• Title/Summary/Keyword: Glutamic Acid

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Characteristics of Polysaccharide Extracted from the Cultured Mycelia of Schizophyllum commune (치마버섯 균사체 배양물로부터 분리한 다당류의 이화학적 특성)

  • Lee, June-Woo;Kim, Yong-Seok
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.91-95
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    • 2009
  • To examine physico-chemical properties of the polysaccharide extracted from liquid-cultured mycelia of Schizophyllum commune, each the polysaccharide was extracted with hot water treatment and then fractionated with ethanol, alkaline solution and ultrafiltration. And we determined carbohydrate contents, composition of amino acids, infra-red spectrum and viscosity. Carbohydrate contents of polysaccharide treated with ethanol and ultrafiltration were 72.0% and 62.3%, and proteins content were 15.3% and 32.0% respectively. The carbohydrate consisted of four monosaccharides and the protein contained 16 amino acids. The polysaccharide obtained from ultrafiltration was shown an absorption band characteristic of the ${\beta}$-glycosidic linkage by infra red spectra. These results suggest that the polysaccharide extracted from Schizophyllum commune showed the characteristics of proteinbounded polysaccharide, and it was ${\beta}$-glycosidic linkage with strong viscosity.

쑥 첨가가 돈육제품의 아미노산 및 지방산 조성에 미치는 영향

  • Gang, Se-Ju;Hyeon, Jae-Seok;Jeong, In-Cheol;Mun, Yun-Hui
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2004.05a
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    • pp.123-126
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    • 2004
  • 소시지의 구성아미노산 총량은 Bo 소시지가 Eo 소시지보다 많고, B+ 소시지가 E+ 소시지보다 많아서 원료육의 등급이 소시지의 구성아미노산 총량에 영향을 미치는 것으로 판단된다. 패티의 아미노산 함량은 Bo 패티가 Eo 패티보다 총량이 적었고, 쑥을 첨가한 패티가 첨가하지 않은 패티보다 많았다. 수육의 아미노산 총량은 Bo 수육이 Eo 수육보다 적어서 원료육의 등급에 따른 차이가 있었다. 햄의 아미노산 총량은 Bo 햄이 Eo 햄보다 적어서 등급에 의한 차이가 있었다. 네 종류의 육제품 모두에서 가장 많은 조성을 차지하는 아미노산은 glutamic acid, aspartic acid, lysine, leucine, arginine, alanine의 순이었으며, 필수아미노산은 5.45${\sim}$13.60% 사이었다. 돈육 소시지의 지방산 조성은 포화지방산이 34.44${\sim}$35.94%, 불포화지방산이 60.71${\sim}$63.72%로 등급이나 쑥의 첨가에 의한 영향은 없었다. 패티의 지방산 조성은 전체적으로 포화지방산이 33.83${\sim}$37.14% 수준이고, 불포화지방산은 59.52${\sim}$63.09%로 Bo 패티가 Eo 패티보다 높았으며, 쑥의 첨가에 의한 차이는 크지 않았다. 수육의 지방산 조성은 포화지방산이 40.87${\sim}$45.73%로 Bo와 Eo 수육이 비슷하여 등급에 의한 영향은 없었다. 그리고 쑥을 첨가한 B+와 E+수육이 쑥을 첨가하지 않은 Bo와 Eo 수육보다 포화지방산 조성비가 적제 나타났다. 햄의 포화지방산은 쑥을 첨가하지 않고 제조한 Bo 햄이 Eo 햄보다 다소 많았고, 쑥을 첨가하지 않은 햄이 쑥을 첨가한 햄보다 적었다.분산시키는 것으로 나타났다. 또한 고기를 단계별 복합처리로 저 allergy 처리는 단계별로 점차적으로 allergy가 감소되었다. 즉 단계별로 억제가 안되는 것부터 억제되는 처리를 복합적으로 처리한 것으로 그 단계는 천연효소처리에 인산염 처리, 여기에 초음파 처리, 마지막 단계로 3분 끓이면 억제율이 68%까지 억제되었다. 이는 단일처리시 전혀 억제를 못하는 처리를 단계별로 한 단계씩 더해가면 allergy 억제효과가 나타난다고 할 수 있겠다. 초음파 처리도 역시 저 allergy 처리 공정에 이용될 수 있는데 이것은 그 처리로 인해 새로운 알러젠이 생성될 수도 있다. 또한 복합처리로 allergy를 감소시키면 연속적이고 동시적으로 하기 때문에 원가를 절감할 수 있다.환경현안에 대한 정치경제적 접근을 외면하지 말고 교과서 저작의 소재로 삼을 수 있어야 하며, 이는 '환경관리주의'와 '녹색소비'에 머물러 있는 '환경 지식교육'과 실천을 한단계 진전시키는 작업으로 이어질 것이다. 이후 10년의 환경교육은 바로 '생태적 합리성'과 '환경정의'라는 두 '화두'에 터하여 세워져야 한다.배액에서 약해를 보였으나, 25% 야자지방산의 경우 50 ${\sim}$ 100배액 어디에서도 액해를 보이지 않았다. 별도로 적용한 시험에서, 토마토의 경우에도 25% 야자지방산 비누 50 ${\sim}$ 100배액 모두 약해를 발생하지 않았으나, 오이에서는 25% 야자지방산 비누 100배액에도 약해를 나타내었다. 12. 이상의 결과, 천연지방산을 이용하여 유기농업에 허용되는 각종의 살충비누를 제조할 수 있었으며, 방제가 조사결과 진딧물, 응애 등 껍질이 연약한 곤충의 방제에 효과적인 것으로 나타났다. 따라서 제조된 살충비누를 활용하면 환경친화적인 해충방제가 가능하다고 판단되었다.능을 충진시켜

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Processing of Fermented Squeezed-type Pen Shell By-product by Proteolytic Enzyme (효소분해법에 의한 키조개부산물 젓갈의 제조)

  • KANG Hoon-I;KANG Tae-Joong;BAE Tae-Jin;KIM Hyun-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.509-514
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    • 1994
  • A processing method for fermented paste of favorable flavor and texture using pen shell by-product was investigated. The pen shell by-product was homogenized with the addition of water and hydrolyzed with $5\%$ of Protin P(105 PU/g) at $55^{\circ}C$ for 1 hour. Flavor of the hydrolysate could be improved by thermal treatment at $100^{\circ}C$ for 40 minutes with $10\%$ of invert sugar. $2\%$ of agar-agar and $6\%$ of starch added to hydrolysate emulsified by $8\%$ of polyglycerol condensed ricinoleate(PGDR) were significantly effective for the improvement of rheological properties such as hardness, springiness and chewiness of the fermented paste. $15\%$ of table salt was finally added to the product of fermented pen shell paste. The contents of moisture, protein, lipid, carbohydrate and salinity of the product were $62.7\%,\;3.2\%,\;4.4\%,\;10.6\%\;and\;15.6\%$, respectively. The major free amino acids were glutamic acid, arginine, aspartic acid, leucine, lysine, glycine and alanine. The product was stable for the storage of 60 days at $23{\pm}3^{\circ}C$ on bacterial growth.

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감귤과피 유색미의 취반 특성

  • 서성수;노홍균;윤광섭;김순동
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.157-157
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    • 2003
  • 우리나라에서 생산되는 감귤은 지리적 기후 조건으로 내한성이 강한 만다린계 온주밀감이 주종을 이루나 최근 과잉생산으로 인한 가격하락과 수입자유화에 따른 대처의 일환으로 경쟁력이 높은 품종을 장려 보급하고 있다. 감귤의 국내 연간 생산량은 56만 톤으로 과일 전체 생산량의 약 30%를 차지하며 과실의 약 20%가 과피로서 그 일부가 한약재로 쓰이나 대부분이 버려지고 있다. 감귤의 과피에는 carotenoids, bioflavonoids, pectin 및 terpenes가 풍부하게 함유되어 있으며, 천연에서 발견되고 있는 약 300여종의 carotenoids계 색소 중 115종이 감귤에 존재한다. 감귤 과피의 주요 carotenoids로는 비타민 A의 역할을 하는 $\beta$-carotene과 cryptoxanthin을 비롯한 $\beta$-citraurin이며 천연 착색제로 활용되고 있다. 또, 주요 bioflavonoids로는 모세혈관의 수축을 촉진시켜 고혈압 예방과 이로 인한 각종 질환을 방지하는 작용을 가진 hesperidin과 혈액내 LDL 콜레스테롤의 양을 줄이는 작용이 알려진 naringin이 있다. 그 외의 감귤 flavonoids도 항산화작용, antimutagen 활성, 항암, 항알레르기 및 항바이러스 효과가 알려져 있다. 밀감 과피의 bioflavonoids는 약 60여종이 분리되어 그 구조가 밝혀져 있으나 90% 이상이 hesperidin이다. 또 과피유에는 $\delta$-limonene을 주성분으로 하는 휘발성 향미성분이 함유되어 있어 향미 개선제로서의 활용이 기대되고 있다. 본 연구는 최근 식생활의 서구화로 쌀 위주의 식생활에 많은 변화를 가져와 쌀 소비량은 해마다 줄어들고 있는 반면 기능성을 가미한 쌀의 소비가 늘고 있음을 감안하여, 우리나라 제주도에서 생산되고 있는 감귤 과피의 물 균질액을 쌀에 코팅하여 아름다운 색상과 기능성을 지닌 유색미를 제조함과 동시에 그 취반 특성을 조사하였다. 그 결과 유색미의 색상은 진노랑(L*; 63.6, a*; -7.87, b*; 46.35)으로 취반 후에도 유사한 색상을 나타내었다. Total carotenoids는 감귤과피에서는 10.74mg%, 과피의 물 균질액에는 0.46mg%이었으며 유색미에는 0.l2mg%, 유색미 취반에는 0.05mg%를 나타내었다. Hesperidin은 과피, 물 균질액, 유색미 및 유색미 취반에서 각각 2173.l2mg%, 108.65mg%, 21.73mg% 및 8.67mg%이었으며, naringin은 각각 1468.40mg%, 73.38mg%, 14.62mg% 및 5.87mg%를 나타내었다. 감귤과피의 유리아미노산은 asparagine이 94.22mg%로 가장 높았으며, methionine이 24.88mg%, alanine이 19.64mg%, ${\gamma}$ -aminoisobutylic acid가 15.37mg%로 이들 4종의 아미노산이 전체 유리아미노산함량의 70%를 나타내었다. 유색미 취반에는 백미 취반에 비하여 총 유리아미노산의 함량이 15%정도 증가되었으며, 그 중에서 glutamic acid는 2.5 배가 증가되었다. 감귤과피의 주요 무기질은 K(652.60mg%)와 Ca(222.50mg%)로 전체 무기질 함량의 86%를 차지하였으며 유색미 취반은 백미 취반에 비하여 K는 2.3배, Mn는 76%, Ca, P, Mg은 16~26%, Fe는 13%가 각각 증가되었다. 취반의 경도, 점착성 및 깨짐성은 유색미 취반과 백미 취반간에 차이가 없으나 응집성과 탄력성은 유색미 취반에서 높았다. 색상에 대한 기호도, 구수한 맛 및 종합적인 기호도는 유색미 취반에서 높았으며 단맛, 쓴맛은 백미 취반과 차이를 보이지 않았다.

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Effects of Perilla Oil on the Levels of Plasma Lipids and Other Biochemical Paramaters in Rats (들깨기름을 투여한 흰쥐의 혈액 지질량과 그외 혈액성분 변화 고찰)

  • 서화중
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.703-709
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    • 2001
  • To compare the hypolipidemic effects of perilla oil with different kinds of dietary fat and oil forty eight 20 days old male Wistar rats were fed one of the following diets for 4 weeks: basal diet for control containing 9.4 w/w% corn oil(CO), 9.4 w/w% beef tallow (BO), 9.4 w/w% perilla oil(PO) and 4.7 w/w% beef tallow plus 4.7% perilla oil(BP). The amount of diet consumed and body weight gain rate were not significantly different among the four dietary groups. The levels of plasma triglyceride and total cholesterol in PO group were significantly lower than those of BO and BP groups. PO group also had significantly lower LDL-cholesterol in BP group were than other groups. The levels of plasma triglyceride tatal-cholesterol and LDL-cholesterol in BP group were significantly lower than those in BO group by 9.2%, 10.3% and 18.6% respectively. Plasma glutamic oxaloacetic transferase and alkaline phosphatase activities and uric acid levels in PO group were significantly higher than other groups and were somewhat beyond the normal levels. These findings showed that perilla oil with hypolicpidemic effects could have some adverse effects on hepatic and other organic functions in rats.

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The Quality Characteristics of Beef Jerky According to the Kinds of Saccharides and the Concentrations of Green Tea Powder (당의 종류와 녹차가루 첨가량에 따른 육포의 품질특성)

  • 박금순;이선주;정외숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.2
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    • pp.230-235
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    • 2002
  • The purpose of this study was to investigate the characteristics of beef jerky adding of many kinds of saccharides and three levels of green tea powder. Color and hardness were increased with the addition of green tea powder while tenderness was decreased. Moistureness Gf beef jerky added honey was higher than that of sugar, oligo saccharide. In color, the lightness was decreased with increasing concentrations of green tea Powder. Redness and yellowness values were increased. In measurement of texture, hardness were increased with the addition of green tea powder. The hardness of beef jerky by adding of honey appeared to be higher than that of sugar, oligo saccharide. Gumminess and brittleness were increased with the addition of green tea powder while springness was decreased. Springness in addition of 3% green tea powder was higher than control. The beef jerky added with 3% green tea powder and sugar had good overall perference.

Nutritional Evaluation for Head, Feet and Tails Tissue of Pig (돼지머리, 족발, 꼬리의 영양학적(營養學的) 연구(硏究))

  • Ryu, Beoung-Ho;Kim, Hee-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.149-155
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    • 1984
  • Raw and cooked pig's head, feet and tails were evaluated for yield, proximate composition, caloric value, and the contents of amino acids, minerals and vitamins. The yields of the raw pig's head, feet and tails were 67.4%, 46.1% and 67.6%, respectively. Corresponding values for the cooked were 54.1%, 34.3% and 47.6% respectively. No significant differences were observed for proximate composition between these samples and any other source3 of the meats. The high energy values, 253.8-310.5 Cal/100 g, of the samples were largely due to the fat content. Among the essential amino acids, these products showed a higher lysine, leucine and threonine content, and lower methionine and tryptophan content. The total amounts of non-essential amino acids were more than half of those of all amino acids. Neither the raw nor the cooked pig's head, feet and tails contained vitamin A. On these data, pig's head, feet and tails tissue have a relatively low in vitamin B content as compared to conventional pork cuts.

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Studies on Selective Modulators and Anti-anorexigenic Agents in Korean Red Ginseng (한, 일 고려인삼 심포지움)

  • Hiromichi Okuda;Keizo Sekiya;Hiroshi Masuno;Takeshi Takaku;Kenji Kameda
    • Journal of Ginseng Research
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    • v.11 no.2
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    • pp.145-252
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    • 1987
  • Isolated rat adipocytes are well known to possess opposite pathways of lipid metabolism: lipolysis and ipogenesis. Both of the metabolism respond to various biologically active substances such as epinephrine, ACTH and insulin. Epinephrine and ACTH stimulate lipolysis and insulin accelerates lipogenesis. Recently, Korean red ginseng powder was found to contain adenosine and an acidic poptide which inhibited epinephrine-induced lipolysis and sl imulated insulin-mediated lipogenesis from added glucose. The acidic peptide is consisted mainly of glutamic acid and glucose. Ginsenosides Rb1 and Re inhibited ACTH-induced lipolysis in isolated rat adipocytes, while they did not affect insulinstimulated lipogenesis, Thus, all these substances extracted from Korean red ginseng exhibited selective modulations toward the opposite metabolic pathways in rat adipocyte; They inhibited the lipolysis but not the lipogenesis. We call these substances"selective modulators". Recently, we isolated a toxic substance named "toxohormone-L " from ascites fluid of patients with various malignant tumors. The toxohormone-L stimulated lipolysis in rat adipocytes and induced anorexia in rats. Both the lipolytic and the anorexigenic actions of toxohormone-L were found to be inhibited by ginsenoside Rb2 in Korean red ginseng. Based on these results, physiological signifi¬cances of these substances in Korean red ginseng were discussed. Pan ax ginseng is a medicinal plant long used in treatment of various pathological states including general complaints such as head ache, shoulder ache, chilly constitution and anorexia in cancer patients, There have been many pharmacological studies on Panax ginseng roots. Petkovllreported that oral administration of an aqueous alcoholic extract of ginseng roots decreased the blood sugar levtl of rabbits. Saito2lreported that Panax ginseng suppressed hyperglycemia induced by epinephrine and high carbohydrate diets. These findings suggest that Panax ginseng roots contain insulin-like substances. Previously, we demonstrated that gin¬seng roots contain an insulin-like peptide which inhibits epinephrine-induced lipolysis and stimulated insulin-mediated lipogenesis. In 1984, we suggested that such an insulin-like substance should be called a selective modulator4). Present investigation describes the details of the selective modulators in ginseng roots. During progressive weight loss in patients with various neoplastic disease, depletion of fat stores have been observed. The depletion of body fat during growth of neoplasms is associated with increase in plasma free fatty acids. Recently, we found that the ascites fluid from patients with hepatoma or ovarian tumor and the pleural fluid from patients with malignant lymphoma elicited fatty acid release in slices of rat adipose tissue in vitro. The lipolytic factor, named"toxohormone-L". was purifed from the ascites fluid of patients with hepatoma. The isolated preparation gave a single band on both disc gel electrophoresis and sodium dodecyl sulfate(SDS)-acrylamide gel electrophoresis in the presence of ${\beta}$-mercaptoethanol. Its molecular weight was determined to be 70,000-75,000 and 65,000 by SDS-acrylamide gel electrophoresis and analytical ultracentrifugation, respectively. Injection of toxohormone-L into the lateral ventricle of rats significantly suppressed food and water intakes. There was at least 5 hr delay between its injection and appearance of its suppressive effect. In the present study, we also tried to find a inhibitory substance toward toxohormone-L from root powder of ginseng.

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Taste Compounds and Functionality of Flavoring Substances from Small Kingfish (소형 갈전갱이틀 이용한 풍미소재의 정미성분)

  • Oh, Kwang-Soo;Moon, Soo-Kyung;Heu, Min-Soo
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1345-1349
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    • 1998
  • The flavor constituents and functionality of small kingfish were studied as affected by two stage enzyme hydrolysis (TSEH). Total free amino acid contents in water extract, autolytic extract and TSEH of small kingfish were 541.3 mg%, 8,245.3 mg% and 6,636.6 mg%, respectively. Major free amino acids in TSEH were hydroxyproline, glutamic acid, proline, leucine, phenylalanine, lysine, arginine. As for nucleotides and other bases, IMP, TMAO and total creatinine were principal components in TSEH. And the major inorganic ions in TSEH were Na, K, P, Cl and $PO_{4}$. Also TSEH of small kingfish revealed very higher Angiotensin-I converting enzyme inhibition effect than those of water and autolytic extract.

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Screening of Lactic Acid Bacteria for Strong Folate Synthesis and Optimization of Fermentation (고엽산 생산능의 유산균 탐색 및 발효 조건 최적화)

  • Du, Kyung Min;Park, Se Jin;Park, Myung Soo;Ji, Geun Eog
    • Korean Journal of Food Science and Technology
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    • v.46 no.3
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    • pp.328-333
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    • 2014
  • Folate is a water-soluble vitamin B that is required for the synthesis of amino acids and nucleic acids. It plays an important role in cell division and cell growth in several living organisms. The purpose of this study was to screen strong folate-synthesizing bacteria and to optimize their culture conditions for folate production. Folate production was quantified by microbiological assays by using folate-dependent strain Lactobacillus rhamnosus KCTC 3237. Folate derivatives were identified by LC-MS/MS. Of the 65 strains of bifidobacteria and lactobacilli tested, L. plantarum Fol 708 demonstrated the greatest ability to produce folate. Its optimal pH for folate production was 5.5 in a pH-controlled, lab-scale fermenter. Coculturing L. plantarum Fol 708 with L. brevis GABA 100 in a milk medium enhanced the level of folate produced in comparison to culturing L. plantarum Fol 708 alone.