• 치과방사선
• /
• 제17권1호
• /
• pp.27-49
• /
• 1987

Using ³H-proline as a radioactive tracer, the relationship between the ultrastructural differentiation and the site of protein synthesis has been investigated in developing red blood corpuscles. The general ultra-structure of erythropoietic cells in differentiation after 60 minutes of in vitro labeling has confirmed the results from previous investigations by Bessis, M., Thiery, J. and others. In dividing nuclei more than two-thirds of the labeling were present at the interface between heterochromatin and euchromatin. In less differentiated cells most of the grains in interphase cells was localized over the nucleus. As the cells continued to develope beyond a stage where cytoplasmic density was clearly increased over other cell lines in bone marrow, the majority of grains localized over the cytoplasmic area was decreased in more mature cells, as judged by the density of cytoplasm, and the structural changes in mitochondria, Golgi complex and polysomal configurations. These results show; 1) that the cytoplasm of erythroblast series does not change under in vitro conditions employed in the study; 2) that protein synthesis in the nucleus occurs largely at the interface between euchromatin and heterochromatin in active nuclei; and 3) that cytoplasmic synthesis of proteins continues to take place well into the normoblast stage solong as the physically visible polysomes are present in maturing red blood corpuscles.

• Applied Microscopy
• /
• 제20권2호
• /
• pp.12-22
• /
• 1990

The morphological characteristics and changes of the foregut in the grasshopper (Oxya sinuosa Mistshenko) have been studied with the special reference to the developmental stage. In light microscope level, the epithelia are seen the cuboidal shape in the 5th instar nymph and the adult. A number of pigment granules are appeared in the cuboidal epithelium of the 5th instar nymph, however the pigment granules were a few in the adult. Indistinct or undifferentiated folds of the epithelial layer were appeared in the 5th instar nymph, whereas the well-developed folds were in the adult. The well-developed muscular layers are seen in the 5th instar nymph, however in the adult the muscular layers are appeared thin or a few layer except the crop. In electron microscope level, in the foregut epithelium, a few round or oval shaped mitochondria, well-developed rER, Golgi complex, lysosome and a number of vacuoles were appeared in the cytoplasm. Two types of pigment granules, electron dense or homogenous and low electron dense or lamellar profiles, are seen in the esophageal epithelium. The crystalline profiles and plasma membrane infoldings were also appeared. In the foregut epithelium of the 5th instar nymph, however the cell organelles were generally undifferentiated and in the crop two types of pigment granules as the adult esophageal epithelium were also found.

• Applied Microscopy
• /
• 제26권2호
• /
• pp.137-155
• /
• 1996

The development of the ganglia of the trachea was studied by electron microscopy in human fetuses ranging from 40 mm to 260 mm crown rump length. At 40 mm fetus, the tracheal ganglia was observed in the submucosa of the trachea. The primitive ganglia consisted of neuroblasts, undifferentiated cells, and unmyelinated nerve fibers. At 50 mm fetus, the neuroblast and their processes in the tracheal ganglia ware ensheathed by the bodies or processes of satellite cells. The cytoplasm of the neuroblast contained rough endoplasmic reticulum, mitochondria, Golgi complex, and ribosomes. At 70 mm fetus, cholinergic and adrenergic axon terminals were observed. Cholinergic axon terminals with agranular vesicles were abundant in the tracheal ganglia with increasing age. During next prenatal stage from 100 mm fetus, the ganglion cells and its processes were completely covered by a thin processes of the satellite cells. Unmyelinated nerve fibers were also completely ensheathed by processes of Schwann cell. Synaptic contacts between the cholinergic axon and dendrite of ganglion cells and a few dendrodendritic synapses were first observed at 100 mm fetus. The granule-containing cells were first identified in the tracheal ganglia at 200 mm fetus. These findings indicate that tracheal ganglia of human fetus resembles other parasympathetic and sympathetic ganglia, but not the enteric ganglia.

• Applied Microscopy
• /
• 제26권2호
• /
• pp.157-175
• /
• 1996

The development of flexor digital tendon of the hand was studied by electron microscopy in human fetuses ranging from 9 mm to 260 mm crown rump length. The primordium of tendons was first identified as discrete collection of mesenchymal cells at 25 mm fetus. Synovial sheath formation had commenced by 40 mm fetus and was complete by 70 mm fetus. Cell junction or adhesion sites at all ages were noted between the tendon cells. When dilatation of the synovial cavity occurred, two types of synovial cells were observed. A-type cells had numerous vesicles and large vacuoles. In contrast, B-type cells were characterized by abundant rough endoplasmic reticulum and well-developed Golgi complex. By $150mm{\sim}260mm$ fetuses, a mojority of the synovial cells were type B. The most remarkable difference between the synovial cells of full-term fetus and adult was the larger amount of collagen fibers in the latter. The vascular buds were first observed between the individual fibril bundles in the interfascicular space at 150 mm fetus. At 25 mm fetus, collagen fibrils were first noted within narrow cytoplasmic recesses which were continued with the extracellular space. Collagen fibrils were filled in almost entire extracellular space at 150 mm fetus. Besides collagen fibrils in the extracellular space small elastic fibers were also identified and followed in their development.

• Applied Microscopy
• /
• 제24권1호
• /
• pp.77-85
• /
• 1994

In order to investigating the pulmonary toxicity of the O-chlorobenzyledene malononitrile (CS), lacrimating agent, $2.6g/m^3$ of CS was inhalated to Sprague-Dawley rats in the plastic chamber for 20 minutes. The ultrastructural changes of type II pneumocytes in the lung were observed with Hitachi 600 transmission electron microscope. The results obtained were as follows: 1. 3 hours after exposure to CS the fusion of surface microvilli, dilatation of cristernae of the rough endoplasmic reticulum, atrophy of Golgi complex and condensation, deletion of lamellated membranes in lamellar bodies were observed in type II pneumocytes. 2. One and 2 days after CS-exposure, disorganization of mitochondrial double membranes, fragmentations of rough endoplasmic reticulum were found in the great alveolar cells. In addition, decrease in amount of polyribosome granules and deletion or condensation of lamellated membranes in lamellar bodies were also observed. 3. 4 days after exposure to CS, the type II pneumocyte revealed new whorled lamellar membranes in lamellar bodies, a few intact rough endoplasmic reticulum and restoration of polyribosome granules. It is consequently suggested that CS induces degenerative changes of cytoplasmic organelles in the type II pneumocytes.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2005년도 제20차 학술대회 논문집
• /
• pp.52-52
• /
• 2005

Oogenesis, the gonadosomatic index (GSI), reproductive cycle and first sexual maturation of the female Neptunea (Barbitonia) arthritica cumingii have been investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of glycogen, lipid droplets and yolk granules. In late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. In particular, compared with the results of other gastropods, it is a different result that appearances of cortical granules at the cortical layer and microvilli on the vitelline envelope, which is associated with heterosynthetic vitellogenesis, were not observed in vitellogenic oocytes during oogenesis. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting menbrane, Monthly changes in the gonadosomatic index in females were studied in 2002 and 2003 were closely associated with ovarian developmental phases. Spawning occurred between May and August in 2002 and 2003 and the main spawning occurred between June and July when the seawater temperature rose to approximately 18${\sim}$23${\circ}$C. The female reproductive cycle can be classified into five successive stages: early activestage (Septmber to October), late active stage ( November to February), ripe stage (February to June), partially spawned stage (May to Aygust), and recovery stage (June to August).

• 한국발생생물학회지:발생과생식
• /
• 제5권2호
• /
• pp.145-150
• /
• 2001

인상어 정소는 세관형이며, 각 정소 세관은 여러 개의 정소 소낭으로 구성되어 있는데, 소낭 내의 생식 세포들은 동일한 발달 단계를 보였다. 정자형성과정 동안 소낭 세포에서는 잘 발달된 조면 소포체와 골지체가 관찰되었다. 소낭세포의 분비활성은 후기 정자변태시기에 가장 높은 것으로 나타났다. 정포 내의 정자결합물질은 정소 소낭 세포에서 분비되며, 하나의 정소 소낭에서는 하나의 정포가 만들어진다. 체외로 방출된 정포에서 피막구조는 관찰할 수 없었다. 투과전자현미경 표본에서 횡단된 하나의 정포 내에서는 1,500∼1,700개의 정자 미부가 관찰되었다.

• Applied Microscopy
• /
• 제17권1호
• /
• pp.169-176
• /
• 1987

Naturally occurring canine transmissible venereal tumors of genital organs in mature and regressive stages from 6 dogs were examined by transmission electron microscope. The tumor cells at the stage of maturation were comprised of large round and ovoid cells with prominent nuclei and nucleoli, a few spindle-shaped cells, and irregularly shaped cells. The mature round cells were characterized by the presence of a central ovoid to irregularly round nucleus with a large eccentric nucleolus, vesicular endoplasmic reticulum, round to oval swollen mitochondria with few cristae, Golgi's apparatus, and plasma membranes with numerous microvilli. As the tumor degenerated, the tumor cells were increased in the number of spindle-shaped, fibroblast-like and irregularly shaped cells, collagen bundles, and mainly lymphocytes, in contrast to those of the stage of maturation. Regressing tumor cells were characterized by the swelling and vacuolation of mitochondria and endoplasmic reticulum, membrane-bound granules, lamellar complex, tubular structures, and dense bundles of collagen. It was suggested that transformation might occur in the course of tumor growth causing morphological change from the round to the fibroblast-like cells, and that there was the evidence of cell-mediated tumor cell lysis by lymphocyte infiltration.

• 치과방사선
• /
• 제24권2호
• /
• pp.403-410
• /
• 1994

The purpose of this study was to investigate the irradiation effects on the secretory granules of the rat submandibular gland acinar cell. For this study, Sprague-Dawley strain rats were irradiated to their neck region with the dose of 5Gy and l0Gy by 6MV X-radiation, and sacrificed on the experimental periods after irradiation. The authors observed the ultrastructural changes of the secretory granules of the rat submandibular gland acinar cell under a trasmission electron microscope. The results were as follows: In the transmission electron microscopic examination, secretory granules were blurred in limiting membrane on the 3hours groups after irradiation. And they showed decrease in number, irregularities in shape and distributional pattern, and inhomogeneous internal electron density on the Iday and 3days groups. After then, these changes were recovered in shape and distributional pattern on the 14days groups, and changes of internal electron density and limiting membrane were recovered on the 28days groups after irradiation. Among the intracellular organelles, rough endoplasmic reticulum was scattered, but golgi complex was not changed. And such pathologic changes were earlier and more prominent in 10Gy irradiated groups than in 5Gy irradiated groups.

• 대한약침학회지
• /
• 제1권1호
• /
• pp.22-34
• /
• 1997

In order to investigate experimentally that effect of Moschus, Bovis Calculus, Ursi Fel aqua-acupuncture on acutely damaged liver of rats induced by radix aconiti, the author gave Moschus, Bovis Calculus, Ursi Fel aqua-acupuncture according to method of manufacture stimulation to corresponding points, Kan-su (B18) and Ki-mun (Liv14), and carried out hematological, serological examination and electromicroscopical observation. The following results were obstained : 1. At 6 hours, cell organelles induced by radix aconiti toxicity prominently destructed in control and aqua-acupuncture treatment groups. 2. At 12 hours, cell organelles, destruction was some similarities between control group and aqua-acupuncture treatment group. 3. At 24 hours, rough endoplasmic reticulum and Golgi complex comparatively developed in aqua-acupuncture group treatment as compared with control group. 4. At 48 hours, cell organelles, destruction was recovered in aqua-acupuncture treatment group as compared with the control group and it was similiar normal findings. According to the above findings, it is considered that Moschus, Bovis Calculus, Ursi Fel aqua-acupuncture has effects of recovery of acutely damaged liver.