• Archives of Pharmacal Research
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• 제27권10호
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• pp.1023-1028
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• 2004

A new 12a-dehydrorotenoid 1, 11-dihydroxy-9, 10-methylenedioxy-12a-dehydrorotenoid (1), together with a new isoflavonoid glycoside tectorigenin-7-O-${\beta}$-glucosyl-4'-O-${\beta}$-glucoside (3), were isolated and identified from the rhizomes of I. spuria (Zeal). In addition, 4 known compounds, tectorigenin (2) tectorigenin-7-O-${\beta}$-glucosyl $(1{\leftrightarrow}6)$ glucoside (4), tectoridin (a tectorigenin- 7-O-${\beta}$-glucoside) (5) and tectorigenin-4'-O-${\beta}$-glucoside (6) were isolated and identified for the first time from this plant. The structures of the isolated compounds were determined by spectroscopic methods (UV, IR, $^1H,\;^{13}C$NMR, DEPT, HMQC, NOESY, and HMBC experiments and MS spectrometry) and by comparison with literature data of known compounds. Compounds 2, 4, 5, and 6 are reported for the first time from this plant through the present study.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.579-587
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• 2018

For biotechnological production of high-valued ${\beta}-{\text\tiny{D}}$-hexyl glucoside, the catalytic properties of Hanseniaspora thailandica BC9 ${\beta}$-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside was optimized. The constitutive BC9 ${\beta}$-glucosidase exhibited maximum specific activity at pH 6.0 and $40^{\circ}C$, and the activity of BC9 ${\beta}$-glucosidase was not significantly inhibited by various metal ions. BC9 ${\beta}$-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 ${\beta}$-glucosidase exhibited enhanced production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside in the presence of DMSO, and 62% of ${\beta}-{\text\tiny{D}}$-hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.

• Food Science and Biotechnology
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• 제14권6호
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• pp.778-782
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• 2005

Analysis of black soybeans [Glycine max (L.) Merr.; 59 Korean varieties] revealed that 100-seed weights of green cotyledon seeds (33.5 g, n=31) were higher than those of yellow ones (28.9 g, n=28). Contents of delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), petunidin-3-glucoside (P3G), and total anthocyanins in seed coats of black soybeans were 0.03-4.15, 0.74-18.36, 0.02-1.60, and 0.87-23.52 mg/g, respectively, among which most prominent anthocyanin was C3G (80.9% of total content), followed by D3G (13.6%) and P3G (5.5%). No significant differences were observed in color parameters $a^*$ and $b^*$ between black soybeans with yellow cotyledon (BYC) and green cotyledon (BGC). Total isoflavone content of BGC was higher than that of BYC, and negative correlation was found between total anthocyanin and isoflavone contents.

• 한국약용작물학회지
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• 제16권4호
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• pp.231-237
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• 2008

본 연구는 염전에서 자라고 있는 퉁퉁마디를 채집하여 플라보노이드 성분을 LC/MS로 정량하였다. 퉁퉁마디 분말에서 flavonol계 물질인 quercetin (124.43 ppm), rutin (2.57 ppm), quercetin-3-${\beta}$-glucoside (3992.49 ppm), quercetin-3',4'-glucoside(0.08 ppm) 그리고 isorhamnetin (27.81 ppm)이 검출되었다. 특히 quercetin-3-${\beta}$-glucoside가 차지하는 비중은 건초에서 99%, 분말에서 96% 이상으로 높게 나타났다. 이러한 결과들은 염생식물인 퉁퉁마디가 항산화제로서의 높은 기능성 물질 임을 시사해 주고 있다.

• 한국잠사곤충학회지
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• 제45권1호
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• pp.1-5
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• 2003

최근 안토시아닌 색소의 생리활성에 대한 관심이 고조됨에 따라 천연색소 자원으로서 유망시되는 뽕나무 오디의 이용성을 높이고 생리활성물질로서 C3G(cyanidin-3-glucoside) 색소를 다량 함유한 뽕나무의 품종육성을 위하여 유전자원으로 보존되고 있는 뽕 계통에 대하여 오디를 수확한 후 색소를 추출, 분리 및 정량 분석을 실시하였다. 1. 오디 함유 anthocyanin 색소의 추출은 1% HCl-MeOH 용액을 사용하였으며, 분석 기기로는 Shimadzu LC, Nova-pack $C_{18}$ Column(300${\times}$3.9 mm)을 사용하였다. 2. 오디와 포도에 존재하는 안토시아닌 색소의 조성 패턴을 비교한 결과, 오디는 C3G(cyanidin-3-glucoside) 단일 물질로 존재하는 반면, 포도는 여러 가지 안토시아닌 색소가 혼합되어 존재하는 것으로 나타났다. 3. 오디와 포도의 계통별 C3G(cyanidin-3-glucoside) 함량을 분석한 결과, 포도에 비해 오디의 C3G함량이 상대적으로 매우 높았다. 4. 이상의 결과 뽕나무 열매인 오디는 안토시아닌 색소의 존재형태, 이용부위 및 함량에 있어 천연색소 자원으로서 유망시되며, 색소자원으로 이용시 타작물보다 유리한 경제수종으로 판단된다.

• Journal of Nutrition and Health
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• 제30권4호
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• pp.425-433
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• 1997

Levels and distribution of five B-6 vitamers(PMP, PM, PLP, PL, and PN) and pyridoxine $\beta$-glucoside conjugates(PN-glucoside) were examined in milk of American women who received supplements of 2.5 or 10mg PN HCl/d and of unsupplemented Egyptian women during the first six months of lactation. B-6 vitamer and PN-glucoside levels in human milk were determined by reverse-phase HPLC. Pyridoxal(PL), which has been reported to be the most rapidly absorbed form of vitamin B-6 and may facilitate bioavailability, was the predominant vitamer in human milk of all three groups. Pyridoxal made up 72% of total vitamin B-6 for the 2.5mg supplemented group, 76% for the 10mg group, and 59% for the Egyptian group. Level and Percent PL were significantly lower for Egyptian women. Mean growth of the two American groups was similar to each other and within the normal range of the NCHS reference, however, Egyptian infants showed growth faltering at 6 months. The Percent of PN-glucoside, a less bioavailable form of vitamin B-6 in humans was 1% in milk of American women and was 11% in Egyptian women and these values were significantly different. for Egyptian women, total vitamin B-6 levels in breast milk correlated Positively with animal protein intake(r=0.91) and percent PN-glucosides(r=0.53) and negatively with plant protein intake(r=-0.55). These findings showed that high plant protein intake was associated with low concentrations of PL and total vitamin B-6 in human milk.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.285-290
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• 2021

Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases. Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids in S. herbacea that are known to exert various pharmacological activities. Therefore, our study sought to validate and optimize an HPLC/UV-based analytical method for I3G and Q3G yield quantification, as well as to determine its limit of detection, limit of quantification, linearity, precision, and accuracy. Upon testing a concentration range of 31.5-1.9 ㎍/mL the results exhibited good linearity (r² ≥0.9996 and r² ≥0.9999 for I3G and Q3G, respectively), and the procedure was deemed precise (relative standard deviation of ≤3.19 and ≤3.85%, respectively), and accurate (102.6-105.0 and 92.9-95.2%, respectively). The results showed that our proposed method could be used for rapid I3G and Q3G evaluation in S. herbacea.

• 한국식품과학회지
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• 제31권3호
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• pp.815-822
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• 1999

우리 나라 전역에 자생하는 쑥(Artemisia vulgaris)의 전초의 수용성 에탄올추출물로 부터 21가지의 flavonoids를 분리하였다. 이들 화합물의 동정은 H-NMR, mass, UV-스펙트럼을 이용하여 동정하였으며, 동정된 flavonoids들은 tricin, jaceosidine, eupafolin, diosmetin, chrysoeriol, humoeridictyol, isorhamnetin, apigenin, eriodictyol, luteolin, luteolin 7-glucoside, kaempferol 3-glucoside, kaempferol 7-glucoside, kaempferol 3-rhamnoside, kaempferol 3-rutinoside, quercetin, quercetin 3-glucoside, quercetin 3-galactoside, quercetin, quercetin 7-glucoside, rutin 그리고 vitexin으로 동정 되었다. 이들 분리된 각 flavonoids들에 대하여 쥐의 간에서 추출한 마이크로좀에 대하여 지질과산화 효과를 살펴보았다. 이들 flavonoids화합물들의 항산화효과는 비타민 E와 비교하였을 때 높은 활성이 나타났다. 이미 강력한 항산화물질로 잘 알려진 quercetin, apigenin, eriodictyol등의 화합물의 $IC_{50}$ 값은 각각 0.9, 0.3, $0.3\;{\mu}g/mL$로 나타났으며, methoxylated flavonoids인 eupafolin, jaceosidine, diosmetin 등의 화합물도 $IC_{50}$ 값이 1.0, 1.4, $1.0\;{\mu}g/mL$로 나타나 비타민 E에 비교할 때 높은 활성을 나타냈다.

• Applied Biological Chemistry
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• 제50권3호
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• pp.238-243
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• 2007

Limonoid계 화합물인 limonin, nomilin, limonin glucoside의 함량을 당유자 과실의 성숙시기별 조직부위별로 liquid chromatography-mass spectrometry(LC-MS)를 이용하여 정량하였다. Nomilin과 limonin의 함량은 6월 미성숙기부터 증가하였으며 nomilin은 10월 성숙기, limonin은 12월 성숙후기에서 그 함량이 최고치를 나타냈으며, 두 가지 화합물 모두 3월 완숙후기에는 함량이 현저하게 감소하였다. 이와 대조적으로 limonin glucoside 함량은 10월 성숙기부터 완숙 후기에 이르기까지 지속적으로 증가하였다. 세 가지 성분 모두 과실이 완숙함에 따라 peel에서의 함량은 감소하는 경향을 보인 반면, juice sac에서의 함량은 증가하는 경향을 보였다. 완숙후기에 채취한 당유자 과실의 조직부위별 limonin, nomilin 및 limonin glucoside의 함량을 분석한 결과, limonin과 nomilin은 씨앗에서 함량이 가장 높게 나타났고, 다음으로는 segment membrane가 높게 나타났다. 반면 limonin glucoside는 segment membrane에서 함량이 가장 높은 것으로 확인되었으며, 그 다음은 juice sac에서 함량이 높게 나타났다.

• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.614-618
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• 2014

Resveratrol, which is a polyphenolic antioxidant, is dose-dependent when used to provide health benefits, to enhance stress resistance, and to extend lifespans. However, even though resveratrol has therapeutic benefits, its clinical therapeutic effect is limited owing to its low oral bioavailability. An Escherichia coli system was developed that contains an artificial biosynthetic pathway that produces resveratrol glucoside derivatives, such as resveratrol-3-Oglucoside (piceid) and resveratrol-4'-O-glucoside (resveratroloside), from simple carbon sources. This artificial biosynthetic pathway contains a glycosyltransferase addition (YjiC from Bacillus) with resveratrol biosynthetic genes. The produced glucoside compounds were verified through the presence of a product peak(s) and also through LC/MS analyses. The strategy used in this research demonstrates the first harnessing of E. coli for de novo synthesis of resveratrol glucoside derivatives from a simple sugar medium.