• 한국자원식물학회지
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• 제21권2호
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• pp.144-147
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• 2008

자연상태에서 발아율이 낮은 나도생강은 멸종위기에 처해 1997년 산림청에서 보호종으로 지정되었다. 나도생강의 종자 번식률 향상을 위해 먼저 광, 온도에 따른 발아특성을 알아보았다. 나도생강 종자는 광조건이 암조건보다 발아율이 높고 생육 상태가 양호하였다. 온도별 발아율을 조사한 결과는 $20^{\circ}C$에서 발아시키는 것이 다른 온도조건보다 발아율이 높았다. 발아율 증진을 위해 종자 전처리(priming)와 저장방법을 달리하여 실험을 수행하였다. 종자 전처리제로는 1%농도의 KOH와 NaOH용액 30분침지가 효율이 높았고, 1% NaOCl 와 100mg/L GA3용액은 대조구 보다 낮은 발아율을 보였다. 종자저장처리는 건조저장와 습윤저장를 하였으며 저장온도는 두 처리 모두 $4^{\circ}C$에서 실시하였다. 습윤저장이 건조저온저장보다 탁월한 결과를 보였으며, 90일간 습윤저장 한 경우 발아율이 95.3%로 건조저장 보다 55.3% 높았다.

• 한국환경농학회지
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• 제21권3호
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• pp.202-207
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• 2002

참박과 호박 채종종자의 발아율을 증진시킬 수 있는 종자처리 방법을 확립하고자 저선량 감마선을 1$\sim$20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 그 효과는 종자상태에 따라 다르게 나타났다. 참박 70$\sim$80% 발아율종자의 발아는 4$\sim$20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80$\sim$90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70$\sim$80%와 80$\sim$90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70$\sim$80% 발아율종자는 2 Gy 조사에서, 80$\sim$90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70$\sim$80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy 조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다.

• Journal of Plant Biology
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• 제14권2호
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• pp.1-6
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• 1971

When germination beds of carrot seeds were treated with either 0.01M or 0.05M concentrations of Ca(NO3)2, CaSO4, MgSO4, K2SO4 and KH2PO4, an acceleration in the germination rate was observed in the groups treated with 0.01M KH2PO4 and 0.05M MgSO4 and 0.05M Ca(NO2)3. In earlier work by the author with acetone a similar result was observed and reported. The pH range in these experiments was maintained between 5.0 and 6.0. It was found that the groups treated with 0.05M K2SO4, 0.05M Ca(NO3)2, 0.05M Ca(NO3)2, 0.05M MgSO4, 0.01M KH2PO4, 0.01M Ca(NO3)2 germinated earlier than the control group. The acceleration of the germintion rate varied with the inorganic compounds used in the following descending order; 0.01M KH2PO4, 0.05M Ca(NO3)2, 0.05M K2SO4, 0.05M CaSO4 and 0.05M KH2PO4. As a result of these expriments, it occurs to the author that in the germination of carrot seeds some inorganic compounds appear to activate the osmotic function of carrot seeds causing acceleration in the germination rate.

• The Korean Journal of Ecology
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• 제18권3호
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• pp.353-366
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• 1995

To study the relationship between the germination response and geographical distribution, the seeds of Rumex acetocella were collected from nine sites (Taegwallyong, Alps lodge, Kans'ong, Sokch'o, Chumunjin, Suwon, Taechon, Sukgok, Mokpo) of different latitudes and altitudes. The populations of Rumex acetocella were subdivided into four groups based on the characteristics of the seed germination. The first group of Rumex acetocella (Taegwallyong, Alps lodge) germinated rapidly in early spring, the second group (Suwon, Teachon) in late summer through early autumn, and the third group(Sukgok, Mokpo) slowly in late autuman with various conditions. The thermal time values needed for the germination rate of 10% to 60% for these three groups indicated that the germination rate was low at the lower latitude, while it was increased as the latitude elevated. The population of the fouth group (Kansong, Sokcho, Chumunjin) showed the narrow optimum temperature range of germination response and the highest thermal time value to germinate 10% to 30% of seeds. The seeds in the high latitude region with relatively mild winter germinated synchronously in early autumn. These different germination responses of Rumex acetocella to geographic variations were thought to be an ecological strategy to survive in different environmental conditions.

• 한국자원식물학회지
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• 제27권3호
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• pp.256-262
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• 2014

Viscum album var. coloratum (mistletoe) is considered as one of the endangered plant species in Korea. Our objective is to restore its population and multiplication of plant by ex situ method. In this research we explored the maximum germination (in vitro) of freshly harvested and stored seeds of mistletoe collected in different time intervals. Ex vitro germination after artificial inoculation on the branches of Prunus mume in different physiological conditions was also monitored. The research revealed that the lately harvested seeds (Feb. and March 2014) were superior over early harvested seeds (Nov. 2013 and Jan. 2014) of mistletoe due to the higher percentage of germination (above 93%). According to the data, it is also revealed that the survival and germination rate of mistletoe seeds decreased with the increase in storage duration. In ex vitro germination, the fluctuated temperature of a glass house in natural condition enhanced (four fold) the rate of germination on the branches of Prunus mume than the constant temperature condition in the glass house.

• 한국환경보건학회지
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• 제27권3호
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• pp.21-26
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• 2001

This study was performed to define the physicochemical characteristics of food waste and food wastewater, and to find the effect of salinity variation experiment, the samples of 1, 2, 3 and 4 were prepared by the salinity of 1.0%, 1.5%, 2.0% and 2.5%, respectively. In experiment, aerobic composting reactors were operated by the model which was composed of half an hour's stirring and 2 hour's aeration per day, for 45 days. Seeds of vegetables of Chinese cabbage and red pepper were seeded at compost-free control, compost 1, 2, 3 and compost 4 for the sake of investigation of germination rate and growth rate of those. The followings are the conclusions that were derived from this study. 1. In food waste, the moisture content was 60%, organic compound content was 95%, total carbon was 47.5%, total nitrogen was 1.6%(therefore, C/N ratio was 30). The values of pH were 4.0 and 3.8 the values of salinity were 0.9% and 1.2%, and the values of conductivity were 7.8 mS/cm and 18.0 mS/cm, respectively. 2. In salinity experiment, the reduction rate of volume was increased(40%) when the salinity was decreased(1.0%). Also, the reduction rate of mass was increased(52%) when the salinity was decreased(1.0%). This fact denotes that salinity hinders the process of composting. 3. Germination rate and growth rate of Chinese cabbage are more excellent than those of red pepper(97.14%, 5.2:2.5 cm). 4. In Chinese cabbage, germination rate and growth rate at compost with the lowest salinity are more excellent than those at compost-free control(97:94%, 5.2:4.5 cm).

• 환경생물
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• 제22권1호
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• pp.200-205
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• 2004

유독성 와편모조류 Alexandrium tamarense 휴면시스트의 발아율을 여러 환경조건하에서 측정하여 각각의 환경요인과 휴면시스트의 발아율과의 관계를 밝히고자 하였다. 마산만에서 채집한 해저니로부터 휴면시스트를 분리하여 실험실 배양을 통해 온도, 광조건의 유무, 염분 그리고 저니층의 깊이에 따른 발아율을 각각 조사하였다. 온도별 발아율은 $15^{\circ}C$에서 가장 높았으며 $10^{\circ}C$에서도 높은 발아율이 기록된 반면, $20^{\circ}C$와 $25^{\circ}C$에서는 거의 발아되지 않았다. 광조건의 유무에 따른 발아율은 명조건과 암조건에서 두드러진 차이를 나타내지 않았다. 염분에 따른 발아율도 큰 차이를 보이지 않았다. 저니층별 발아율은 3월에 분리된 휴면시스트의 경우는 표층이 저층보다 낮은 발아율을 보였지만, 11월에는 표층이 저층보다 높은 발아율을 보여 시기별로 대조적인 결과를 얻었다. 따라서, 마산만에서 분리된 A. tamarense 휴면시스트의 발아는 수온의 변화에 가장 큰 영향을 받는다고 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.23-23
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• 2023

The Hairy labrador tea (Ledum palustre L. var. diversipilosum Nakai) is a evergreen small shrub, belonging to the Ericaceae and mainly distributed along the alpine areas of Hamgyeong-do in North Korea. Through seed physiological research on North Korean plants, we intend to obtain basic data for the development of mass propagation methods and use them for vegetation restoration. The internal and external morphological characters of the seed were observed using scanning electron microscopy or stereoscopy, and full seeds were selected through X-ray test. Seeds were cultured on a growth condition at 15/6℃ and 25/15℃ to examine the germination response according to temperature, and the number of germinated individuals was examined every 24 hours to calculate germination percentage and mean germination time. Low-temperature treatment (4℃) and hormone treatment (GA3) were performed to investigate the effect of dormancy breaking, and analysis of variance (ANOVA) was performed on the experimental results using SAS 9.4. The seeds of the Hairy labrador tea were elliptical-narrow in shape, and the epidermal cell wall pattern was reticulated. The final germination rate (FGP) was the highest at 58.9% under the condition of low temperature treatment at 25/15℃. The average number of days to germination (MGT) was the highest at 25.1 days without treatment at 15/6℃, and the average of the last germination days was confirmed to be about 13 days. As a result of low temperature treatment, it was confirmed that the final germination rate (FGP) increased by 43.3%. The significance of the final germination rate (FGP) was verified according to the pretreatment at 25/15℃.

• The Plant Pathology Journal
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• 제22권1호
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• pp.11-15
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• 2006

Five seed samples of carrot were tested to detect Alternaria spp. by blotter method. A. alternata and A. radicina were detected from all the seed samples as high as $25.8-70.5\%$ and $37.5-63.5\%$, respectively. A. dauci was detected from four seed samples as low as $0.5-7.5\%$. The three Alternaria spp. were detected from the pericarp and the seed coat and endosperm of the carrot seeds but not from the embryo by component plating test. A. alternata and A. radicina were much more detected from the pericarp than the seed coat and endosperm. A. dauci was detected from the pericarp and the seed coat and endosperm at similar rate. The seed sample which was most severely infected with A. radicina showed the lowest rate of germination in the test on top of paper (TP). In the TP test, differences in total infection rate of A. radicina and A. dauci of the seed samples were very closely correlated with those in incidence of seedling rot on the seed samples. However, there was no correlation between infection rate of A. alternata and rate of germination or seedling rot of the seed samples. Soil test for seedling growth revealed that there was no correlation between differences in total infection rate of A. radicina and A. dauci and those in rate of normal seedlings of the seed samples.

• 생명과학회지
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• 제10권6호
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• pp.598-604
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• 2000

This study was conducted to investigate the effect of simulated acid rain (SAR) on germination, growth, acid buffering capacity and nutrient leaching in Impatiens balsamina L. and Tagetes patula L.. In both species, germination was not inhibited at pH 4.0, but the germination rate decreased at the lower pH of 3.0, showing higher rate in Inpatiens balsamina L. than Tagetes patula L.. As the pH decreases, the growth of radicle was markedly decreased than that of hypocotyl in both species. The plant height, root length, leaf area, total dry weight, relative growth rate and net assimilation rate were inhibited by SAR. The acid buffering capacity in the leaves were increased at pH 4.0, on the other hand, it was shown a tendency to decrease at pH 2.0 in both species. As the pH levels decreased from 5.6 to 2.0, the nutrient leaching from leaves was significantly increased in both species. Based on the results, there are a great difference in the responses to SAR between the two species. In general, Tagetes patula L. represented a higher tolerance to SAR than Impatiens balsamina L.. These results suggested that interspecific variation in the acid buffering capacity and nutrient leaching from leaves may be responsible for the interspecific susceptibility to SAR.