• Journal of Ginseng Research
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• v.41 no.3
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• pp.419-427
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• 2017

Background: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown. Methods: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay. In planta transformation in heterologous Arabidopsis was generated by floral dipping using Agrobacterium tumefaciens (C58C1). Protein localization was analyzed by confocal microscopy via fluorescent protein tagging. Results: PgUGT72AL1 was highly expressed in the rhizome, upper root, and youngest leaf compared with the other organs. GUS staining of the promoter: GUS fusion revealed high expression in different organs, including axillary leaf branch. Overexpression of PgUGT72AL1 resulted in a fused organ in the axillary leaf branch. Conclusion: PgUGT72AL1, which is phylogenetically close to PgUGT71A27, is involved in the production of ginsenoside compound K. Considering that compound K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of ginsenosides in ginseng plant growth and development. The organ fusion phenotype could be caused by the defective growth of cells in the boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, and requires further analysis.

• Korean Journal of Food Science and Technology
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• v.13 no.4
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• pp.267-272
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• 1981

The qualities of ginseng leaf teas prepared by six different processing methods were evaluated to develop the leaf tea. The leaf tea prepared by fermentation at $30^{\circ}C$ showed the highest in the ratio of 30 min water extracts to the total extractable matters. This ratio was followed in decreasing order by the heat dried tea and the one fermented at $25^{\circ}C$. The yellow, orange and red color intensities of water extracts were the highest in the tea prepared by toasting method followed by teas fermented at $30^{\circ}C\:and\:25^{\circ}C$. The amount of saponins extracted with boiling water was the highest in the tea fermented at $30^{\circ}C$ among teas investigated. This tea was also most acceptable in sensory evaluation by the flavour profile method.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.2
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• pp.138-142
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• 2000

The effects of stalk cutting time and environmental factors such as air temperature, leaf temperature, solar radiation and leaf moisture content during harvesting and curing in burley tobacco(Nicotiana tabacum L.) on weight loss of fresh stalks and sunburning in leaves were investigated at Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1996 and 1997. Twelve to fifteen percent of the fresh weight was lost in 3 to 4 hours after stalk-cutting, and sunburned leaves could be observed in case of stalk cutting between 11:00 and 15:00 O'clock on a clear sunny day, when the air temperature was 34 to 35$^{\circ}C$, leaf temperature 52 to 54$^{\circ}C$, and solar radiation 700 to 940 w/$m^2$. The leaves exposed to this weather condition were sunburned within 1 hour after stalk cutting. But low temperature (below $25^{\circ}C$) with high solar radiation(above 700w/m2) or high temperature(above 3$0^{\circ}C$) with low solar radiation (below 600w$m^2$) did not induce the sunburn damage in leaves. As the leaf temperature and leaf moisture content were higher, the sunburned leaves increased. The leaves at the higher stalk position were more easily sunburned than those at the lower. This result indicates that the immature leaves with higher chlorophyll content might be more susceptible to sunburning.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.158-164
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• 1989

In order to determine the relationships between the lea(-burning disease and the light harvesting chlorophyll-protein (LHCP) complex in Panax ginseng C. A. Meyer, we investigated the chlorophyll-protein (CP) complex of the thylakoid membrane and its characteristics. In P. ginseng four Cp-complex bands determined by non-denaturing SDS-PAGE were identified CP I'(containing reaction center of photosystem I and LHCP I antennae), CP I (reaction center of photosystem I) LHCP II** (oligoform of LHCP II), and LHCP II (photosystem II antennae, CP 26 and CP 29) by Bassis and Dunahay's procedures. Under our experimental condition, the CP I band was only observed in P. ginseng and the band intensity of LHCP II** in P ginseng was higher than in spinach and soybean. There were differences in the absorption and fluorescence spectra and chlorophyll a/b ratio of the CP-complex bands between P. ginseng and other Plants. The Polypeptidr content of P. ginseng thylakoid was lower than in spinach and soybean thylakoid, and the Polypeptide profiles of P. ginseng was low band intensity, especially about 29-35 kD, 55 kD, and 60 kD, compared to spinach and soybean. The inhibitory effects of 2,5-dimethylfuran, specific singlet oxygen ($^1O_2$) quencher, showed that singlet oxygen destroyed 60% of chl.a, 90% of chl.b and 70% of carotenoid in bleaching P. ginseng with leaf-burning disease.

• Journal of the Korean Society of Tobacco Science
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• v.10 no.1
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• pp.11-19
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• 1988

This study was conducted to relate among growth characteristics, quality, and chemical components for flue-cored tobacco. The results are summarized as follows : 1. Starch content in harvested green leaf was correlated negatively with leaf length, leaf width, leaf area, and leaf weight of harvested green leaf, respectively, while positively with total sugar content in cured leaf, and the ratio of leaf length to leaf width of harvested green leaf. 2. Organic matter and total nitrogen contents in the soil were correlated positively with nicotine content in cured leaf, respectively, and total nitrogen in the soil negatively with total sugar content in cured leaf. 3, Amount of fertilizer, application date of MH, priod of harvesting, and yield were correlated positively with nicotine content in cured leaf, respectively , while negatively with total sugar content. Application amount of MH was correlated positively with total sugar content, but negatively with nicotine content in cured leaf. Also amount of compost was correlated negatively with nicotine content in cured leaf. 4. The ratio of total sugar to nicotine (TS/N) per plant was correlated positively with price (Won/kg) and specific leaf area, but negatively with leaf length. leaf width, leaf area, harvested leaves, and leaf weight in cured leaves, respectively. Yield was correlated negatively with TS/N per plant. 5. TS/N of the best quality tobacco per plant was 12.0. Those of the best quality tobacco in each stalk position were 42.1 for first~third leaf, 28.4 for 4th~6th leaf , 23.7 for 7th~9th leaf, 7.7 for l0th~12th leaf , and 7.8 for over 13th leaf from bottom, respectively. 6, When TS/N was 12.0, optimum values of growth characteristics per plant were 100.5 $\pm$ 10.3g for leaf dry weight, 755.1$\pm$53.2cm for leaf length, 294.4$\pm$25.1cm for leaf width, 8, 892$\pm$111cm2 for leaf area, 16.0$\pm$0.6 leaves for harvested leaves, and 7.32$\pm$0.44mg/cm2 for specific leaf area, respectively. 7. When TS/N was 12.0, optimum values of chemical components per plant were 1.92$\pm$0.28% for nicotine content, and 20.4$\pm$ 1.4 % for total sugar content, and that of yield was 238.3$\pm$ 9.8kg/10a.

• Journal of Ginseng Research
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• v.17 no.3
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• pp.232-235
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• 1993

We investigated the effect of antioxidants (ascorbate, glutathione, and sodium azide), which efEectively inhibited the chlorophyll bleaching of Panax ginseng CA Meyer under the high light intensity, treated by folilar wiping on the early stage of photosynthesis and transpiration of ginseng in the 5000 $\mu$mol photon.$m^{-2}$.$s^{-1}$. Ascorbate and glutathione, endogenous antioxidant, completely recovered ginseng from the photoinhibition, but sodium azide, synthetic quencher, showed negative effect. We assumed that endogenous antioxidants could be available to the protection of the leaf-burning phenomenon of ginseng.

• Journal of Ginseng Research
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• v.7 no.2
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• pp.108-114
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• 1983

1. To know the site of saponin synthesis in this plant, 4-years old Panax ginseng C.A. Meyer was administered with 1, 2-l4C-acetate (Na salt, 10 ucilplant) by stem injection and was continued to grow for 3 weeks and the distribution of the radioactivity in leaf, stem and root part was identified. The percentage of radioactivity recovered was about 3.99%. 2. The sliced roots or leaf discs (2g) were bathed in the reaction mixture containing sugar, ATP, NADPH, and the distribution of the radioactivity of the fractions (sugar, saponin, sapogenin) was identified. 3. It seemed that major synthesized saponins in roots and leaves are dial and triol-type, respectively. Although both types of saponins are synthesized in roots, the main saponins seemed to be dial saponins and a significant portion of triol saponins are supplied from leaves through stem.

• Journal of Pharmaceutical Investigation
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• v.9 no.1
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• pp.1-6
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• 1979

This report is carried out pharmaceutical properties for the mosquito coil made of Ginseng leaf and the residue after extraction of Ginseng root. This experimental results are: Loss on drying of Insam (Ginseng) mosquito coil powder is average 8.31%, ash content of the powder is average 9.51% and burning time of Insam mosquito coil is aprroximately 36 minutes per gram. The contents of carbon monoxide and carbon dioxide on burning of mosquito coil are as same as following: Insam mosquito coil A brand mosquito coil B brand mosquito coil Allethrin in a piece of Insam mosquito coil was stable under the experimental conditions. CO (ppm) 360 540 760 $CO_2(%)$ 0.052 0.076 0.066 Allethrin in a piece of Insam mosquito coil was stable under the experimental conditions.

• Journal of the Korean Society of Tobacco Science
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• v.11 no.2
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• pp.247-253
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• 1989

The amino acid content and constitution of domestic flue-cured tobacco before and after the aging were investigated. The major amino acids of domestic flue -cured tobacco were histidine, serine, aspartic acid and proline and leucine, isoleucine and glycine were minority in them. Phenylalanine was the most changeable components through the aging. While the contents of tryptophan, sering and Proline which have herbaceous, harsh and bitter taste and aroma in smoke were reduced, tyrosine which adds smoothing and enhances smoke aroma was increased after the aging. The leaf tobacco aged at 4$0^{\circ}C$ for 30 days had a amino acid composition as good leaf tobacco storaged in natural condition for 2 years.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.5
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• pp.717-722
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• 2016

The purpose of this study was to evaluate the quality of madeleine added with different concentrations (0, 1, 3, 5, and 7%) of organic ginseng leaves. The study results found that the pH and moisture of madeleine with control were higher than those of the samples. On the other hand, specific gravity of madeleine was highest at 7% leaf content (1.04). The loss rate of madeleine was not significantly different among the samples. The Hunter L, a, and b values of crust decreased as the concentration of organic ginseng leaves increased. The Hunter L and a values of crumb decreased as the concentration of organic ginseng leaves increased, whereas b values of crumb increased. The hardness of madeleine increased after addition of organic ginseng leaves, whereas adhesiveness, chewiness, gumminess, and cohesiveness of madeleine decreased. 2,2'-Diphenyl-1-picrylhydrazyl radical scavenging activity of madeleine was significantly elevated with increasing content of organic ginseng leaves (P<0.05). In a sensory evaluation, healthy image and color were highest at 3% leaf content, whereas moistness, softness, and chewiness decreased as the concentration of organic ginseng leaves increased. The flavor and overall acceptability of madeleine added with 3% organic ginseng leaves were higher than those of both control and other samples. Therefore, the results suggest that 3% organic ginseng leaves addition to madeleine could be helpful for improving physical quality and taste.