• Journal of dental hygiene science
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• v.14 no.2
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• pp.150-157
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• 2014

The aim of this study was to suggest a convenient method of monitoring the gingival state through plaque detection. Quantitative Light-Induced Fluorescence-Digital (QLF-D), which can assess mature plaque, can be used to assess the oral hygiene status of individuals and to establish an adequate intervention plans for them. The subjects of the study participated in the clinical training at Department of Dental Hygiene, N University. The subjects completed questionnaires on general characteristics and oral hygiene methods. Then, photographs of maxillary and mandibular anterior teeth of the subjects were taken using the QLF-D. After the oral examinations, gingival state was recorded according to the $L{\ddot{o}}e$ & Silness's Gingival Index (GI). In addition, a plaque control record was calculated in percentage using disclosing agent. The analysis of the relation between the plaque control record and the QLF-D scores showed positive correlation (r=0.638, p<0.001), and the analysis of the relation between the QLF-D scores and the gingival bleeding index scores also showed positive correlation (r=0.562, p<0.001). Besides, the study classified the participants into healthy gingiva group and the gingivitis group according to the classification criteria of GI, and when the difference of QLF-D scores between the groups was analyzed, the QLF-D scores were statistically significantly higher (t=-2.785, p=0.007) in the gingivitis group ($1.71{\pm}1.545$) than the healthy gingiva group ($0.74{\pm}1.290$). When the differences in mean values of the QLF-D scores were analyzed against and the gingival bleeding index scores, the QLF-D scores of 0 and 1 belonged to the category of normal gingival state while the QLF-D scores of 2, 3 and 4 belonged to the category of gingivitis state. Therefore, it is deemed that the red fluorescence detected by the QLF-D from the mature supragingival plaque can be used for monitoring the state of gingivitis.

• Journal of the Korean Academy of Esthetic Dentistry
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• v.8 no.1
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• pp.28-35
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• 1999

The preservation of a healthy periodontal attachment is the most significant factor in the long-term prognosis of a restored tooth. The 'Biologic Width' is composed of the connective tissue attachment and the epithelial attachment in the dentogingival junction. The violation of the biologic width may result in a progressive inflammatory process and crestal bone loss. So a careful soft tissue management is needed to preserve it for the gingival health and an esthetic restoration. The following clinical cases show the five different situations of the violation of the biologic width and their management.

• Journal of Korean society of Dental Hygiene
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• v.21 no.5
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• pp.567-573
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• 2021

Objectives: This study aimed to determine the association between the oral health behavior of adolescents, symptoms of awareness and subjective happiness. Methods: This study is the 16th (2020) primitive self-administered survey that is completed using data on youth health behavior 54,948 to finally select people. Acomposite sample analysis was performed using SPSS window program 21.0. A chi-square test was performed tocompare subjective happiness according to the general characteristics of the subjects, oral health behavior, and awareness symptoms. Logistic regression analysis of the subjective happiness factor was the impact on composite samples. Results: Oral health is a subjective factor that affects happiness. Happiness was found to be 1.479 times higher when participants brushed their teeth more than three times a day and 1.175 times higher when they brushed after lunch. However, subjective happiness was 0.901 times lower when the awareness symptoms of pain was experienced, 0.843 times lower when there was tingling and throbbing, and 0.841 times lower when there was gingival pain. Conclusions: It is necessary to develop and guide various activity programs so that youth oral health education and subjective feelings of happiness can be improved to promote oral health.

• Journal of Korean society of Dental Hygiene
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• v.1 no.1
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• pp.133-142
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• 2001

The authors have studied the 8 weeks clinical examination On 32 patients of age 20s for experimental group and 34 patients of the same ages for control group, in order to find out the effect of plaque removal effect and gingival effect by use of the dentifrice contained with the Artemisiae Capillaris extract(dimethylesculetin) and NaCl. The obtained results are as followings: 1. Plaque removal effect is better at the experimental group than at the control group on 2 and 8 weeks(p<0.05). 2. There are not significantly differences on the calculus index and stain index between the experimental group and control group, during the 8 weeks experiment(p>0.05). 3. At the 8 weeks experiment, it revealed the less average scores on the PMA index for experimental group, than that of the control group(p<0.05).

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.4
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• pp.743-750
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• 1997

Gingivitis is the most prevalent type of periodontal disease and the dental plaque is considered as a major contributory factor. As the poor oral hygiene is firmly related to the occurrence of periodontal disease, pediatric dentist should make every effort to promote the oral health and control the plaque effectively for the high risk patients, especially for those who are under orthodontic treatment. P.M.T.C.(Professional Mechanical Tooth Cleaning), introduced by Dr. P. Axelsson in 1969, is a very effective method of plaque removal and can be performed by specially trained personnel. Two pediatric orthodontic patients were treated by P.M.T.C. for the elimination of gingivitis and gingival swelling. Signi ficant improvements of gingival condition were achieved in both cases but additional preventive programs and home care along with professional office care seem to be necessary for the best result.

• Journal of Korean society of Dental Hygiene
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• v.23 no.1
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• pp.73-82
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• 2023

Objectives: This study aimed to evaluate the oral symptoms experiences of Korean adolescents among multicultural families. Methods: Data on 985 participants from the 16th Korea Youth Risk Behavior Web-based Survey were collected and analyzed using complex sample descriptive statistics. Results: The foreign-born mother, foreign-born parents, and foreign-born father rates were 77.2%, 17.3%, and 5.4%, respectively. The toothache, gingival bleeding, tooth fracture, and tooth sensitivity experience rates were 21.3%, 18.8%, 13.3%, and 30.4%, respectively. The tooth fracture rate was highest in the foreign-born parents group (24.6%). The toothache odds ratio (OR) was 1.71 (95% CI: 1.24-2.37) times higher in girls. The foreign-born mother group was 2.47 (95% CI: 1.09-5.60) times higher than that of the foreign-born father or both parents foreign-born groups. The smoking group was 2.03 (95% CI: 1.20-3.43) times higher than that of the non-smoking group. The gingival bleeding OR was 5.11 (95% CI: 1.80-14.53) times higher in the lowest economic status group. The tooth fracture OR was 3.44 (95% CI: 1.01-11.70) times higher in the lowest economic status group. The tooth sensitivity OR was 1.53 (95% CI: 1.14-2.04) times higher in girls. Conclusions: It is necessary to establish a program to promote oral health of adolescents from multicultural families.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.4
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• pp.678-685
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• 2006

A certain width of attached gingiva is required to maintain gingival health The purpose of this study was to examine the dimensional changes in the width of attached gingiva and the depth of gingival sulcus among the deciduous, mixed and permanent dentitions and establish baseline information on the width of attached gingiva in Korean children. Eighty-eight children aged 4 to 14, who visited the Department of Pediatric Dentistry at Samsung Medical Center, were selected and divided into 3 groups according to the periods of dentition: deciduous, mixed and permanent dentitions. The width of keratinized gingiva and the depth of gingival sulcus were measured in each group with a periodontal probe and the width of attached gingiva was determined. The width of attached gingiva in maxillary and mandibular first molars increased significantly with age after eruption in the permanent dentition (p<0.05). The sulcus depth significantly increased in newly erupted permanent teeth with narrower width of attached gingiva (p<0.05) in all of the experimented teeth with the exception of the mandibular central incisor during the transition period. The results suggest that the mean width of attached gingiva does not increase steadily from the deciduous to the permanent dentition.

• The Journal of Korean Medicine
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• v.31 no.2
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• pp.91-108
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• 2010

Background & Objective: The aim of this study was to investigate the effect of P. radix on the inflammatory related gene expression in IL-$1{\beta}$-stimulated primary human gingival fibroblast using Whole Transcript Sense Target (WT-ST). Method: Human gingival fibroblast was incubated with P. radix [100 or $200\;{\mu}g/ml$], and IL-$1{\beta}$ [$1ng/m{\ell}$] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling $Assay^{(R)}$. Result: In the DEG results, 782 genes were up-regulated in the IL-$1{\beta}$-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-$1{\beta}$ and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-$1{\beta}$-treated when compared to IL-$1{\beta}$-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-$1{\beta}$-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix $200{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group appear to be more involved in the metabolism-related pathways than in the $100{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group. Conclusion & Discussion: By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.

• Journal of Periodontal and Implant Science
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• v.35 no.3
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• pp.649-659
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• 2005

The purpose of this study was to quantify and compare the level of MMP-1 in the healthy or inflamed gingival tissue of patients with or without type 2 diabetic mellitus. We investigated whether mean amount of MMP-1 was changed by chronic periodontitis and type 2 DM. Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was divided into the three group. Group 1(n=8) was clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2(n=8) was inflamed gingiva from patients with chronic periodontitis. Group 3(n=8) was inflamed gingiva from patients with chronic periodontitis and type 2 diabetes. Tissue samples were prepared and analyzed by Western blotting. The quantitative analysis of MMP-1 was performed using a densitometer and statistically analyzed by ANOVA. MMP-1 was expressed in all samples and an increased MMP-1 level was observed in group 2 compared to group 1 and decreased MMP-1 level was found group 3 compared to group 2, but the differences among 3 groups were not statistically significant. In conclusion, this study demonstrated that MMP-1 levels of inflamed gingiva of systemically healthy patient(group 2) were higher than normal gingiva of systemically health patients and although the severity of gingival inflammation in group 2 and 3 were similar, MMP-1 expression was decreased in diabetic patients than systemically healthy periodontal patients.

• Journal of Periodontal and Implant Science
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• v.49 no.5
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• pp.270-286
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• 2019

Purpose: Despite the well-known anti-inflammatory effects of vitamin D in periodontal health, its mechanism has not been fully elucidated. In the present study, the effect of vitamin D on strengthening E-cadherin junctions (ECJs) was explored in human gingival keratinocytes (HGKs). ECJs are the major type of intercellular junction within the junctional epithelium, where loose intercellular junctions develop and microbial invasion primarily occurs. Methods: HOK-16B cells, an immortalized normal human gingival cell line, were used for the study. To mimic the inflammatory environment, cells were treated with tumor necrosis factor-alpha ($TNF-{\alpha}$). Matrix metalloproteinases (MMPs) in the culture medium were assessed by an MMP antibody microarray and gelatin zymography. The expression of various molecules was investigated using western blotting. The extent of ECJ development was evaluated by comparing the average relative extent of the ECJs around the periphery of each cell after immunocytochemical E-cadherin staining. Vitamin D receptor (VDR) expression was examined via immunohistochemical analysis. Results: $TNF-{\alpha}$ downregulated the development of the ECJs of the HGKs. Dissociation of the ECJs by $TNF-{\alpha}$ was accompanied by the upregulation of MMP-9 production and suppressed by a specific MMP-9 inhibitor, Bay 11-7082. Exogenous MMP-9 decreased the development of ECJs. Vitamin D reduced the production of MMP-9 and attenuated the breakdown of ECJs in the HGKs treated with $TNF-{\alpha}$. In addition, vitamin D downregulated $TNF-{\alpha}$-induced nuclear factor kappa B ($NF-{\kappa}B$) signaling in the HGKs. VDR was expressed in the gingival epithelium, including the junctional epithelium. Conclusions: These results suggest that vitamin D may avert $TNF-{\alpha}$-induced downregulation of the development of ECJs in HGKs by decreasing the production of MMP-9, which was upregulated by $TNF-{\alpha}$. Vitamin D may reinforce ECJs by downregulating $NF-{\kappa}B$ signaling, which is upregulated by $TNF-{\alpha}$. Strengthening the epithelial barrier may be a way for vitamin D to protect the periodontium from bacterial invasion.