• 한국약용작물학회지
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• 제3권2호
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• pp.77-80
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• 1995

한국산 광릉쥐오줌풀에 대한 광합성 및 호흡특성과 아울러 광합성의 일중(日中)변화와 이와 관련된 특성 및 생육상황에 미치는 온도의 영향을 조사하였던 그 결과는 다음과 같다. 1. 온도와 쥐오줌풀 잎의 광합성간에는 고도로 유의한 2차곡선회귀가 인정되었으며, 이 회귀식에 의해 산출한 최대광합성을 위한 은도는 $17.7^{\circ}C$이었다. 2. 쥐오줌풀 잎의 기공수는 잎의 표면에서 약 $25/mm^2$, 이면에서 $85mm^2$이었으며 기공의 크기는 $21{\sim}30/{\mu}m$로 나타났다. 3. 온도와 쥐오줌풀의 엽폭 및 근중간에는 각각 고도로 유의한 2차곡선회귀가 인정되었으며 이 회귀식에 의해 산출한 근생장(根生長)의 최적온도는 약 $20.3^{\circ}C$이었다.

• 미생물학회지
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• 제43권4호
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• pp.311-316
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• 2007

탄소원으로 thiodiglycol(TDG)을 함유한 배지에서 농후배양하여 인삼토양으로부터 TDG 분해균을 분리하였다. 분리균 WS-32의 형태적, 생화학적, 유전학적 특성을 조사한 결과 분리균이 Alcaligenes faecalis와 유사한 생화학적 성질을 지니고 있으며, 16S rRNA 서열이 Cupriavidus 속 균주와 유사도가 높은 균주로 판명되었다. WS-32는 $33^{\circ}C{\sim}37^{\circ}C$, pH $6.0{\sim}8.0$에서 성장이 우수하였으며, TDG에 의해 성장에 약간의 저해를 받지만, 배양후기에서는 이를 탄소원으로 이용하는 현상을 보였다. HPLC를 통해 배양액 내 잔존하는 TDG를 분석한 결과 2일 배양하였을 때 배지내 잔존하는 TDG가 상당량 감소한 것으로 확인되었다. 분리균 WD-32의 균체 파쇄상등액은 TDG의 산화활성을 보였으며, pH 8.0과 $45^{\circ}C$에서 산화활성이 가장 높았다.

• 사상체질의학회지
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• 제23권1호
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• pp.79-86
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• 2011

1. Objectives: Pharmacopuncture according to Sasang Constitutional Medicine is new field of constitutional medicine. We investigated safety of pharmacopunctures to develop this treatment. 2. Methods: We searched for papers in fifteen Korean oriental medical journals and four online searching portal articles. Hepatotoxicity, nephrotoxicity and general toxicity tests were investigated about one hundred thirty kinds of pharmacopunctures. 3. Results: Toxicity test about 36 kinds of 130 pharmacopunctures were investigated. 1) Hepatotoxicity and nephrotoxicity test of pharmacopunctures were searched in 11 of 38 Soeumin herbs, 8 of 36 Soyangin herbs, 5 of 44 Taeeumin herbs and 1 of 12 Taeyangin herbs. From among these herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Fructus alpiniae oxyphyllae(益智仁), Semen crotonis(巴豆), Radix bupleuri(柴胡) and Semen armeniacae amarum(杏仁) pharmacopunctures. 2) General toxicity test of pharmacopunctures were searched in 8 of 38 Soeumin herbs, 9 of 36 Soyangin herbs, 6 of 44 Taeeumin herbs and 2 of 12 Taeyangin herbs. from among thes herbs, toxicity was detected in Radix aconiti lateralis preparata(附子), Radix ginseng(人蔘), Semen crotonis(巴豆), Rhizoma seu radix notopterygii(羌活), Radix aralia cordatae(獨活), Semen armeniacae amarum(杏仁) and Radix scutellariae(黃芩) and Cortex acanthopanacis(五加皮) pharmacopunctures. 4. Conclusions: From these results, we must decide extraction method, concentration and dose carefully to use the toxic pharmacopuncture. More toxicity test and further study is needed about pharmacopuncture treatment in Sasang Constitutional Medicine.

• Journal of Species Research
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• 제7권1호
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• pp.13-23
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• 2018

In 2016, as a part of the research program 'Survey of Korean Indigenous Species', diverse environmental samples were collected from various sources of freshwater, seawater, soil, wetland, reclaimed land, sand, pine forest, plant root, ginseng field, solar saltern, and caves. Thousands of bacterial strains were isolated from the diverse samples and identified based on 16S rRNA gene sequence analyses. The present study, as a phylogenetic subset of the primary research program, reports 24 unrecorded bacterial species in Korea that belong to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria. Based on the high 16S rRNA gene sequence similarities (>98.8%) and formation of a robust phylogenetic clade with the closest type species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 24 bacterial species have been described in Korea; therefore, 10 species of nine genera in the order Rhizobiales and 14 species of seven genera in the order Sphingomonadales are described for unreported alphaproteobacterial species in Korea. Gram reaction, colony and cell morphology, biochemical properties, and isolation sources are also provided in the species description section.

• The Plant Pathology Journal
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• 제18권3호
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• pp.130-137
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• 2002

Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

• 식물조직배양학회지
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• 제26권3호
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• pp.177-182
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• 1999

Chitinase 유전자가 삽입된 감자 Belchip 품종의 형질전환체와 대조구에 곰팡이 병원균을 접종하였다. 7개 계통의 형질전환식물체를 12cm정도 키운 후 병원균인 Phytophthora infestans의 zoospore를 접종하여 인공적으로 역병을 유발시켰다. 그 결과 발병율에 따라서 세 그룹으로 분리되었는데 대조구에 비하여 감염 정도가 심한 것 2개 계통. 비슷한 3개 계통, 발병 정도가 약한 2개 계통으로 구분되었다. 대조구에 비하여 저항성이 높았던 2개 계통과 발병이 심했던 1개 계통만을 대상으로 하여 2차실험을 실시한 결과, 1차실험과 비슷한 경향을 나타냈다. 포장에서 생육된 2개의 저항성 계통은 자연적으로 발생한 역병에 대해서도 역시 대조구에 비하여 역병저항성이 더 높았다.

• 한국산업보건학회지
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• 제29권4호
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• pp.500-507
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• 2019

Objectives: The purpose of this study is to categorize farm types and analyze the cultivated area and labor input time for each crop over a year in order to decide farm type conducting for the hazard exposure assessment. Methods: Crops ranked within the top 45 by cultivated area in Korea were selected and 15 target crops that exposure assessment had been conducted for through the Rural Development Administration(RDA) pilot research projects were selected. Labor input time was based on data investigated through RDA research surveillance. If there was no data, data from Statistics Korea was used. Data from Statistics Korea was used for total cultivated area. Results: Crops were divided into 5 types: open field crops(Chinese cabbage, Corn, Ginger, Potato), fruits (Mandarin, Pear), green house crops(Cucumber, Lily, Oriental melon, Pumpkin, Tomato), livestock(Poultry), specialty crops(Ginseng, Oyster mushroom, Tobacco). The cultivated area for potato was the largest, and lily was the smallest among the crops. Labor input time over a year was the highest with oyster mushroom and the lowest with pear. Total labor input time by crops was the highest with mandarin and the lowest with Chinese cabbage. Conclusions: To evaluate hazard factors of farm work, it's essential that crops and tasks be classified based on the size of farmers and working hours by farm type. Therefore, the priority of management should be determined based on the cultivated area and total labor time, but the characteristics of tasks in each crop should also be considered.

• 동아시아식생활학회지
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• 제25권4호
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• pp.549-564
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• 2015

This study was conducted on the head family house of Panseogong coterie of Andong Kwon clans in Daksil village, Bongwha gun, Kyungbuk: historical sources survey and field visit over four rounds were conducted from March to June 2014. In-depth interview, storytelling, and nutrition facts were used as methods of research using 49 participants, and the potentials of trend goods were the main purpose of this research. The specialties of bongwha prefecture in the Chosun Dynasty were pine nut, manna lichen, ginseng, sweetfish, and wild honey. Ritual food bulcheonwi's offerings a feature in Choongjae Kwonbeol's head family - were sacred wine, me, gang, otang, myun, myunjeok, pyunjeok, pyunchung, pyun, po, chungpomook, dojeok, sookchae, ssam, chimchai, sikhye, chogwa, silgwa, soojunggwa, etc. The ritual foods of bulcheonwi's head family house Choongjae Kwonbeol were commercialized through storytelling. Choongjae Kwonbeol's ritual ceremonies, recognized for their national royalty, were reorganized as the traditional Korean table settings, so that the Korean people could easily access them. The special meal called "Geumgyeporansang" represented the head family house foods' essence to share and serve others through the Dacshil village's natural beauty, Bonghwa's local ingredients, and festivities after the "Chungjae Gwunbal Bulchun" rituals. The ritual foods called "Cheongamjungsang", utilizing "Chungjae Gwunbal Bulchun" rituals, were represented in the form of a lunch box: such foods illustrate the classical scholar's mindset that enjoys the nature and arts through education and virtue.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.114-115
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• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

• 대한한방소아과학회지
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• 제32권1호
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• pp.30-43
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• 2018

Objectives The purpose of this study is to analyze the clinical research literature of TCM (traditional Chinese medicine) which is applicable to pediatric fracture and to broaden the field about clinical application of Korean medicine treatment for pediatric fracture. Methods We searched randomized controlled trials about TCM treatment of pediatric fracture from the CNKI (China National Knowledge Infrastructure) January 2013 to December 2017. We searched the literature and analyzed the treatment methods and the results. Results 31 papers were selected from 63 studies. In most studies, the total effective rate of the treatment group was significantly higher than that of the control group, and the bone healing time of the treatment group was significantly lower than that of the control group. Methods of herb treatment include internal medicine (IM), fumigation (FG) and external application (EA). The most commonly used herb medicine were Angelicae Gigantis Radix (當歸), Carthami Flos (紅花), Drynaria Fortunei (骨碎補), Paeonia Lactiflora Pallas (芍藥), Persicae Semen (桃仁), Lycopodii Herba (伸筋草), Ligusticum Chuanxiong Hort (川芎), Olibanum (乳香), Salviae Miltiorrhizae Radix (丹蔘), and Panax Noto Ginseng (三七). Conclusions The use of herb medicine in pediatric fractures has resulted in faster and more effective restoration of fracture union than the Western medical therapy alone. Based on the results of this study, it is possible to widen the scope of Korean medicine if additional clinical studies on pediatric fracture were conducted.