• 한국식품영양과학회지
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• 제33권9호
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• pp.1419-1425
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• 2004

본 연구는 이팝나무 잎의 용매분획물을 이용하여 DPPH 소거 작용, 환원력과 같은 항산화 활성과 tyrosinase 저해 활성을 실험한 결과 에틸 아세테이트 분획물에서 높은 항산화 및 tyrosinase 저해 활성을 나타내었다. 따라서 silica gel column chromatography와 MPLC를 이용하여 그 활성물질을 분리하였으며, 분리한 화합물은 $^1H$, $^{13}C$-NMR 및 2DNMR을 이용하여 luteolin-4'-O-glucoside로 구조동정을 하였다. 분리한 luteolin-4'-O-glucoside와 기존 항산화제와의 비교실험에서 DPPH 라디칼 소거 활성과 환원력은 시료의 농도가 증가함에 따라 활성이 증가하였다. 순수하게 분리한 luteolin-4'-O-glucoside를 이용하여 tyrosinase 저해 활성도 농도 의존적이었으며, $IC_{50}$ 값은 23.2 ${\mu}g/mL$로 나타났다. 그러므로 luteolin-4'-O-glucoside는 식품첨가물로 항산화제로서의 활용도 및 미백효과를 지니는 기능성 화장품원료로서 이용가치가 높을 것으로 생각된다.

• Nutrition Research and Practice
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• 제13권6호
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• pp.473-479
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• 2019

BACKGROUND/OBJECTIVES: Anti-inflammatory and antioxidative activities of luteolin and luteolin-7-O-glucoside were compared in galactosamine (GalN)/lipopolysaccharide (LPS)-induced hepatitic ICR mice. MATERIALS/METHODS: Male ICR mice (6 weeks old) were divided into 4 groups: normal control, GalN/LPS, luteolin, and luteolin-7-O-glucoside groups. The latter two groups were administered luteolin or luteolin-7-O-glucoside (50 mg/kg BW) daily by gavage for 3 weeks after which hepatitis was induced by intraperitoneal injection of GalN and LPS (1 g/kg BW and $10{\mu}g/kg\;BW$, respectively). RESULTS: GalN/LPS produced acute hepatic injury by a sharp increase in serum AST, ALT, and $TNF-{\alpha}$ levels, increases that were ameliorated in the experimental groups. In addition, markedly increased expressions of cyclooxygenase (COX)-2 and its transcription factors, nuclear factor $(NF)-{\kappa}B$ and activator protein (AP)-1, were also significantly attenuated in the experimental groups. Compared to luteolin-7-O-glucoside, luteolin more potently ameliorated the levels of inflammatory mediators. Phase II enzymes levels and NF-E2 p45-related factor (Nrf)-2 activation that were decreased by GalN/LPS were increased by luteolin and luteolin-7-O-glucoside administration. In addition, compared to luteolin, luteolin-7-O-glucoside acted as a more potent inducer of changes in phase II enzymes. Liver histopathology results were consistent with the mediator and enzyme results. CONCLUSION: Luteolin and luteolin-7-O-glucoside protect against GalN/LPS-induced hepatotoxicity through the regulation of inflammatory mediators and phase II enzymes.

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.2938-2940
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• 2011

Novel C-aryl glucoside SGLT2 inhibitors containing triazine motif were designed and synthesized for biological evaluation. Among the compounds assayed, triazine containing methoxy moiety 18 demonstrated the best in vitro inhibitory activity against hSGLT2 in this series to date ($IC_{50}$ = 24.9 nM).

• 생약학회지
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• 제24권3호
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• pp.244-246
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• 1993

From the herb of Oenanthe javanica Dc. (Umbelliferae) ${\beta}-sitosteryl$ glucoside, stigmasteryl glucoside, isorhametin and hyperin were isolated and identified by chemical and spectral analysis.

• Applied Biological Chemistry
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• 제22권1호
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• pp.39-41
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• 1979

우리나라 재래종(在來種)갓의 anthocyanin 색소(色素)의 구명(究明)을 위(爲)한 실험(實驗)에서 제일보(第一報)의 구조추정(構造推定)에 이어서 이들 색소(色素)의 함량(含量)을 측정(測定)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 채취시기(採取時刻)의 구별(區別)에 의한 전(全) anthocyanin의 함량(含量)은 peonidin-3-glucoside로 표시(表示)하여 봄갓이 175.5mg% 가을갓이 192.7mg%로 가을갓이 약간(若干) 더 많은 함량(含量)을 보였다. 2, 착색정도(着色程度)의 구별(區別)에 의한 전(全) anthocyanin의 함량(含量)은 peonidin-3-glucoside로 표시(表示)하여 잎의 양면착색(兩面着色)갓이 290.2mg%, 한면(面)만의 착색(着色)이 73.6mg%, 양면(兩面)모두 무착색(無着色)갓이 40.0mg%이었다. 3. 신선(新鮮)한 갓에서 peonidin-3-glucoside로 표시(表示)하여 40mg%이하의 anthocyanin 함량(含量)으로는 육안(肉眼)으로서는 전혀 갓특유(特有)의 착색(着色)을 감지(感知)할 수 없었다. 4. 갓에 존재(存在)하는 이종(二種)의 anthocyanin의 조성비(組成比)는 채취시기(採取時期)및 착색(着色)의 정도(程度)에 따라서도 거의 일치(一致)하였으며 그 조성(組成)은 peonidin-3-glucoside가 55.3% 그리고 peonidin-3-galactoside가 44.7%이었다.

• 생약학회지
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• 제47권3호
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• pp.211-216
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• 2016

In the course of our continuing search for biologically active components from Korean medicinal plants, we isolated the main compound, luteolin 5-glucoside from aqueous fraction of Ajuga spectabilis. The structure was elucidated by the basis of $^1H$ and $^{13}C$ NMR and TOF ESI-MS data. Quantitative analysis of luteolin 5-glucoside was carried out on a XBridge C18 column ($S-5{\mu}m$, $4.6{\times}250mm$) with gradient elution composed of acetonitrile:water. The results exhibit that the average content of main compound in A. spectabilis were 0.048%. Oxidative stress plays a major role Alzheimer's disease (AD) and other neurodogenerative disease. AD is major health problem and there is currently no clinically accepted treatment to cure or stop its progression. Pretreatment with luteolin 5-glucoside markedly attenuated $H_2O_2$-induced cell viability loss in a dose-dependent manner. Luteolin 5-glucoside also inhibited the formation of intracellular reactive oxygen species in SH-SY5Y. The results suggest that luteolin 5-glucoside from A. spectabilis has protective effects against oxidative stress-induced cytotoxicity, which might be a potential therapeutic compound for treating and/or preventing neurodegenerative disease implicated with oxidative stress.

• 한국식품과학회지
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• 제12권4호
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• pp.305-312
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• 1980

우리나라에서 처음 재배(栽培)를 시작한 elderberry anthocyanin의 화학구조(化學構造)를 동정하고 함량(含量)을 측정(測定)하였다. Elderberry열매에서 1% HCl MeOH로 추출(抽出)하여 얻은 색소농축액(色素濃縮液)을 Amberlite IRC-50 양(陽)이온교환수지관을 통과시켜 정제(精製)하고 paper chromatography로 개별색소(個別色素)를 분리(分離)하였다. 각(各) 개별색소(個別色素)에 대해 부분산가수분해(部分酸加水分解), acyl group, 결합당(結合糖), aglycone의 확인(確認), paper chromatography로 얻은 $R_f$값, spectral data 등을 기초로 구조를 확인(確認)하였다. 확인(確認)된 elderberry중(中)의 5가지 색소(色素)는 cyanidin-3-glucoside, cyanidin-3,5-diglucoside, cyanidin-3,5-diglucoside with p-coumaric arid, cyanidin-3-xyloglucoside-5-glucoside, cyanidin-3-xyloglucoside-5-glucoside with p-coumaric acid이었다. 총 anthocyanin양은 elderberry 1g당 3.13mg이고 개별색소(個別色素)의 비(比)는 cyanidin-3-glucoside 4.3%, cyanidin-3,5-diglucoside 28.3%, cyanidin-3,5-diglucoside with p-coumaric arid 12.9%, cyanidin-3-xyloglucoside-5-glucoside 38.3%, cyanidin-3-xyloglucoside-5-glucoside with p-coumaric acid가 16.2%이었다.

• Archives of Pharmacal Research
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• 제14권1호
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• pp.52-54
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• 1991

A new acylated monoterpene glucoside, galloylpaeoniflorin, was isolated from Paeony root. The structure was determined by chemical and spectroscopic methods.

• Natural Product Sciences
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• 제12권2호
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• pp.89-93
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• 2006

One new sterol glucoside, $gomphsterol\;{\beta}-D-glucoside$ 1 along with known compounds, ${\beta}-sitosterol$, stigmasterol, campesterol, $stigmasterol-{\beta}-D-glucoside$, friedelin, 3-epi-friedelinol, allantoin, and $chrysoeriol-7-O-{\beta}-D-glucoside$ have been isolated from the aerial parts of Gomphrena globosa (Amaranthaceae). On the basis of spectroscopic (including 2D NMR) and chemical studies, the structure of 1 was elucidated as $(22E,24S)-24-ethylcholesta-7,9(11),22-trien-3{\beta}-ol-3-O-{\beta}-D-glucopyranoside$. Known compounds are reported for the first time from this plant species.

• 한국식품영양과학회지
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• 제29권1호
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• pp.106-110
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• 2000

We investigated the active oxygen scavenging activity and active compound from Humulus japonicus. The ethyl acetate and butanol fraction exhibited strong scavenging effects on hydroxyl radical. Furthermore active compound was isolated and purified using Amberlite XAD-2 column chromatography and preparative HPLC from ethyl acetate fracton, and major compound was identified as a luteolin-7-O-$\beta$-D-glucoside by the MS, UV, 1H-NMR and 13C-NMR analyses. Luteolin-7-O-$\beta$-D-glucoside exhibited strong scavenging effect on active oxygens such as superoxide radical, hydroxyl radical and hydrogen peroxide.