• Tuberculosis and Respiratory Diseases
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• 제69권2호
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• pp.81-94
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• 2010

Background: Autophagy is an important adaptive mechanism in normal development and in response to changing environmental stimuli in cancer. Previous papers have reported that different types of cancer underwent autophagy to obtain amino acids as energy source of dying cells in nutrient-deprived conditions. However, whether or not autophagy in the process of lung cancer causes death or survival is controversial. Therefore in this study, we investigated whether nutrient deprivation induces autophagy in human H460 lung cancer cells. Methods: H460, lung cancer cells were incubated in RPMI 1640 medium, and the starved media, which are BME and RPMI media without serum, including 2-deoxyl-D-glucose according to time dependence. To evaluate the viability and find out the mechanism of cell death under nutrient-deprived conditions, the MTT assay and flow cytometry were done and analyzed the apoptotic and autophagic related proteins. It is also measured the development of acidic vascular organelles by acridine orange. Results: The nutrient-deprived cancer cell is relatively sensitive to cell death rather than normal nutrition. Massive cytoplasmic vacuolization was seen under nutrient-deprived conditions. Autophagic vacuoles were visible at approximately 12 h and as time ran out, vacuoles became larger and denser with the increasing number of vacuoles. In addition, the proportion of acridine orange stain-positive cells increased according to time dependence. Localization of GFP-LC3 in cytoplasm and expression of LC-3II and Beclin 1 were increased according to time dependence on nutrient-deprived cells. Conclusion: Nutrient deprivation induces cell death through autophagy in H460 lung cancer cells.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.407-415
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• 2017

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)-related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: $32.5{\pm}10.1%$ vs control: $77.8{\pm}5.3%$). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, $200{\mu}M$) and melatonin ($0.1{\mu}M$), in BIP/GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, $p-eIF2{\alpha}$, $eIF2{\alpha}$, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.

• 한국치위생학회지
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• 제20권3호
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• pp.313-324
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• 2020

Objectives: The aim of this study was to confirm the association among the health status, health behaviors, and periodontitis according to total, age and sex in cases of adult metabolic syndrome(MetS). Methods: This cross-sectional study used collected data from the 7^th Korea National Health and Nutrition Examination Survey (KNHANES) conducted from 2016 to 2018 with 3,394 adults with MetS aged 19-79 years. The complex samples logistic regression analysis confirmed the relevant factors for periodontitis. Results:Periodontitis was diagnosed in 43.6% of all MetS cases. Diabetes (Odds Ratio [OR]=1.554), abdominal obesity (OR=1.336), current smoking (OR=2.465), past smoking (OR=1.379), and not-using oral care products (OR=1.414) were associated with periodontitis in MetS. In the age of 19-39 years with MetS group, diabetes (OR=5.379), elevated blood pressure (OR=3.975), current smoking (OR=7.430), and not using oral care products (OR=3.356) were associated with periodontitis. In the 40-79 age group, diabetes (OR=1.398), abdominal obesity (OR=1.360), current smoking (OR=2.022), and not using oral care products (OR=1.416) were associated with periodontitis. In the male MetS group, current smoking (OR=3.119), past smoking (OR=1.625), and brushing teeth more than three times (OR=0.743) were associated with periodontitis. In the female MetS group, diabetes (OR=1.733), impaired fasting glucose (OR=1.434), abdominal obesity (OR=1.479), and not using oral care products (OR=1.992) were associated with periodontitis. Conclusions: Improvement in blood sugar control, obesity, smoking cessation, and oral health education, including how to use oral care products in all individuals with MetS may result in improved oral health. In addition, improvement in elevated blood pressure in the MetS group aged under 40 years, and brushing teeth more than three times a day in the male MetS group can reduce the risk of periodontitis. Therefore, public and oral health professionals should emphasize on the relationship between age and sex during the metabolic syndrome management program and share relevant information with patients.

• Applied Biological Chemistry
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• 제38권3호
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• pp.278-282
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• 1995

오미자 추출물이 Saccharomyces cerevisiae의 생리에 미치는 영향을 조사하기 위하여 포도당 기본배지에 오미자 추출물을 0, 0.01, 0.1, 0.5, 1%(w/v)씩 첨가하고 S. cerevisiae KTCC 1199를 접종하여 96시간 진탕배양하면서 균의 증식, 알콜생성, 발효율 등의 변화와 alcohol dehydrogenase, pyruvate decarboxylase활성을 조사하였다. S. cerevisiae의 증식은 전 발효기간중 추출물 0.01%및 0.1%첨가구에서 대조구보다 증가하였으며, 각 시험구의 알콜생성은 추출물 0.1%, 0.01%, 0%, 0.5%, 1% 첨가순으로 0.1%첨가구에서 가장 높았다. alcohol dehydrogenase 효소활성은 0.1%구와 0.01%구에서 대조구보다 각각 1.25배, 1.18배 높은 활성을 보였다. pyruvate decarboxylase 활성의 경우도 ADH 활성과 유사하여 각각 대조구보다 1.31배, 1.26배 높은 활성을 보였으며, 0.5%구와 1%구는 대조구보다 각각 1.3배및 1.4배 낮은 활성을 보였다.

• Applied Biological Chemistry
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• 제23권4호
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• pp.228-241
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• 1980

홍삼(紅蔘)의 ethanol추출물(抽出物)과 crude saponin, panaxadiol 및 triol계(系) 사포닌이 주정효모(酒精酵母) Saccharomyces cerevisiae IAM와 Saccharomyces formosensis No. 396 IAM의 생리(生理)에 미치는 영향을 조사하기 위하여 Hayduck 10% glucose 배양액(培養液)에 홍삼성분(紅蔘成分)을 $0{\sim}8.0%$의 농도(濃度)로 첨가(添加)하고 $30^{\circ}C$에서 120시간(120時間) 정치배양하면서 그 효과(效果)를 조사하였다. 효모(酵母)의 세포수(細胞數), 주성분(酒精分)의 생산량(生産量) 및 당(糖)의 소비율(消費率)가 발효율(醱酵率)은 2균주(2菌株) 모두가 홍삼(紅蔘)의 추출성분첨가전구(抽出成分添加全區)에서 무첨가구(無添加區)에 비(比)하여 증가(增加)되었고 ethanol 추출물(抽出物)은 1,5%첨가(添加)에서 사포닌은 Sacch. cerevisiae가 PD, Sacch. formosensis는 PT첨가(添加)에서 각각(各各) 최대(最大)의 증가(增加)를 나타내었다. $CO_2$의 발생량(發生量)에서도 같은 경향(傾向)으로 ethanol추출물(抽出物) 1.5%첨가(添加)에서 가장 빠르고 초대치(最大値)를 보였고 그이하(以下) 또는 그이상(以上)에서는 오히려 발효가 억제되었다. pH는 발효가 진행됨에 따라 서서히 저하되었다. 효모처리 발효는 첨가 홍삼추출성분(紅蔘抽出成分)의 사포닌패턴조성(組成)에 거의 영향을 주지않았으나 $ginsenoside-Rg_2$는 약간 증가되고 $ginsenoside-Rd$는 약간 감소되는 경향으로 2균주(菌株) 모두에서 나타났다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제9권3호
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• pp.49-55
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• 2004

염화에틸렌 화합물에 오염된 토양으로부터 고농도 (150mg/L)의 PCE를 cis-DCE까지 탈염소화하는 혼합미생물 농화 배양계 LYF-1을 구축하였다. LYF-1은 효모추출물, 펩톤, 포름산, 아세트산, 락트산, 피루브산, 시트르산, 석신산, 글루코오스, 수크로오스, 에탄올 등을 전자공여체로 이용하여 PCE를 탈염소화할 수 있었다. 한편, PCE를 대신할 수 있는 전자 수용체에 의한 PCE 탈염소화에 미치는 영향을 살펴본 결과, NO$_3$$^{-}$와 NO$_2$$^{-}$는 PCE의 탈염소화반응을 완전히 저해하였으나, S$_2$O$_3$$^{-2}$ , SO$_3$$^{-2}$ 및 SO$_4$$^{-2}$ 는 PCE의 탈염소화반응에 그다지 큰 영향을 미치지 않았다. LYF-1 혼합미생물을 혐기성 고정생물막 반응기내의 세라믹 메디아에 부착하고, PCE의 유입부하율 변화에 따른 처리 효율을 조사한 결과, PCE의 부하율 0.13-0.78 $\mu$moles/L/hr의 범위 내에서 99% 이상의 PCE 탈염소화 효율을 보였으며, PCE 탈염소화 반응의 최종산물은 cis-DCE이었다.

• 한국약용작물학회지
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• 제26권4호
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• pp.271-280
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• 2018

Background: Astragalus membranaceus is a well known oriental medicinal herb. The polysaccharides of the aboveground parts (AMA) and the radix (AMR) of A. membranaceus are the most important functional constituents. Methods and Results: The aim of this study was to determine the effects of AMA and AMR on the oxidative damage induced in the skeletal muscle of rats subjected to exhaustive exercise. Sprague-Dawley rats were randomly divided into exercise and non-exercise groups; in the groups receiving the test compounds, AMA and AMR were administered orally for 30 days. Skeletal muscle samples were collected from each rat after running to exhaustion on a treadmill to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and the concentation of malondialdehyde (MDA). The antioxidant enzyme activities of SOD and GSH-Px of skeletal muscle of AMA- and AMR-treated groups were significantly higher than those of the control and commercial sports drink (SPD)-treated groups in exhaustive exercise rats. In addition, MDA concentrations in the skeletal muscle of the AMA- and AMR-treated groups were significantly lower than those of the control and SPD-treated groups. In the present study, the effects of AMA and AMR on exercise endurance capacity were also evaluated in mice subjected to a swimming exercise test. AMA and AMR supplementation prolonged the swimming time of mice and enhanced exercise endurance capacity. AMA and AMR possess the ability to retard and lower the production of blood lactate, and prevent the decrease of serum blood glucose. Conclusions: These results showed that, AMR and AMA exerted beneficial effect in mice, increasing the activity of the antioxidant systems and inhibiting oxidative stress induced by exhaustive exercise. The compounds improved exercise performance and showed anti-fatigue effects against exhaustive exercise.

• 동의생리병리학회지
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• 제31권5호
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• pp.264-269
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• 2017

Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin-induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ${\leq}300mg/dL$. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.

• Biomolecules & Therapeutics
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• 제11권4호
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• pp.257-264
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• 2003

Carnitine, hydroxycitric acid, and soy peptide have been known to be anti-obesity agents. The purpose of this study was to evaluate the combined effects of carnitine, hydroxycitric acid, and soy peptide mixture as a potential anti-obesity supplement in overweight women. Overweight premenopausal women (n=33; PIBW>110; 20 to 39 years) were randomized into two groups: the placebo group and the functional beverage group (the test group). Functional beverage was composed of 2000 mg soy peptide, 20 mg L-carnitine and 300 mg garcinia(40% hydroxycitric acid). Body weight and 3 day food dimes, biochemical measurements and computerized tomography were measured at baseline and 8-week. After 8-week consumption of functional beverage with usual diet and exercise, body weight fell an average of 1.4 kg (2.1%). Visceral fat area reduced an average of 7.8% at L1($69.6{\pm}8.7\;vs\;64.2{\pm}7.5\;\textrm{cm}^2$) and 5.1% ($60.7{\pm}4.9\;vs\;57.6{\pm}4.8\;\textrm{cm}^2$, p<0.05) at L4level after weight loss in the test group. Calf fat area in the test group showed about 10% reduction ($31.0{\pm}2.7\;vs=\;27.7{\pm}1.7\;\textrm{cm}^2$, p<0.05) after weight loss. These reductions in fat areas were not shown in the placebo group. There were tendencies of increase in serum levels of $\beta-hydroxybutyrate$, acetoacetate, and total ketones in the test group. There were 7% and 17% insignificant increase in fasting free fatty acid (FFA) and response area of FFA during oral glucose tolerance test(OGTT), respectively, in this group. ill addition, little weight loss in the test group showed 8% but not significant reduction in insulin response area during OGTT. In conclusion, this study shows that taking a mixture of carnitine, hydroxycitric acid, and soy peptide as a potential anti-obesity supplement for 8-week produced advantageous changes in the weight and visceral fat accumulation of overweight women.

• 생명과학회지
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• 제23권2호
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• pp.190-196
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• 2013

멍게의 물렁증 발병으로 인해 멍게 양식에 커다란 타격을 받고 있는 가운데 물렁증 발병에 대한 분자적인 접근을 위해 정상 멍게와 물렁증 걸린 멍게에서 DEG 법을 수행하였다. 이번 연구에서 멍게의 당 생합성 효소인 PGK가 물렁증에 걸린 멍게 개체에서 발현이 감소하는 것을 다양한 실험을 통해서 확인하였다. PGK에 대한 유전자 서열을 확보함과 동시에 이 유전자의 발현에 영향을 미치는 부위인 프로모터를 클로닝 하였다. 프로모터 부위의 단일 염기 다형성 분석을 통해서 -106과 -254 위치의 염기에서 다형성이 일어나는 것을 확인하였다. 물렁증에 대한 저항성을 가진 멍게와 다른 개체군의 멍게와의 염기서열을 비교한 결과 많은 차이가 나타남을 확인하였다. 이러한 염기의 차이가 PGK의 발현에 영향을 미치는 지 확인하기 위해서 각각의 멍게 개체군에서 genomic DNA와 total RNA를 분리하여 genotyping과 RT-PCR을 수행하였다. 그 결과 -106과 -254 위치의 염기가 AA와 GT인 개체에서 PGK의 발현량이 상대적으로 많은 것을 확인할 수 있었다. 이러한 결과는 물렁증에 대한 저항성을 가진 멍게를 선발육종하기 위한 분자적인 마커의 개발에 활용됨으로써 물렁증 발병을 줄일 수 있음을 시사하였다.