• Biotechnology and Bioprocess Engineering:BBE
• /
• v.11 no.3
• /
• pp.235-239
• /
• 2006

Due to recent advances in genome sequencing, there has been a dramatic increase in the quantity of genetic information, which has lead to an even greater demand for a faster, more parallel expression system. Therefore, interest in cell-free protein synthesis, as an alternative method for high-throughput gene expression, has been revived. In contrast to in vivo gene expression methods, cell-free protein synthesis provides a completely open system for direct access to the reaction conditions. We have developed an efficient cell-free protein synthesis system by optimizing the energy source and S30 extract. Under the optimized conditions, approximately $650{\mu}g/mL$ of protein was produced after 2h of incubation, with the developed system further modified for the efficient expression of PCR-amplified DNA. When the concentrations of DNA, magnesium, and amino acids were optimized for the production of PCR-based cell-free protein synthesis, the protein yield was comparable to that from the plasmid template.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.15 no.5
• /
• pp.490-496
• /
• 2015

We have grown AlN/GaN heterostructure which is a promising candidate for mm-wave applications. For the growth of the high quality very thin AlN barrier, indium was introduced as a surfactant at the growth temperature varied from 750 to $1070^{\circ}C$, which results in improving electrical properties of two-dimensional electron gas (2DEG). The heterostructure with barrier thickness of 7 nm grown at of $800^{\circ}C$ exhibited best Hall measurement results; such as sheet resistance of $215{\Omega}/{\Box}$electron mobility of $1430cm^2/V{\cdot}s$, and two-dimensional electron gas (2DEG) density of $2.04{\times}10^{13}/cm^2$. The high electron mobility transistor (HEMT) was fabricated on the grown heterostructure. The device with gate length of $0.2{\mu}m$ exhibited excellent DC and RF performances; such as maximum drain current of 937 mA/mm, maximum transconductance of 269 mS/mm, current gain cut-off frequency of 40 GHz, and maximum oscillation frequency of 80 GHz.

• Journal of Korean Elementary Science Education
• /
• v.33 no.4
• /
• pp.762-772
• /
• 2014

The purpose of this study was to investigate the effects of science lesson applying STEAM education on the creative thinking activities and emotional intelligence of elementary school students. The study subjects were two classes of the $3^{th}$ grade of S elementary school in B Metropolitan City. One class including 26 students was experimental group and the other including 27 students was comparison group. For the purpose of study, the lesson unit 'The world of animals' was practised, the reorganized unit applying STEAM was applied to experimental group, whereas comparison group was taught traditional science lesson. The results of this study were as follows. First, the science lesson applying STEAM education influenced significantly the improvement of the creative thought activities of elementary school students. Second, the science lesson applying STEAM education influenced significantly the improvement of the emotional intelligence of elementary school students.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2005.11a
• /
• pp.73-76
• /
• 2005

한국방송(KBS)은 전자통신연구원(ETRI)과 공동으로 송수신 안테나의 낮은 분리도(isolation)하에서도 운용이 용이하며 중계기 내부의 왜곡을 보상할 수 있는 DOCR(Digital On-Channel Repeater)을 개발하였다. 개발된 DOCR의 성능과 서비스 커버리지를 측정하기 위하여 수원 지역에서 2004년과 2005년 두 차례의 필드테스트를 공동으로 실시하였다. 본 논문에서는 개발된 DOCR시스템에 대하여 그 특징을 설명하고 수원 지역에서 실시된 필드테스트 결과를 분석한다. 또한, 다양한 세대의 ATSC 수신기를 측정에 사용함으로써 수신기 성능에 따른 DOCR 서비스 커버리지의 변화를 측정하였다. 측정결과에는 DOCR의 사용에 따른 수신성공률, 수신마진, 수신용이성(수신가능각도)등이 포함되며 측정 지역의 수 년간의 테스트 결과를 종합하여 서비스 범위내의 수신성능을 분석한다.

• Nuclear Engineering and Technology
• /
• v.41 no.4
• /
• pp.455-476
• /
• 2009

The history of nuclear fusion research in Korea is rather short compared to that of advanced countries. However, since the mid-1990s, at which time the construction of KSTAR was about to commence, fusion research in Korea has been actively carried out in a wide range of areas, from basic plasma physics to fusion reactor design. The flourishing of fusion research partly owes to the fact that industrial technologies in Korea including those related to the nuclear field have been fully matured, with their quality being highly ranked in the world. Successive pivotal programs such as KSTAR and ITER have provided diverse opportunities to address new scientific and technological problems in fusion as well as to draw young researchers into related fields. The frame of the Korean nuclear fusion program is now changing from a small laboratory scale to a large national agenda. Coordinated strategies from different views and a holistic approach are necessary in order to achieve optimal efficiency and effectiveness. Upon this background, the present paper reflects upon the road taken to arrive at this point and looks ahead at the coming future in nuclear fusion research activities in Korea.

• Proceedings of the KIEE Conference
• /
• 1994.07b
• /
• pp.1536-1539
• /
• 1994

The influence due to metallic particle contaminated on spacer surface is remarkable in the decreasing of dielectric strength in $SF_6$ GIS. In relation with this problem, We studied, AC flash-over voltage characteristics and breakdown mechanism are investigated under metallic particle initiated condition in $SF_6$ gas by varying the particle position, particle shape with a plane-plane electrode. The main results arc as follows 1. The small amount of the metallic particle in the gap do not make flash-over voltage to be influence, but the significant decrease of th flash-overed voltage is result in case of the big and long size of the metallic paraticle. 2. Influence of the flash-over voltage are lowest in the mid and are highest in the electrode of metallic particle position. 3. In case of the initiated metallie particle, The more the pressure are high, the more the recluced ratio of flash-over voltage are high. 4. The metallic particle shape which results in the reduced flash-over voltage forced the critical pressure to move in to the region of low pressure. 5. The existance of the metallic particle on the upper electrode side and high pressure make the decreasing ratio of flash-over voltage bigger than that of the ground side electrode.

• Proceedings of the KIEE Conference
• /
• 2000.07c
• /
• pp.1518-1520
• /
• 2000

Amorphous $MnO_{2}{\cdot}nH_{2}O$ in 1M KOH aqueous electrolyte proves to be an excellent electrode for a faradic electrochemical capacitor cycled between -0.5 and +1.0 versus Ag/AgCl. The effect of thermal treatment on the crystalinity, particle structure, and corresponding electrochemical properties of the resulting xerogel remained amorphous as Mn(OH)2 up to 160$^{\circ}C$. With an increase in the temperature above 200$^{\circ}C$, both the surface area and pore volume decreased sharply, because the amorphous Mn(OH)2 decomposed to form MnO that was subsequently oxidized to form crystalline Mn3O4. In addition, the changes in the crystallinity, and particle structure all had significant but coupled effects on the electrochemical properties of the xerogels. A maximum capacitance of 160.6F/g was obtained for an electrode prepared with the MnOx Xerogel calcined at 150$^{\circ}C$, which was consistent with the maxima exhibited in both the surface area and pore volume. This capacitance was attributed solely to a surface redox mechanism.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.36 no.3B
• /
• pp.220-230
• /
• 2011

Mobile RFID which integrates mobile network with RFID technique is the technique to get the information of products by transmitting the identifier through mobile network after reading the identifier of RFID tag. It attached on the equipment as the mobile phone. However, mobile RFID has the privacy-related problem among requested secure problems required from the existing RFID. In this paper, the random key created by mobile RFID reader and Tag during the inter-certificating and initialization procedure for mobile RFID users to receive tag information from backend server securely is divided into random sizes and any information on the tag which requires the protection of privacy shouldn't be provided to anyone. In performance analysis, previous protocol used modulo operation in registration and login computation. But the proposed protocol has higher efficiency in saving space and computation volume than MARP scheme and Kim. et. al scheme because our protocol is accomplished by mutual authentication and registering user information through one-way hash function.

• Korean Journal of Oriental Medicine
• /
• v.17 no.3
• /
• pp.97-104
• /
• 2011

Objectives : In this paper, we proposed a method for selecting reliable variables from various vocal features such as frequency derivative features, frequency band ratios, intensities of 5 vowels and an intensity of a sentence, since some features are sensitive to the variation of a subject's utterance. Methods : To obtain the reliable voice variables, the coefficient of variation (CV) was used as the index to evaluate the level of reliability. Since the distributions of a few features are not Gaussian, but are instead skewed to the right or left, we transformed the features by taking the log or square root. Moreover, the definition of the variables that are suitable to represent the vocal property was explained and analyzed. Results : At first, we recorded the vowels and the sentence five times both in the morning and afternoon of the same day, totally ten recordings from each of six subjects (three males and three females). We then analyzed the CVs of each subject's voice to obtain the stable features with a sufficient repeatability. The features having less than 20% CVs for all six subjects were selected. As a result, 92 stable variables from the 222 features were extracted, which included all the transformed variables. Conclusions : Voice can be widely used to classify the four constitution types and to recognize one's health condition from extracting meaningful features as physical quantity in traditional Korean medicine or Western medicine. Therefore, stable voice variables can be useful in the u-Healthcare system of personalized medicine and for improving diagnostic accuracy.

• Analytical Science and Technology
• /
• v.13 no.1
• /
• pp.108-120
• /
• 2000

To perform an effective screening for toxic materials of forensic interest detected in high profile criminal case in biological and environmental samples, we tried to construct a searchable computerized database using HPTLC(High Performance Thin Layer Chromatography) and GC/MS. Retardation factor($R_f$) values and UV spectral data of HPTLC were investigated for 160 pesticides, 34 chemicals and 39 explosives of standard grade. The data were compiled in a library. We also analyzed 112 pesticides, 31 chemicals and 17 explosives and 57 volatile organic compounds(VOCs) by GC/MS. The data for RT and characteristic mass ions were also compiled in a library.