• KOREAN JOURNAL OF CROP SCIENCE
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• 제30권1호
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• pp.15-19
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• 1985

This study was carried out to develope the equations for estimating the areas of leaflet, leaf, and total leaf for 1, 2, 3, 4, 5, and 6 years old ginseng, Panax ginseng, grown in field. The highest correlation coefficient was found between leaflet area and product of leaflet length and width(LW) in all leaflets although leaflet shape varied somewhat according to the position and plant age. It was possible to estimate area of the leaf, and total leaf by one central leaflet in a compound leaf. The equations for estimating the leafet, leaf areas of 1 year differ to those of over 2 years old plant, but there was no difference among those of 2, 3, 4, 5, and 6 years. The equations for 1 year old are A =0.64 LW, A' =A/0.38, and for 2, 3, 4, 5, and 6 years old, A =0.60 LW, A' =A/0.32, A" =A' x number of leaves of central leaflet(A), leaf(A') and total leaf areas(A"), respectively. The estimation of leaflet, leaf, total leaf areas of ginseng plant grown under 20% light-transmittance rate was possible by using the equations mentioned.

• Journal of Environmental Science International
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• 제29권5호
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• pp.435-443
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• 2020

The purpose of this study was to investigate eco-friendly measures to manage major diseases which cause heavy economic damages to ginseng. Morphological, physicochemical, and molecular biological species identification was carried out after isolating useful antagonistic bacteria from ginseng fields. In addition, optimal conditions for mass culture were established, and he efficacy of the bacteria in the prevention of the diseases was verified in the field. The results showed that about 150 bacteria were extracted from 150 ginseng fields in the whole county. Among them, B. pyrrocinia LA101 was finally selected, which had a strong antagonistic potency against Alternaria panax, Botrytis cinerea, Rhizoctonia solani, and Cylindrocarpon destructans on agar media. The B. pyrrocinia LA101 is a baculiform gram-negative bacterium identified as Burkholderia pyrrocinia according to results from an API(Analytical Profile Index) kit, 16S rRNA, and gyrase gene sequencing analysis. It was donated to the microbe bank of the Agricultural Genetic Resources Center at the National Academy of Agriculture Science under the Rural Development Administration on September 28, 2011 (Donation No. KACC91663P). A patent for the mass culture technology was granted in August 2012 (Patent No. 10-1175532).

• Korean Journal of Soil Science and Fertilizer
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• 제27권4호
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• pp.315-320
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• 1994

This survey report introduced briefly peat deposits in SAKHALIN island of eastern SIBERIA continent. Peat industry was promoted as leading resources for development in this island by national control economic plan of central government before CIS. But development of deposit was left suspension of operation due to investment now. In southern area, about 10km from Yuzunosakhalinsk, two deposits of peat field were explored. One of them (Troisk) was suspended at begining stage of peat harvest from 300ha field and the other(Missilop) was producing the compact peat through drying and pulverizing from 350ha peat field which showed long run harvest activities. There are plenty of peat deposits near the Polonai Reika (river) in the central area of this island, At Zbaikaritz, 40km nothern area from Polonaisk, 135ha of peat field was developed. This field was left without working but established field preparation and could be able to be harvested any time if machines and utlities were settled. At southern suburb 10km from Polonaisk, about 40ha field of virgin deposit has been established planning for development. The plan involved estimated amount of peat, deposit depth, water level, drainage and leading road. The most of peat deposits accumulated from sphagnum moss. Texture is coarse but it contains more than 95% of organic carbon near to 0.5% of total nitrogen and acidity showed pH4-5.

• Journal of Food Hygiene and Safety
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• 제34권1호
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• pp.40-51
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• 2019

Ginseng has a unique production system that is different from those used for other crops. It is subject to the Ginseng Industry Act., requires a long-term cultivation period of 4-6 years, involves complicated cultivation characteristics whereby ginseng is not produced in a single location, and many ginseng farmers engage in mixed-farming. Therefore, to bring the production of Ginseng in line with GAP standards, it is necessary to better understand the on-site practices of Ginseng farmers according to established control points, and to provide a proper action plan for improving efficiency. Among ginseng farmers in Korea who applied for GAP certification, 77.6% obtained it, which is lower than the 94.1% of farmers who obtained certification for other products. 13.7% of the applicants were judged to be unsuitable during document review due to their use of unregistered pesticides and soil heavy metals. Another 8.7% of applicants failed to obtain certification due to inadequate management results. This is a considerably higher rate of failure than the 5.3% incompatibility of document inspection and 0.6% incompatibility of on-site inspection, which suggests that it is relatively more difficult to obtain GAP certification for ginseng farming than for other crops. Ginseng farmers were given an average of 2.65 points out of 10 essential control points and a total 72 control points, which was slightly lower than the 2.81 points obtained for other crops. In particular, ginseng farmers were given an average of 1.96 points in the evaluation of compliance with the safe use standards for pesticides, which was much lower than the average of 2.95 points for other crops. Therefore, it is necessary to train ginseng farmers to comply with the safe use of pesticides. In the other essential control points, the ginseng farmers were rated at an average of 2.33 points, lower than the 2.58 points given for other crops. Several other areas of compliance in which the ginseng farmers also rated low in comparison to other crops were found. These inclued record keeping over 1 year, record of pesticide use, pesticide storages, posts harvest storage management, hand washing before and after work, hygiene related to work clothing, training of workers safety and hygiene, and written plan of hazard management. Also, among the total 72 control points, there are 12 control points (10 required, 2 recommended) that do not apply to ginseng. Therefore, it is considered inappropriate to conduct an effective evaluation of the ginseng production process based on the existing certification standards. In conclusion, differentiated certification standards are needed to expand GAP certification for ginseng farmers, and it is also necessary to develop programs that can be implemented in a more systematic and field-oriented manner to provide the farmers with proper GAP management education.

• The Korean Journal of Pesticide Science
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• 제16권3호
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• pp.217-221
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• 2012

This study was carried out to evaluate residual characteristics of tolclofos-methyl in ginseng and elucidate the reason for its high detection rate from fresh ginseng selling at markets. Seeds of ginseng were sowed after seed dressing with tolclofos-methyl and after a year of growth, the young seedlings were transplanted to field. They were then harvested annually until three-years of growth and the pesticide residue was analyzed in them. LOD and LOQ of the pesticide were 0.001 and 0.003 mg/kg, respectively. Recovery test was carried out to validate the analytical method for tolclofos-methyl in ginseng. The ginseng seedlings were fortified with the test pesticide at the level of LOQ, ten times of LOQ and maximum residue concentration of tolclofos-methyl. Its recovery ranged from 77.37 to 100.16%. Residual concentration of tolclofos-methyl in ginseng seedlings just before transplanting and two-year-old ginseng were from 7.58 to 8.05 and from 6.46 to 6.79 mg/kg, respectively. In case of three-year-old ginseng, it was found to be from 4.18 to 4.35 mg/kg. As a result of annual pesticide residue analysis, concentration of the pesticide was found to decrease time-coursely in ginseng. This may be due to decomposition and increasing of fresh weight of the ginseng during the cultivation periods of three years.

• Horticultural Science & Technology
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• 제35권4호
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• pp.499-509
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• 2017

Recently, there has been increased attention to the development of new plant cultivars with enhanced resistance to biotic and abiotic stress. To develop ginseng cultivars with such traits, systematic breeding programs and comprehensive field studies are prerequisites. In this study, we applied a pure-line selection method to identify a ginseng cultivar with enhanced stress resistance. Phenotypic and agronomic characteristics, seed yield, and physiological responses to biotic and abiotic stresses were investigated according to the guidelines of the International Union for the Protection of New Varieties of Plants (UPOV). In the newly developed 'Kowon' cultivar, the time of emergence, flowering, and berry maturity were intermediate between those of the controls, 'Yunpoong' and 'Chunpoong'. The stem length of 'Kowon' was intermediate, whereas the root length was shorter and the main root diameter was greater than those of 'Chunpoong'. In local adaptability tests conducted in three regions, the yield of 'Kowon' was $666kg{\cdot}10a^{-1}$; 27% and 4% higher than that of 'Chunpoong' and 'Yunpoong'. Diseases such as Alternaria blight, Phytophthora blight, mulberry mealybug, and nematode infestation did not occur in 'Kowon'; and it also exhibited moderate resistance to damping-off and anthracnose. In these cases, yellow spots occurred on aerial parts and the rusty skin of the root, and it exhibited moderate resistance at high temperatures. Our study demonstrates that 'Kowon', which has a high root weight and enhanced biotic/abiotic stress resistance, is a superior cultivar that could increase farmers' income.

• Korean Journal of Medicinal Crop Science
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• 제19권2호
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• pp.90-96
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• 2011

This study was conducted to examine major growth responses, the production and partitioning of dry matter on different growth stages, and yields and to select the optimal shading material in both quality and productivity of ginseng. Two cultivars of ginseng, Cheonpoong and Geumpoong, were cultivated in the paddy soil with three different shading materials; three-layered blue and one-layered black polyethylene (TBP), blue polyethylene sheet (BPS), and aluminium-coated polyethylene sheet (APS). Plant heights were linearly increased until June 24 and then maintained with showing higher height in Cheonpoong than that in Geumpoong cultivar. Root lengths were gradually increased until October 16. They were longer in Cheonpoong than that in Geumpoong cultivar, showing slightly longer with APS compared to TBP and BPS. The ability of producing dry matter of leaves was much higher from April to June compared to those of other growth periods, whereas its ability of root was concentrated from the end of June to the end of August. Among the shading materials, the ability of producing dry matter of shoot was higher with TBP than those with BPS and APS, while its ability of root was not appeared certain tendency unlike the shoot. The yield of ginseng roots was the highest with TBP among three shading materials and it was higher in Cheonpoong than that of Geumpoong cultivar. The shading materials which affect the light intensity and the temperature would be considered as an important factor to get better quality and productivity of Korean ginseng.

• Korean journal of applied entomology
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• 제18권1호
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• pp.35-41
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• 1979

Four to $17\%$ of the seeds of ginseng (Panax ginseng Meyer) collected from seemingly healthy plants carried Colletotrichum panacicola Nakata et Takimoto whereas the seeds from the plants with anthracnose sympotoms carried $42\%$ of the same fungus. Prevalent organisms isolated other than C. panacicola from seeds of both kinds of plants were Fusarium, Alternaria, Phoma, Trichoderma and others, ana in that order on acidified potato sucrose agar. C. panacicola also was isolated from 18 months old herbarium specimens. The fungus in the infected tissues also survived during the Korean winter months either on the soil surface or in the soil at 10 and 30 em in depth. When conidial suspensions of C. panacicola were inoculated on detached ginseng leaves, anthracnose symptoms occurred from 25 to $35^{\circ}C$. No symptoms occurred at temperatures below $17^{\circ}C$. Direct sunlight increased significantly the number of anthracnose lesions over those obtained in leaves inoculated in darkness or in 400 lux of fluorescent light. The lesions decreased as age of the leaves increased or as the number of conidia applied decreased. Optimum temperature for mycelial growth and conidial formation of C. panacicola was $25^{\circ}C$. Optimum pH for the mycelial growth was at $pH\;2.8\~4.6$ while the most conidial formation occurred at $pH\;5.2\~5.8.$. When fungicides were applied in the field to ginseng plants with a conidial suspension of C. panacicola, the most effective control of the anthracnose disease was by spraying with difolatan, and followed by maneb, zineb, captan and phaltan; Bordeaux mixture and ferbam were significantly less effective but significantly better than the inoculated control plants.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.694-702
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• 1995

Using the Korean red ginseng saponin, which is known to world-wide and thd effects of it have been investigated by many reserachers for years. Ginseng saponin, one of the major components of Korea ginseng root, has many various biologic effects, such as cytotoxic effect, tumorcidal activity, protein biosynthesis and membrane modifying effect. The purpose of this study was to evaluate effects of ginseng saponin on the alkaline phosphatase activity of ROS cells in culture. After ROS cells were seeded into a 96-well plate, 96-well plate cultured until confluence was obtained. To evaluate cytotoxic effect of total saponin in cultured ROS cells, the plates were added to each total saponin concentration (0-1mg/ml). After 48hr., cells were counted by stain with 0.2% trypan blue at randomly selected field microscopically. Also, to evaluate alkaline phosphatase(ALP) activity of total saponin in cultured ROS cell, the plate was added to each total saponin concentration (0-1mg/ml) and ALP activity was assayed. To evaluate time-course of ALP activity, $31.25{\mu}g/ml$ of saponin added to 96-well plate. After culture of 6, 12, 24 and 48hr., ALP activity test was performed. To evaluate effect of cycloheximide in ALP activity, 96-well plate was added to saponin and cycloheximide. In control group, the plate was added saponin only. The results were as follows. 1. After the various concentration of total saponin was added in the medium, 500 and $1000{\mu}g/ml$ of total saponin showed cytotoxic effect of ROS(P<0.005). 2. In contrast to control group, 7.6, 15.6, 31.25, 62.5 and $250{\mu}g/ml$ of total saponin increased ALP activity significantly. 3. Otherwise, 500 and $1000{\mu}g/ml$ of total saponin decreased ALP activity significantly(P<0.005). 4. As the time span increases, $31.25{\mu}g/ml$ of total saponin increased ALP activity. 5. Cycloheximide decreased saponin-indueced ALP actitity in ROS(P<0.005). These results suggest that Ginseng total saponin stimulates the ALP activity of rat osteoblastic cells.

• Korean Journal of Plant Tissue Culture
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• 제27권6호
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• pp.485-489
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• 2000

The patterns and contents of ginsenosides were examined in normal root parts and hairy root lines of Panax ginseng C. A. Meyer. Ginsenoside-Rb$_1$, -Rb$_2$, -Rc, -Rd, -Re, -Rf, -Rg$_1$, -Rg$_2$ were detected in normal roots and hairy roots of ginseng. The patterns and contents of ginsenosides in that were very difference each other. The contents of total ginsenoside of hairy root (KGHR-1) was 17.42 mg/g dry wt, it's highest compared to others. Ginsenoside contents of hairy root (KGHR-1) was higher on ginsenoside-Rd, Rg$_1$, KGHR-5 was higher on ginsenoside-Rb$_1$, Rg$_1$, and KGHR-8 was higher on ginsenoside-Rd, Re than others. The contents of total ginsenosides on 6 years old ginseng cultured in the field were high in the order of main root, lateral root and fine roots, and content of ginsenosides in fine roots was 3.2 times higher than that in main root. The ratio of ginsenoside-Rg$_1$to total ginsenosides were about 3.43%, 8.68% and 14.18% respectively on fine root, lateral root and main root, it's very lower than that in hairy roots. It is suggested that specific ginsenosides can be produce in cultures of ginseng hairy roots.