• Title/Summary/Keyword: Ginseng field

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Mass Production of Adventitious Roots of Eleutherococcus sessiliflorus through the Bioreactor Culture

  • Seo Jin-Wook;Shin Cha-Gyun;Choi Yong-Eui
    • Journal of Plant Biotechnology
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    • v.5 no.3
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    • pp.187-191
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    • 2003
  • This paper reported the establishment of mass production system of adventitious roots of Eleutherococcus sessiliflorus through the shake flask and bio-reactor culture. Induction of adventitious roots was started from the explants of germinated somatic embryos on half-strength Murashing and Skoog (MS) solid medium. The frequency of adventitious root formation was better in the explants comprising the basal hypocotyl parts than root explants alone. Among the different auxins tested (NAA, IBA and IAA), frequency of adventitious root induction was highest on medium with 0.5 mg/L NAA, and produced $16.3\pm1.9$ roots per explant. In shake-flask culture, deletion of $NH_4NO_3$ of MS medium was effective for induction of adventitious root compared with both full and half-strength MS media. Fresh weight increase of induced adventitious roots was performed well in medium with 0.5 mg/L IBA. When adventitious roots produced in shake-flask culture were transferred to 10-liter bioreactor, 5.5 times of fresh weight increase was gained after one month of culture. HPLC analysis revealed that the amount of eleutheroside E and E1 was higher in in vitro cultured adventitious roots than the 3 year-old field cultivated root barks of Eleutherococcus sessiliflorus. The content of eltutheroside B was much lower in adventitious roots than that of field cultivated one.

UTILIZATION AND EVALUATION Of AVAILABLE PHOSPHORUS IN SOILS FOR FLUE-CURD TOBACCO (NICOTIANA TABACUM L.) II. FIELD EXPERIMENT (황색종 담배 (Nicotiana tabacum L.)의 토양중 유효인산의 이용과 그 평가 방법)

  • Park, Su-Jun;Choi, Jyung
    • Journal of the Korean Society of Tobacco Science
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    • v.6 no.2
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    • pp.155-168
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    • 1984
  • Field test were conducted to select the best method for soil phosphorus test and to obtain the critical levels of each method for P fertilizer recommendations based on tobacco production. Truog method was recognized to be the mort suitable one for soil P test. N. Carolina, Bray No.1 and Olsen methods were followed in order. Bray No. 2 and Lancaster methods showed the least satisfaction. The critical levels of available P by lancaster, Bray No. 1, N. Carolina,01sen, and Truog methods were 55ppm, 32ppm, 21ppm, 19ppm, and 22ppm, respectively. Below the critical level a large yield response can be expected with 10kg $P_2O_5$/10a, above critical level little or no response is expected. In soils the more the initial available p, the more available P was carried over, while the more the total amount of silt and clay, the less the residual P was found. The increase in residual P by 10kg of $P_2O_5$ was greater than that of 5kg/10a. The chemical composition and the price of the cured leaf were not affected by the $P_2O_5$ application, indicating that the increase in the rate of $P_2O_5$ application would not exert on the quality of tobacco.

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Disease Resistance of Race Differential Varieties and Improved Varieties(Nicotiana tabacum L.) on Black Shank (Phytophthora parasitica var. nicotianae) Infested Field (담배역병균(P. parasitica)에 오염된 포장에서의 Race 판별품종 및 육성품종(N. tabacum L.)의 발병반응)

  • 조천준
    • Journal of the Korean Society of Tobacco Science
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    • v.22 no.1
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    • pp.7-12
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    • 2000
  • This study was conducted to identify useful germ plasm sources of resistance to black shank, Phytophthora. parasitica, in Nicotiana tabacum and to characterize the interaction between host plant resistance to black shank and pathogen variability. The six internationally represen-tative set of black shank resistant and susceptible tobacco genotypes and four Korean lines were tested for black shank on the field infested with P. parasitica at Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute during the 1992~1997 growing season. Almost 100% of the plants of the most susceptible genotype, Ky 14, did not survive. Complete survival of (MS Ky 14 x L8)F$_1$, which is resistant to reee 0 and ausceptible to reace 1, suggests that Race 0 is the only race of the P. parasitica in this bield. Coker 371 Gold and Beinhart 1000-1 having excellent resistance to black shank could be recommended as the parental cultivars for improving black shank resistance in Korea. Data of the diseased plants showed that KB 101 and KB 110 were medium high resistant and KB 103 and KB 108 being low resistant to black shank.

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Varietal Difference of Leaf Breakdown in Field of Flue-Cured Tobacco (Nicotiana tabacum L.) (황색종 연초(Nicotiana tabacum L.)에서 엽탈락의 품종간 차이)

  • 조수헌
    • Journal of the Korean Society of Tobacco Science
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    • v.10 no.2
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    • pp.93-98
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    • 1988
  • This study was conducted to obtain basic information for varietal difference of leaf breakdown in field of flue-cured tobacco at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1987. The experiment was designed in randomized block with 3 replications, data ware analysed as Split split-plot design. Main plots were varieties, sub-plots were leaf positions, 4, 5 and 6th from bottom, and each sub-plot was divided into 3 parts with distance of midrib, 7 10 and 13em from stalk. Four varieties, NC 95, NC 2326, NC 82 and BY 4 were transplanted in 15 April, and measured the weight of leaf breakdown by artificially weighted in 5 June. The results obtained are as follows : 1. Weights of leaf breakdown according to leaf position, NC 95 were lower as 358-5799 than those of other varieties as 555-597g, were not significantly different regardless of varieties. 2. Weights of leaf breakdown in relation to distance of midrib from stack, NC 95 were lower as 309-419g than those of other varieties as 472-710g. 3. Weights of leaf breakdown were significantly different according to distance of midrib from stalk, and not significantly different according to leaf position under the same distance of midrib from stalk regardless of varieties.

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A Study on the Attributes Classification of Agricultural Land Based on Deep Learning Comparison of Accuracy between TIF Image and ECW Image (딥러닝 기반 농경지 속성분류를 위한 TIF 이미지와 ECW 이미지 간 정확도 비교 연구)

  • Kim, Ji Young;Wee, Seong Seung
    • Journal of The Korean Society of Agricultural Engineers
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    • v.65 no.6
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    • pp.15-22
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    • 2023
  • In this study, We conduct a comparative study of deep learning-based classification of agricultural field attributes using Tagged Image File (TIF) and Enhanced Compression Wavelet (ECW) images. The goal is to interpret and classify the attributes of agricultural fields by analyzing the differences between these two image formats. "FarmMap," initiated by the Ministry of Agriculture, Food and Rural Affairs in 2014, serves as the first digital map of agricultural land in South Korea. It comprises attributes such as paddy, field, orchard, agricultural facility and ginseng cultivation areas. For the purpose of comparing deep learning-based agricultural attribute classification, we consider the location and class information of objects, as well as the attribute information of FarmMap. We utilize the ResNet-50 instance segmentation model, which is suitable for this task, to conduct simulated experiments. The comparison of agricultural attribute classification between the two images is measured in terms of accuracy. The experimental results indicate that the accuracy of TIF images is 90.44%, while that of ECW images is 91.72%. The ECW image model demonstrates approximately 1.28% higher accuracy. However, statistical validation, specifically Wilcoxon rank-sum tests, did not reveal a significant difference in accuracy between the two images.

Variation of Microbial Community Along Depth in Paddy and Upland Field (논과 밭 토양에서 토층간 미생물 군집의 차이)

  • Kim, Chan-Yong;Park, Kee-Choon;Yi, Young-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.2
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    • pp.139-143
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    • 2009
  • We examined the vertical distribution of specific microbial groups and the patterns of microbial community structure within the soil profile using phospholipid fatty acid (PLFA). Samples were collected from the soil surface down to 15 cm in depth from paddy and upland fields located in Daegu, Korea. The two fields have been fertilized with only chemical fertilizers N, P, K for 33 years. Principal component analysis of the PLFA signatures indicated that the composition of the soil microbial communities changed significantly with the cultivation practices and soil depth, suggesting that cultivation practices of paddy and upland fields had more significant influence on soil microbial community than the soil depth did. The soil microbial communities changed more drastically with soil depth in upland field than in paddy field, with making thicker soil surface in paddy field in terms of soil microbial community. The ratios of cyclopropyl/monoenoic precursors and total saturated/total monounsaturated fatty acids increased with soil depth, suggesting that the deeper soil horizons are more carbon-limited and anaerobic than surface soil. The community analysis using PLFAs as biomarkers revealed that Gram-positive bacteria and actinomycetes tended to increase in proportional abundance with increasing soil depth, while the abundance of Gram-negative bacteria and fungi were highest at the soil surface and substantially lower in the subsurface.

Comparison of Growth Characteristics and Compounds of Ginseng Cultivated by Paddy and Upland Cultivation (논 . 밭재배에 따른 인삼의 생육 및 성분 특성 비교)

  • Lee, Sung-Woo;Kang, Seung-Won;Kim, Do-Yong;Seong, Nak-Sul;Park, Hee-Woon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.1
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    • pp.10-16
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    • 2004
  • This study was carried out to investigate the difference of growth characteristics and the content of root chemical components in four years old ginseng by paddy and upland cultivation at farmers' field in Korea. Proportions of silt, clay, liquid phase and porosity were higher in paddy soil than upland soil. The range of liquid phase was $17.5{\sim}19.5%$ in paddy and $7.0{\sim}12.8%$ in upland during growth period. EC and the other contents of OM, $NO_3^-,\;K_2O$, and Mg in paddy soil were higher than those of upland soil, while the contents of $P_2O_5$ and Ca were less than those of upland soil. The levels of chemical components of tested soil exceeded recommended range in EC, $NO_3^-$ and Ca of paddy soil, and in $P_2O_5$ and Ca of upland soil. Stem length, fresh root weight and total dry weight per plant in paddy were greater than those of upland. Root weight in paddy-ginseng showed a great increase on September, while it was not increased in upland because of early defoliation. Net assimilation rate and crop growth rate by paddy and upland cultivation showed distinct differences on May and September, and those of paddy-ginseng were higher than those of upland-ginseng. Yield and ratio of red-colored root showed no significant difference by paddy and upland cultivation, while significant differences were observed in diameter and length of primary root, contents of crude saponin and 50% ethanol extracts of primary root, and water content of root. Hardness of primary root showed no significant difference by paddy and upland cultivation until August, but it showed distinct difference on September, at which the hardness in upland cultivation was drastically decreased.

The Determination of Food-borne Microorganism using Chemiluminescence Method (화학발광 (Chemiluminescence)법을 이용한 식품 오염 미생물의 측정)

  • Kim, Yun-Mi;Lee, Hye-Ryun;Chung, Shin-Kyo
    • Current Research on Agriculture and Life Sciences
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    • v.24
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    • pp.9-16
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    • 2006
  • Ultraweak photons could be emitted from the chemical reaction without any electromagnetic radient. These chemiluminescence could be detected by highly sensible photomultiplier recently. We will introduce the principal of chemiluminescence generation reaction, and the application studies in the determination of food-borne microorganisms in field. The counts of surface contaminated microorganism in mackeral and chicken for cold storage determined by ATP-chemiluminescence method had good correlation with the counts by standard agar plate method. However, there was little correlation between 2 methods in ginseng powder whose microbial counts were lower than 10,000 CFU.

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