• 한국약용작물학회지
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• 제21권5호
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• pp.394-400
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• 2013

This study was carried out in order to examine the germination characteristics of Atractylodes japonica seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. Experiment was performed by two ways-temperature control (10, 15, 20, $25^{\circ}C$) and shading treatment (full sunlight, 35, 50, 75, 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60 days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 48 hours). Seeds of A. japonica were germinated well under temperature control (overall 85.3~100%), especially high temperature. And, the seeds pre-treated with LTW for 45 days germinated with a 100% success rate. Under the surveying shading treatment, the highest germination rate was 95.8% with DRT and overall 63.2~95.8%. Germination rate under seeds with WS was 63.2~7.8%. As a result of surveying the whole experiment, A. japonica seeds don't need to pre-treat cause of high germination, but if it is performed with LTW for a certain period of time, it would be more productive.

• 한국약용작물학회지
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• 제24권3호
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• pp.177-182
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• 2016

Background: Many Taraxacum spp. plants are widely used in medicine, but some of them have propagation problems, such as strong dormancy and poor germination rates. This study investigated the effects of temperature, gibberellic acid ($GA_3$), and potassium nitrate ($KNO_3$) on seed germination in Taraxacum ohwianum. Methods and Results: The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were $4.54{\pm}0.032mm$ and $0.97{\pm}0.029mm$, respectively. Various germination temperatures were tested (15, 20, 25, and $30^{\circ}C$), the optimum temperature for germination was found to be $20^{\circ}C$ (31.3%). High temperature ($30^{\circ}C$) resulted in non-typical seedlings (thickened and crumpled cotyledons, with a restricted root system). To increase the germination capacity, $GA_3$ (200, 500, and $1,000mg/{\ell}$) or $KNO_3$ (20, 50, 100, 200, and $500mg/{\ell}$) solutions were used as pre-soaking solutions instead of water. The $GA_3$ treatments increased the germination percentage and rate, but germination percentage was higher with the $KNO_3$ treatments. Under the $50-200mg/{\ell}\;KNO_3$ treatments, the germination percentage exceeded 80% after 12 days, and $50mg/{\ell}\;KNO_3$ was notably effective (91.2% after 15 days). Conclusions: T. ohwianum seeds showed improved germination at low temperatures. The best method for germination was presoaking in $50mg/{\ell}\;KNO_3$ for 24 h, in the dark at $4^{\circ}C$, and then incubating the germinated seed at $20^{\circ}C$ for 15 days.

• 한국환경생태학회지
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• 제12권1호
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• pp.9-13
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• 1998

조경용 소재로 그 관상 가치가 뛰어난 자생 갯까치수영의 종자는 저장기간이 길수록 발아력이 감소하며 그로 인한 보금에 어려움이 많았다. 본 실험에서는 우선 갯까치수영의 적정발아조건을 구명하고 프라이밍처리에 따른 발아상을 알아보기 위하여 실시하였다. 광과 온도에 따른 종자발아율을 알아본 결과 25$\circ $C의 온도조건과 광조건이 발아에 적정환경으로 이때 발아율과 발아속도는 각각 96.9%와 3.5일이었다. 20$\circ $C의 온도조건에서도 90%이상의 발아율을 보였으나 발아속도가 6.3일로 현저히 길어졌다. 저장기간이 길어졌을 때(300일) 75%까지 떨어졌던 종자의 발아율은 NaCl용액과 KNO$_{3}$ + K$_{3}$H$_{2}$PO 혼합용액을 프라이밍 처리한 결과 모든 처리농도에서 90% 이상으로 회복되었다. 여러 농도의 priming처리 후 발아속도에 차이가 있었는데, 처리농도가 각각 0.1M에서 0.5M로 높아질수록 발아속도가 길어지는 경향을 나타내었다.

• 원예과학기술지
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• 제34권3호
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• pp.372-382
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• 2016

In this study, we investigated the effect of seed collection stage and alternating temperature on the germination of Deutzia paniculata (Hydrangeaceae). The seeds were collected from naturally growing D. paniculata populations in Mt. Palgong, Gyeonsangbuk-do, Korea every two weeks over a three month period from August through October, 2014, totaling five collections. We examined seed characteristics such as seed size (length and width) and weight, and germination percentages for each collection stage under four different germination temperature regimes. We also examined germination after 30 and 60 days of cold moist stratification. Each collection phase had a different germination percentage and the lowest germination percentage was observed in the seeds from phase I (average GP of 15.7%). The germination percentage was similar at the later collection phases and in the higher temperatures suggesting that later collection times and higher temperatures are optimal for D. paniculata germination. We found that the optimum collection stage with high viability and germination percentage of for D. paniculata was during the middle of the September.

• 한국환경복원기술학회지
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• 제18권3호
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• pp.23-38
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• 2015

The study was initiated with Kentucky bluegrass (KB, Poa pratensis L.) to investigate germination and early establishment characteristics of new cultivars for a practical application to turfgrass establishment such as parks, athletic field and golf course etc. Fifteen cultivars were evaluated in different experiments. An alternative condition for a KB germination test required by International Seed Testing Association (ISTA) was applied in Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$ (natural conditions). Seed germinating vigor, germination speed and germination peak time were measured in both experiments. Significant differences were observed in seed germinating vigor, germination speed and germination peak time. Seed germinating vigor was variable with different environments and cultivars. It was 75.25 to 89.50% under ISTA conditions and 75.75 to 90.25% under natural conditions. There were considerable variations in early germination characteristics among KB cultivars according to different environments. Early germination characteristics showed that all cultivars were 3 to 5 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 50% to 80%, was much faster with 'Midnight II', 'Excursion' and 'Midnight' under ISTA conditions. But it was faster with 'Midnight II', 'Excursion' and 'Odyssey' under natural conditions. Differences was also observed in germination peak time with cultivars and growing conditions. It ranged 5.94 to 14.88 days under ISTA conditions and 4.71 to 13.06 days under natural conditions. Regardless of the environment conditions, the shortest cultivars were 'Odyssey' and 'Midnight II'. The longest ones were 'Nuglade' under ISTA condition and 'Moonlight' under natural conditions. Considering germinating vigor, germination speed and germination peak time, 'Midnight II', 'Excursion', 'Midnight', and 'Odyssey' were regarded as excellent cultivars under ISTA conditions in terms of early establishment characteristics, while 'Midnight II', 'Excursion', 'Odyssey', and 'Courtyard' under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for investigating the early germinating vigor, germination speed and germination peak time. Also, a proper cultivar selection and comprehensive site analysis for the growing environmental conditions should be done before turfgrass establishment such as golf course construction.

• 한국초지조사료학회지
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• 제38권4호
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• pp.325-330
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• 2018

본 연구는 퇴비단 내에서 소리쟁이 종자와 피 종자의 발아력 억제효과를 분석하기 위하여 염소 분 퇴비, 젖소 분 퇴비 그리고 한우 분 퇴비를 대상으로 하여 실험을 수행하였다. 소리쟁이 종자와 피 종자를 거즈에 싸서 퇴비단 내에 묻어놓고 1.5일, 3일, 10일, 20일 경과 후 종자를 회수한 뒤 발아력을 측정하였다. 실험 결과 소리쟁이 종자와 피 종자의 발아율은 가축 분의 종류와 퇴비화 방법에 관계없이 퇴비단의 온도와 직접적인 상관관계를 가지는 것으로 나타났다. 온도가 $60{\sim}70^{\circ}C$인 조건의 퇴비단에서 1.5일 동안 머무른 소리쟁이 종자와 피 종자는 발아력을 완전히 상실하였다. 반면에 퇴비단 최고 온도가 $51^{\circ}C$ 이하인 퇴비단에서는 소리쟁이 종자와 피 종자가 퇴비단 내에서 20일간 머무른 후에도 10 % 정도의 발아력을 유지하였다. 이상의 결과를 종합해보면, 소리쟁이 종자와 피 종자의 발아율을 억제하기 위해서는 가축 분 퇴비화단계에서 최소 $55^{\circ}C$ 이상의 온도를 3일 이상 유지하는 것이 좋다. 또한 퇴비단 온도가 $60{\sim}70^{\circ}C$ 정도 상승한다면 약 1.5일 정도 체류하는 것으로도 소리쟁이 종자와 피 종자의 발아력을 억제할 수 있을 것이다. 퇴비화 방법을 기준으로 보면, 호기적 퇴비화 방법이 퇴비단의 온도상승기간이 더 짧았다. 따라서 호기적 퇴비화방법을 적용하는 것이 소리쟁이 종자와 피 종자의 발아력을 억제하는데 더 효율적일 것이다.

• The Korean Journal of Ecology
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• 제17권3호
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• pp.367-378
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• 1994

돌피(Echinochloa crus-galli (L.) Beauv.) 종자는 산포 직후 상온에서 휴면상태로 발아가 억제되고 있으나 저장기간이 지나면서 휴면이 타파되어 7~17개월후에 16~40$^{\circ}C$의 온도에서 85~95%의 최대발아율을 나타내었다. 돌피종자가 10~70% 발아하는데 요구되는 적산온도는 539~1,279Kh (degree kelvinXhours)이며 적산온도 분포함수인 F($\theta$)=1-[3D-3($\theta$-m+D)3+1]-1/2와 일치하였고 m과 D의 값은 각각 935Kh와 555kh로 나타났다. 2$^{\circ}C$에서 냉습처리한 후, 16~40$^{\circ}C$의 항온 조건하에 4$^{\circ}C$ 간격으로 처리한 돌피종자는 최종발아율과 발아속도가 대조구에 비해 증가하였다. IT regime에 있어서는 12$^{\circ}C$부터 발아속도가 증가하여 24$^{\circ}C$에서 최종발아율이 80%가 되었으며, DT regime은 24$^{\circ}C$에서 0$^{\circ}C$로 하강하는 동안 12$^{\circ}C$에서부터 발아가 시작되었으며(발아율 10%), 8$^{\circ}C$에서 20%의 최종발아율을 나타내었고 4$^{\circ}C$부터는 2차 휴면이 유도되었다. 돌피종자는 비교적 넓은 발아기능 온도범위를 가지고 있으며 개체군내의 발아시기조절에 의해 다양한 환경변화에서도 잘 적응할 수 있으며 변온과 겨울의 저온에 의해 휴면이 타파되고 봄의 기온상승과 함께 발아하는 봄발아형 종자임을 알 수 있었다.

• 원예과학기술지
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• 제34권2호
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• pp.236-241
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• 2016

The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.288-288
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• 2017

The seeds of soybean (Glycine max) were treated with different storage temperature for this study. The seeds of 3 accessions of soybean varieties in aluminum foil pack were used as materials. Storage temperature applied were $-18^{\circ}C$ and room temperature and seed banking (input after 3 days from output) frequencies were every 1, 6, and 12 month respectively for 9 years of storage period. As results seed banking frequency no affected to germination rate and seed moisture contents at $-18^{\circ}C$ storage room for seeds of soybean after 9 years. Germination rate of soybean seeds was changed from 96.2 % to 95.6 % averagely after 9 years of freezing ($-18^{\circ}C$) storage period. There were no differences in decreasing rate by number of seed banking frequency in soybean seeds. On the other hand, at room temperature germination rate of soybean seeds was decreased from 96.2 % to 27.3 % after 9 years which was decreased sharply to 55 % of initial viability after 6 years. The average rate of annual decrease of germination rate in soybean seeds was 38 % of initial viability at room temperature. Initial moisture contents of soybean seeds were 7.3 % and changed to 7.1 % at $-18^{\circ}C$ while it changed from 7.4 % to 7.0 % at room temperature after 9 years of storage period.

• The Korean Journal of Ecology
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• 제25권6호
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• pp.389-393
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• 2002

Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.