• 제목/요약/키워드: Gibberella zeae

검색결과 32건 처리시간 0.018초

한국에 발생하는 맥류의 적미병균의 동정 (Identification of the Causal Organism of Cereal Scab in Korea)

  • 이배함;라민근;최태주
    • 미생물학회지
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    • 제2권1호
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    • pp.25-27
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    • 1964

  • Lee, Bae Ham, Rha, Min Keun and Choi, Tae joo (Dept. of Biology, Kon Kuk University). Identification of the causal organism of cereal scab in Korea. Kor. J. Microbiol. Vol. 2, No. 1, p. 25-27 (1964) Head blight or scab occurred in barley, wheat, rye and some other cereals widely in this country during the spring of 1963. The causal organisms were collected from 34local areas and isolated purely. All isolates identified as Gibberella zeae (Schw.) Petch. and Fusarium graminearum Schw. as conidial stage.

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원형 곤포사일리지에 발생한 곰팡이의 분류 동정 (Taxonomy and Identification of Fungi Isolated from Round Bale Silage)

  • 노환국;여준모;김완영;이장형;서성;김민경;서건식
    • 현장농수산연구지
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    • 제14권1호
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    • pp.61-83
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    • 2012

  • 곤포사일리지에 발생하는 곰팡이를 분류 동정하기 위하여 2009년부터 2011년까지 253개의 오염된 곤포사일리지를 채집하였다. 이탈리안라이그라스 수단 그라스, 호밀, 옥수수, 보리와 귀리에서 분리한 총 253 목초 샘플을 분석했다. 채집된 곤포사일리지에서 총 270균주를 순수 분리하였고, 분리된 곰팡이의 형태와 rDNA sequence를 분석하여 분류 동정하였다. 분리한 곰팡이를 형태를 기준으로 분류 동정 한 결과 Rhizopus sp., Fusarium spp., Coprinus sp., Blastomyces sp., Aureobasidium sp., Polypaecilum sp., Botryoderma sp., Mucor sp., Scytalidium sp., Sphaeropsis sp., Aspergillus spp., Trichocladium sp., Humicola sp., Staphylotrichum sp., Periconia sp., Verticillium sp., Diplococcium sp., Penicillium spp., Trichoderma spp. 등 19종의 곰팡이가 분리 동정되었다. 한편, 사일리지에서 분리한 곰팡이의 염기서열을 분석한 결과 Acremonium strictum, Asperillus tubingensis, Bionectria ochroleuca Dipodascaceae sp., Fusarium proliferatum, Fusarium oxysporum, Fusrium solani, Gelasinospora reticulata, Gibberella monliliformis, Gibberella zeae, Nectria mauritiicola, Penicillium paneum, Pseudallecheria boydii, Schizaphyllum commune, Scoplariopsis brevicaulis, SimpliciIlium lamellicola로 분류 동정되었다. PenicIlliwn sp.와 Trichoderma sp.는 각각 74와 64균주가 분리되어 가장 많이 분리 동정되었고, Humicola sp., Aspergillus sp., Coprinus sp., Fusarium spp.도 10~30 균주가 분리 동정되었다. 곤포 사일리지에서의 대부분의 곰팡이는 한 종 이상의 곰팡이가 복합적으로 오염되었다.

  • https://doi.org/10.23097/JPAF:2012.14(1).61 인용 PDF

Shifting reproductive mode of a mycotoxin producing-fungus by manipulation of mating-type genes

  • Lee, Jungkwan;Lee, Teresa;Lee, Yin-Won;Yun, Sung-Hwan;Gillian Turgeon
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.85.1-85
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    • 2003

  • In most ascomycetes, a single mating type locus, MAT, with two alternate forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes these alternate idiomorphs reside in different nuclei. In contrast, most homothallic ascomycetes carry both MAT1-1 and MAT1-2 in a single nucleus, usually closely linked. An example of the latter is Gibberella zeae, a producer of mycotoxins such as trichothecene and zearalenone that threaten human and animal health. We asked if G. zeae could be made strictly heterothallic by manipulation of MAT. Targeted gene replacement was used to differentially delete MAT1-1 or MAT1-2 from a wild type haploid MAT1-1 MAT1-2 strain, resulting in MAT1-1；mat1-2, mat1-1；MAT1-2 strains that were self-sterile, yet able to cross to wild type testers and more importantly, to each other. These results indicated that differential deletion of MAT idiomorphs eliminates selfing ability of G. zeae, but the ability to outcross is retained. To our knowledge, this is the first report of complete conversion of fungal reproductive strategy from homothallic to heterothallic by targeted manipulation of MAT. Practically, this approach opens the door to simple and efficient procedures for obtaining sexual recombinants of G. zeae that will be useful for genetic analyses of mycotoxin production and other traits, such as ability to cause disease.

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