• IMMUNE NETWORK
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• 제23권1호
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• pp.2.1-2.19
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• 2023

Immune diversification helps protect the host against a myriad of pathogens. CD8⁺ T cells are essential adaptive immune cells that inhibit the spread of pathogens by inducing apoptosis in infected host cells, ultimately ensuring complete elimination of infectious pathogens and suppressing disease development. Accordingly, numerous studies have been conducted to elucidate the mechanisms underlying CD8⁺ T cell activation, proliferation, and differentiation into effector and memory cells, and to identify various intrinsic and extrinsic factors regulating these processes. The current knowledge accumulated through these studies has led to a huge breakthrough in understanding the existence of heterogeneity in CD8⁺ T cell populations during immune response and the principles underlying this heterogeneity. As the heterogeneity in effector/memory phases has been extensively reviewed elsewhere, in the current review, we focus on CD8⁺ T cells in a "naive" state, introducing recent studies dealing with the heterogeneity of naive CD8⁺ T cells and discussing the factors that contribute to such heterogeneity. We also discuss how this heterogeneity contributes to establishing the immense complexity of antigen-specific CD8⁺ T cell response.

• Clinical Endoscopy
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• 제55권5호
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• pp.581-587
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• 2022

Procedural sedation has become increasingly common in endoscopy. Sedatives and analgesics induce anxiolysis and amnesia. In addition, an appropriate level of sedation is necessary for safe procedures including therapeutic endoscopy. Midazolam and propofol are the most commonly used drugs in sedative endoscopy. In recent years, the need to ascertain the safety and effectiveness of sedation has increased in practice. Therefore, new sedatives and analgesic drugs for optimal sedative endoscopy, have recently emerged. This article reviews the characteristics of sedatives and analgesics, and describes their clinical use in gastrointestinal endoscopy.

• 수산해양기술연구
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• 제41권1호
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• pp.46-53
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• 2005

통영 및 장승포 지역에 설치된 인공어초의 침하특성을 조사하기 위해서 중력시추기를 이용한 해저 퇴적물 채취와 탄성파 탐사기 및 측면 주사 음향 탐지기를 이용한 인공어초의 상태를 조사하였다. 퇴적물 시료는 실험실에서 입도 및 전단강도에 대한 측정이 이루어 졌고 탄성파 자료는 음향 특성에 따라 그 특징을 해석하였다. 탄성파 자료를 분석한 결과 연구지역에 광범위하게 분포하고 있는 퇴적층에 인공어초가 어느 정도 침하되어 있음을 알 수 있었다. 또한 측면 주사 음향 탐지기의 영상을 분석한 결과 대부분의 인공어초들은 군집 혹은 서로 중첩되어 분포하고 있는 것으로 나타나서 음향자료를 이용한 인공어초의 상태를 전반적으로 파악할 수 있었다. 획득된 주상시료 분석 결과 평균 입도 5${\sim}$9${\Phi}$이며, 전단강도는 1${\sim}$5kPa를 보였다. 평균 입도의 경우 정점별로 수직적인 값의 변화는 작은 반면, 전단강도 값은 평균 입도에 비해 깊이가 깊어질수록 증가하는 양상을 보였다. 특히, 전단강도 값은 퇴적층 깊이 약 20${\sim}$40cm에서 갑자기 증가하는데 이것은 조사 지역에서 실시한 잠수조사 결과를 바탕으로 인공어초의 침하깊이 20${\sim}$30cm와 거의 일치하는 것으로 나타났다. 따라서 조사 지역의 경우 전단강도 값이 약 2${\sim}$3kPa 이상을 가지는 곳에서는 침하 현상이 거의 일어나지 않거나 아주 느리게 일어나고 있는 것으로 사료된다. 본 조사 지역과 같이 니질의 함량이 높고 입도가 유사한 경우에도 퇴적 과정 혹은 퇴적물 특성의 차이나 퇴적 후의 속성 작용에 의한 전단강도 값의 증가를 기대할 수 있으며, 또한 인공어초의 종류와 크기, 무게, 형태 등에 따른 해저면의 침하 특성도 다를 수 있기 때문에 향후 보다 종합적인 요인에 대한 분석이 이루어진다면 연약 지반 해역에 대한 인공어초의 시설 대상 범위는 지금보다 더 확대될 것으로 판단된다.

• Journal of Digestive Cancer Research
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• 제5권2호
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• pp.86-90
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• 2017

Background: The aim of this study was to investigate clinicopathologic features of type 3 gastric neuroendocrine neoplasm (NEN) by treatment modality. Methods: The Korean Society of Gastrointestinal Cancer conducted the Korean Gastroenteropancreatic Neuroendocrine Tumor Registry, a retrospective registry database of gastroenteropancreatic neuroendocrine tumors from 16 hospitals in Korea. The normal serum gastrin level range was defined as <100 pg/mL, and gastric NEN patients with normal gastrin level were selected for analysis. Results: Among 358 patients with gastric NEN, 21 (5.9%) patients were classified with type 3 gastric NEN. The median age was 53 years (range 30-74). According to the WHO 2010 classification, 13 (61.9%) patients had grade 1, and 8 (38.1%) patients had grade 2 or 3. Endoscopic treatment was performed in 14 (66.7%) patients, and surgery was performed in 7 (33.3%) patients. The tumor size was smaller in the endoscopic treatment group than in the surgery group (0.6 cm vs 1.3 cm, p=0.006). After treatment, there was one recurrence in the surgery group. Conclusion: In small size Type 3 gastric NEN, endoscopic treatment was associated with a good prognosis, compared to surgery. Thus, endoscopic treatment can be used an alternative modality in selected cases of type 3 gastric NEN.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.226-226
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• 2017

This study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different covering material on stable winter survival management with edible leaf in Ramie(Boehmeria nivea L.). The method of winter survival with covering material were conducted under three condition compose to Non covering, Rice straw cutting covered with 500kg.10a-1, Rice husks covered with 1,000kg.10a-1(covered 4~5 cm thickness in the soil surface). Method of application were standard application(N-P-K-Compost applied at 27-9-27-600kg.10a-1. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. 20% of top dressing were four times application in March 25 - October 5. Planting year were March 15, 2011. Plants were spaced 60 cm apart in rows 25 cm apart with open cultivation. According to non covering < Rice husks covered with 1,000kg.10a-1 < Rice straw cutting covered with 500kg.10a-1 cultivation this order, aerial part as a result were plenty amount of growth. Sprout time and winter survival rates was uncovering control plot compared to 2 - 5 days quickly, 45-57% highly by rice husks and rice straw covering. Green leaf yields is untreated control plot (12,44 kg.10a-1) compared to rice husks covering 7% higher, and rice straw covering increased to 18% of the most.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.181-188
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• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제23권2호
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• pp.185-190
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• 2001

We described three cases of immediate reimplantation of a frozen-thawed autogenous mandible composed of a mixture of iliac bone, marrow, and particulate hydroxyapatite in tumors of the mandible. Acceptable outcomes were obtained in three patients who underwent immediate autogenous mandibular graft reconstruction. The conditions leading to successful outcome of the procedure are also discussed. Reimplantation of frozen autogenous lesioned mandible was performed in three patients with mandibular tumors. Two reimplanted grafts survived without complications following surgery. One case had postoperative infection that resolved with appropriate antibiotic treatment. There were no recurrences of the primary lesions. Satisfactory facial contour after surgery was achieved. These results are most promising, and we believe that, with further refinement, this technique will offer a new and acceptable modality for facial reconstruction in patients with cancer.

• International Journal of Industrial Entomology and Biomaterials
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• 제37권2호
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• pp.95-99
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• 2018

In insect defense system, antimicrobial peptides (AMPs) are one of important biological molecules to survive in a variety of environments. Insect can synthesize AMPs to protect against invading pathogens in humoral immune response. Taking more advantage of biological antimicrobial molecules, we report antibacterial activity of two cecropin type peptides, cecropin and moricin, isolated from the silkworm against four salmonella species. In this work, we purified antimicrobial candidate peptides (AMCP) from the extracts of immune challenged silkworm larval hemolymph by two-step chromatographic purification procedure, cation exchange and gel permeation chromatography. The molecular weights of purified peptides were estimated to be about 4 ~ 5 kDa by Tricin SDS-PAGE analysis, and identified as silkworm cecropin and moricin by NCBI BLAST homology search with their N-terminal amino acid sequences. As antibacterial activity assay, the purified peptides showed stronger antibacterial activity against Salmonella pathogens with an MIC value of $1{\sim}4{\mu}g/mL$. Therefore two cecropin type peptides purified from the silkworm will be valuable potential materials for development of new natural antibiotics.

• International Journal of Industrial Entomology and Biomaterials
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• 제39권1호
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• pp.14-21
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• 2019

Genome editing by CRISPR/Cas9, a third-generation gene scissor in molecular breeding at the genome level, is attracting much attention as one of the breeding techniques of the future. In this study, genetic and phenotypic analysis was used to examine the responsiveness of the Bakokjam variety of the silkworm Bombyx mori to molecular breeding using CRISPR/Cas9 in editing the white egg 2 (w-2) gene. The nucleotide sequence of the w-2 gene was analyzed and three different guide RNAs (gRNA) were prepared. The synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the Bm-N silkworm cell line. To edit the silkworm gene, W1N and W2P gRNA and Cas9 complexes were microinjected into silkworm embryos. Based on the results of microinjection, the hatching rate was 16-24% and the incidence of mutation was 33-37%. The gene mutation was verified in the heterozygous F1 generation, but no phenotypic change was observed. In F2 homozygotes generated by F1 self-crosses, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and will be a very effective way to shorten the time required than the traditional breeding process.

• Archives of Pharmacal Research
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• 제19권4호
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• pp.321-325
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• 1996

Aiming at the development of anticancer agents by modification of phenolic benzo[c]phenanthridine alkaloid, additional hydroxyl group was put on C10 position of fagaridine (1) by a biomimetic synthetic procedure to afford 10-hydroxyfagaridine (12). All of the synthetic intermediates were also screened in vitro antitumor activities against five different cell lines as well as 12. Among them the representative cytotoxic results are shown as follows; P-quinone (11) $[ED_50;(A549=0.22; {\mu}g/ml)$, $(HCT;15=0.21 {\mu}g/ml)$, fagaridine (1) $(HCT;15=0.41 {\mu}g/ml)$, olefin (6) $(HCT; 15=0.06 {\mu}g/ml)$, acetal (7) $(SKMEL-2=0.07 {\mu}g/ml)$, dihydrofagaridne (10) $(A549=0.38 {\mu}g/ml)$, 10-hydroxyfagaridine (12) $(A 549=0.45{\mu}g/mi)$. From these observation three main remarks can be drawn; (i) the iminium part of benzo[c]phenanthridine is not essential for showing acitvities, (ii) the additional hydroxyl group did not contribute to enhance the cytotoxicity, (iii) the 3-arylisoquinolin-1(2H)-one derivatives were found to display significant in vitro antitumor activity.