• Journal of the Korean Wood Science and Technology
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• 제46권2호
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• pp.125-131
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• 2018

This study was conducted to understand and to compare the qualitative aspects of anatomical characteristics in compression wood (CW), lateral wood (LW), and opposite wood (OW) in a stem of Ginkgo biloba. The qualitative analysis was examined by optical microscopy and scanning electron microscopy. CW in Ginkgo biloba disks were dark brown in color, and the OW and LW were light brown in color. CW and OW showed abrupt transitions from earlywood to latewood, while LW showed a gradual transition. Cross sections of CW presented circular tracheids with angular outlines, many intercellular spaces, and varying sizes of lumens. Cross sections of LW and OW showed rectangular tracheids, fewer intercellular spaces, and varying sizes of lumens. In radial sections, CW showed an irregular arrangement of tracheids in earlywood, while LW and OW showed comparatively regular arrangements. Slit-like bordered pits on the tracheid cell wall, piceoid pits in the crossfield, and a few spiral checks were present in CW. LW and OW showed bordered pits with slightly oval-shaped apertures, as well as cuppresoid pits in the crossfields. Rays were primarily uniseriate, with few biseriate rays in the tangential sections of CW, LW, and OW. The tips of the tracheids were branched in CW but had a normal appearance in LW and OW.

• 한국산림과학회지
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• 제89권4호
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• pp.527-535
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• 2000

감자전분젤을 매개로 한 수평식 전기영동장치를 이용하여 은행나무(Ginkgo biloba L.)의 megagametophyte로부터 15개 동위효소의 변이가 분석되었다. 분석된 효소 가운데서 ADH, G6PD, IDH, MPI, UGPP의 5개 효소에서는 변이가 나타나지 않았으며, 나머지 10개 효소의 11개 동위효소 구역(ACON-A, FST-B, GDH-A, GOT-B, MDH-B, MDH-C, MNR-A, PGI-B, PGM-A, 6PGD-B, SKDH-B)에서 다형성이 관찰되었다. 이중 MDH-B를 제외한 모든 구역에서 관찰된 동위효소 변이들이 1 : 1의 독립적 분리비를 보임으로써, 이들이 단일 유전자좌에 의해 조절되는 공우성 대립유전자임을 추정할 수 있었다. 한편, 동위효소 유전자좌의 3가지 조합(ACON-A : MDH-B, GOT-B : PGI-B, MNR-A : SKDH-B)에서 약한 연관관계가 관찰되었으며, 이들의 재조합 비율은 0.38-0.40로 계산되었다 (p<0.05).

• Journal of Korean Neurosurgical Society
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• 제49권1호
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• pp.13-19
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• 2011

Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.

• 생약학회지
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• 제39권3호
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• pp.218-222
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• 2008

This study was carried out to establish standard analytical methods for Ginkgo biloba leaf extract. Ginkgo flavonoids, terpene lactones, ginkgolic acids were employed as reference compounds for analytical method. Analytical method of US Pharmacopoeia was adopted for flavonoids and terpene lactones, and a new method was developed for ginkgolic acids. Analytical methods established in this study could be applied to a reasonable and unified quality control of G. biloba leaf extract.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.237-244
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• 1998

A study was carried out to elucidate the relation between the production of flavonol glycosides and the change of phenylalanine ammonia-lyase activity in cell suspension cultures of Ginkgo biloba by the unassisted and synergistic effects of various factors. The quercetin production showed a mixed-growth-associated pattern in cell suspension cultures. Fluorescent light and UV radiation increased phenylalanine ammonia-lyase (PAL) activity, and resulted in the increase of the production of quercetin and kaempferol ten- and four-fold, respectively, as compared to that obtained in the normal culture condition. The cell growth of Ginkgo biloba was enhanced .at higher temperatures whereas the quercetin production was at its maximum at low temperatures. Moreover, the quercetin production was increased by temperature change during the culture period. In particular, the quercetin production was at the highest level when the culture temperature was elevated from $10^{\circ}C\;to\;30^{\circ}C$. The addition of phenylalanine as a precursor in the culture medium stimulated an 8-fold increase in the production of quercetin; the addition of naringenin caused a l0-fold increase. The quercetin production was also greatly increased by feeding enzyme cofactors such as 2-ketoglutarate and ascorbic acid in the culture medium, but specific PAL activity was not increased except with phenylalanine feeding. The synergistic effect of UV radiation and naringenin feeding was observed, resulting in the increase of flavonol glycoside production at a rate higher than in any other case investigated.

• 약학회지
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• 제49권2호
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• pp.140-146
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• 2005

Candida albicans, a polymorphic fungus, causes systemic and local infections. Recent reports show that the fungus is a main etiological agent for the arthritis. For trea tment, antifungal drugs and/or rheumatoid drugs are used, but resistance and side effects limit application of the drugs. In search of new sources for treatment of the fungal arthritis, we choose Egb 761 (extract of Ginkgo leaves 761), one of the most popular over-the-counter herbal medicines. The Egb 761 contains two major ingredients such as terpene and flavonoid. In the present study, we examined if the terpene portion of Egb 761 had anti-inflammatory activity against C.albicans-caused arthritis. The terpene was extracted with combination of methanol and water from the Egb 761, followed by gel-permeation chromatography. Presence of terpene was determined by the Salkowski colorimetric method and HPLC analysis. For an animal model of inflammation induction, mice were given an emulsion form of C.albicans cell wall mixed with Complete Freund's Adjuvant (CFA) by footpad-injection. Results showed that intraperitoneal administration of the water-soluble portion that contained terpene and flavonoid reduced the inflammation. Whereas the terpene had anti-inflammatory activity, flavonoid portion had no such activity, For determination of possible mechanism of the activity, the terpene seemed to be suppression of nitric oxide (NO) production from LPS-treated macrophages. Taken together the Ginkgo terpene may have anti-inflammatory effect against C.albicans-caused arthritis, possibly by blocking NO production.

• The Korean Journal of Physiology
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• 제29권1호
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• pp.61-67
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• 1995

The extract of Ginkgo bibloba (EGb) is a complex mixture of natural products from the Ginkgo leaves and clinically used for the treatment of cerebral and peripheral circulatory disturbances due to its combined activity of several vasoactive principles. In this study we investigated the action of EGb and its mechanism in isolated rabbit corporal smooth muscle to evaluate the possibility of using this material as a pharmacoerecting agent. Strips of rabbit corpus cavernosum were mounted in organ chambers to measure isometric tension. EGb began to exert an relaxing effect at 1 mg/ml in the submaximally precontracted muscle strips with phenylephrine $(PHE,\;5{\times}10^{-6}\;M)$; causing concentration-dependent relaxation with maximal effect at $3{\sim}5\;mg/ml$. That relaxation was partially inhibited by removal of the smooth muscle endothelium or by pretreatment with a NO scavenger, pyrogallol $(10^{-4}\;M)$ or the guanylate cyclase inhibitor, methylene blue $(10^{-4}\;M)$. Pretreatment with EGb (3 mg/ml) inhibited PHE- $(5{\times}10^{-6}\;M)$ or KCI- (20 and 40 mM) induced contraction of muscle strip. In calcium-free high potassium solution EGb depressed the basal tone of the depolarized muscle strip and inhibited calcium-induced contraction when $CaCl_2$ $(10^{-4}\;M)$ was added. These results suggest that EGb relaxes rabbit corpus cavernosal smooth muscle through multiple action mechanisms that include increasing the release of nitric oxide from the corporal sinusoidal endothelium, sequestration of intracytosolic calcium, and maybe a hyperpolarizing action.

• Journal of the Korean Wood Science and Technology
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• 제21권2호
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• pp.39-48
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• 1993

End-matched disks of 7.7cm thickness were part-time dried in vacuum press dryer to investigate the drying characteristics by using drying schedules and end treatments. The ginkgo disks could be dried faster with severer end checks and ring failures by C schedule than by S schedule. There were no significant differences in drying rates and amounts of end checks, ring failures and V-shaped checks occurred between ginkgo disks end-taped with Korean traditional paper (KTP) and those untreated. The drying time of walnut disks was slightly reduced by end-taping with KTP. And the length of end checks and V-shaped checks and the frequency of defected disk were decreased more effectively than those untreated. The tangential strains and tangential drying stresses near bark sides of ginkgo and walnut disk untreated were developed in the early stage of drying and incresed slightly curvi-linearly above fiber saturation point (fsp) and more curvi-linearly below fsp. The influence of end-taping with KTP on the drying rate for impermeable walnut disk was stronger than that for permeable ginkgo disk.

• 한국산림과학회지
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• 제73권1호
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• pp.55-62
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• 1986

본(本) 연구(硏究)는 잣나무와 은행나무에 대하여 인공산성우(人工酸性雨), 건조(乾燥) 및 식염수(食鹽水)를 처리(處理)하여 가시적(可視的) 엽피해(葉被害)를 유발(誘發)시켰을 때 수종간(樹種間) 및 처리간(處理間)의 엽피해(葉被害)에 대한 조직변화(組織變化)를 알아보고자 시도(試圖)되었다. 잣나무의 피해엽조직(被害葉組織)에서 공통적(共通的)으로 관찰(觀察)된 것은 엽육조직(葉肉組織)의 파괴(破壞)였으며, 산성우(酸性雨)에 의한 피해엽조직(被害葉組織)에서는 표피(表皮)가 파괴(破壞)되었으며, 건조(乾操)에 의한 피해엽조직(被害葉組織)에서는 표피(表皮), 엽육조직(葉肉組織) 및 내피(內皮)가 수축되거나 변형(變形)되었으며, 식염수(食鹽水)에 의한 피해(被害)는 사부(篩部)와 이입조직(移入組織)(transfusion tissue)의 파괴(破壞) 등이 각각 특이(特異)한 점으로 나타났다. 한편, 은행나무 피해엽조직(被害葉組織)에서 공통적(共通的)으로 관찰(觀察)된 것은 해면조직(海綿組織)의 파괴(破壞)였으며 산성우(酸性雨)에 의한 피해(被害)로는 표피(表皮)와 책상조직(柵狀組織)의 파괴(破壞), 건조(乾操)에 의 한 피해(被害)로는 해면조직(海綿組織)의 수축 변형(變形), 식염수(食鹽水)에 의한 피해(被害)로는 내피(內皮)와 이입조직(移入組織)의 가벼운 비대현상(肥大現象)과 파괴(破壞) 등이 각각 특이(特異)한 점이었다.

• 한국가구학회지
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• 제23권1호
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• pp.95-102
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• 2012

The objective of this study was to conduct the species identification and tree-ring dating of Lotus Pedestal of the Amitabha Statue at Ssangbong-Temple in Hwasun. The six wood blocks used for the Lotus Pedestal were hard pines (Pinus spp.; diploxilon) except one piece which was ginkgo (Ginkgo biloba L.). The lotus leaves surrounding the pedestal body were also made of ginkgo. Tree-ring patterns of 3 blocks were synchronized and a 133 years chronology was made. The chronology was crossdated well with the master chronology of Japanese red pine in South Korea. It dated back to A. D. 1551~1683, i.e. the last ring dated A. D. 1683. Through the estimation of the number of sapwood rings removed during carving, the felling year was calculated A. D. $1704{\pm}10$. The calligraphic writing on the Pedestal indicated that this statue was made in A. D. 1694. Therefore, the accuracy of the tree-ring dating has been proven.