• 한국패류학회지
• /
• 제23권2호
• /
• pp.181-187
• /
• 2007

A scanning electron microscopic study on the glochidium and glochidial encystment of Anodonta arcaeformis on the host fish Carassius auratus was conducted. The shape of the glochidium was apparently subtriangular and its average size was $270\;{\mu}m\;\times\;260\;{\mu}m\;\times\;145\;{\mu}m$. The glochidial shell valves were of the same size, kept together by a ligament that is 50.4 ${\mu}m$ in length and 5.5 ${\mu}m$ in width. Each of the glochidial shell valve had a long hook studded with many spines on the superior face. A large area of at the apex of the valve surrounding the base of the hook was provided with numerous small spines which became progressively smaller toward the periphery of the area. The glochidial shell valve consisted of two layers. The mantle cells line the glochidial shell valves and some of hair cells were observed. A larval thread was 2.3 ${\mu}m$ in diameter. In the artificial infection of the glochidia to one of the natural hosts, Carassius auratus, it took about three to four hours to encyst the glochidia with epithelial cells of the fish fins. The encystment method was the cell migration from the neighboring epithelial cells.

• 한국패류학회지
• /
• 제5권1호
• /
• pp.1-9
• /
• 1989

A scanning electron microscopic study on the glochidium and glchidial encystment of Anodonta grandis on the guppy was conducted. The shape of the glochidium is apparently triangular and its averge size is 0.45mm X0.4mm when closed, The two glochidial shell valves are of the same size, kept together by a ligament of 120${\mu}{\textrm}{m}$ in length and 7 ${\mu}{\textrm}{m}$ in width. Each of the glochidial shell valves has a 16 ${\mu}{\textrm}{m}$ long hook sitdded with many spines on the superior face. A large area to the apex of the valve surrounding the base of the hook is provided with numerous small spines which become progressively smaller towards the periphery of the area, The external surface of the glochidial shell valve is covered with numerous small processes showing successive change in the shape and the pattern of destribution by part. Besides the processes, there are a number of niches scattered all over the exterior surface. The glochidial shell valve has two layers. One is the outer thin membrane bearing the processes and the niches and the others is the inner layer bearing numerous holes which any accessory structure and 2.65 ${\mu}{\textrm}{m}$ in diameter, emerges from a canal located at center of ventral plate of the mamtle, A total of three types of the hair cells are observed. In present artificial infection of the glochidium to the guppy, it took about three to four hours to complete an early cysts, During the period of encystment, The epithelial cells of the host fish actively migrated toward the attached glochidium and covered it.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.595-596
• /
• 2001

A scanning electron microscopic study on the glochidium and glochidial encystment of Anodonta archefomis on the host fish Carassius auratus was conducted. The shape of the glochidium is apparently subtriangular and its average size is 270${\mu}{\textrm}{m}$ $\times$ 260${\mu}{\textrm}{m}$ $\times$ 145${\mu}{\textrm}{m}$. The glochidial shell valves are of the same size, kept together by a ligament of 50.4${\mu}{\textrm}{m}$ in length and 5.5${\mu}{\textrm}{m}$ in width. (omitted)

• 한국패류학회지
• /
• 제7권1호
• /
• pp.76-86
• /
• 1991

A scanning electron microscopic stuey on the glochidial encystment study on the golchidal encystment and excystment of Anodonta fukudai on Acheilognathus yamatsutae, a common natural hostfish, was conducted. The glochidium easily attached to the unscaled surfaces of the host fish such as the fins, lips, and the wall of the buccal cavity. For this study, the fins infected with the glochidia wer mainly observed in a series. The process of encystment was slowly progressed, for 21-25 hours for the early cyst and for 2-4 days for the thick walled cyst. The process of excystmint was visually detected on the 12th day since the attachmint was occurred. The first visible sign was a little tear of the cyst wall covering the hinge and marginal zones of the juvenile clam and once the little sign was appeared the progress of emerging and dettachmint of the juvenile clam from the host was finished relatively in short time. During the process of the encystmint, the cells participationg in covering the attached glochidirm were seened mainly supplied by migration from the surroundings. the shapes of the cells migrating and covering the glochidium were considerably changed and the surface structures of the cells lost their normal pattern of the surface ridges. The unstable forms of the cells were observed almost all throughout the period of the glochidial attachment. No cells of the host epithelium, which were still attached to the juvenile clam energing from the cyst, were observed. The most juvenile clams escaped from the cysts were a little bigger than the glochidia and they were still possessed of the golchidial hooks even though much degenerated. The first growth line was appeared on the shell valves of the juvenild clam when observed right after dettachment.

• 한국발생생물학회지:발생과생식
• /
• 제2권1호
• /
• pp.81-87
• /
• 1998

한국산 작은대칭이 (Anodonta arcaeformis flavotincta)의 유생발생 중 유생(glochidium)을 숙주어류인 붕어(carassius auratus)에 감염시켜 유생의 피낭형성과정(부착부위, 부착후 탈락가지의 시기)을 알아보기 위해, 주사전자현미경을 이용하여 관찰한 결과 다음과 같은 결론을 얻었다. 유생들은 30분내에 숙주어류의 니트러미, 구강 그리고 아가미에 부착되었다. 이 연구에서 유생이 부착된 숙주어류의 지느러미를 시간별로 관찰하였다. 유생의 부착률은 숙주어류의 가슴지느러미,꼬리지느러미, 배지느러미에 각각 30%, 22%, 17%였다. 피낭형성과정은 천천히 진행되었다. 1차 피낭형성은 24시간에서 27시간째에 이루어졌고, 그리고 5일 내지 6일째 유생은 숙주조직의 상피세포로 완전히 덮였다. 유패의 탈락과정은 숙주에 감염시킨후 8일째에 관찰되었다. 유생의 대부분은 15일 이내에 숙주어류의 피낭으로부터 탈락되었다. 유생이 숙주에 부착되기 전과 숙주어류의 피낭으로부터 탈락된 유패의 크기는 변화가 없었다.