• Journal of agriculture & life science
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• v.43 no.6
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• pp.95-103
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• 2009

To develop natural intermediate flavoring substances, optimal hydrolysis conditions and taste compounds for two step enzyme hydrolysate(TSEH) using Grass puffer(Takifugu niphobles) were investigated. The optimal conditions for TSEH method were revealed in temperature at $55^{\circ}C$ 3 hour digestion with Alcalase (pH 7.5) at the 1st step and 2 hour at $45^{\circ}C$ digestion with Flavourzyme(pH 6.0~6.5) at the 2nd step. TSEH method was superior to hot-water extraction on the aspect of yield, nitrogen contents and organoleptic taste quality such as umami and control of bitter taste formation. In taste active-components in Glass puffer TSEH, total free amino acid content was 4,502 mg%, major free amino acids were Pro, Leu, Lys, Hypro, Tau, Arg, Phe, Ala, Glu and Val in ordor. As for nucleotides, IMP(372 mg%) was the principal component and also contents of TMAO, total creatinine, and betaine were 43, 278 and 41 mg% in Glass puffer TSEH, respectively. The major inorganic ions in TSEH were Na(949 mg%), K(222 mg%), Cl(1,180 mg%) and $PO_4$(1,081 mg%).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.1
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• pp.124-128
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• 2021

This study was conducted to evaluate the effects of incubation methods and transfer timings on the hatching rate of fertilized eggs of the river puffer Takifugu obscurus. Four incubation methods were tested, a) control (fertilized eggs attached to the glass plate), b) bottom (fertilized eggs spread on the bottom of the tank without any treatment), c) S-bottom (removing the stickiness of the fertilized eggs, and then spreading the eggs on the bottom of the tank), and d) incubator (removing the stickiness of the fertilized eggs, and then incubating the eggs in an incubator). Additionally, four transfer timings were tested: a) control (no transfer from the incubation tank), b) zygote (fertilized eggs transferred at the zygote stage), c) segmentation (fertilized eggs transferred at the segmentation stage), and d) pharygula (fertilized eggs transferred at the pharygula state). The results showed that the hatching rate of incubator was significantly higher than those of control, bottom, and glass (P<0.05). The results also showed that the hatching rates of control and pharygula were significantly higher than those of zygote and segmentation (P<0.05).

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.986-990
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• 2003

The living Glass puffers, Takifugu niphobles (Bogseom as Korean name), 10 specimens, were purchased at Tongyeong traditional fisheries market every month from November, 2001 to October, 2002, and tested anatomical distribution of the toxicity such as muscle, skin, intestine, liver and ovary by mouse bioassay method. Significant seasonal variations of the toxicity were not shown in muscle, skin, intestine and liver except having lower toxicity in spawning season (May and June) than non-spawning seasons. 92 specimens (76.7%) out of 120 ones were non toxic (below 10 MU/g) for the edible and 28 ones (23.3%) were weakly toxic (10 MU/g∼l00 MU/g) for the non-edible in muscle ranged between 0 MU/g and 46.4 MU/g. All of those toxicity were shown over 10 MU/g in skin for the non-edible in skin ranged from 10.0 MU/g to 674.4 MU/g. Toxicity range in intestine and liver was varied between non-toxic and strongly toxic (over 1,000 MU/g) for individual specimens. Toxicity range of the ovary found only in May (7 specimens) and June (1 specimens) was 84.2 MU/g to 2,191.3 MU/g.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.16 no.1
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• pp.27-35
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• 1980

The purpose of the present study is to find the light intensity which induces maximum gathering rate and to observe the variation of the gathering rate both in' daytime and at night by using two species of commercial fishes: rock bream, Oplegnathus fasciatus(Temminet et Schlegel) and glass-puffer,\ulcorner Fugu.niphobles (Jordan \ulcorneret. Snyder). An experimental tank($360L{\times}50W{\times}55H cm$) was set up in a dark room. An illumination system was attached to one end of the tank to control horizontal light intensity. Six artificial light sources were prepared by combination of two light bulbs (5W, 150W) and seven filters. During the. experiment water depth was maintained 50 em level in the tank. The tank was marked into six longitudinal sections each, being 60 cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 40 minutes prior to the main experiment. Upon turning on the light, the number of fish in each section was counted40 times every 30 seconds, and the gathering rates were obtain from th~ average number of. fish' in each section. The light intensity inducing maximum gathering rate is as follows: rock bream: 162.0 lux (104,3--238, 1 lux) (day), 162.0 lux (104.3--238.1 lux) (night). grass puffer: 16.6 lux (10.6--24.5 lux) (day), 1. 9 lux (1. 2-2. 9 lUX) (night). Trend .of the gathering rate in illumination time revealed different results in two fish species. Gathering rate of rock bream showed the increasing trend fluctuately with the lapse of ' illumination time. However, that of grass puffer showed the increasing trend gradually at the early period of the illumination time and the uniform trend at the latter period with little distictive fluctuation.