• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.986-992
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• 2009

Type I interferons (IFNs) are critical mediators of the innate immune system to defend viral infection. Interferon regulatory factor (IRF) and signal transducer and activator of transcription (STAT) play critical roles in type I IFN production in response to viral infection. Luteolin is natural polyphenolic compounds that have anti-inflammatory, cytoprotective and anti-carcinogenic effects. However, the mechanism of action and impact of luteolin on innate immunity is still unknown. In this study, we examined the effects of luteolin on the lipopolysacchride (LPS)-induced inflammatory responses. Luteolin inhibited Type I IFNs expression of mRNA and increased interleukin(IL)-10 expression of mRNA. Next, we examined the protective effects of IL-10 using IL-10 neutralizing antibody (IL-10NA). Blockade of IL-10 action didn't cause a significant reduction of Type I IFNs than LPS-induced luteolin pretreatment. Pretreatment of luteolin inhibited the level of IRF-1, and IRF-7 mRNA and the nuclear translocation of IRF-3. Also, luteolin reduced the activation of STAT - 1, 3. Theses results suggest that luteolin inhibits LPS-induced the production of Type I IFNS by both IRFs and STATs not IL-10 and may be a beneficial drug for the treatment of inflammatory disease.

• Journal of agricultural medicine and community health
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• v.40 no.4
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• pp.228-239
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• 2015

Objectives: The purpose of this study was to measure the symptom prevalence of acute pesticide poisoning among farmers in Gyeong-gi province and to investigate the occupational risk factors. Methods: The subjects were 663 farmers using pesticide directly or indirectly and the survey was performed during 2013. 7. 11 - 2014. 8. 27. The data were analyzed by SPSS 21.0. Results: The prevalence of acute pesticide poisoning was 6.63% and dizziness was the most common symptom. Acute pesticide poisoning was related with 'days of pesticide use per year', 'lifetime pesticide application days', 'the number of pesticide mixture' and 'type of farming'. Conclusions: It is necessary to manage and educate the pesticide poisoning-vulnerable group more systematically. Afterward additional study is needed to investigate the farming safety behavior and delicate amount of pesticide exposure.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.337-364
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• 2020

Mt. Taegi (1,261.4 m) located in Gangwon-do, is a geographically important region for plant biodiversity. But the biodiversity of Mt. Taegi has been seriously damaged by the invasion of foreign plants, the development of forests and so on. We conducted 21 field surveys between April and August in 2008 and March and October in 2018 to investigate the vascular flora in Mt. Taegi. Total 662 taxa were identified including 96 families, 338 genera, 580 species, 4 subspecies, 66 varieties and 12 formas. Among them, 28 taxa were Korean endemic plants and 8 taxa were rare plants designated by the Korea Forest Services, and 11 taxa were listed on the Red list of endangered plants and 127 taxa were designated as the floristic regional indicator plants according to the Ministry of Environment. The alien plants were 45 taxa, including R. acetosella that is the ecosystem disturbing species.

• Korean Journal of Food Science and Technology
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• v.45 no.2
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• pp.257-261
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• 2013

Rat pheochromocytoma cells (PC12) and mice were utilized as in vitro and in vivo models to determine the neuroprotective effects of a 70% acetone extract of black soybean seed coat (BSSCE). BSSCE showed higher total phenolic contents than other extracts. Intracellular reactive oxygen species accumulation from $H_2O_2$ treatment of PC12 cells was significantly reduced when BSSCE was present in the media compared to PC12 cells treated with $H_2O_2$ only. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT) reduction assay and lactate dehydrogenase assay also showed significantly increased protective effects in PC12 cells. In addition, BSSCE improved the in vivo cognitive ability against amyloid beta peptide-induced neuronal deficits.

• Journal of the Korean Applied Science and Technology
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• v.30 no.2
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• pp.280-289
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• 2013

Various vehicles have been studied in order to protect skin ageing and sustain constantly moisturization. Recently, in relation to maintain of moisture, absorption and penetration of active materials, there has been introducing many preparing methods such as liposome, liquid crystal and multilamellar emulsion. We developed multilayer lamellar vesicle using cetearyl alcohol/ceteth-20 phosphate/dicetyl phosphate as analogy of phospholipid according to variation of shear rate and pH. These multilayer lamellar vesicles were confirmed by cross polarizing microscope. As results, morphologies of lamellar vesicle were not uniformed at low shear rate and pH. Also, stabilities for encapsulation of retinol were observed at $42^{\circ}C$ during two months. As a result, quantitative content of retinol decreased at low pH. Multilayer lamellar vesicle decreased 14% of transepidermal water loss compared with O/W emulsion. We compared multilayer lamellar sun cream to O/W sun cream using in vitro SPF test of water resistance and concluded that multilayer lamellar sun cream is similar to O/W sun cream in water resistance.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.43 no.4
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• pp.229-238
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• 2017

Objectives: The primary purpose of this study was to investigate the factors related with additional administration of sedative agent during intravenous conscious sedation (IVS) using midazolam (MDZ). The secondary purpose was to analyze the factors affecting patient satisfaction. Materials and Methods: Clinical data for 124 patients who had undergone surgical extraction of mandibular third molar under IVS using MDZ were retrospectively investigated in this case-control study. The initial dose of MDZ was determined by body mass index (BMI) and weight. In the case of insufficient sedation at the beginning of surgery, additional doses were injected. During surgery, peripheral oxygen saturation, bispectral index score (BIS), heart rate, and blood pressure were monitored and recorded. The predictor variables were sex, age, BMI, sleeping time ratio, dental anxiety, Pederson scale, and initial dose of MDZ. The outcome variables were additional administration of MDZ, observer's assessment of alertness/sedation, intraoperative amnesia, and patient satisfaction. Descriptive statistics were computed, and the P-value was set at 0.05. Results: Most patients had an adequate level of sedation with only the initial dose of MDZ and were satisfied with the treatment under sedation; however, 19 patients needed additional administration, and 13 patients were unsatisfied. In multivariable logistic analysis, lower age (odds ratio [OR], 0.825; P=0.005) and higher dental anxiety (OR, 5.744; P=0.003) were related to additional administration; lower intraoperative amnesia (OR, 0.228; P=0.002) and higher BIS right before MDZ administration (OR, 1.379; P=0.029) had relevance to patient dissatisfaction. Conclusion: The preoperative consideration of age and dental anxiety is necessary for appropriate dose determination of MDZ in the minor oral surgery under IVS. The amnesia about the procedure affects patient satisfaction positively.

• Korean Journal of Materials Research
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• v.8 no.11
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• pp.1031-1037
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• 1998

To investigate the effect of cooling rate and annealing temperature on the corrosion of Zircaloy-4 and Zr-2. 5Nb alloys, autoclave corrosion tests were performed at $500^{\circ}C$ for the specimens prepared by various heat treatments. The specimens were heat-treated at $1050^{\circ}C$ for 30 minutes and cooled by ice-brine quenching, water quenching, oil quenching, air cooling, and furnace cooling. To investigate the effect of annealing temperature, the specimens were annealed at $\alpha$, ($\alpha$+$\beta$)-, and $\beta$-temperatures. It was observed from the $500^{\circ}C$ corrosion test that nodular corrosion occurred on the Zircaloy-4 alloy but did not occur on the Zr-2.5Nb alloy. The corrosion resistance of Zircaloy-4 increased with increasing the cooling rate. On the other hand, the corrosion resistance of Zr-2.5Nb decreased with increasing the cooling rate and the annealing temperature. It is suggested that corrosion resistance of Zircaloy-4 would be controlled by the distribution of Fe and Cr element in the matrix and precipitates, while that of Zr-2.5Nb alloy the niobium concentration and $\beta_{-Nb}$ phase.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.555-561
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• 1999

The development of sycamore lace bug, Corythucha ciliata, had been studied at four constant temperature levels of 15, 20, 25, and $30^{\circ}C$. And characteristics of its oviposition were studied at field. Developmental periods of eggs were 39.1, 17.1, 9.8, and 8.0 days, those of nymphs were 58.5, 23.8, 14.5, and 10.8 days, hatchabilities of eggs were 42.0, 78.5, 83.3, and 78.7%, and survival rates of nymphs were 14.7. 60.5, 75.7, and 48.9% at different temperatures of 15, 20, 25, and $30^{\circ}C$. Lower development threshold temperature and the effective accumulative temperature above the threshold required to complete development from egg to nymph were $11.5^{\circ}C$ and 344.8 degree days, respectively. The optimum temperature was estimated to be $25^{\circ}C$ for developments of egg and nymph. The longevities of adults were 41.0 days and 37.0 days for female and male, respectively.

• The Journal of Korean Medicine
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• v.25 no.3
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• pp.123-136
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• 2004

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won (WC) on the in vitro neuronal development and alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E/sub 18/ rat cortical cells were grown in a neurobasal medium containing B27 supplement and various concentration of WC. Initial development of growth cone was investigated by phase-contrast microscopy, while dendritic spine formation and synaptogenesis were investigated by immunocytochemistry with SynGAPα(a postsynaptic marker) and synaptophysin (presynaptic marker) antibodies. Alteration in gene expression was analyses by microarray using rat 5K-TwinChips. Results : WC suppressed the development of growth cones and WC increased the number of dendritic spines at 20 and 50㎍/mL concentration but there was no statistical significance. Instead, it significantly decreased the number at 100㎍/mL. The expression of anti-apoptosis gene Bcl2-like 1 (Bcl211) increased (Global M=0.46), while Akt1 decreased. Proapoptosis genes Bad and PDCD2 increased. The expression of hemoglobin alpha 1 (probably neuroglobin) increased (Global M=0.93). The expression of antioxidants such as catalase, heme oxygenase (HO), and PRKAG2 gene increased. The expression PKC gene increased. The expression of retinoic acid receptor alpha (RARα) increased significantly (Global M=1.0). Conclusions : These data suggest that WC trends to suppress cellular activity slightly in normoxia and increases the expression of apoptosis-, antioxidation-, oxygen capture-related genes in hypoxia, but increases Bcl111 that anti-apoptosis gene, on the other hand increases Bad, PDCD2 that pro-apoptosis genes, too..

• Journal of Korean Orthopaedic Sports Medicine
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• v.6 no.1
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• pp.45-49
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• 2007

Purpose: We analyzed the location and type of meniscus tear associated with ACL rupture in order to estimate and prepare whether the meniscus tear is in a repairable location. Materials and Methods: We reviewed 78 cases who had ACL reconstructive surgery due to ACL rupture. We set the period of acute injury on the basis of under 12 months after trauma. The location and type of meniscus tear was analyzed in accordance with MRI findings and arthroscopic findings. Results: The 50 cases of meniscus tear were detected out of the 78 ACL rupture;32 cases were lateral meniscus tears, 24 were medial meniscus tears and 6 cases were both menisci tears. From a total of 56 meniscus tears, 30 cases were longitudinal tears, 22 cases were red-red zone tears and 35 cases(62%) were posterior horn tears. Conclusion: The most common type of meniscus tear associated with ACL rupture war longitudinal tear at the red-red zone or meniscosynovial junction. Majority of the tears located at that place can be healed with conservative treatment, arthroscopic meniscus suture.