• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.255-262
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• 2020

The aim of this study was to investigate the correlation between growth characteristics and ginsenoside contents of 7 and 13-year-old wild-simulated ginseng. The results of growth characteristics such as rhizome length, root length, fresh weight, cross-section area, surface area and volume were shows significantly higher in 13-year-old wild-simulated ginseng compare to 7-year-old wild-simulated ginseng. In the case of 11 ginsenoside contents, the contents of G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 and Rg2 were shows significantly higher in 13-year-old wild-simulated ginseng compare to 7-year-old wild-simulated ginseng. In addition, in the comparative analysis of ginsenoside contents between wild-simulated ginseng and cultivated ginseng, 13-year-old wild-simulated ginseng was shows significantly higher G-Rb1, Rd, Re, Rf and Rg1 ginsenoside contents compare to 4-year-old and 5-year-old cultivated ginseng. In the result of correlation analysis between growth characteristics and ginsenoside contents, the G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2 ginsenoside was shows significantly positive correlation with rhizome length, fresh weight, cross-section area, surface area, volume, while as the contents of G-Rb1, Re, Rf, Rg2 was shows significantly negative correlation with shoot diameter. The results of this study was might be help to provide useful information on the establish quality standard by the investigate correlation analysis between growth characteristics and ginsenoside content of wild-simulated ginseng.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.403-410
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• 2021

The aim of this study was to investigate the growth characteristic and ginsenoside contents of 7 and 13 year-old wild-simulated ginseng (Panax ginseng C.A. Meyer) according to rusty root. The root growth characteristics of wild-simulated ginseng were did not shows significant difference according to the rusty root. The results of ginsenoside contents of wild-simulated ginseng according to rusty root, ginsenoside Rb1 and Rg1 of 7 year-old wild-simulated ginseng were had shows a significantly higher in rusty root compare to general root. On the other hand, ginsenodie Rc, Rd, Re and Rg2 were significantly higher in gerneral root. In the case of 13 year-old wild-simulated ginseng, the contents of ginsenoside did not shows to significant difference according to rusty root. The results of correlation analysis between growth characteristics and ginsenoside content of general root, the ginsenoside Rb2, Rc, Rd, Rf, Rg1 were positive correlation with root length, while as the ginsenoside Rd of rusty root was shows significantly negative correlation with root length. The results of this study was might be able to improve awareness of consumer related to rusty root of wild-simulated ginseng. Moreover, might be help to provide useful information on the establish quality standard and distribution system of wild-simulated ginseng.

• Korean Journal of Medicinal Crop Science
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• v.17 no.5
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• pp.346-351
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• 2009

To study the optimal cultivation for paddy soil, growth characteristics and ginsenoside content was investigated by both of poor drainage class (PDC) and imperfect drainage class (IDC) in three-year-old ginseng of varieties, Cheonpoong (CP), Yeonpoong (YP), Hwangsookjong (HS), and Jakyeongjong (JK). Root yield of IDC was higher than that of PDC by 3.6 times because stem length, leaf area, and chlorophyll contents were increased, while discolored leaf ratio was decreased. Root yield of HS in PDC was highest among four varieties because chlorophyll contents, leaf area, and survived plant ratio were relatively high. Root yield of CP in IDC was highest among four varieties because of high leaf area and survived plant ratio, and low discolored leaf ratio. Ratio of rusty-colored root showed significant difference by varieties, which was the highest in HS and the lowest in CP among four varieties irrespective of drainage classes. Total ginsenoside contents showed significant difference by drainage classes, which were high in IDC of good growth and low in PDC of poor growth. Total ginsenoside contents were high in JK and CP, while low in HS and YP both of drainage classes.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.52-56
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• 2005

This study was compared the quality of red ginseng and characteristic changes of physicochemical properties according to the storage period (non storage, two days, six days, eight days, ten days) and store temperature $20^{\circ}C, \;34^{\circ}C,\;-10^{\circ}C)$. The water content of the fresh ginseng has a tendency to decrease as storage time increases. When we store the fresh ginseng for 10 days, the ideal storage temperature is considered to be $34^{\circ}C$ degrees. The amount of total nitrogen has a tendency to increase more than that of no storage as storage period approaches to 10 days. In the storage temperature, the amount of total nitrogen has a tendency to increase in the order of 1) room temperature, 2) freezing storage, 3) cold storage more than no storage. Cold storage has larger contents of total phenolic compounds than room temperature and freezing storage according to storage temperature. When we analyze the changes of a relative density of eight elements, ginsenoside $Rb_1,Rb_2,Rc,Rd,Re,Rg_3,Rg_1\;and\;Rg_2$ in red ginseng's saponin Rf according to storage condition, the relative density of $Rb_1\;and\;Rg_1$ against Rf diminishes in each storage condition as storage time increases. And it is also thought that density change of ginsenoside appears because of the materials, and change tendency according to storage condition is not clear. From functional nature on the evaluation of the quality, taste and fragrance of red ginseng according to storage district, it is evaluated that it is most recommendable for red ginseng to be transported and stored in $3{\sim}4$ degrees to keep its best condition.

• Journal of Ginseng Research
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• v.38 no.3
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• pp.180-186
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• 2014

Background: This study evaluated changes in ginsenoside compositions and antioxidant activities in hydroponic-cultured ginseng roots (HGR) and leaves (HGL) with heating temperature. Methods: Heat treatment was performed at temperatures of $90^{\circ}C$, $110^{\circ}C$, $130^{\circ}C$, and $150^{\circ}C$ for 2 hours Results: The ginsenoside content varied significantly with heating temperature. The levels of ginsenosides Rg1 and Re in HGR decreased with increasing heating temperature. Ginsenosides F2, F4, Rk3, Rh4, Rg3 (S form), Rg3 (R form), Rk1, and Rg5, which were absent in the raw ginseng, were formed after heat treatment. The levels of ginsenosides Rg1, Re, Rf, and Rb1 in HGL decreased with increasing heating temperature. Conversely, ginsenosides Rk3, Rh4, Rg3 (R form), Rk1, and Rg5 increased with increasing heating temperature. In addition, ginsenoside contents of heated HGL were slightly higher than those of HGR. The highest extraction yield was 14.39% at $130^{\circ}C$, whereas the lowest value was 10.30% at $150^{\circ}C$ After heating, polyphenol contents of HGR and HGL increased from 0.43 mg gallic acid equivalent/g (mg GAE eq/g) and 0.74 mg GAE eq/g to 6.16 mg GAE eq/g and 2.86 mg GAE eq/g, respectively. Conclusion: Antioxidant activities of HGR and HGL, measured by 1,1-diphenyl-2-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging ability, increased with increasing heating temperature. These results may aid in improving the biological activity and quality of ginseng subjected to heat treatments.

• Journal of Ginseng Research
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• v.39 no.4
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• pp.314-321
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• 2015

Background: Ginseng belongs to the genus Panax. Its main active ingredients are the ginsenosides. Interstitial cells of Cajal (ICCs) are the pacemaker cells of the gastrointestinal (GI) tract. To understand the effects of ginsenoside Re (GRe) on GI motility, the authors investigated its effects on the pacemaker activity of ICCs of the murine small intestine. Methods: Interstitial cells of Cajal were dissociated from mouse small intestines by enzymatic digestion. The whole-cell patch clamp configuration was used to record pacemaker potentials in cultured ICCs. Changes in cyclic guanosine monophosphate (cGMP) content induced by GRe were investigated. Results: Ginsenoside Re ($20-40{\mu}M$) decreased the amplitude and frequency of ICC pacemaker activity in a concentration-dependent manner. This action was blocked by guanosine 50-[${\beta}-thio$]diphosphate [a guanosine-5'-triphosphate (GTP)-binding protein inhibitor] and by glibenclamide [an adenosine triphosphate (ATP)-sensitive $K^{+}$ channel blocker]. To study the GRe-induced signaling pathway in ICCs, the effects of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (a guanylate cyclase inhibitor) and RP-8-CPT-cGMPS (a protein kinase G inhibitor) were examined. Both inhibitors blocked the inhibitory effect of GRe on ICC pacemaker activity. L-NG-nitroarginine methyl ester ($100{\mu}M$), which is a nonselective nitric oxide synthase (NOS) inhibitor, blocked the effects of GRe on ICC pacemaker activity and GRe-stimulated cGMP production in ICCs. Conclusion: In cultured murine ICCs, GRe inhibits the pacemaker activity of ICCs via the ATP-sensitive potassium ($K^{+}$) channel and the cGMP/NO-dependent pathway. Ginsenoside Re may be a basis for developing novel spasmolytic agents to prevent or alleviate GI motility dysfunction.

• Journal of Ginseng Research
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• v.16 no.3
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• pp.190-197
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• 1992

The increased level of glucose, ketone bodies, non-esterified fatty acids and lactate in blood, decrease of glycogen content, phosphofructokinase activities and glucokinase activity and the increased level of glucose-6-phosphatase activity in the liver of streptozotocin injected rats were significantly moderated by ginseng saponin administration. It is not likely, however, that the hypoglycomic action of ginseng saponin might be due to their direct action on enzyme activities, since the saponin effect of servers enzymes in vitro was not enough to explain such an appreciable hypoglycemic activity of the saponin in streptozotocin induced diabetic rats, for which much work have to be done.

• Korean Journal of Pharmacognosy
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• v.47 no.4
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• pp.352-359
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• 2016

The purpose of this study is to develop a new preparation process of ginseng leaf and stem extracts having high concentrations of ginsenoside Rg2, Rg3, Rg5, Rh1, a special component of red and black ginseng. Chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by the HPLC. Extracts of ginseng (Panax ginseng) leaf and stem were processed under several treatment conditions including ultrasonication treatments. The content of total saponin reached their heights at 17 hr (UGL-17) of ultrasonication treatment, followed by 16 hr (UGL-16) and 7 hr (UGL-7) of ultrasonication treatment at $100^{\circ}C$. UGL-17 findings show that the ginseng leaf and stem that had been processed with ultrasonication for 17 hours peaked in the level of Rg2, Rg3 and Rh1. In addition, UGL-16 contained ginsenoside Rg5 at high concentrations. It is thought that such results provide basic information in preparing ginseng leaf and stem extracts with functionality enhanced.

• The Korean Journal of Food And Nutrition
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• v.11 no.1
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• pp.82-87
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• 1998

To study of production of Panax ginseng leaf tea, after harvested the leaves in July, August, and September as ripening season, the content and composition of ginseng saponin were investigated. 1. Crude saponin contents in the leaves were a about 16.5%, and they were found to be lower in the leaf harvested in September than those harvested in July or August. 2. As similar patterns were observed with month to month in ginsenoside, sum of major ginsenosides of -Re, -Rd and -Rg1 was fixed about 70% of saponin at harvested in each month. And minor components were ginsenoside -Rb1, -Rb2 and -Rc as in order. 3. The ratio of protopanaxadiol(PD)/protopanaxatriol(PT) was revealed reduction of 1.13 of harvested in July to 0.85 of those in September gradually. The contents of protopanaxadiol were high in the leaves of August and protopanaxatriol was high in those September.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1668-1674
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• 2016

Red ginseng, a steamed and sun-dried ginseng, is a popular health-promoting food in Korea and other Asian countries. We introduced nanofertilizer technology using gold nanoparticles in an effort to develop red ginseng with an elevated level of ginsenosides, the main active compounds of ginseng. Shoots of 6-year-old ginseng plants were fertilized three times with colloidal gold nanoparticle sprays. Red ginseng extract was prepared from the main roots. The concentrations of gold and ginsenosides were measured following gold nanoparticle treatment. To evaluate the anti-inflammatory effects, mouse peritoneal macrophages of male BALB/c mouse were stimulated with lipopolysaccharide plus interferon-γ in the presence of extracts from red ginseng with or without gold nanoparticle treatment. The content of ginsenosides, such as Rg1, Re, Rf, and Rb1, increased in ginseng treated with gold nanofertilizer whereas the steaming process increased only the levels of Rd and Rg3. The levels of nitric oxide, inducible nitric oxide synthase, and interleukin-6, but not tumor necrosis factor-α, were more suppressed in macrophages treated with extract from gold nanoparticle-treated red ginseng. Our results show that the use of a colloidal gold nanoparticle fertilizer improved the synthesis of ginsenosides in ginseng and enhanced the anti-inflammatory effects of red ginseng. Further research is required to elucidate the causal factors for the gold-induced change in ginsenoside synthesis and to determine the in vivo effect of gold nanoparticle-treated ginseng.