major objective is forced on the experimental evaluation of the performance and characteristics with the arrangement and installation method for grounding grids consisting of the means to protect electric shock, electronics and computerized facilities against lightning, switching and ground fault surges. The study is oriented on two major areas: (1) the analysis of the ground surface potential gradient with the arrangement of grounding grids, (2) the control of the dangerous ground surface potential rise. The experiments wee carried out with the impulse currents as a function of the installation method or arrangement of grounding grids. An installation method of the inclined auxiliary grounding grid was proposed to overcome the drawbacks of equally spared grounding grids, i.e. an appropriate design concept far the installation of grounding grids was found out, It has been shown that the installation of the intwined auxiliary grounding grid can also result in a mere than 50% decrease in the maximum potential gradient on the ground surface and enhance the level of safety for persons and electronic equipments..

Real Time Traffic Light Detection Algorithm Based on Color Map and Multilayer HOG-SVM (색상지도와 멀티 레이어 HOG-SVM 기반의 실시간 신호등 검출 알고리즘)

  • Kim, Sanggi;Han, Dong Seog
    • Journal of Broadcast Engineering
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    • v.22 no.1
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    • pp.62-69
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    • 2017
  • Accurate detection of traffic lights is very important for the advanced driver assistance system (ADAS). There have been many research developments in this area. However, conventional of image processing methods are usually sensitive to varying illumination conditions. This paper proposes a traffic light detection algorithm to overcome this situation. The proposed algorithm first detects the candidates of traffic light using the proposed color map and hue-saturation-value (HSV) Traffic lights are then detected using the conventional histogram of oriented gradients (HOG) descriptor and support vector machine (SVM). Finally, the proposed Multilayer HOG descriptor is used to determine the direction information indicated by traffic lights. The proposed algorithm shows a high detection rate in real-time.

Spatial and Temporal Variability of Water Quality in Korean Dam Reservoirs

  • Lim, Go-Woon;Lee, Sang-Jae;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.42 no.4
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    • pp.452-464
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    • 2009
  • The objectives of this study were to evaluate spatial and temporal variability of water quality in 10 reservoirs and identify the key nutrients (N, P) influencing chlorophyll-a (CHL) along with analysis of empirical models and zonal patterns of total phosphorus (TP) and CHL. We analyzed total nitrogen (TN), TP, CHL, water clarity (Secchi depth, SD), and evaluated potential limiting nutrient using ambient N:P ratios and previous criteria of ambient nutrients. Water clarity and CHL varied largely depending on the seasonal monsoon and type of reservoir, but trophic state was diagnosed as eutrophy, base on mean CHL in most reservoirs. The peak of TP did not match the contents of CHL due to rapid flushing during the high run-off period. In the reservoir of DR, regression coefficient in the $P_r$ was 0.510 but was 0.159 in the $M_o$, while the TP-CHL relation in the YR increased during the monsoon compared to the premonsoon. The regression coefficient in the $P_r$ was not statistically significant but the value of $M_o$ was 0.250. TP showed similar longitudinal zonal gradients among the reservoirs of DR, YR and JR. Empirical models of TP-CHL, based on overall data, showed that CHL was determined by phosphorus($R^2=0.244$, p=0.0019). Regression analysis of CHL-SD showed a stronger linear fit ($R^2=0.638$, p<0.001) than the TP-CHL model.

Water Quality in Artificial Reservoirs and Its Relations to Dominant Reservoir Fishes

  • Hwang, Yoon;Han, Jeong-Ho;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.42 no.4
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    • pp.441-451
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    • 2009
  • The major objectives of this study were to evaluate trophic state of reservoirs using major water quality variables and its relations in terms of trophic guilds and tolerance guilds with dominant lentic fishes. For this study, we selected 6 artificial reservoirs such as Namyang Reservoir ($N_yR$), Youngsan Reservoir ($Y_sR$), Daechung Reservoir ($D_cR$), Chungju Reservoir ($Cj_R$), Chungpyung Reservoir ($C_pR$), and Paldang Reservoir ($P_dR$), and collected fish during 2000~2007 along with data analysis of water quality monitored by the ministry of environment, Korea. Biological oxygen demand (BOD) and chemical oxygen demand (COD), indicators of organic matter pollution, varied depending on types of the reservoirs and the spatial patterns in terms of trophic gradients were similar to patterns of nutrients, Secchi depth and chlorophyll-a. Analysis of trophic state index (TSI) showed that reservoirs of $D_cR$ and $C_jR$ were mesotrophy and other 4 reservoirs were eutrophic state. The relations of trophic relations showedthat TSI (Chl-a) had a positive linear function [TSI (CHL)=0.407 TSI (TP)+28.2, n=138, p<0.05] with TSI (TP) but had a weak relation with TSI (TN). Also, TSI (TP) were negatively correlated ($R^2=0.703$, p<0.05) with TSI (SD), whereas TSI (TN) was not significant (p>0.05) relations with TSI (SD). Tolerance guilds of lentic fishes, based on three types of the reservoirs, reflected the exactly water quality in the TN, TP, BOD, and COD, and similar trends were shown in the fish feeding/trophic guilds.