• Journal of Plant Biology
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• v.30 no.3
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• pp.205-213
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• 1987

Soybean, inoculated with effective Rhizobium japonicum 110, were grown by sand culture with nutrient solution containing either of 0, 1, 3, 10 or 30mM NO3-/l, and analyzed growth characteristics, NR activity, N2-fixation activity, and changes of ureide contents during the growing period. The amount of nodule formation decreased abruptly by nitrate treatment, the maximum nodule dry weight was 1.59, 1.05, 0.78, 0.09 and 0.008 g plant-1, respectively for each treatment on the 98th day. Specfic activity of N2-fixation showed the maximum rates of 140, 101, 37, 5 and 2.2 nM dw.mg-1.hr-1, respectively for each treatment in the earlier growth period. The maximum acetylene reduction activity on the 98th day after sowing was 81.5, 35.3, 14.3, 0.1 and 0.0045 $\mu$M C2H4 plant-1.hr-1, respectively for 0, 1, 3, 10 and 30 mM of NO3- gradients. Nitrate reduction activity increased along with nitrate gradients, and decreased abruptly with age. Relative abundance of ureides in plant organs was high in reproductive growth, and showed the maximum value in fully symbiotic dependent plant. Relative abundance of ureides in stem is a useful indication for the evaluation of nitrogen fixation in nodules of symbiotic plant.

• BioChip Journal
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• v.12 no.4
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• pp.294-303
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• 2018

Shear stress occurs in flowing liquids, especially at the interface of a flowing liquid and a stationary solid phase. Thus, it occurs inside the artery system of the human body, where it is responsible for a number of biological functions. The shear stress level generally remains less than $70dyne/cm^2$ in the whole circulatory system, but in the stenotic arteries, which are constricted by 95%, a shear stress greater than $1,000dyne/cm^2$ can be reached. Methods of researching the effects of shear stress on cells are of large interest to understand these processes. Here, we show the development of a microfluidic device for generating shear stress gradients. The performance of the shear stress gradient generator was theoretically simulated prior to experiments. Through simple manipulations of the liquid flow, the shape and magnitude of the shear stress gradients can be manipulated. Our microfluidic device consisted of five portions divided by arrays of micropillars. The generated shear stress gradient has five distinct levels at 8.38, 6.55, 4.42, 2.97, and $2.24dyne/cm^2$. Thereafter, an application of the microfluidic device was demonstrated testing the effect of shear stress on human umbilical vein endothelial cells.

• Journal of information and communication convergence engineering
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• v.17 no.1
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• pp.67-73
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• 2019

In the current era of digital technology, and with the help of existing software, digital photo manipulation is becoming easier and faster. One example of this is the development of powerful image processing software that makes it easy for a digital image to be manipulated and edited. It is therefore very important to protect and maintain public trust in digital images. Several methods have been developed to detect image manipulation. In this paper, we compare two methods for detecting image duplication due to copy-move actions, namely the polar coordinate system and the histogram of oriented gradients methods. The former is a method based on the transfer of a Cartesian image to a polar form, making it easy to tell whether there are objects that have undergone a copy/move in an image, while the latter is a method for retrieving information related to the distribution, which uses a target in the local area as a tool to represent the shape of the target. We compare the accuracy, speed and memory usage of these two methods.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.2-4
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• 2021

In a distributed machine learning system, sharing gradients was considered safe because it did not share original training data. However, recent studies found that malicious attacker could completely restore the original training data from shared gradients. Gradient Leakage Attack is a technique that restoring original training data by exploiting theses vulnerability. In this study, we present the image transformation method based on Discrete Cosine Transform to defend against the Gradient Leakage Attack on the federated learning setting, which training in local devices and sharing gradients to the server. Experiment shows that our image transformation method cannot be completely restored the original data from Gradient Leakage Attack.

• Journal of Soil and Groundwater Environment
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• v.13 no.2
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• pp.12-20
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• 2008

This study was conducted to investigate the spatial distributions of chromated copper arsenate (CCA) metals in soils around three log structures and a sound barrier, all built with CCA-treated wood. Sixty-six surface and 35 profile soil samples were collected around the wood structures in addition to 13 background soil samples. The concentrations of chromium, copper, and arsenic in the soil samples collected in the vicinity of the structures were higher than those in the background samples, except in the case of one structure. The concentrations in the surface soils adjacent to the 3-year-old structures seemed to be higher than those in the soils adjacent to the 8-year-old ones. Although the lateral distributions of the metals varied with the structures, chromium and arsenic appeared to show concentration gradients within 20-60 cm and 40-100 cm, respectively. Copper seemed to be the least mobile, displaying concentration gradients only within 20 cm. Even though there were no explicit vertical concentration gradients for any of the metals according to the profile soil sample analysis, chromium and copper seemed to show concentration gradients only up to 5 and 10 cm, respectively, whereas arsenic showed gradients up to 35 cm. At study sites, it was evident that heavy metals leached into soil from CCA-treated wood were confined to neighboring areas both laterally and vertically, and that any plausible ecological impact may occur only within a small range.

• Clinical and Experimental Reproductive Medicine
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• v.20 no.2
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• pp.107-115
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• 1993

Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.

• Korean Journal of Environment and Ecology
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• v.31 no.3
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• pp.279-286
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• 2017

We investigated growth response and variation of ecological niche breadth of Hibiscus hamabo according to light, moisture and nutrient when global warming is proceeded by elevated $CO_2$ concentration and temperature. H. hamabo was cultivated in experimental condition in the greenhouse that are divided by control(ambient condition) and treatment(elevated $CO_2$ concentration and temperature). Light, moisture and nutrient gradients were treated within the control and the treatment. Although H. hamabo prefers higher light intensity(up to L3) to lowers', Hamabo mallow doesn't like excessive light intensity($787{\pm}77.76{\mu}mol\;m^{-2}s^{-1}$). Also, H. hamabo was difficult to grow in absent nutrient(0%) and excessive nutrient(20%). However, there was no trend with moisture gradients. The death rate of H. hamabo in the treatment was higher in all gradients except for the highest light intensity condition than control. It means that range of tolerance about light is narrowed when concentration of $CO_2$ gas and temperature is elevated. There was no trend of death rate according to moisture gradient, comparing between control and treatment. The death rate in all nutrient gradients within the treatment is lower than the controls'. It means that range of tolerance about nutrient is widened. The ecological niche breadth of H. hamabo in the treatment was narrower as 30.1% in light gradients but wider as 8.6% in moisture gradients and 30% in nutrient gradients than in the control. In the conclusion, when global warming is proceeded by elevated $CO_2$ concentration and temperature, growth of H. hamabo would be restricted by light intensity.

• Magazine of the Korea Concrete Institute
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• v.19 no.5
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• pp.66-73
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• 2007

• Journal of the Korean Solar Energy Society
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• v.25 no.1
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• pp.79-86
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• 2005

The external conditions for estimating dynamic wind loads of wind turbines, such as the turbulence, the extreme wind, the mean velocity gradients and the flow angles, are simulated over GangWon Wind Energy Test Field placed in one of the most complex terrain in Korea. Reference meteorological data has been gathered at a height of 30m from 2003 to 2004 with a ultrasonic anemometer. The absolute value of the spectral energy are simulated and the verification of this prediction has been carried out with comparing to the experimental data. The most desirable place for constructing new wind turbine are resulted as Point 2 and Point 3 due to the lower value of Turbulence Intensity and the higher value of wind resource relatively.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.01a
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• pp.23-25
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• 2013

본 논문에서는 차세대 무인 군사 로봇에 활용할 수 있는 적군 및 아군 식별 수단으로 군복의 색깔을 이용한 기법을 제안한다. 이 기법은 전장지역의 군사로봇이 할 수 있는 피아식별법 중에 하나로 로봇에 부착되어 있는 카메라 외에 추가적으로 가져야 하는 장비가 필요 없기 때문에 추가비용 없이 효과적으로 적군을 포착할 수 있다. 군복의 색깔 차이를 식별하기 위해서는 먼저 HOG(Histogram of Oriented Gradients) 기법을 이용하여 사람을 검출한 다음, 이후 검출된 사람영역에 대하여 인체 비율을 고려해서 추출한 상의 부분의 색깔 데이터를 받는다. 이때 색공간은 HSV 공간으로 하여 조명의 변화에 덜 민감하도록 하였다. 북한 군복 색깔 영역의 pixel들만 추출하여 이진화를 한 후, 상의 전체 픽셀에 대한 개수 비율을 계산한다. 비율이 임계값 보다 높을 경우 적으로 인식한다.