• Title/Summary/Keyword: Gil-ho Kim

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Evaluation of Susceptibility of Red Poultry Mite, Dermanyssus gallinae (Acari: Dermanyssidae) in Five Regions to 11 Acaricides (11종의 살비제를 이용한 5개지역 닭진드기의 감수성 평가)

  • Lee, Seung Ju;Yoon, Jong Ung;Park, Geun Ho;Kim, Hyun Kyung;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.56 no.4
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    • pp.427-434
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    • 2017
  • The susceptibility of Dermanyssus gallinae adults, collected from poultry farms in 5 regions, to 11 acaricides was investigated. When bifenthrin and formic acid with pyridaben were diluted 100 times, bifenthrin showed 100% acaricidal activity; however, formic acid with pyridaben showed less than 20% acaricidal activity for poultry farms in 3 regions (Gyeongju, Chilgok, and Geumsan) except Yeoncheon and Anseong. The carbamate compound, carbaryl showed 100% acaricidal activity in most of the regions, except Gyeongju. A phosphorus compound, dichlorvos showed 100% activity in most of the regions, yet 66.7% acaricidal activity in Yeoncheon. The susceptibility of D. gallinae from poultry farms in Yeoncheon and Anseong to most acaricides was high; however, in the other farms, the susceptibility of D. gallinae varied depending on the acaricide. Clothianidin, thiamethoxam, fenitrothion, and formic acid with pyridaben showed differences in acaricidal activity among regions. Therefore, farmers should concentrate during the selection of these acaricides. However, carbaryl, cartap hydrochloride, dichlorvos, and bifenthrin showed high activity against D. gallinae collected from poultry farms in five regions. Therefore, these acaricides could be used in most of these regions. To control D. gallinae effectively, the alternation of acaricides is necessary, and indiscriminate pesticide use should be avoided. Therefore, this study can serve as a basis for controlling D. gallinae.

Genotoxicity evaluation of balanced nutritional food for patients pasteurized by gamma irradiation at 4 kGy (4 kGy로 감마선 살균처리된 환자용 균형영양식의 유전독성 평가)

  • Song, Beom-Seok;Park, Jong-Heum;Kim, Jae-Kyung;Park, Ha-Young;Kim, Dong-Ho;Hong, Seong-Gil;Jeong, Sang-Hee
    • Food Science and Preservation
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    • v.24 no.1
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    • pp.100-106
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    • 2017
  • This study was conducted to evaluate the genotoxicity of balanced nutritional formular for patients containing various ingredients after gamma irradiation at 4 kGy. Since viable bacteria were not observed within the detection limit of 1 log CFU/g, a dose of 4 kGy was appropriate for the pasteurization of the formular. In a bacterial reverse mutation assay, both hot water and methanol extracts of the formular exhibited dose-independent responses, which was similar to those obtained from that of the negative control (distilled water or dimethyl sulfoxide). In a chromosomal aberration test using lung fibroblast cells of Chinese hamster, the numbers of normal chromosomes were comparable to those observed in the negative control, regardless of the treatment dose and metabolic activation system. Furthermore, no significant increases in the frequency of micronucleated polychromatic erythrocytes were observed relative to the control, when mice were fed with the formular at doses up to 2,000 mg/kg body weight. Therefore, the balanced nutritional formular for patients did not exhibit genotoxicity when pasteurization by gamma irradiation at 4 kGy.

Comparison of Quality of ESL and non-ESL Milk Depending upon Sensory Evaluation (관능검사를 통한 ESL(Extended Shelf Life)우유와 non-ESL우유의 품질 비교)

  • Han, Mi-Yeong;Jung, Byeong-Mun;Kim, Eung-Ryul;Kim, Wan-Sik;Jung, Hu-Gil;Chun, Ho-Nam;Yeo, Gyeong-Eun
    • Journal of Dairy Science and Biotechnology
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    • v.21 no.2
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    • pp.81-87
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    • 2003
  • This study was conducted to correlate the quality depending upon sensory evaluation of ESL(extended shelf life) and non-ESL milk during the shelf life. The most important quality assurance is sensory evaluation for dairy products. ESL and non-ESL milk were stored at $5^{\circ}C$ and $10^{\circ}C$ for 14 days. In order to compare physicochemical and sensory properties, pH, TA(titratable acidity), protein, fat, lactose, FFA(free fatty acid), and other organoleptic characteristics were measured. The results showed that physicochemical properties were not significantly changed during storage. On the other hand, the freshness was affected by storage conditions. The most freshness depending upon sensory evaluation was monitored at 5 days storage. Descriptive and acceptability analysis showed that more acceptable milk was ESL milk than non-ESL milk because of off-flavor and after taste of non-ESL milk. According to these results, it was shown that the freshness of milk products depends on sensory. And ESL milk was fresher than non-ESL milk after storage.

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Studies on the Production of Antibody to Mouse Zona Pellucida and its Effect on the Fertilization of Mouse Eggs (생쥐卵子의 透明帶에 대한 抗體의 生産과 이 抗體가 受精에 미치는 影響)

  • Seo, Gwang-Yeong;Lee, Seung-Bae;Choe, Gyeong-Ho;Kim, Chang-Gyu;Jeong, Gil-Saeng;Kim, Jong-Bae
    • Korean Journal of Animal Reproduction
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    • v.15 no.1
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    • pp.67-77
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    • 1991
  • 本 試驗은 受精初期段階에서 생쥐卵子 透明帶의 機能을 糾明하고 種特異的인 精子受容體의 存在 및 構造理解를 위한 基礎硏究로서 ICR 생쥐의 透明帶를 免疫原으로 하여 토끼에서 抗透明帶抗體를 生産하고 免疫分析法 및 生物學的 檢査法으로 生産된 抗體가 생쥐 透明帶에 特異性이 있음을 確認한 후 이 抗體가생쥐 未受精卵의 體外受精에 미치는 影響과 또 이 抗體를 생쥐에 受動免疫하였을 때 受精에 미치는 影響을 調査하였던 바 다음과 같은 結果를 얻었다. 1. 處理區로서 抗血淸을, 對照區로서 對照血淸을 卵子培養液에 各各 0.3~10%로 含有한 培養液에서 생쥐의 未受精卵을 2時間 培養한 後 觀察하였을 때 對照區 卵子들의 透明帶가 正常的인 形態를 나타내었던 反面에, 處理區의 卵子들은 모두 透明帶 表面에 뚜렷한 沈澱層을 나타내었다. 이어 對照區와 處理區의 卵子를 0.1% pronase가 含有된 培養液으로 옮긴 후 觀察하였을 때, 對照區 卵子들의 透明帶가 5~7分만에 完全히 融解한 反面에, 處理區에서는 2~3時間까지 殘存하였다. 2. 處理區와 對照區 卵子를 FITC-goat anti-rabbit IgG가 含有된 培養液에서 養液한 후 螢光顯微鏡下에서 觀察하였을 때 對照區에서 對照血淸을 10% 含有한 培養液에서 培養한 일부 卵子들의 透明帶에서 희미한 螢光이 나타났으나 血淸稀釋度가 增加함에 따라 전혀 螢光을 나타내지 않았던 反面에, 處理區에서는 抗血淸이 1 : 1,000으로 含有된 培養液에서 培養한 卵子의 透明帶에서도 강한 螢光이 나타났다. 3. 帶抗體를 抗原으로 하여 間接酵素免疫分析法을 實施하였을 때 抗血淸과 對照血淸이 PBS에 1 : 200까지 稀釋되었을 때의 O.D.價는 별다른 差異를 나타내지 않았으나 血淸의 稀釋度가 增加함에 따라 O.D.價가 有意한 差異를 나타냄으로써 透明帶에 特異性을 갖는 抗體임을 確認할 수 있었다. 4. 抗血淸과 對照血淸이 各各 0.3~10%까지 含有된 培養液으로 생쥐의 未受精卵을 豫備培養한 다음 體外受精을 實施하였을 때의 受精率은 對照區에서 77.7%~87.7%이었던 反面에, 處理區에서는 0~29.8%로서 抗透明帶抗體가 생쥐卵子의 體外受精을 完全히 또는 顯著히 抑制하였다. 그러나 抗血淸과 對照血淸이 各各 2.5~10%까지 含有된 培養液으로 透明帶를 除去한 未受精卵의 體外受精을 하였을 때의 受精率은 處理區와 對照區에서 各各 93.0~98.3% 및 93.3~94.3%로서 受精抑制現象을 나타내지 않았다. 5. 抗血淸과 正常血淸, 그리고 兩 血淸으로부터 protein A-affinity chromatography에 의해 分離한 immunoglubulin G를 各各 생쥐에 受動免疫하였을 때, 對照區로서 對照血淸을 0.1~0.3ml 또는 control IgG를 1~3ml 投與했을 때의 各各의 受精率이 80.4~90.2%, 82.2~94.4%이었던 反面에, 處理區로서 抗血淸을 0.3ml 또는 immune IgG를 3mg 投與한 생쥐에서는 完全한 受精抑制現象이 確認되었다.

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A Study on Wasteform Properties of Spent Salt Treated with Zeolite and SAP (염화염을 제올라이트와 SAP로 처리한 고화체의 특성연구)

  • Kim, Hwan-Young;Park, Hwan-Seo;Kang, Kweon-Ho;Ahn, Byung-Gil;Kim, In-Tae
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.8 no.2
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    • pp.99-105
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    • 2010
  • This paper investigated the characteristics of wasteform containing a spent zeolite used as a separating agent of FPs for recycling LiCl waste which would be generated from pyrochemical process of spent PWR fuel. In this study, a conventional borosilicate and Ca-rich glass were used as a consolidating agent for spent zeolite and it's mixing ratio was changed in the range, $25{\sim}35wt%$. The leach rates of Cs and Sr had about $0.1{\sim}0.01g/m^2day$ and $0.001{\sim}0.0001g/m^2day$, respectively. The leach resistance of Cs increased with amount of SAP and it showed about 10 times higher in the Ca-rich glass wasteform than in the conventional borosilciate glass wasteform. The compressive strength of wasteform was affected with the amount of glass. Thermal expansion rate of containing 30wt% glass has relatively lower than others. Also, the melting temperature was little changed in given mixing ratio of glass.

A Study on the Reduction of Non-Point Source Pollution loads from Small Agricultural Watershed by Applying Surface Covering Scenario using HSPF Model (HSPF 모델을 이용한 지표피복 시나리오 적용에 따른 농촌 소유역에서의 비점원오염 저감연구)

  • Jung, Chung-Gil;Park, Jong-Yoon;Kim, Sang-Ho;Kim, Seong-Joon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.103-103
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    • 2012
  • 본 연구에서는 시험포장($1276.6m^2$)에서의 지표피복 BMPs (Best Management Practices) 시나리오를 적용하여 얻은 평균 유출저감율을 HSPF 모델에 적용하여 유역차원에서의 비점원오염 저감효과를 평가하고자 한다. 본 연구에서는 별미천 유역($1.21km^2$)을 대상으로 모형의 적용을 위한 입력자료로 기상자료와 지형자료를 구축하였으며 기상자료로 수원, 양평, 이천 기상관측소 자료를 구축하였으며, 지형자료로 격자크기 2m의 DEM (Digital Elevation Model)과 토지이용도는 2006년 5월 1일 QuickBird 영상을 제공받아 기존 환경부, 건교부, USGS의 토지피복분류체계 및 현장조사를 통하여 QuickBird 영상으로부터 추출 가능한 정밀농업정보에 대한 항목을 결정하였으며, 정사보정된 QuickBird 영상을 스크린 디지타이징 기법(On-Screen Digitizing Method)을 이용하여 총 21개 토지이용항목의 정밀토지이용도를 구축하였다. 실제모니터링으로 측정된 자료를 바탕으로 수위-유량곡선 산정 및 오염부하곡선을 선정, 2011년 6월 8일부터 10월 31일 분석기간으로 HSPF 모델링을 실시하였으며 모의결과 월별 통계에 따른 적용성 분석으로 RMSE (Root Mean Square Error) 는 1.15 ~ 1.76(mm/day), $R^2$는 0.62 ~ 0.78, Nash-Sutcliffe model efficiency (NSE)는 0.62 ~ 0.76로 모의치는 실측치와 유의성이 있는 것으로 분석되었다. 또한, Sediment, T-N, T-P의 $R^2$는 각각 0.72, 0.62, 0.63으로 상관성을 보이는 것으로 분석되었다. 시험포장으로부터 얻어진 event별 볏짚을 이용한 지표피복시나리오적용 후 밭에서의 평균 유출 약 10 % 유출율 감소 조건과 실제 평균 비점원오염 저감효과 89.7 % ~ 99.4 %의 결과로부터 지표피복효과의 침투효과를 HSPF 모델로 적용하기 위해 침투량(INFILT)를 조절하여 평균유출 약 10 %가 감소되는 16.0 mm/hr 값을 선정하였다. 그 결과, Sediment. T-N, T-P의 평균 저감율은 각각 87.2 %, 28.5 %, 85.1 %로 나타났으며 이는 시험포장에서의 실제 평균 비점오염 저감효과 89.7 % ~ 99.4 %에 근접함을 알 수 있었다. 이 결과로부터 침투량 조절에 따른 지표피복(침투짚단)효과는 Sediment, T-P에서 저감효율이 80 % 이상으로 높았지만 T-N은 약 30 %로 낮은 저감율을 보임으로써 저감효과가 크지 않음을 나타냈다.

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Behavior of Implanted Dopants and Formation of Molybdenum Siliclde by Composite Sputtering (Composite target으로 증착된 Mo-silicide의 형성 및 불순물의 거동)

  • Cho, Hyun-Choon;Paek, Su-Hyon;Choi, Jin-Seog;Hwang, Yu-Sang;Kim, Ho-Suk;Kim, Dong-Won;Shim, Tae-Earn;Jung, Jae-Kyoung;Lee, Jong-Gil
    • Korean Journal of Materials Research
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    • v.2 no.5
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    • pp.375-382
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    • 1992
  • Molybdenum silicide films have been prepared by sputtering from a single composite MoS$i_2$ source on both P, B$F_2$respectively implanted (5${\times}10^{15}ions/cm^2$ single crystal and P implanted (5${\times}10^{15}ions/cm^2$) polycrystalline silicon substrates followed by rapid thermal annealing in the ambient of argon. The heat treatment temperatures have been varied in the range of 600-l20$0^{\circ}C$ for 20 seconds. The properties of Mo-silicide and the diffusion behaviors of dopant after the heat treatment are investigated using X-ray diffraction, scanning electron microscopy(SEM) , secondary ions mass spectrometry(SIMS), four-point probe and $\alpha-step.$ Annealing at 80$0^{\circ}C$ or higher resulted in conversion of the amorphous phase into predominantly MoS$i_2$and a lower sheet resistance. There was no significant out-diffusion of dopants from both single crystal and polycrystalline silicon substrate into molybdenum silicide layers during annealing.

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Detection of Human Papillomavirus and Expression of MHC Class I in Laryngeal Squamous Cell Carcinoma (후두편평세포암종에서 Human papillomavirus의 검출과 주조직적합복합체(Major Histocompatibility Complex: MHC) Class I 발현양상)

  • Oh, Byung-Kwon;Hwang, Chan-Seung;Hong, Young-Ho;Kim, Hoon;Kim, Chun-Gil;Min, Hun-Ki
    • Korean Journal of Bronchoesophagology
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    • v.3 no.1
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    • pp.70-78
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    • 1997
  • The development of preneoplastic and neoplastic squamous cell proliferations of body sites such as the skin, female lower genital tract, and larynx is strongly associated with specific types of human papillomaviruses (HPV). Antitumor $CD^{8+}$ cells recognize peptide antigens presented on the surface of tumor cells by major histocompatibility complex (MHC) class I molecules. The MHC class I molecule is a heterodimer composed of an integral membrane glycoprotein designated the alpha chain and a noncovalently associated, soluble protein called beta-2-microglobulin( $\beta$ -2-m). Loss of $\beta$-2-m generally eliminates antigen recognition by antitumor $CD^{8+}$ T cells. We evaluated the expression of $\beta$-2-m as a potential means of tumor escape from immune recognition and the presence of HPV DNA as a cause of laryngeal squamous cell carcinomas (SCCs). Laryngeal SCCs (n=39) were analyzed for MHC class I expression by immunohistochemistry and for presence of HPV by in situ hybridization technique. The results were as follows : 1) HPV DNA was detected in 10 (25.64%) out of 39 cases in laryngeal squamous cell carcinomas. 2) MHC class I down-regulation (heterogenous and negative expression) in HPV positive lesions was higher than HPV negative lesions. 3) The expression of MHC class I was related to cellular differentiation regardless of T-stage and nodal involvement. In conclusion, HPV was thought to be the etiological factor of SCC of larynx, and we found that the down-regulation of MHC class I was a common phenomenon In laryngeal SCC and may provide a way for tumor cells to escape from immune surveillance.

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The Effect of Acetonitrile on the Texture Properties of Sodium Silicate Based Silica Aerogels (아세토니트릴 첨가가 물유리 기반 실리카 에어로겔의 기공구조에 미치는 영향)

  • Kim, Younghun;Kim, Taehee;Shim, Jong Gil;Park, Hyung-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.4
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    • pp.143-148
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    • 2018
  • Sodium silicate based silica aerogels are lower in cost than silica alkoxide based silica aerogels, but the demand is decreasing as their physical properties are lowered. In this research, acetonitrile as a drying control chemical additive (DCCA) is added in the sol state to improve the pore-structural properties of sodium silicate based silica aerogel by preventing the agglomeration of particles and cross-linked bond. The sodium silicate based silica aerogel by ambient pressure drying were prepared by sol-gel process. Acetonitrile/$Na_2SiO_3$ molar ratio of 0, 0.05, 0.1, 0.15, and 0.2 was added to the sol state. The physical properties of the final product were analyzed using Fourier transform infrared, contact angle measurement, Brunauer-Emmett-Teller and Barrett-Joyner-Halenda measurements and field emission scanning electron microscopy. It was confirmed that the sample with adding 0.15 molar ratio of acetonitrile and sodium silicate showed a high specific surface area ($577m^2/g$), a high pore volume (3.29 cc/g), and a high porosity (93%) comparable to the pore-structural properties of silica alkoxide based silica aerogels.

Effects of Cervical Stabilization and Scapular Stabilization Exercise on the Proprioception and Craniovertebral Angle and Upper Trapezius Muscle Tone of People with F orward Head Posture

  • Seung-Hwan, Lee;Byoung-Ha, Yoo;Hyun-Seo, Pyo;Dongyeop, Lee;Ji-Heon, Hong;Jae-Ho, Yu;Jin-Seop, Kim;Seong-Gil, Kim
    • Journal of the Korean Society of Physical Medicine
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    • v.17 no.4
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    • pp.1-13
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    • 2022
  • PURPOSE: This study examined the effects of the craniovertebral angle, proprioception (joint error test), and the upper trapezius on the muscle tone when comparing cervical stabilization and scapula stabilization exercises and when two exercises were performed together. METHODS: The participants in this study agreed in advance, and this study was carried out by recruiting 27 university students in their twenties with mild frontal posture. The subjects were assigned randomly to three groups that performed cervical stabilization exercises, scapular stabilization exercises, and both cervical and scapular stabilization exercises. One-way repeated ANOVA was used to analyze the evaluation values of the 1st, 3rd, and 6th weeks of exercise intervention within the group, and one-way ANOVA was used to compare the difference in the effects of exercise intervention among the three groups. RESULTS: Proprioception was significantly different in the cervical stabilization exercises (CSE) group and the cervical stabilization exercises + Scapular stabilization exercises (CSE+SSE) groups at three weeks, and there was a significant difference between the scapular stabilization exercises (SSE) group and the CSE+SSE group (p < .05). At six weeks, there was a significant difference between the CSE group and the CSE+SSE group, and there was a significant difference between the SSE group and the CSE+SSE group (p < .05). There was a significant difference between three and six weeks in the CSE group (p < .05). In the SSE group, there was a significant difference between pre and six weeks, and between three and six weeks (p < .05). In the CSE+SSE group, there was a significant difference between pre and three weeks, and between pre and six weeks (p < .05). On the other hand, there were no significant differences between CVA and muscular tone in all three groups (P > .05). CONCLUSION: In all groups, the proprioception (joint error test) showed significant improvement, and the CSE+SSE group showed greater improvement than the other groups. As a result, the appropriate combination of neck stabilization exercise and scapular stabilization exercise effectively improved proprioception in the presence of forward head posture (FHP).