• Journal of Pharmacopuncture
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• v.14 no.4
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• pp.5-11
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• 2011

Objectives: The present study was undergone to determine whether Glycyrrhizae Radix pharmacopuncture intravenous injection exerts beneficial effect against the ischemia-induced acute renal failure in rabbits. Methods: Rabbits were treated with Glycyrrhizae Radix pharmacopuncture via i.v., followed by renal ischemia/reperfusion. The fractional excretion of glucose and phosphate were measured and the malondialdehyde content was also determined. The morphological changes of cortical part of kidney also observed with light microscope. Results: Renal ischemia/reperfusion caused increase of the fractional excretion of glucose and phosphate in ischemia-induced animals, which was prevented by Radix Glycyrrhizae extract treatment. Ischemia/reperfusion increased lipid peroxidation, which was prevented and morphological changes also altered by Radix Glycyrrhizae pharmacopuncture administration. Conclusions: These results indicate that lipid peroxidation plays a critical role in ischemia-induced acute renal failure and Glycyrrhizae Radix pharmacopuncture exerts the protective effect against acute renal failure induced by renal ischemia/reperfusion.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.73-83
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• 2011

Objectives : The purpose of this study is to investigate anti-allergic effects of Glycyrrhizae Radix(GR) pharmacopuncture and GR extract. Methods : In vivo, animals were gotten GR pharmacopunctures at both sides of ST36s three times for 5 days. Then, we investigated anti-DNP IgE-induced passive cutaneous anaphylaxis of Sprague Dawley rats. In vitro, we measured cell viability, ${\beta}$-hexosaminidase release, and the secretion of interleukin-4(IL-4) and tumor necrosis factor-alpha(TNF-${\alpha}$) in RBL-2H3 cells after treatment of various concentrations of GR extract. Results : In vivo, we observed inhibition of passive cutaneous anaphylaxis after GR pharmacopuncture treatments at both sides of ST36s and optional points. In vitro, GR extract treatments did not affect cell viability, but inhibited ${\beta}$-hexosaminidase release and the secretion of IL-4 and TNF-${\alpha}$. Conclusions : These results suggest that GR pharmacopuncture and GR extract should be beneficial in the inhibition of allergic inflammatory response.

• Korean Journal of Oriental Medicine
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• v.12 no.3 s.18
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• pp.79-90
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• 2006

This study was investigated to determine the quality monitoring of distributed materials from Glycyrrhizae Radix (26 samples), Atractylodis Rhizoma Alba (24 samples) according to storage period after $1{\sim}3$ year. We have estimated by identification, purity, loss on drying, ash, acid insoluble ash, extract content, essential oil content, assay and microbial contamination. As a result, Glycyrrhizae Radix (26 samples) were satisfied with the standard of K.P. (Korean Pharmacopoeia) and WHO's microbial contamination limit standard. In the Atractylodis Rhizoma Alba (24 samples), 2 samples were not satisfied with the standard of K.P.(Korean Pharmacopoeia) and WHO's microbial contamination limit standard. The results make practical application of the basic data for the quality control of herbal medicine in storage.

• Herbal Formula Science
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• v.22 no.1
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• pp.105-112
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• 2014

Background and objective: Methamphetamine (Meth) is a widely abused psychostimulant that produces hyperlocomotion in rodents. Radix of Glycyrrhizae uralensis comprises a variety of bioactive components that have neuroprotective effects. In a previous study, we have demonstrated methanol extracts from radix of Glycyrrhizae uralensis (MEGR) suppress acute cocaine-induced extracellular dopamine release in the nucleus accumbens. In the present study, we investigated the effect of MEGR on acute Meth-induced hyperlocomotion. Methods: Male Sprague-Dawley rats were orally administered with MEGR (60 mg/kg and 180 mg/kg) 60 min prior to an intraperitoneal injection of Meth (1.0 mg/kg). Results: Behavioral analysis showed acute Meth greatly increased locomotor activities, while pretreatment with MEGR dose dependently inhibited the hyperlocomotion. In parallel, there were markedly increased levels of dopamine and its metabolite 3, 4-dihydroxyphenylacetic acid in the nucleus accumbens tissues in Meth-treated rats, which were also almost completely reversed by 180 mg/kg MEGR. Conclusions: These results showed that radix of Glycyrrhizae uralensis attenuates Meth-induced hyperlocomotion by inhibiting dopamine synthesis and utilization, suggesting that radix of Glycyrrhizae uralensis might be effective in blocking the rewarding effect of Meth.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.126-126
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• 2018

Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.143-153
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• 2004

Antimicrobial activities against Streptococcus mutans, Candida albicans, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus were assayed through the hot water extracts and the ethanol extracts from Chungyulsodokeum and its composition oriental medicines. The stains were incubated on culture medium and activated. We observed the size of inhibition zone on the strains that is incubated in strilized filter paper disc with various concentration extracts of Chungyulsodokeum and its composition 1. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, Gardeniae Fructus showed antimicrobial activities against Staphylococcus aureus. 2. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Forsythiae Fructus Ghycyrrhizae Radix, showed antimiaobial activities against Staphylococcus epidermidis. 3. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, showed antimicrobial activities against Streptococcus mutans. 4. The extract of Coptidis Rhizoma showed antimicrobial activities against Candida albicans. 5. None of the extracts showed antimicrobial activities against Escherichia coli. We observed antimicrobial activities of Chungyulsodokeum and its composition against Streptococcus mutans, Staphylococcus epidermidis, Staphylococcus aureus.

• Journal of Korean Medicine Rehabilitation
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• v.28 no.3
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• pp.39-53
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• 2018

Objectives The aim of this study was to analyze the herbal prescription and composition of the leading experimental studies of herbal medicine extract in arthritis model and to analyze the way of setting model and the way of validating effect of the leading experimental studies of herbal medicine extract in arthritis model. Methods The Subjects were selected by select criteria and exclusion criteria from January 1, 2000 to September 30, 2017. The herbal prescription and composition of the selected subjects were analyzed. The effects and statistical significance of selected studies were verified by comparing the control and treatment groups of frequently used cytokines (tumor necrosis factor $[TNF]-{\alpha}$, interleukin $[IL]-1{\beta}$, IL-6). Results 52 kinds of herbal prescriptions and 118 kinds of individual herbs were used in 64 studies. Among the 52 kinds of herbal prescriptions, Daeganghwal-tang (4 times) was used most frequently. Among the 118 kinds of individual herbs, Glycyrrhizae Radix (49 times), Angelicae Giantis Radix (34 times), Osterici Radix (24 times) were used most frequently in order. The prescription that has the highest $TNF-{\alpha}$ decline is Changchooldoin-tang. The prescription that has the highest $IL-1{\beta}$ decline is Banggibokryeong-tang. The prescription that has the highest IL-6 decline is Gwanjeol8ho-bang. Conclusions Daeganghwal-tang (herbal prescription) and Glycyrrhizae Radix, Angelicae Giantis Radix, Osterici Radix (individual herbs) were used most frequently. The prescriptions that have the highest cytokines decline were Changchooldoin-tang, Banggibokryeong-tang and Gwanjeol8ho-bang.

• Fashion & Textile Research Journal
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• v.12 no.5
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• pp.667-675
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• 2010

This research did chistosan and cation treatment to raise K/S vlue. In this result, chitosan 1%, cation 4% treatment showed the highest K/S value. Gilycyrrhizae radix dyeing showed the highest K/S value in pH3, pre-mordant, $30^{\circ}C$, 80 min.. Its color Y, YR. K/S value over 2.5 times showed in chitosan treatment fabrics, 5 times in cation treatment fabrics than cotton fabrics on the condition of none-mordant ones. In the antibacterial activity, staphylococus showed 99.9% in all fabrics. Klebsilla Pneumonia also showed 97.1% in chitosan treatment fabrics and it showed 99.9% in other all fabrics. In the deodorization and UV-cut effect, the more K/S value and the larger effect was showed.

• The Journal of Korean Medicine
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• v.30 no.2
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• pp.79-87
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• 2009

Objective: This study assessed five respective herbs in Cheonggugamrosu (CGGRS) for ability to inhibit malodor in vitro. Methods: We used malodor modeling of the salivary sediment system. Incubation mixtures were prepared with sediment at 16.7% (v/v), amino acids (cysteine and tryptophan) at 6mM and either herb extract at 1% (w/v) or water as control. Mixtures were incubated at $37^{\circ}C$ and odor was monitored organoleptically and for volatile sulfur compounds with the Halimeter. Indole/skatole was determined by Kovac's colorimetric method. Results: Two of the herbs, Caryophilli Flos and Glycyrrhizae Radix, had inhibitory effect on malodor generation. Both of them significantly reduced VSC, organoleptic odor and indole/skatole formation (p<0.05). Conclusions: We found Caryophilli Flos and Glycyrrhizae Radix played a main role in CGGRS. Both herbs have potential as effective anti-malodor agents and this suggests they are worthy of further exploration.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.6
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• pp.1053-1058
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• 2010

Glycyrrhizae radix (GR) is an herbal medicine commonly used in East Asia for treating a variety of diseases, including stomach disorders. In this study, the antidepressant-like activity of GR was investigated using the forced swimming test (FST) in rats. After the FST, the expression of c-Fos and corticotrophin releasing factor (CRF) was assessed by immunochemistry of brain samples from the paraventricular nucleus of the hypothalamus (PVN). The results of the FST showed that a high dose (400 mg/kg) of extract was very effective in reducing immobility(P<0.01), and increased climbing. In addition, treatment with GR (400 mg/kg) significantly decreased the expression of c-Fos and CRF in the PVN, compared to controls. In conclusion, the findings of this study demonstrated that GR effectively reduced behavioral and physiological depression responses in an animal model of depression, suggesting that GR might be useful in the treatment of clinical depression.