• Title/Summary/Keyword: Gill environment

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Hematological constituents and ultrastructural changes in dark-banded rockfish, Sebastes inermis, under nitrite stress

  • Park, In-Seok;Goo, In Bon;Kim, Young Ju;Choi, Jae Wook;Oh, Ji Su
    • Journal of fish pathology
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    • v.26 no.1
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    • pp.1-9
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    • 2013
  • The acute toxicity and sublethal effects of nitrite on the dark-banded rockfish, Sebastes inermis (mean body weight: $83.3{\pm}7.2$ g), were studied under static conditions for a period of 96 h. The acute toxicity of nitrite was at the 50% lethal concentration ($LC_{50}$) of 700 mg/L. The sublethal effects on selected hematological parameters of the dark-banded rockfish, such as its osmolality, hematocrit, cortisol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were measured after 0, 6, 12, 24, 48, 72, and 96 h of exposure to 0, 50, 100, 200, 400, or 700 mg/L nitrite. Sublethal nitrite caused a progressive reduction in the hematocrit of the fish, depending on the nitrite concentration and the exposure period. Exposure to 100-700 mg/L nitrite for 96 h caused a reduction in the hematocrit and an increase in cortisol, ALT, and AST compared with the control levels. Abnormal ultrastructural changes in the gills and liver tissues were observed in fish exposed to 700 mg/L nitrite for up to 96 h compared with the control tissues. Ultrastructural changes included atrophic gill mitochondria and hepatocytes that developed smooth endoplasmic reticulum and atrophic mitochondria. Although no rockfish mortality occurred at 500 mg/L nitrite, all the hematological parameters examined responded adversely to a nitrite dose of 200 mg/L for 96 h. These results show that although the acute toxic concentration of nitrite for the dark-banded rockfish is > 700 mg/L, sublethal concentrations of nitrite also negatively affect its hematological parameters.

Studies on the Infestation of Monogenean Helminth, Microcotyle sebastis, and Copepod, Clavella adunca from Sebastes melanops and S. maliger (볼락류(Sebastes melanops, S. maliger)로부터 검출된 단생흡충류 Microcotyle sebastis와 요각류 Clavella adunca 감염에 관한 연구)

  • Chun, Kae-Shik
    • Journal of Environmental Health Sciences
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    • v.29 no.1
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    • pp.16-18
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    • 2003
  • Seven specimens of Sebastes melanops, black rockfish and three of S. maliger, quillback rockfish, were collected from Oregon Coast Aquarium during the period of September 19 to November 5, 2001 and examined for parasites. One monogenean helminth species and parasitic copepod species were recorded from black and quillback rockfishes, respectively. A total of 26 parasites were collected: 16 Microcotyle sebastis from the gill filaments of black and quillback rockfishes and 10 Clavella adunca were found in the fins of black rockfish. The prevalence of Microcotyle sebastis was 40.0%, and intensity of infection was 2.0(1-11). With regard to Clavella adunca, the prevalence and intensity were 20.0% and 1.4(2-8), respectively.

Development of a Holographic Interferometric Tomography System and Its Application to Three-Dimensional Natural Convection (홀로그래피 간섭 토모그래피 개발 및 3차원 자연대류 열전달에의 적용)

  • Lee, Soo-Man;Kang, Min-Gu;Cha, Dong-Jin;Joo, Won-Jong;Kang, Bo-Seon
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.1609-1614
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    • 2003
  • In this study, a practical holographic interferometric tomography system, which is instantaneous and non-contact for measuring three dimensional flow field, was developed. The system consists of holographic recording/reconstruction system, fringe analysis code and computational tomography code and it is developed with Gill environment for general users. The developed system was applied to three-dimensional natural convection from a discrete flush-mounted circular heat source on the bottom of a cubic enclosure. The heat source was located at the off-center of the bottom plate so that three-dimensional temperature field can be achieved. A set of multi-directional holographic interferograms was recorded by employing a double-reference beam, double-exposure holographic technique. Optical pathlength data were extracted from the recorded interferometric data and finally three dimensional temperature field inside the cube was reconstructed.

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TEM Observations of Chemosynthetic Bacteria in the Deep-sea Hydrothermal Vents and Seep Organisms

  • Kim, Dong-Sung;Ohta, Suguru
    • Ocean and Polar Research
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    • v.24 no.3
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    • pp.215-223
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    • 2002
  • Symbiosis of chemoautrophic bacteria with the members of hydrothermal vent and cold seep communities in the deep-sea were examined by histology using transmission electron microscopy; Bathymodiolus spp. from Sagami Bay, the Iheya Ridge and the North Fiji Basin; and Ifremeria nautilei from the North Fiji Basin. Two species of Bathymodiolus, each from Sagami Bay and the Iheya Ridge harbored methane-oxidizing symbionts within their gill tissues. Vent gastropod Ifremeria nautilei from the hydrothermal vents of the North Fiji Basin housed two types of symbionts; one sulfur-oxidizing type and the other methane-oxidizing type. The occurrence of chemosynthetic symbionts in these organisms were expected before-hand based on the ecological observations of their habit. The other members of these groups from world oceans and the recent advances in the symbiosis of the vent and seep communities were reviewed.

Biosorption Technology Using Algal Biomass in Canadian Environment Industries (캐나다 환경산업의 조류 바이오매스 이용 생흡착 기술)

  • Volesky, Bohumil;Kong, Surk-Key
    • Proceedings of the Korean Institute of Resources Recycling Conference
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    • 2005.11a
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    • pp.3-14
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    • 2005
  • We Investigated minutely how the biosorption technology using algal biomass is opened in Canadian technology society. Making comparative study for relative technologies in views of overall unit operation cost, we could grasp next facts. - Algal biomass plays the competitive performance for various metals. - Algal biomass biosorbent is regenerated. - Reactor system is not and involved one. This means that algal biomass occupies the strong position as biosorbent. Especially, in North America, for the purpose of metal bearing wastewater treatment, 20 hundred million US dollars was appropriated a sum for the purchase of ion exchange resin. But it is only thirty million US dollars if algal biomass biosorbent is used on behalf of ion exchange resin. Furthermore, the expenses for same treatment can be cut down additively through metal recovery.

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Absorption and Fluorescence Spectra of Dy(Ⅲ) Complexes with Some Terdentate Ligands

  • Jung Seung Hee;Yoon Soo Kyung;Kim Jong Goo;Kang Jun-Gill
    • Bulletin of the Korean Chemical Society
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    • v.13 no.6
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    • pp.650-654
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    • 1992
  • Absorption and fluorescence spectra are reported for four different 1 : 3 $Dy^{3+}$ : ligand systems in aqueous solution under mild alkaline pH conditions. The ligands included in this study are oxidiacetate, dipicolinate, iminodiacetate and methyliminodiacetate. The oscillator strengths for the 4f→4f multiplet-to-multiplet transitions are empirically determined from the absorption spectra and the intensity parameters ${\Omega}_{\lambda}$}(${\lambda}$ = 2, 4, 6) for the systems are also obtained by applying the Judd-Ofelt theorem to the observed oscillator strengths. The values of the intensity parameters for the systems are compared and discussed in terms of ligand structural properties to investigate how the intensity parameters can response to the minor changes in the ligand environment. In addition, the relative oscillator strengths for fluorescence are evaluated and compared to the results obtained from absorption spectra.

Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss

  • Kim, Na Na;Choi, Young Jae;Lim, Sang-Gu;Kim, Bong-Seok;Choi, Cheol Young
    • Ocean and Polar Research
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    • v.38 no.2
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    • pp.103-113
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    • 2016
  • This study aimed to examine the role of two aquaporin isoforms (AQP3 and AQP8) in response to the hyperosmotic challenge of transitioning from freshwater (FW) to seawater (SW) during parr and smoltification (smolt) using the rainbow trout, Oncorhynchus mykiss. We examined the changes in the expression of AQPs mRNAs in the gills and intestine of the parr and smolt stages of rainbow trout transferred from FW to SW using quantitative real-time PCR in an osmotically changing environment [FW, SW, and recombinant AQP3 (rAQP3) injection at two dosage rates]. Correspondingly, AQPs were greater during smoltification than during parr stages in the rainbow trout. Plasma osmolality and gill $Na^+/K^+$-ATPase activity increased when the fish were exposed to SW, but these parameters decreased when the fish were exposed to SW following treatment with rAQP3 during the transition to seawater. Our results suggest that AQPs play an important role in water absorbing mechanisms associated with multiple AQP isoforms in a hyperosmotic environment.

Contributors to Fatigue of Mine Workers in the South African Gold and Platinum Sector

  • Pelders, Jodi;Nelson, Gill
    • Safety and Health at Work
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    • v.10 no.2
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    • pp.188-195
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    • 2019
  • Background: Mine workers in South Africa face challenges relating to poor health and safety, including fatigue risks, and poor socioeconomic and living conditions. Fatigue results in impaired mental and physical performance. The aim of this study was to assess contributors to fatigue of mine workers in South Africa. Methods: Data collection took place at four gold mines and one platinum mine in South Africa. A total of 21 focus groups were held with individuals in management, union representatives, and mine workers, and 564 questionnaires were completed by mine workers to gather information about fatigue and potential contributors to fatigue at these mines. Results: Qualitatively (through focus groups), fatigue was attributed to extended working hours, harsh working conditions, high workloads, production pressure, and resource constraints, along with aspects relating to demographic and socioeconomic factors, living conditions, lifestyle, health, and wellness. Greater fatigue was significantly associated with younger age, indebtedness, a lack of exercise, poor nutrition, less sleep, increased alcohol use, poor self-reported health, more sick leave, higher stress, and lower job satisfaction. Conclusion: The aim of the study was achieved; numerous work-, sociodemographic-, lifestyle-, and wellness-related factors were linked to fatigue in the participating mine workers. Contributors to fatigue should be addressed to improve health, safety, and sustainability in the industry.

Comparison of the Spawning Characteristics of the Yellow Striped Flounder Pseudopleuronectes herzensteini in the Coastal Waters off Gangwon and Gyeongbuk, Korea (동해안에 서식하는 참가자미(Pseudopleuronectes herzensteini)의 해역별 산란특성 비교)

  • Kim, So Ra;Lee, Soo Jeong;Yang, Jae Hyeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.3
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    • pp.318-328
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    • 2021
  • We investigated the maturity and spawning of the brown sole Pseudopleuronectes herzensteini, in the coastal waters of the East Sea off Gangwon and Gyeongbuk, South Korea, using samples collected by gill net and longline fishery from January 2018 to December 2019. We analyzed oocyte development, monthly maturity stage, gonadosomatic index (GSI) and total length at maturity. Histological analysis of ovarian development in P. herzensteini revealed that the development was group-synchronous. The spawning peak in Gangwon and Gyeongbuk occurred from April to May and from February to March, respectively. Thus, the spawning season in Gangwon was 1-2 months later than that in Gyeongbuk. The length at 50% maturity for females was estimated as 24.2 cm and 19.0 cm in Gangwon and Gyeongbuk, respectively. Similarly, the length at 50% maturity for males was estimated as 19.8 cm and 16.5 cm in Gangwon and Gyeongbuk, respectively.