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Bioactive Components and Volatile Compounds According to Illite Addition in Saururus chinensis Baill Cultivation (일라이트 시용량에 따른 삼백초 잎의 유효성분 및 향기성분 함량 비교)

  • Lee, Ka Soon;Kim, Gwan Hou;Seong, Bong Jae;Kim, Sun Ick;Han, Seung Ho;Lee, Sox Su;Lee, Min;Yoo, Chan Ho
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.3
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    • pp.188-195
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    • 2014
  • This study was carried to investigate the effect of illite addition on the growth, bioactive components and volatile compounds of leaf on Saururus chinensis Baill cultivation in greenhouse. Illite addition on Saururus chinensis Baill cultivation resulted no significant effects on the aerial part. However, the root part was highest in 5% illite addition group. Crude oil and ash amount increased as illite additives was increased. Ca of inorganics and free sugars contents were highest in 5% and 20% illite addition, respectively. Hyperoside and isoquercetin of available component on leaf were highest in 5% illite addition group, 4.02mg/g and 4.31mg/g, respectively. The volatile compounds in Saururus chinensis Baill leaf cultured with illite addition amounts were isolated by solid-phase microextraction fiber (polydimethysiloxane $65{\mu}m$) and identifed by gas chromatogtaphy mass spectrometry. As the results, the 22 volatile compounds were identified from in Saururus chinensis Baill leaf and major volatile compounds were the ${\alpha}$-cadinol (18.50%), myristicin (16.46%), methyl-9-methyl-tetradecanoate (10.22%), and ${\gamma}$-muurolene (9.75%). Especially, the content of ${\alpha}$-cadinol was highest in 5% illite addition group and ${\gamma}$-muurolene on overall illite addition group was lower than no addition group.

Nutritional Characteristics and Screening of Biological Activity of Cultured Wild Ginseng Roots (산삼 배양근의 영양성분 및 생리활성 탐색)

  • Park, Sung Jin;Yoo, Seon Mi;Kim, Young Eon
    • The Korean Journal of Food And Nutrition
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    • v.25 no.4
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    • pp.729-736
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    • 2012
  • The purpose of this study is to determine the possibility of using cultured wild ginseng roots as a natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of cultured wild ginseng roots were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 61.72%, 17.36%, 0.23% and 10.90%, respectively. Further, the calories of cultured wild ginseng roots were 323.97 kcal. Total dietary fiber was 82.13%. The protein contained a total of 18 different kinds of amino acids. The contents of amino acids were 16.15 g. The K was the largest mineral followed by P, Ca, and Mg, which means cultured wild ginseng roots is alkali material. The contents of saturated fatty acids and unsaturated fatty acids were 0.23 g, and 0.62 g, respectively. Crude saponine content was 25.87 mg/g. Total phenolic contents of cultured wild ginseng roots were 11.2mg/g, and total flavonoids contents were estimated as 4.2mg/g. The electron donating ability of cultured wild ginseng roots were 24.7~31.6%. The nitrite scavenging activity was pH dependent, and was highest at pH 1.2 and lowest at pH 6.0. The cultured wild ginseng roots extract showed the highest reducing power (0.06) at the concentration of $1,000{\mu}g/m{\ell}$. Based on the above results, we deemed that the cultured wild ginseng roots might have potential antioxdant activities.

Effect of Korean Red Ginseng on Blood Pressure and Aortic Vascular(endothelial) Histological Changes in Rats (홍삼이 정상 흰쥐의 혈압 및 대동맥 조직 소견 변화에 미치는 영향)

  • Joo, Il-Woo;Sung, Kyung-Hwa;Park, Jung-Min;Lew, Jae-Hwan;Oh, Han-Jin
    • Journal of Ginseng Research
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    • v.32 no.4
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    • pp.324-331
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    • 2008
  • Introduction : Korean red ginseng has been shown to have an preventive effect on atheroma formation and enhancing effect on nitric oxide synthesis in endothelial cell inducing vasodilatation. However, there are few studies showing the effect of Korean red ginseng on blood pressure and vascular(endothelial) pathologic changes together. We designed this study to show changes of blood pressure and vascular pathologic findings with Korean red ginseng administration compared with Chinese red ginseng and control for 3 months in rats. Materials and methods : We studied the in vitro hypotensive effect and effect on vascular pathologic changes of Korean red ginseng compared with Chinese red ginseng and control. Rats were randomly assigned to three groups(Korean red ginseng, Chinese red ginseng and control) and evaluated by blood pressure and aortic vascular(endothelial) pathologic changes monthly during 3-month administration. All results were analyzed by paired T-test, ANOVA and post-hoc. Results : Blood pressure lowering effect was noted on Korean red ginseng and Chinese red ginseng compared to control. Especially, in Korean red ginseng group, hypotensive effect was showed in first and second month, but, in Chinese red ginseng group, it was just noted in first month. In case of vascular(aortic endothelial) pathologic finding, endothelial wall thickening and elastic fiber tearing were noted in Chinese red ginseng group compared with Korean red ginseng group and control with statistical significance.(p<0.05) Discussion : These results suggested Korean red ginseng could have more hypotensive effect and maintenance rather than Chinese red ginseng. And the difference of hypotensive effect between Korean red ginseng and Chinese red ginseng might has some association with difference of vascular pathologic findings in each group. However, further evaluation and research of other mechanism will be needed to convince this hypothesis.

In vitro and in vivo evaluation of tissue-cultured mountain ginseng on penile erection

  • Lee, Ho Sung;Lee, Young Joo;Chung, Yoon Hee;Lee, Moo Yeol;Kim, Sung Tae;Ko, Sung Kwon;Momoi, Mariko;Kondoh, Yutaka;Sasaki, Fumio;Jeong, Ji Hoon
    • Journal of Ginseng Research
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    • v.40 no.4
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    • pp.334-343
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    • 2016
  • Background: Progressed tissue culture techniques have allowed us to easily obtain mass products of tissue-cultured mountain ginseng over 100 yr old (TCMG-100). We investigated the effects of TCMG-100 extract on erectile function using in vitro and in vivo studies. Methods: To examine the relaxation effects and mechanisms of action of TCMG-100 on rabbit cavernosal strips evaluated in an organ bath. To investigate the long-term treatment effect of TCMG-100, 8-wk administration was performed. After administration of TCMG-100, intracavernosal pressure, cyclic guanosine monophosphate and nitric oxide (NO) levels of cavernosal tissue, serum testosterone level, histological observation of collagen fiber, endothelium, smooth muscle cell, and transforming growth factor-${\beta}1$ were investigated. Results: TCMG-100 extract displayed dose-dependent relaxation effects on precontracted rabbit corporal smooth muscle. The TCMG-100-induced relaxation was significantly reduced by removing the endothelium, and treatment with an NO synthase inhibitor or NO scavenger. Eight weeks of TCMG-100 administration increased intracavernosal pressure in a rat model. The levels of cyclic guanosine monophosphate and NO in the corpus callosum and serum testosterone level were also increased by TCMG-100 treatment. Furthermore, histological evaluation of collagen, smooth muscle, and endothelium showed increases in endothelium and smooth muscle, and a decrease in transforming growth factor-${\beta}1$ expression. Conclusion: These relaxation effects on corporal smooth muscle and increased erectile function suggest that TCMG-100 might be used as an alternative herbal medicine to improve erectile function.

Comparison of the Chemical Components between Fresh and Odorless Garlic (생마늘과 무취마늘의 화학성분의 비교)

  • Lee, Mi-Kyung;Lee, Hyung-Ok;Lee, Seong-Kye;Do, Jae-Ho;Kim, Man-Wook;Lee, Jong-Won
    • Applied Biological Chemistry
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    • v.40 no.5
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    • pp.400-403
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    • 1997
  • The purpose of this study was to investigate the differences in proximate composition, free sugars, amino acids, fatty acids and Hunter color vaules between fresh and odorless garlic. The contents of crude fiber, crude fat and ash in odorless garlic were silmilar to those in fresh garlic. Sugars and crude protein decreased during manufacture of odorless garlic. Among the free sugars, sucrose, maltose and fructose decreased in odorless garlic, while the content of glucose increased. Total amino acid content decreased by about 30% in odorless garlic. Among the amino acids decreased arginine, isoleucine etc. The contents of linoleic acid, palmitic acid and oleic acid were higher than those of other fatty acids in fresh garlic and odorless garlic, but laruric acid was in little quantity. There were no singnificant differences in the Hunter L and b value between the fresh and odorless garlic, but value for greeness(-a) was lower in odorless garlic.

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A Study on the Nutritive Effect of Ginseng Meal in Laying Hen (산란계(産卵鷄)에 대(對)한 인삼박(人蔘粕)의 영양학적(營養學的) 효과(?果))

  • Ju, H.K.;Lee, K.U.;Choi, B.K.;Bak, M.Y.;Hong, S.P.
    • Korean Journal of Food Science and Technology
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    • v.7 no.1
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    • pp.11-14
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    • 1975
  • This work was carried out to determine that ginseng cake (meal) could be substituted for 10% level of chicken feed. The feeding experiment was held with 180 laying hens for 8 weeks. And the results were as tin the followings, 1. Protein content of the ginseng cake was lower (4.25%) than that of the control feed (18.72%: commertial chicken feed), and crude fiber of the ginseng cake was higher (14.73%) than that of the control (9.39%). 2. Slight increasings of body weight were observed in the group of the control feed (10 g) and the group of the feed containing 10% of the ginseng meal (24 g), significant increasing (50 g) was seen in the group of the feed containing 5% of the ginseng meal (p<0.05). 3. The feed efficiency of the ginseng group was slightly higher than that of the control group, but significant defferences were not recognized. 4. Egg laying ratio of the 5% ginseng group was somewhat higher than that of the other groups, and egg weight of each group was almost same. With all the results of this experiments, it was identified that ginseng cake is able to be substituted for the feed of laying hen within 10% level.

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Relationship between The Age and Chemical components of Ginseng Root's Portion(Panax ginseng C.A.Meyer) (인삼(人蔘)의 부위별(部位別) 및 연근별(年根別) 성분함량(成分含量)에 관(關)한 연구(硏究))

  • Lee, C.H.;Nam, K.Y.;Choi, K.J.
    • Korean Journal of Food Science and Technology
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    • v.10 no.2
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    • pp.263-268
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    • 1978
  • To find out the possible variation in chemical composition among ginseng products, the amount of saponin, extract and other basic components in different age and portion of ginseng roots(Panax ginseng Meyer) were investigated and compared with. (1) Great difference in the amount of ash, crude protein, fiber, fat, total sugar and reducing sugar was observed among different portion of the root comparing with those of different age of the root. That of ash, crude fiber, saponin and extract produced was higher in epidermis, fiber roots and subterranean stems, while that of crude protein, total sugar, panaxadiol/panaxatriol was higher in central portion and branch of the root. (2) The amount of extract produced was affected by the solvent used. Higher amount was obtained when water was employed. It was decreased as the increase of the concentration of alcohol solvent. Futhermore, the composition and physical properties were greatly varied by the concentration of alcohol solvent. (3) The amount of total-N, $P_2O_5,\;K_2O$, and ash was higher in two to three years old roots, while those of crude $SiO_2,\;CaO,$ crude fiber, and total sugar was higher in order roots. No difference was found in amount of MgO, Fe, Zn, and Na among age of the root.

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Physicochemical Characteristics of Various Ginseng Seeds (품종 별 인삼 씨의 이화학적 성분 비교)

  • Kim, Hee Jung;Yoo, Kyung Mi;Lee, Seul;Kim, Kyung-Tack;Hwang, In Kyeong
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.274-278
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    • 2013
  • The aim of our study was to investigate the chemical composition of the Asian ginseng seed (Panax ginseng C.A. Meyer) and the American ginseng seed (Panax quinquifolium L.) grown in Korea (3 years, KGS3; 4 years, KGS4), China (4 years, CGS4), and USA (4 years, AGS4). AGS had the heaviest 100-seed weight ($4.21{\pm}0.31g$). The approximate compositions of the ginseng seeds were 13.66-17.00% crude protein, 2.21-8.65% crude ash, 19.06-24.06% crude lipid, and 43.21-47.49% crude fiber. The mineral contents of the ginseng seeds were greater in order of K>P>Ca>Mg>Fe>Na>Zn >Cu. The unsaturated fatty acid content was 96.71-96.94%, and the major fatty acids oleic acid and linoleic acid were present. Total sugar content was 15.00-26.17 mg glucose/g. The acidic polysaccharide content was 0.56-0.80 mg ${\beta}$-Dgalacturonic acid/g. These results showed the differences in the physicochemical characteristics of ginseng seeds with respect to cultivation location, cultivation year, and species.

Discrimination of Atractylodes Rhizome White Using Anatomical Characteristics and SCAR Markers (해부형태적 특징과 SCAR Marker를 이용한 백출의 기원식물 판별)

  • Bang, Kyong-Hwan;Sung, Jung-Sook;Park, Chung-Heon;Jin, Dong-Chun;Park, Chun-Geon;Yu, Hong-Seob;Park, Hee-Woon;Seong, Nak-Sul
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.1
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    • pp.53-59
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    • 2004
  • Finding a means to discriminate the commercial herb medicines when they were dried and sliced is a very important and imminent project in Korea. To differentiate plant origins and the commercial herb medicines of Atractylodes japonica and A. macrocephala, two discriminative methods using anatomical characteristics and SCAR marker were applied. It was possible to discriminate plant origins and the commercial herb medicines between A. japonica and A. macrocephala by anatomical characteristics: development of periderm, layer of stone cell, distribution of laticiferous vessels, development of xylem fiber in xylem ray, contained quantity of clustered crystals and others. While, two SCAR markers were developed from RAPD clones: SAjR2 (600 bp) from AjR2 and SAmR1 (1,200 bp) from AmR1. These two markers were enough for discrimination plant origins and the commercial herb medicines between A. japonica and A. macrocephala. The result of application of anatomical characteristics and SCAR markers to investigate current status in domestic herb market, Daegu and Kumsan herb market, it was identified to be current herb medicines of A japonica.