• Journal of Animal Science and Technology
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• 제62권3호
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• pp.293-305
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• 2020

The difference in the breeding programs and population history may have diversely shaped the genomes of Korean native cattle breeds. In the absence of phenotypic data, comparisons of breeds that have been subjected to different selective pressures can aid to identify genomic regions and genes controlling qualitative and complex traits. In this study to decipher genetic variation and identify evidence of divergent selection, 3 Korean cattle breeds were genotyped using the recently developed high-density GeneSeek Genomic Profiler F250 (GGP-F250) array. The three Korean cattle breeds clustered according to their coat color phenotypes and breeding programs. The Heugu breed reliably showed smaller effective population size at all generations considered. Across the autosomal chromosomes, 113 and 83 annotated genes were identified from Hanwoo-Chikso and Hanwoo-Heugu comparisons, respectively of which 16 genes were shared between the two pairwise comparisons. The most important signals of selection were detected on bovine chromosomes 14 (24.39-25.13 Mb) and 18 (13.34-15.07 Mb), containing genes related to body size, and coat color (XKR4, LYN, PLAG1, SDR16C5, TMEM68, CDH15, MC1R, and GALNS). Some of the candidate genes are also associated with meat quality traits (ACSF3, EIF2B1, BANP, APCDD1, and GALM) and harbor quantitative trait locus (QTL) for beef production traits. Further functional analysis revealed that the candidate genes (DBI, ACSF3, HINT2, GBA2, AGPAT5, SCAP, ELP6, APOB, and RBL1) were involved in gene ontology (GO) terms relevant to meat quality including fatty acid oxidation, biosynthesis, and lipid storage. Candidate genes previously known to affect beef production and quality traits could be used in the beef cattle selection strategies.

• 한국식품과학회지
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• 제21권6호
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• pp.755-759
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• 1989

2종의 감자전분(세풍과 대지)로부터 각각 큰 입자 $(41{\mu}m$ 이상)과 작은 입자$(30{\mu}m$ 이하)를 분획하고 각 입자 크기별 가열 호화액에 대한 리올로지 특성을 분획전의 전분과 비교하면서 검토하였다. 시료 호화액은 모두 빙햄의가소성의 거동을 보였고 이 거동은 품종 또는 입자크기간의 차이를 보이지 않았으며 온도의 영향도 없었으나 농도의 증가로 의가소성의 성질은 다소 강해졌다. 점조도지수 및 항복응력값은 품종간의 차이는 없었으나 입자크기간에는 현저한 차이를 보여서 각 대응하는 농도에서 작은 입자의 경우가 큰 입자보다 훨씬 큰 점조도지수 및 항복응력값을 보였고 농도증가로 지수함수적으로 증가하였다. 또, 이들 값은 측정온도에 대하여 품종 및 입자크기에 따라 차이를 보이면서 온도증가로 감소하였고, 작은 입자>분획전의 전분입자>큰 입자의 순으로 온도의존성을 되었다.

• 대한한의학회지
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• 제19권1호
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• pp.251-270
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• 1998

The effects of Gamigunshimtang on the isolated perfused ischemic heart in rats, heart rates, left ventricular pressure, cardiac blood flow and cardiotoxicity were stu.died in H9C2 myoblast cell, myocardial slice culture The results were as follows: 1. The administration of Gamigunshimtang to the rat recovered effectively heart rate, left ventricular pressure and flow rate from the experimental ischemia in perfused rat heart. The release of lactic dehydrogenase after the ischemia also decreased compared to the control group. 2. The administration of Gamigunshimtang to H9C2 myoblast culture enhanced the cell proliferation and protected against doxorubicin and allylamine induced release of the lactic dehydrogenase into the culture medium. It also protected effectively against doxorubicin and allylamine induced decrease of Ca ATPase activity and the increase of NADPH-cytochrome C reductase activity in the microsome. 3. The administration of Gamigunshimtang to the rat myocardial slice culture protected effectively against doxorubicin and allylamine induced decreases of protein synthesis and ATP content, and increases of cvtosolic enzyme, creatin kinase into the medium and lipid peroxidation.

• Journal of Ginseng Research
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• 제41권4호
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• pp.608-614
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• 2017

Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth.

• Journal of Animal Science and Technology
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• 제56권7호
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• pp.23.1-23.7
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• 2014

Background: Scanning of the genome for selection signatures between breeds may play important role in understanding the underlie causes for observable phenotypic variations. The discovery of high density single nucleotide polymorphisms (SNPs) provide a useful starting point to perform genome-wide scan in pig populations in order to identify loci/candidate genes underlie phenotypic variation in pig breeds and facilitate genetic improvement programs. However, prior to this study genomic region under selection in commercially selected Berkshire and Korean native pig breeds has never been detected using high density SNP markers. To this end, we have genotyped 45 animals using Porcine SNP60 chip to detect selection signatures in the genome of the two breeds by using the $F_{ST}$ approach. Results: In the comparison of Berkshire and KNP breeds using the FDIST approach, a total of 1108 outlier loci (3.48%) were significantly different from zero at 99% confidence level with 870 of the outlier SNPs displaying high level of genetic differentiation ($F_{ST}{\geq}0.490$). The identified candidate genes were involved in a wide array of biological processes and molecular functions. Results revealed that 19 candidate genes were enriched in phosphate metabolism (GO: 0006796; ADCK1, ACYP1, CAMK2D, CDK13, CDK13, ERN1, GALK2, INPP1; MAK, MAP2K5, MAP3K1, MAPK14, P14KB, PIK3C3, PRKC1, PTPRK, RNASEL, THBS1, BRAF, VRK1). We have identified a set of candidate genes under selection and have known to be involved in growth, size and pork quality (CART, AGL, CF7L2, MAP2K5, DLK1, GLI3, CA3 and MC3R), ear morphology and size (HMGA2 and SOX5) stress response (ATF2, MSRB3, TMTC3 and SCAF8) and immune response (HCST and RYR1). Conclusions: Some of the genes may be used to facilitate genetic improvement programs. Our results also provide insights for better understanding of the process and influence of breed development on the pattern of genetic variations.

• 한국도로학회논문집
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• 제11권1호
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• pp.37-45
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• 2009

도로의 터널출구에서 인터체인지 분기점 구간은 도로이용자가 본선을 주행하다가 인근 목적지로 나가기 위하여 터널을 벗어나서 전방에 설치된 표지상의 목적지 지명을 확인하고, 차로를 변경해서 인터체인지 연결로로 들어가는 단계를 갖는다. 따라서 이 구간에서는 도로이용자의 다양한 행동이 요구되므로 도로이용자의 인간공학적 요소를 중시한 도로설계가 되어야 하는 구간이다. 본 연구는 도로이용자의 인간공학 요소를 고려하여 터널출구와 인터체인지 분기점구간의 최소이격거리를 제공하고자 하였다. 연구수행을 위해서 도로주행 시뮬레이터를 이용하여 다양한 연령층(20대${\sim}$70대)이 실험에 참여하여 터널과 인터체인지 분기점 산정에 대한 통계적 검증을 수행하였다. 그 결과 터널출구에서 인터체인지 변이구간(감속차로 시점)의 시점까지는 최소 500m 이상의 거리가 확보되어야 하는 것으로 나타났다. 본 연구는 과학적인 근거를 통해서 터널과 인터체인지 분기점간의 최소이격거리 정립에 대한 기초연구 수행이 이루어졌다는 점에서 논문의 의의가 있다. 향후 이용자에게 보다 안전한 지침을 제공하여 도로주행의 안전성을 도모할 수 있는 기반을 제공할 것으로 기대할 수 있다.

• 한국문헌정보학회지
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• 제16권
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• pp.71-110
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• 1989

The purpose of this study is to investigate the influential factors creating the modern library thought in the latter part of Yi Dynasty. After Korea was forced to make a treaty opening up the country in 1876, Western civilization was introduced and reforms were carried out eventually. Various influential factors could be taken into consideration as follows. (1) The envoys to Japan after 1876 (2) Tourists party to Japan organized by Gentlemen in 1982 (3) The persons who studied abroad (4) Establishment of modern schools and development of publications: on the new learning. (5) News media and others Above all, the persons who studied abroad, such as Yu Gil Jun, Yun Chi Ho, etc., in early stage has contributed most to the extension of public recognition for modern library in Korea. Therefore it can be said that the formation of modern library thought in Korea was based on the theory of the Western library as was the Japan in the period of Meiji Restoration. Patriotic intelectualls in these days believed that reform of the educational system and the diffusion of knowledge was very much meeded in order to preserve an independent Korea and to build up national strength, and then nation-wide campaign for enlightenment was earried on. For that reason the movement of the national library establishment in 1906 also should be regarded from the same point of view, that is, as a part of the enlightenment campaign. Therefore it should not go unheeded that strong patriotism was always streamed under the ground of the library campaign as well as the new learning and new education campaign. Unfortunately every plan of Korean library movement had broken down and missed the chance for having experience of self-development because of the Japanese invasion.

• 농업과학연구
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• 제46권1호
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• pp.183-194
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• 2019

Adulteration of argan oil with some other cheaper oils with similar chemical compositions has resulted in increasing demands for authenticity assurance and quality control. Fast and simple analytical techniques are thus needed for authenticity analysis of high-priced argan oil. Raman spectroscopy is a potent technique and has been extensively used for quality control and safety determination for food products In this study, Raman spectroscopy in combination with a net analyte signal (NAS)-based methodology, i.e., hybrid linear analysis method developed by Goicoechea and Olivieri in 1999 (HLA/GO), was used to predict the different concentrations of olive oil (0 - 20%) added to argan oil. Raman spectra of 90 samples were collected in a spectral range of $400-400cm^{-1}$, and calibration and validation sets were designed to evaluate the performance of the multivariate method. The results revealed a high coefficient of determination ($R^2$) value of 0.98 and a low root-mean-square error (RMSE) value of 0.41% for the calibration set, and an $R^2$ of 0.97 and RMSE of 0.36% for the validation set. Additionally, the figures of merit such as sensitivity, selectivity, limit of detection, and limit of quantification were used for further validation. The high $R^2$ and low RMSE values validate the detection ability and accuracy of the developed method and demonstrate its potential for quantitative determination of oil adulteration.

• 전기전자학회논문지
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• 제26권4호
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• pp.722-727
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• 2022

본 논문에서는 0.25 ㎛의 게이트를 갖는 AlGaN/GaN HEMT를 기반으로 개발된 X-대역 전력 증폭기 MMIC의 특성을 기술한다. 개발된 X-대역 전력 증폭기 MMIC는 9 GHz~10 GHz의 주파수 대역에서 21.6 dB 이상의 소신호 이득과 46.11dBm(40.83 W) 이상의 출력 전력을 가진다. 전력 부가 효율 특성은 43.09%~44.47%이며 칩의 크기는 3.6 mm×4.3 mm이다. 출력 전력 밀도는 2.69 W/mm²를 나타내었다. 개발된 AlGaN/GaN 전력 증폭기 MMIC는 다양한 X-대역 레이더 응용에 적용 가능하다.

• 대한중풍순환신경학회지
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• 제6권1호
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• pp.1-7
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• 2005

Objectives: Chungpyesagan-tang is a famous oriental Objectives: medicine treatment frequently used to stroke in Korea. The objective of this study is to evaluate the mechanism of the therapeutic effect of Chungpyesagan-tang on stroke in rat. Methods: We compared the difference in regional cer Methods: ebral blood flow, nitric oxide (NO) synthesis between the control and the Chungpyesagan-tang-treated group after ligating middle cerebral artery. Results: In our study, cerebral blood flow increased more in the stroke rats which had been fed by Chungpyesagan-tang than in the control. Chungpyesagan-tang induced vascular NO synthesis, but had no effect on neuronal NO synthesis. Conclusion: The mechanism of Chngpyesagan-tang's therapeutic effect on ischemic stroke could be explained by increasing cerebral blood flow and vascular NO synthesis.