• Title/Summary/Keyword: Germination test

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Effects of Climatic Factors and Picking Time on Availability of Seeds of Chamaecyparis obtusa

  • Son, Seog-Gu;Kim, Chan-Soo;Hwang, Seok-In;Jeong, Jin-Heon;Choi, Wan-Yong
    • Journal of Korean Society of Forest Science
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    • v.97 no.2
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    • pp.135-139
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    • 2008
  • Seed production and germination could be influenced by some factors. The picking time and climate factors are regarded as the elements to obtain sound seeds. We have observed the seed productivity and germination of seeds from trees of Chamaecyparis obtusa selected in a clonal seed orchard. Depending on picking time, various shapes from liquid material, something jellied to the fully matured one were observed. Germination aspects varied throughout the test days. After 20days of seeding in a glass petri-dish, germinal apparatuses appeared from the all seeds which had been picked from after at the end of August. The highest germination rate of about 30% was observed from the seeds picked from $20^{th}$ of September and $10^{th}$ of October. Seed production was about two times higher in 2005 than in 2006 and the average germination rate was also higher in 2005. We have also analyzed the effects of climatic factors about two consecutive years on seed productivity. Among the climatic factors, monthly sum of temperature and of precipitation were the main factors for maturation of Chamaecyparis obtusa seeds.

Effects of Sprouting Treatment with Gibberellin to Germination and the Growth of Spring Barley (지벨레린 최아처리가 춘파대맥의 발아생육에 미치는 영향)

  • Won-Jong Ik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.7 no.1
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    • pp.117-121
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    • 1969
  • Germination and field test were made with the spring barley sprouted with gibberellin solution of 5 levels from 5 to 100 ppm. Temperatures of room for germination were classified as ordinary and low. Results obtained will be summarized as follows: 1. Sprouting with Gibberellin promoted the germination by two days in ordinary and three days in low temperature without reducing the germination rate. 2. The higher concentration of gibberellin showed the more promotion of growth in early stage. 3. No retardation of root-growth was found by gibberellin sprouting. 4. No promoting effects to germination by gibberellin sprouting was found in field tests. In high concentration, percentage of germination, number of spike and grain yields reduced remarkably. 5. More remarkable tendency of weak growth and longer duration of recovery were found in the higher concentration of gibberellin, but all plots recovered from weak growth at middle stage of plant growth. 6. In gibberellin concentration of 5 to 10 ppm, retardation of germination, weakness of early growth and reducing of spike number were not remarkable while length of spike and 1000 grain weight were increased. Therefore grain yields showed increasing tendency. 7. Promoting the germination may be expected but hastening of heading and maturing may not be available by gibberellin treatment in sprouting.

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Effects of Plant Growth Regulators on Seed Germination and Seedling Growth of Mountain Mulberry Seeds (Morus bombycis Koidz) (식물생장조절물질이 산뽕나무 종자의 발아 및 유묘생장에 미치는 영향)

  • Song, Min-Jeong;Kim, Kyung-Hoon;Hur, Young-Jin
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.19 no.1
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    • pp.101-109
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    • 2016
  • This is the fundamental research on restoring damaged vegetation areas in the vicinity of DMZ using local native plant species. This research is aimed at identifying effective plant growth regulators (PGR) for seed germination and seedling growth of mountain mulberry, and developing effective methods for managing its germination and growth. Mountain mulberry seeds were collected from the regions in the DMZ vicinity, and tests with seven treatments using four PGRs including $GA_3$, kinetin, ABA and NAA, were conducted. The germination rate was calculated in two different ways of both in a growth chamber and in a greenhouse after seven days observation, and the growth characters such as leaf width/length, seedling width/length and fresh/dry weight, have been surveyed in a greenhouse for three months. Although in the growth chamber the highest germination rate was shown at a group with the kinetin treatment, it was not significantly different to that of the control group. Groups with ABA or NAA presented relatively low germination rates. As for the greenhouse test, the germination rates of all groups ranged 20~30% without significant difference each other, the reason of which might be due to low absorption by the effect of a soil drench method used in this study. The entire growth characters with the treatments of $2.15mg{\cdot}L^{-1}$ of kinetin and $10mg{\cdot}L^{-1}$ of $GA_3$ were significantly different to the control. NAA treatment only showed better growth of seedling width compared to the control. Consequently, the most effective PGRs for the germination and growth of mountain mulberry near the DMZ was kinetin. Further research on examining the most effective concentration of them was needed.

Effect of Conidial Number and Nutrition on the Germination of Conidia in Septoria glycines (분생포자수 및 영양상태가 대두갈색무늬병균의 분생포자 발아에 미치는 영향)

  • Oh Jeung Haing;Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.23 no.1 s.58
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    • pp.61-67
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    • 1984
  • Conidial germination of Septoria glycines Hemmi, brown spot fungus of soybean, was studied by slide germination test. Poor conidial germination of S. glycines was observed on sterile distilled water, but potato dextrose agar(PDA) and distilled water floated with soybean leaf disc furnished a satisfactory medium for conidial germination. Exogenous supply of carbon source was essential for conidial germination, while phosphorous and potassium were not evident as that for carbon. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $5\times10^{-2}mol$. concentration of glucose. Germination was decreased with increment of conidial concentration and was almost completely suppressed in the density of 10,000 conidia per $mm^2$. It suggested existing a self-inhibitor(s). Non-washed conidia germinated more than washed conidia and this was obvious when the conidia density was over $2\times10^3$ conidia per $mm^2$ on the dry agar block.

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Analysis on Morphological Characters and Germination Conditions of Seeds in Ledum palustre var. diversipilosum (백산차(Ledum palustre var. diversipilosum) 종자의 형태적 특성 및 발아조건 분석)

  • Young-Min Choi;Seung-Ju Jo;Hyun-Ji Lee;Myung-Ja Kim;Jung-Won Sung;Jung-Won Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.23-23
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    • 2023
  • The Hairy labrador tea (Ledum palustre L. var. diversipilosum Nakai) is a evergreen small shrub, belonging to the Ericaceae and mainly distributed along the alpine areas of Hamgyeong-do in North Korea. Through seed physiological research on North Korean plants, we intend to obtain basic data for the development of mass propagation methods and use them for vegetation restoration. The internal and external morphological characters of the seed were observed using scanning electron microscopy or stereoscopy, and full seeds were selected through X-ray test. Seeds were cultured on a growth condition at 15/6℃ and 25/15℃ to examine the germination response according to temperature, and the number of germinated individuals was examined every 24 hours to calculate germination percentage and mean germination time. Low-temperature treatment (4℃) and hormone treatment (GA3) were performed to investigate the effect of dormancy breaking, and analysis of variance (ANOVA) was performed on the experimental results using SAS 9.4. The seeds of the Hairy labrador tea were elliptical-narrow in shape, and the epidermal cell wall pattern was reticulated. The final germination rate (FGP) was the highest at 58.9% under the condition of low temperature treatment at 25/15℃. The average number of days to germination (MGT) was the highest at 25.1 days without treatment at 15/6℃, and the average of the last germination days was confirmed to be about 13 days. As a result of low temperature treatment, it was confirmed that the final germination rate (FGP) increased by 43.3%. The significance of the final germination rate (FGP) was verified according to the pretreatment at 25/15℃.

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Allelopathic Activity and Determination of Allelochemicals from Sunflower (Helianthus annuus L.) Root Exudates - 1. Allelopathic and Autotoxic Effects of Sunflower Root Exudates (해바라기(Helianthus annuus L.) 근분비물질(根分泌物質)의 타감작용(他感作用) 및 타감물질(他感物質)의 동정(同定) - 1. 해바라기 근분비물질(根分泌物質)의 타감작용(他感作用) 및 자가억제작용(自家抑制作用))

  • Park, K.H.;Moody, K.;Kim, S.C.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.12 no.1
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    • pp.52-60
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    • 1992
  • There was no significant difference in the final germination percentages(Experiment 1-3) as affected by sunflower root exudates between the control and the treated for the test species. In general(Experiment 1), however, germination onset was delayed the treated bottles. Germination rate was, also, reduced for both radish(Raphanus sativus L.) and rice(Oryza sativa L.). Therefore, the germination index was low in the treated bottles but germination gradually increased with time in the greated bottles in all test species so that the final germination percentages were similar between treatments. The root exudates of sunflower had significant inhibitory effects(Experiment 1-3) on the lengths of the shoots and roots of all the test species. Fresh weight was also significantly reduced in all test species. Sunflower seedlings(Experiment 3) in the treated(with the XAD-4 resin column) were larger and healthier than those in the control (without XAD-4 resin column) because of the removal of allelochemicals. The fresh weight of sunflower seedlings was markedly inhibited by sunflower root exudates. These mean that sunflower probably is an autotoxic crop.

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Effect of Harvest Time on Seed Quality of Silage Corn Inbreds and Hybrids

  • Lee, Suk-Soon;Yun, Sang-Hee;Soo, Jung-Moon;Min, Hwang-Kee;Ryu, Si-Hwan;Park, Jong-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.5
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    • pp.361-367
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    • 2002
  • In order to determine the optimum harvest time for the seed production of inbreds and hybrids in silage corn, the ears of sib-pollinated 'KS5', 'KS7rhm', and 'Ga209' and cross-pollinated 'KS5' $\times$ 'KS6' (Suwon19), 'KS7 rhm' $\times$ 'KSl17' (Suwonok), and 'Ga209' $\times$ 'DB544'(Kwanganok) were harvested at the one-week intervals from 4 to 10 weeks after silking. The optimum harvest time for the seed production for 'KS5', 'KS5' $\times$ 'KS6', 'KS7 rhm', and 'KS7rhm' $\times$ 'KS117' was 7 weeks after silking considering both emergence rate and plumule growth in cold test. Although earlier harvested seeds showed similar germination rate as the seeds harvested at the optimum time at $25^{\circ}C$, their emergence rate were lower in cold test. Seed weight and $\alpha$-amylase activity of earlier harvested seeds were lower compared to those of seeds harvested at the optimum time, while leakage of total sugars and electrolytes were higher. However, the later harvested seeds showed lower germination rates at $25^{\circ}C$ and emergence rates in cold test probably due to the lower $\alpha$-amylase activity although they showed increased seed weight and reduced leakage of total sugars and electrolytes. In contrast, the emergence rate of 'Ga209' and 'Ga209' $\times$ 'DB544' in cold test increased up to 10 weeks after silking probably due to the increased seed weight and $\alpha$-amylase activity and reduced sugar and electrolyte leakages during the germination. The cross-pollinated F$_1$ hybrid seeds showed higher germination and emergence rates at $25^{\circ}C$ and in cold test, and higher plumule growth and $\alpha$-amylase activity compared to those of sib-pollinated inbreds.

Antifungal Activity of Narceine Methyl Ester and Narceine Isolated from Corydalis longipes Against Some Phytopathogenic Fungi

  • Chowdhury, Dibyendu;Maurya, S.;Pandey, M.B.;Pandey, V.B.;Sarma, B.K.;Singh, U.P.
    • Mycobiology
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    • v.33 no.4
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    • pp.206-209
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    • 2005
  • Narceine methyl ester and narceine are potent alkaloids which were isolated from Corydalis longipes were found effective in vitro at very low concentration, i.e., $100{\sim}500\;ppm$ against spore germination of some test plant pathogenic fungi (Alternaria solani, A. tagetica, Cercospora abelmoschi, Curvularia maculans, Erysiphe cichoracearum, E. pisi, Fusarium udum, Helminthosporium oryzae, H. penniseti, Ustilago cynodontis). Among the test, phytopathogens the spores of F. udum, C. maculans and H. penniseti were highly sensitive at 200 ppm. However, spores of E. pisi, A. solani and A. tagetica were less sensitive at low concentration followed by other test fungi. Most of the fungi showed zero or nearly zero percent spore germination at 400 and 500 ppm.

Seed Characteristics and Accelerating Method of Germination in Bupleurum falcatum (시호 종자의 특성 및 발아촉진 방법 연구)

  • Eunil, Lee;Seok Hyeon, Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.3
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    • pp.384-394
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    • 1996
  • The experiment was conducted to determine the seed characteristics and preferable methods to enhance the seed germination rate in Bupleurum falcatum. The optimum temperature for the seed germination of Bupleurum falcatum is 2$0^{\circ}C$. Any significant promoting effects were not found in seed germination with hormone treatments and physical methods. At 15$^{\circ}C$, prechilling combined with 50~200ppm of GA$_3$ treatment raised germination rate by 2 times of control ones. The most positive effect was observed in the treatment of 10$^{-2}$ ~10$^{-3}$ M potassium nitrate only at 15$^{\circ}C$ for 12 and 24 hours. The leachate of Bupleurum falcatum didn't inhibit the germination of Lactuca sativa, showing almost 100% of germination rate, which is suggested that no inhibitors contained in the seeds of Bupleurum falcatum. Observation of embryo conditions under stereoscopic microscope showed that the ratio of seeds with or without embryo is almost 50/50. The results suggested that the lower rate of germination in Bupleurum falcatum was caused by embryolessness of seeds.

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Effect of Presowing Seed Treatments on Germination and Seedling Emergence in Taraxacum platycarpum (민들레 종자의 파종전 처리가 발아율 및 입모율에 미치는 영향)

  • Jeon, Seung-Ho;Son, Daniel;Ryu, Yeong-Seop;Kim, Seok-Hyeon;Chung, Jong-Il;Kim, Min-Chul;Shim, Sang-In
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.1
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    • pp.9-14
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    • 2010
  • Several presowing seed treatments have been used to facilitate the seed germination and seedling emergence. The study was performed to design a model of presowing seed treatments in Taraxacum platycarpum. Germination and seedling growth responses to temperature, drying, chilling, $GA_3$ and priming were investigated to know the optimum germination conditions and presowing treatment. The optimum temperature condition for germination and seedling growth were $15{\sim}20^{\circ}C$ at dark condition. The seeds exposed to infra red light, however, did not germinate. The moisture content of seeds dried at $35^{\circ}C$ for 3.5 hours was returned to the level of unimbibed seeds. The germination of seeds dried under dark condition was better than those dried under infra red light. Prechilling treatment imposed for 6 weeks showed higher germination rate than $GA_3$ treatment or priming. In field test, the highest field emergence was observed in the seeds dried after prechilled for 6 weeks and followed by drying followed by $GA_3$ > low temperature > $KNO_3$ priming. Imbibition with 0.1 mM $GA_3$ solution for 2 hours then drying at 35 for 3.5 hour under dark condition was effective to the practical presowing treatment for Taraxacum seeds.